Journal: Frontiers in Genetics

Article Title: A novel pathogenic APC variant identified in a Chinese pedigree with familial adenomatous polyposis

doi: 10.3389/fgene.2026.1776361

Figure Lengend Snippet: Effect of wild-type and mutant APC on β-catenin protein expression in SW480 cells. (A) Representative Western blot images showing β-catenin protein levels in the APC-WT group, APC-Mutant (c.3799 dup) group, and empty vector (Mock) group. β-actin was used as the internal reference to normalize protein loading. (B) Quantitative analysis of β-catenin protein expression. Data are presented as the mean ± standard error of the mean (SEM) from three independent biological replicates. Statistical significance was determined by one-way analysis of variance followed by Tukey’s multiple comparison test. *P < 0.05, P < 0.01 vs. the APC-WT group; ns: no significant difference between the empty vector group and APC-Mutant group.

Article Snippet: Equal protein (30 μg/lane) was separated by 10% SDS-PAGE, transferred to PVDF membranes, blocked with 5% non-fat milk (TBST, 1 h RT), and incubated with primary antibodies against β-catenin (1:1000 dilution, CST, #8480) and β-actin (1:20000 dilution, Proteintech, 66009-1-Ig) at 4 °C overnight.

Techniques: Mutagenesis, Expressing, Western Blot, Plasmid Preparation, Comparison