between Search Results


balb cbyj mice  (ATCC)
90
ATCC balb cbyj mice
Balb Cbyj Mice, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/between/Mouse+(Balb%2Fc)+hybridoma+between+S2+myeloma+and+splenocytes+DCGM4/pm17675497-89-3-11
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balb cbyj mice - by Bioz Stars, 2026-09
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antibodies against hoip  (Boster Bio)
91
Boster Bio antibodies against hoip
ISOF inhibited the expression of LUBAC in MLGs. (A) The protein levels <t>of</t> <t>SHARPIN</t> in the MLGs stimulated with TGF-β1 was analyzed by western blot. The western blot representative strip was listed on the right of figure. (B) The protein levels of HOIL-1L in the MLGs stimulated with TGF-β1 was analyzed by western blot. The western blot representative strip was listed on the right of figure. (C) The protein levels of <t>HOIP</t> in the MLGs stimulated with TGF-β1 was analyzed by western blot. The western blot representative strip was listed on the right of figure. ANOVA was used for western blotting statistics (SHARPIN, n=8; HOIL-1L, n=5; HOIP, n=10). ANOVA, analysis of variance; DXM, dexamethasone; GAPDH, Glyceraldehyde-3-phosphate dehydrogenase; HOIL-1L, heme oxidation IRP2 ubiquitin ligase 1L; HOIP, HOIL-1L interacting protein; HOIPIN-8, HOIP inhibitor-8; ISOF, isoforskolin; LUBAC, linear ubiquitin chain assembly complex; MLGs, mice lung fibroblasts; PFD, pirfenidone; SHARPIN, shank-associated RH domain interaction protein; TGF-β1, transforming growth factor-β1.
Antibodies Against Hoip, supplied by Boster Bio, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/between/Anti-RNF31%2FHOIP+Antibody+Picoband/pmc12875815-66-30-42
Average 91 stars, based on 1 article reviews
antibodies against hoip - by Bioz Stars, 2026-09
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managing exchanges between edge gateways in a cloud environment to support a private network connection  (VMware Inc)
90
VMware Inc managing exchanges between edge gateways in a cloud environment to support a private network connection
ISOF inhibited the expression of LUBAC in MLGs. (A) The protein levels <t>of</t> <t>SHARPIN</t> in the MLGs stimulated with TGF-β1 was analyzed by western blot. The western blot representative strip was listed on the right of figure. (B) The protein levels of HOIL-1L in the MLGs stimulated with TGF-β1 was analyzed by western blot. The western blot representative strip was listed on the right of figure. (C) The protein levels of <t>HOIP</t> in the MLGs stimulated with TGF-β1 was analyzed by western blot. The western blot representative strip was listed on the right of figure. ANOVA was used for western blotting statistics (SHARPIN, n=8; HOIL-1L, n=5; HOIP, n=10). ANOVA, analysis of variance; DXM, dexamethasone; GAPDH, Glyceraldehyde-3-phosphate dehydrogenase; HOIL-1L, heme oxidation IRP2 ubiquitin ligase 1L; HOIP, HOIL-1L interacting protein; HOIPIN-8, HOIP inhibitor-8; ISOF, isoforskolin; LUBAC, linear ubiquitin chain assembly complex; MLGs, mice lung fibroblasts; PFD, pirfenidone; SHARPIN, shank-associated RH domain interaction protein; TGF-β1, transforming growth factor-β1.
Managing Exchanges Between Edge Gateways In A Cloud Environment To Support A Private Network Connection, supplied by VMware Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/between/managing+exchanges+between+edge+gateways+in+a+cloud+environment+to+support+a+private+network+connection/us12126598-1-14-31
Average 90 stars, based on 1 article reviews
managing exchanges between edge gateways in a cloud environment to support a private network connection - by Bioz Stars, 2026-09
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silicon probes (2 shanks × 32 channels) with spacing between shanks and spacing between channels (h2)  (Cambridge NeuroTech)
90
Cambridge NeuroTech silicon probes (2 shanks × 32 channels) with spacing between shanks and spacing between channels (h2)
ISOF inhibited the expression of LUBAC in MLGs. (A) The protein levels <t>of</t> <t>SHARPIN</t> in the MLGs stimulated with TGF-β1 was analyzed by western blot. The western blot representative strip was listed on the right of figure. (B) The protein levels of HOIL-1L in the MLGs stimulated with TGF-β1 was analyzed by western blot. The western blot representative strip was listed on the right of figure. (C) The protein levels of <t>HOIP</t> in the MLGs stimulated with TGF-β1 was analyzed by western blot. The western blot representative strip was listed on the right of figure. ANOVA was used for western blotting statistics (SHARPIN, n=8; HOIL-1L, n=5; HOIP, n=10). ANOVA, analysis of variance; DXM, dexamethasone; GAPDH, Glyceraldehyde-3-phosphate dehydrogenase; HOIL-1L, heme oxidation IRP2 ubiquitin ligase 1L; HOIP, HOIL-1L interacting protein; HOIPIN-8, HOIP inhibitor-8; ISOF, isoforskolin; LUBAC, linear ubiquitin chain assembly complex; MLGs, mice lung fibroblasts; PFD, pirfenidone; SHARPIN, shank-associated RH domain interaction protein; TGF-β1, transforming growth factor-β1.
