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Figure 5. Glomerular immune complex and C3c deposits in MRLlpr/lpr mice. Paraffin-embedded renal sections were stained for <t>IgG1</t> and <t>IgG2a</t> as indicated. In saline-treated mice, IgG1 and IgG2a localize to the mesangium and the glomeru- lar capillary wall. Note that IRS 661– and IRS 954–treated MRLlpr/lpr mice show less IgG2a deposits. Frozen renal sec- tions were stained for complement factor C3c. Note the robust glomerular complement activation in saline-treated MRLlpr/lpr mice, which is hardly detectable in IRS 661– and IRS 954–treated MRLlpr/lpr mice. Images are representative of 10 mice in each group. Magnification, 400.
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Figure 5. Glomerular immune complex and C3c deposits in MRLlpr/lpr mice. Paraffin-embedded renal sections were stained for IgG1 and IgG2a as indicated. In saline-treated mice, IgG1 and IgG2a localize to the mesangium and the glomeru- lar capillary wall. Note that IRS 661– and IRS 954–treated MRLlpr/lpr mice show less IgG2a deposits. Frozen renal sec- tions were stained for complement factor C3c. Note the robust glomerular complement activation in saline-treated MRLlpr/lpr mice, which is hardly detectable in IRS 661– and IRS 954–treated MRLlpr/lpr mice. Images are representative of 10 mice in each group. Magnification, 400.

Journal: Journal of the American Society of Nephrology

Article Title: Inhibition of Toll-Like Receptor-7 (TLR-7) or TLR-7 plus TLR-9 Attenuates Glomerulonephritis and Lung Injury in Experimental Lupus

doi: 10.1681/asn.2006101162

Figure Lengend Snippet: Figure 5. Glomerular immune complex and C3c deposits in MRLlpr/lpr mice. Paraffin-embedded renal sections were stained for IgG1 and IgG2a as indicated. In saline-treated mice, IgG1 and IgG2a localize to the mesangium and the glomeru- lar capillary wall. Note that IRS 661– and IRS 954–treated MRLlpr/lpr mice show less IgG2a deposits. Frozen renal sec- tions were stained for complement factor C3c. Note the robust glomerular complement activation in saline-treated MRLlpr/lpr mice, which is hardly detectable in IRS 661– and IRS 954–treated MRLlpr/lpr mice. Images are representative of 10 mice in each group. Magnification, 400.

Article Snippet: After incubation with mouse serum, dsDNA-specific IgG, IgG1, IgG2a, IgG2b, and IgG3 were detected by ELISA (Bethyl Labs, Montgomery, TX).

Techniques: Staining, Saline, Activation Assay

Figure 6. Localization of IRS 661 and IRS 954 in kidneys of MRLlpr/lpr mice after intraperitoneal injection. Rhodamine-la- beled IRS 661 and IRS 954 were intraperitoneally injected into 16-wk-old MRLlpr/lpr mice, and renal tissue was harvested 2 h later. (A) Fluorescence imaging of frozen sections showed up- take of labeled IRS 661 in glomeruli (encircled) in a mesangial and capillary staining pattern as well as in tubular epithelial cells. Co-staining with IgG showed partial co-localization of IRS 661 with glomerular IgG deposits. (B) At higher magnification, granular deposits of IRS 661 in tubular epithelial cells (left) and glomerular cells (right) can be seen. (C) Co-staining with a FITC-labeled Mac-2 antibody identified IRS 661 to co-localize with renal macrophages (arrow), whereas other renal macro- phages remain IRS 661 negative. Magnification, 400 in A and C.

Journal: Journal of the American Society of Nephrology

Article Title: Inhibition of Toll-Like Receptor-7 (TLR-7) or TLR-7 plus TLR-9 Attenuates Glomerulonephritis and Lung Injury in Experimental Lupus

doi: 10.1681/asn.2006101162

Figure Lengend Snippet: Figure 6. Localization of IRS 661 and IRS 954 in kidneys of MRLlpr/lpr mice after intraperitoneal injection. Rhodamine-la- beled IRS 661 and IRS 954 were intraperitoneally injected into 16-wk-old MRLlpr/lpr mice, and renal tissue was harvested 2 h later. (A) Fluorescence imaging of frozen sections showed up- take of labeled IRS 661 in glomeruli (encircled) in a mesangial and capillary staining pattern as well as in tubular epithelial cells. Co-staining with IgG showed partial co-localization of IRS 661 with glomerular IgG deposits. (B) At higher magnification, granular deposits of IRS 661 in tubular epithelial cells (left) and glomerular cells (right) can be seen. (C) Co-staining with a FITC-labeled Mac-2 antibody identified IRS 661 to co-localize with renal macrophages (arrow), whereas other renal macro- phages remain IRS 661 negative. Magnification, 400 in A and C.

Article Snippet: After incubation with mouse serum, dsDNA-specific IgG, IgG1, IgG2a, IgG2b, and IgG3 were detected by ELISA (Bethyl Labs, Montgomery, TX).

Techniques: Injection, Fluorescence, Imaging, Labeling, Staining