Journal: BMC Immunology

Article Title: Human neutrophil migration and activation by BJcuL, a galactose binding lectin purified from Bothrops jararacussu venom

doi: 10.1186/1471-2172-12-10

Figure Lengend Snippet: BJcuL is evenly distributed on the surface of human neutrophils . Isolated human neutrophils (10 6 cells/ml) were adhered to coverslips coated with Biobond, fixed and then incubated for 45 min at RT with (A) PBS; (B) Biotinylated BJcuL (10 μg/ml); (C) Biotinylated BJcuL pre-incubated with Gal 2 mM. (D) Biotinylated BJcuL pre-incubated with GlcNAc 2 mM. Cells were examined by fluorescence microscopy after reaction with streptavidin-FITC.

Article Snippet: Neutrophils (10 6 cells/ml) were placed on coverslips coated with Biobond (EM Science, Fort Washington, PA, USA), fixed with 2% formaldehyde at room temperature (RT) for 20 min and incubated with biotinylated BJcuL (10 μg/ml) at 37°C for 1 hr.

Techniques: Isolation, Incubation, Fluorescence, Microscopy