Silicon Probes (2 Shanks × 32 Channels) With Spacing Between Shanks And Spacing Between Channels (H2), supplied by Cambridge NeuroTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
silicon probes (2 shanks × 32 channels) with spacing between shanks and spacing between channels (h2) - by Bioz Stars, 2026-09
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fixed-gradient (12 %) precast polyacrylamide gels  (GenScript corporation)
90
GenScript corporation fixed-gradient (12 %) precast polyacrylamide gels
ISOF inhibited the expression of LUBAC in MLGs. (A) The protein levels <t>of</t> <t>SHARPIN</t> in the MLGs stimulated with TGF-β1 was analyzed by western blot. The western blot representative strip was listed on the right of figure. (B) The protein levels of HOIL-1L in the MLGs stimulated with TGF-β1 was analyzed by western blot. The western blot representative strip was listed on the right of figure. (C) The protein levels of <t>HOIP</t> in the MLGs stimulated with TGF-β1 was analyzed by western blot. The western blot representative strip was listed on the right of figure. ANOVA was used for western blotting statistics (SHARPIN, n=8; HOIL-1L, n=5; HOIP, n=10). ANOVA, analysis of variance; DXM, dexamethasone; GAPDH, Glyceraldehyde-3-phosphate dehydrogenase; HOIL-1L, heme oxidation IRP2 ubiquitin ligase 1L; HOIP, HOIL-1L interacting protein; HOIPIN-8, HOIP inhibitor-8; ISOF, isoforskolin; LUBAC, linear ubiquitin chain assembly complex; MLGs, mice lung fibroblasts; PFD, pirfenidone; SHARPIN, shank-associated RH domain interaction protein; TGF-β1, transforming growth factor-β1.
Fixed Gradient (12 %) Precast Polyacrylamide Gels, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/between/surepagetm+precast+gels+with+a+linear+gradient+between+4++20/pmc11674787-181-6-12
Average 90 stars, based on 1 article reviews
fixed-gradient (12 %) precast polyacrylamide gels - by Bioz Stars, 2026-09
90/100 stars
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between-population linkage disequilibrium measure d i s 2  (Ohta s Isan Co Ltd)
90
Ohta s Isan Co Ltd between-population linkage disequilibrium measure d i s 2
ISOF inhibited the expression of LUBAC in MLGs. (A) The protein levels <t>of</t> <t>SHARPIN</t> in the MLGs stimulated with TGF-β1 was analyzed by western blot. The western blot representative strip was listed on the right of figure. (B) The protein levels of HOIL-1L in the MLGs stimulated with TGF-β1 was analyzed by western blot. The western blot representative strip was listed on the right of figure. (C) The protein levels of <t>HOIP</t> in the MLGs stimulated with TGF-β1 was analyzed by western blot. The western blot representative strip was listed on the right of figure. ANOVA was used for western blotting statistics (SHARPIN, n=8; HOIL-1L, n=5; HOIP, n=10). ANOVA, analysis of variance; DXM, dexamethasone; GAPDH, Glyceraldehyde-3-phosphate dehydrogenase; HOIL-1L, heme oxidation IRP2 ubiquitin ligase 1L; HOIP, HOIL-1L interacting protein; HOIPIN-8, HOIP inhibitor-8; ISOF, isoforskolin; LUBAC, linear ubiquitin chain assembly complex; MLGs, mice lung fibroblasts; PFD, pirfenidone; SHARPIN, shank-associated RH domain interaction protein; TGF-β1, transforming growth factor-β1.
Between Population Linkage Disequilibrium Measure D I S 2, supplied by Ohta s Isan Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/between/between+population+linkage+disequilibrium+measure+d+i+s+2/pmc04978898-42-17-12
Average 90 stars, based on 1 article reviews
between-population linkage disequilibrium measure d i s 2 - by Bioz Stars, 2026-09
90/100 stars
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between-group manipulation of the task  (Schill Seilacher GmbH)
90
Schill Seilacher GmbH between-group manipulation of the task
ISOF inhibited the expression of LUBAC in MLGs. (A) The protein levels <t>of</t> <t>SHARPIN</t> in the MLGs stimulated with TGF-β1 was analyzed by western blot. The western blot representative strip was listed on the right of figure. (B) The protein levels of HOIL-1L in the MLGs stimulated with TGF-β1 was analyzed by western blot. The western blot representative strip was listed on the right of figure. (C) The protein levels of <t>HOIP</t> in the MLGs stimulated with TGF-β1 was analyzed by western blot. The western blot representative strip was listed on the right of figure. ANOVA was used for western blotting statistics (SHARPIN, n=8; HOIL-1L, n=5; HOIP, n=10). ANOVA, analysis of variance; DXM, dexamethasone; GAPDH, Glyceraldehyde-3-phosphate dehydrogenase; HOIL-1L, heme oxidation IRP2 ubiquitin ligase 1L; HOIP, HOIL-1L interacting protein; HOIPIN-8, HOIP inhibitor-8; ISOF, isoforskolin; LUBAC, linear ubiquitin chain assembly complex; MLGs, mice lung fibroblasts; PFD, pirfenidone; SHARPIN, shank-associated RH domain interaction protein; TGF-β1, transforming growth factor-β1.
