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Image Search Results
Journal: Journal of Traditional Chinese Medical Sciences
Article Title: Yiqi Huoxue Decoction modifies the expression of myocardial cytoskeleton-associated proteins by regulating the AMPK signaling pathway in H9c2 cells exposed to hypoxic conditions
doi: 10.1016/j.jtcms.2019.04.005
Figure Lengend Snippet: Figure 4 The effects of YQHX on the two isomers that make up microtubules in H9c2 cells subjected to hypoxic conditions for 12 hours. a-tubulin and b-tubulin expression were detected by western blot. Note: YQHX: Yiqi Huoxue Decoction. C: cells were cultured under normoxic condition; M: cells were placed in a hypoxic atmosphere filled with a gas mixture of 94% N2, 5% CO2, and 1% O2 for 12 hours; Y: YQHX was dissolved in Dulbecco’s Modified Eagle’s Medium at concentrations of 100 (Y1), 200 (Y2), or 400 (Y4) mg/mL, and cells were treated under hypoxic conditions for 12 hours. The values are expressed as the mean (standard deviation) of three independent experiments. * indicates P < .05, ** indicates P < .01, compared with the C group, # indicates P < .05; ## indicates P < .01 compared with the M group.
Article Snippet: The membrane was then blocked in TBS-T containing 5% skimmed milk and incubated with monoclonal antibodies against a-tubulin (11224-1-AP, Proteintech, Chicago, IL),
Techniques: Expressing, Western Blot, Cell Culture, Standard Deviation