Between Group Manipulation Of The Task, supplied by Schill Seilacher GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/between/between+group+manipulation+of+the+task/pmc05623132-120-11-5
Average 90 stars, based on 1 article reviews
between-group manipulation of the task - by Bioz Stars, 2026-09
90/100 stars
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differentiated (d-rdt) allowing differentiation between igg and igm  (Wondfo USA)
90
Wondfo USA differentiated (d-rdt) allowing differentiation between igg and igm
ISOF inhibited the expression of LUBAC in MLGs. (A) The protein levels <t>of</t> <t>SHARPIN</t> in the MLGs stimulated with TGF-β1 was analyzed by western blot. The western blot representative strip was listed on the right of figure. (B) The protein levels of HOIL-1L in the MLGs stimulated with TGF-β1 was analyzed by western blot. The western blot representative strip was listed on the right of figure. (C) The protein levels of <t>HOIP</t> in the MLGs stimulated with TGF-β1 was analyzed by western blot. The western blot representative strip was listed on the right of figure. ANOVA was used for western blotting statistics (SHARPIN, n=8; HOIL-1L, n=5; HOIP, n=10). ANOVA, analysis of variance; DXM, dexamethasone; GAPDH, Glyceraldehyde-3-phosphate dehydrogenase; HOIL-1L, heme oxidation IRP2 ubiquitin ligase 1L; HOIP, HOIL-1L interacting protein; HOIPIN-8, HOIP inhibitor-8; ISOF, isoforskolin; LUBAC, linear ubiquitin chain assembly complex; MLGs, mice lung fibroblasts; PFD, pirfenidone; SHARPIN, shank-associated RH domain interaction protein; TGF-β1, transforming growth factor-β1.
Differentiated (D Rdt) Allowing Differentiation Between Igg And Igm, supplied by Wondfo USA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/between/differentiated++d+rdt++allowing+differentiation+between+igg+and+igm/pmc07309836-107-37-23
Average 90 stars, based on 1 article reviews
differentiated (d-rdt) allowing differentiation between igg and igm - by Bioz Stars, 2026-09
90/100 stars
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ha regenyal starter - 32 mg/2 highly-purified ha, with molecular weight between 800-1.200 kda  (Regenyal Laboratories)
90
Regenyal Laboratories ha regenyal starter - 32 mg/2 highly-purified ha, with molecular weight between 800-1.200 kda
ISOF inhibited the expression of LUBAC in MLGs. (A) The protein levels <t>of</t> <t>SHARPIN</t> in the MLGs stimulated with TGF-β1 was analyzed by western blot. The western blot representative strip was listed on the right of figure. (B) The protein levels of HOIL-1L in the MLGs stimulated with TGF-β1 was analyzed by western blot. The western blot representative strip was listed on the right of figure. (C) The protein levels of <t>HOIP</t> in the MLGs stimulated with TGF-β1 was analyzed by western blot. The western blot representative strip was listed on the right of figure. ANOVA was used for western blotting statistics (SHARPIN, n=8; HOIL-1L, n=5; HOIP, n=10). ANOVA, analysis of variance; DXM, dexamethasone; GAPDH, Glyceraldehyde-3-phosphate dehydrogenase; HOIL-1L, heme oxidation IRP2 ubiquitin ligase 1L; HOIP, HOIL-1L interacting protein; HOIPIN-8, HOIP inhibitor-8; ISOF, isoforskolin; LUBAC, linear ubiquitin chain assembly complex; MLGs, mice lung fibroblasts; PFD, pirfenidone; SHARPIN, shank-associated RH domain interaction protein; TGF-β1, transforming growth factor-β1.
Ha Regenyal Starter 32 Mg/2 Highly Purified Ha, With Molecular Weight Between 800 1.200 Kda, supplied by Regenyal Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/between/ha+regenyal+starter+++32+mg+2+highly+purified+ha++with+molecular+weight+between+800+1+200+kda/10__1007_slash_s11332___017___0384___3-3657-21-22
Average 90 stars, based on 1 article reviews
ha regenyal starter - 32 mg/2 highly-purified ha, with molecular weight between 800-1.200 kda - by Bioz Stars, 2026-09
90/100 stars
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known interactions between human orthologs  (GeneGo Inc)
90
GeneGo Inc known interactions between human orthologs
ISOF inhibited the expression of LUBAC in MLGs. (A) The protein levels <t>of</t> <t>SHARPIN</t> in the MLGs stimulated with TGF-β1 was analyzed by western blot. The western blot representative strip was listed on the right of figure. (B) The protein levels of HOIL-1L in the MLGs stimulated with TGF-β1 was analyzed by western blot. The western blot representative strip was listed on the right of figure. (C) The protein levels of <t>HOIP</t> in the MLGs stimulated with TGF-β1 was analyzed by western blot. The western blot representative strip was listed on the right of figure. ANOVA was used for western blotting statistics (SHARPIN, n=8; HOIL-1L, n=5; HOIP, n=10). ANOVA, analysis of variance; DXM, dexamethasone; GAPDH, Glyceraldehyde-3-phosphate dehydrogenase; HOIL-1L, heme oxidation IRP2 ubiquitin ligase 1L; HOIP, HOIL-1L interacting protein; HOIPIN-8, HOIP inhibitor-8; ISOF, isoforskolin; LUBAC, linear ubiquitin chain assembly complex; MLGs, mice lung fibroblasts; PFD, pirfenidone; SHARPIN, shank-associated RH domain interaction protein; TGF-β1, transforming growth factor-β1.
Known Interactions Between Human Orthologs, supplied by GeneGo Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/between/known+interactions+between+human+orthologs/10__1186_slash_1471___213x___10___120-141-15-17
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known interactions between human orthologs - by Bioz Stars, 2026-09
90/100 stars
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equilibria and assessment of processes between air, water, sediments and biota  (Verlag GmbH)
90
Verlag GmbH equilibria and assessment of processes between air, water, sediments and biota
ISOF inhibited the expression of LUBAC in MLGs. (A) The protein levels <t>of</t> <t>SHARPIN</t> in the MLGs stimulated with TGF-β1 was analyzed by western blot. The western blot representative strip was listed on the right of figure. (B) The protein levels of HOIL-1L in the MLGs stimulated with TGF-β1 was analyzed by western blot. The western blot representative strip was listed on the right of figure. (C) The protein levels of <t>HOIP</t> in the MLGs stimulated with TGF-β1 was analyzed by western blot. The western blot representative strip was listed on the right of figure. ANOVA was used for western blotting statistics (SHARPIN, n=8; HOIL-1L, n=5; HOIP, n=10). ANOVA, analysis of variance; DXM, dexamethasone; GAPDH, Glyceraldehyde-3-phosphate dehydrogenase; HOIL-1L, heme oxidation IRP2 ubiquitin ligase 1L; HOIP, HOIL-1L interacting protein; HOIPIN-8, HOIP inhibitor-8; ISOF, isoforskolin; LUBAC, linear ubiquitin chain assembly complex; MLGs, mice lung fibroblasts; PFD, pirfenidone; SHARPIN, shank-associated RH domain interaction protein; TGF-β1, transforming growth factor-β1.
Equilibria And Assessment Of Processes Between Air, Water, Sediments And Biota, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/between/equilibria+and+assessment+of+processes+between+air++water++sediments+and+biota/10__1590_slash_s0100___40422012000300026-206-8-11
Average 90 stars, based on 1 article reviews
equilibria and assessment of processes between air, water, sediments and biota - by Bioz Stars, 2026-09
90/100 stars
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cyclic peptides with a disulfide bridge between the cysteine residues  (GL Biochem)
90
GL Biochem cyclic peptides with a disulfide bridge between the cysteine residues
ISOF inhibited the expression of LUBAC in MLGs. (A) The protein levels <t>of</t> <t>SHARPIN</t> in the MLGs stimulated with TGF-β1 was analyzed by western blot. The western blot representative strip was listed on the right of figure. (B) The protein levels of HOIL-1L in the MLGs stimulated with TGF-β1 was analyzed by western blot. The western blot representative strip was listed on the right of figure. (C) The protein levels of <t>HOIP</t> in the MLGs stimulated with TGF-β1 was analyzed by western blot. The western blot representative strip was listed on the right of figure. ANOVA was used for western blotting statistics (SHARPIN, n=8; HOIL-1L, n=5; HOIP, n=10). ANOVA, analysis of variance; DXM, dexamethasone; GAPDH, Glyceraldehyde-3-phosphate dehydrogenase; HOIL-1L, heme oxidation IRP2 ubiquitin ligase 1L; HOIP, HOIL-1L interacting protein; HOIPIN-8, HOIP inhibitor-8; ISOF, isoforskolin; LUBAC, linear ubiquitin chain assembly complex; MLGs, mice lung fibroblasts; PFD, pirfenidone; SHARPIN, shank-associated RH domain interaction protein; TGF-β1, transforming growth factor-β1.
Cyclic Peptides With A Disulfide Bridge Between The Cysteine Residues, supplied by GL Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
cyclic peptides with a disulfide bridge between the cysteine residues - by Bioz Stars, 2026-09
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Image Search Results


ISOF inhibited the expression of LUBAC in MLGs. (A) The protein levels of SHARPIN in the MLGs stimulated with TGF-β1 was analyzed by western blot. The western blot representative strip was listed on the right of figure. (B) The protein levels of HOIL-1L in the MLGs stimulated with TGF-β1 was analyzed by western blot. The western blot representative strip was listed on the right of figure. (C) The protein levels of HOIP in the MLGs stimulated with TGF-β1 was analyzed by western blot. The western blot representative strip was listed on the right of figure. ANOVA was used for western blotting statistics (SHARPIN, n=8; HOIL-1L, n=5; HOIP, n=10). ANOVA, analysis of variance; DXM, dexamethasone; GAPDH, Glyceraldehyde-3-phosphate dehydrogenase; HOIL-1L, heme oxidation IRP2 ubiquitin ligase 1L; HOIP, HOIL-1L interacting protein; HOIPIN-8, HOIP inhibitor-8; ISOF, isoforskolin; LUBAC, linear ubiquitin chain assembly complex; MLGs, mice lung fibroblasts; PFD, pirfenidone; SHARPIN, shank-associated RH domain interaction protein; TGF-β1, transforming growth factor-β1.

Journal: Journal of Thoracic Disease

Article Title: Isoforskolin inhibits LUBAC/GSDMD/IL-1β cascades in pulmonary fibrosis

doi: 10.21037/jtd-2025-1950

Figure Lengend Snippet: ISOF inhibited the expression of LUBAC in MLGs. (A) The protein levels of SHARPIN in the MLGs stimulated with TGF-β1 was analyzed by western blot. The western blot representative strip was listed on the right of figure. (B) The protein levels of HOIL-1L in the MLGs stimulated with TGF-β1 was analyzed by western blot. The western blot representative strip was listed on the right of figure. (C) The protein levels of HOIP in the MLGs stimulated with TGF-β1 was analyzed by western blot. The western blot representative strip was listed on the right of figure. ANOVA was used for western blotting statistics (SHARPIN, n=8; HOIL-1L, n=5; HOIP, n=10). ANOVA, analysis of variance; DXM, dexamethasone; GAPDH, Glyceraldehyde-3-phosphate dehydrogenase; HOIL-1L, heme oxidation IRP2 ubiquitin ligase 1L; HOIP, HOIL-1L interacting protein; HOIPIN-8, HOIP inhibitor-8; ISOF, isoforskolin; LUBAC, linear ubiquitin chain assembly complex; MLGs, mice lung fibroblasts; PFD, pirfenidone; SHARPIN, shank-associated RH domain interaction protein; TGF-β1, transforming growth factor-β1.

Article Snippet: Anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (D16H11, XP ® Rabbit mAb#5174), IL-1β (D6D6T, Rabbit mAb#31202), GSDMD (E9S1X, Rabbit mAb#39754), beta-Actin (13E5, Rabbit mAb#4970) antibodies were derived from Cell Signaling Technology (Danvers, MA, USA), antibodies against HOIP (#A04457-3), SHARPIN (#A06687-1), and beta-Tubulin (#A05397-1) were purchased from Boster Biological (Wuhan, China).

Techniques: Expressing, Western Blot, Stripping Membranes, Ubiquitin Proteomics

ISOF reduced the expression level of LUBAC in IPF model mice. (A) The protein levels of SHARPIN in the lung tissues of each group was assessed by western blot. (B) The protein levels of HOIL-1L in the lung tissues of each group was assessed by western blot. (C) The protein levels of HOIP in the lung tissues of each group was assessed by western blot. The relative density of LUBAC subunits to GAPDH was calculated in each group. Data were expressed as means ± SEM (SHARPIN, n=7; HOIL-1L, n=8; HOIP, n=7). BLM, bleomycin; DXM, dexamethasone; GAPDH, Glyceraldehyde-3-phosphate dehydrogenase; HOIL-1L, heme oxidation IRP2 ubiquitin ligase 1L; HOIP, HOIL-1L interacting protein; IPF, idiopathic pulmonary fibrosis; ISOF, isoforskolin; LUBAC, linear ubiquitin chain assembly complex; PFD, pirfenidone; SEM, standard error of measurement; SHARPIN, shank-associated RH domain interaction protein.

Journal: Journal of Thoracic Disease

Article Title: Isoforskolin inhibits LUBAC/GSDMD/IL-1β cascades in pulmonary fibrosis

doi: 10.21037/jtd-2025-1950

Figure Lengend Snippet: ISOF reduced the expression level of LUBAC in IPF model mice. (A) The protein levels of SHARPIN in the lung tissues of each group was assessed by western blot. (B) The protein levels of HOIL-1L in the lung tissues of each group was assessed by western blot. (C) The protein levels of HOIP in the lung tissues of each group was assessed by western blot. The relative density of LUBAC subunits to GAPDH was calculated in each group. Data were expressed as means ± SEM (SHARPIN, n=7; HOIL-1L, n=8; HOIP, n=7). BLM, bleomycin; DXM, dexamethasone; GAPDH, Glyceraldehyde-3-phosphate dehydrogenase; HOIL-1L, heme oxidation IRP2 ubiquitin ligase 1L; HOIP, HOIL-1L interacting protein; IPF, idiopathic pulmonary fibrosis; ISOF, isoforskolin; LUBAC, linear ubiquitin chain assembly complex; PFD, pirfenidone; SEM, standard error of measurement; SHARPIN, shank-associated RH domain interaction protein.

Article Snippet: Anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (D16H11, XP ® Rabbit mAb#5174), IL-1β (D6D6T, Rabbit mAb#31202), GSDMD (E9S1X, Rabbit mAb#39754), beta-Actin (13E5, Rabbit mAb#4970) antibodies were derived from Cell Signaling Technology (Danvers, MA, USA), antibodies against HOIP (#A04457-3), SHARPIN (#A06687-1), and beta-Tubulin (#A05397-1) were purchased from Boster Biological (Wuhan, China).

Techniques: Expressing, Western Blot, Ubiquitin Proteomics