bestatin Search Results


915 100  (Gold Biotechnology Inc)
94
Gold Biotechnology Inc 915 100
915 100, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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bestatin  (R&D Systems)
94
R&D Systems bestatin
Bestatin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
bestatin - by Bioz Stars, 2026-02
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bestatin  (Valiant Co Ltd)
93
Valiant Co Ltd bestatin
Bestatin, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
bestatin - by Bioz Stars, 2026-02
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lda  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology lda
Lda, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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bestatin  (Selleck Chemicals)
93
Selleck Chemicals bestatin
Bestatin, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
bestatin - by Bioz Stars, 2026-02
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bestatin  (Tocris)
93
Tocris bestatin
Bestatin, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
bestatin - by Bioz Stars, 2026-02
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apn inhibitors bestatin  (TargetMol)
86
TargetMol apn inhibitors bestatin
a Cytotoxicity detection of 2,2′-dipyridyl, 1,10-phenanthroline, and <t>bestatin</t> by MTT assay. IPI-2I cells cultured in the 96-well plates were incubated with 2,2′-dipyridyl (250 μM), 1,10-phenanthroline (15 μM), or bestatin (300 μM). At 24 h after incubation, the inhibitors were removed, and MTT reagents (20 μL, 5 mg/mL) were added. After another 4 h incubation, the medium was discarded, and 150 μL of dimethyl sulfoxide (DMSO) solution was added. The OD value at 570 nm was measured. b The expression levels of <t>APN</t> in cells after treatment with the three inhibitors. IPI-2I cells were cultured in six-well plates and treated with 2,2′-dipyridyl (250 μM), 1,10-phenanthroline (15 μM), or bestatin (300 μM). At 24 h after treatment, the expression of endogenous pAPN was detected by western blot with anti-APN rabbit polyclonal antibody. c 2,2′-Dipyridyl (250 μM), 1,10-phenanthroline (15 μM), or bestatin (300 μM) was added to IPI-2I cells for 1 h. Cells were then infected with PDCoV (MOI = 2). At 24 h post infection, cells were analyzed by IFA. Mouse monoclonal antibody against PDCoV S was used to detect PDCoV-infected cells (green). DAPI was applied to detect nuclei (blue). d The fluorescence intensity in c was quantified with ImageJ. e , f IPI-2I cells were treated with the three inhibitors as described in c and infected with PDCoV (MOI = 2 in e or MOI = 0.2 in f ). At 24 h post infection, cells were collected, and the TCID 50 was determined in LLC-PK1 cells. Data are expressed as the mean ± SD for triplicate samples. Statistical significance was determined by Student’s t test; ns, P > 0.05; * P < 0.05; ** P < 0.01
Apn Inhibitors Bestatin, supplied by TargetMol, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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bestatin  (MedChemExpress)
94
MedChemExpress bestatin
a Cytotoxicity detection of 2,2′-dipyridyl, 1,10-phenanthroline, and <t>bestatin</t> by MTT assay. IPI-2I cells cultured in the 96-well plates were incubated with 2,2′-dipyridyl (250 μM), 1,10-phenanthroline (15 μM), or bestatin (300 μM). At 24 h after incubation, the inhibitors were removed, and MTT reagents (20 μL, 5 mg/mL) were added. After another 4 h incubation, the medium was discarded, and 150 μL of dimethyl sulfoxide (DMSO) solution was added. The OD value at 570 nm was measured. b The expression levels of <t>APN</t> in cells after treatment with the three inhibitors. IPI-2I cells were cultured in six-well plates and treated with 2,2′-dipyridyl (250 μM), 1,10-phenanthroline (15 μM), or bestatin (300 μM). At 24 h after treatment, the expression of endogenous pAPN was detected by western blot with anti-APN rabbit polyclonal antibody. c 2,2′-Dipyridyl (250 μM), 1,10-phenanthroline (15 μM), or bestatin (300 μM) was added to IPI-2I cells for 1 h. Cells were then infected with PDCoV (MOI = 2). At 24 h post infection, cells were analyzed by IFA. Mouse monoclonal antibody against PDCoV S was used to detect PDCoV-infected cells (green). DAPI was applied to detect nuclei (blue). d The fluorescence intensity in c was quantified with ImageJ. e , f IPI-2I cells were treated with the three inhibitors as described in c and infected with PDCoV (MOI = 2 in e or MOI = 0.2 in f ). At 24 h post infection, cells were collected, and the TCID 50 was determined in LLC-PK1 cells. Data are expressed as the mean ± SD for triplicate samples. Statistical significance was determined by Student’s t test; ns, P > 0.05; * P < 0.05; ** P < 0.01
Bestatin, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bestatin/product/MedChemExpress
Average 94 stars, based on 1 article reviews
bestatin - by Bioz Stars, 2026-02
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bestatin  (BOC Sciences)
91
BOC Sciences bestatin
a Cytotoxicity detection of 2,2′-dipyridyl, 1,10-phenanthroline, and <t>bestatin</t> by MTT assay. IPI-2I cells cultured in the 96-well plates were incubated with 2,2′-dipyridyl (250 μM), 1,10-phenanthroline (15 μM), or bestatin (300 μM). At 24 h after incubation, the inhibitors were removed, and MTT reagents (20 μL, 5 mg/mL) were added. After another 4 h incubation, the medium was discarded, and 150 μL of dimethyl sulfoxide (DMSO) solution was added. The OD value at 570 nm was measured. b The expression levels of <t>APN</t> in cells after treatment with the three inhibitors. IPI-2I cells were cultured in six-well plates and treated with 2,2′-dipyridyl (250 μM), 1,10-phenanthroline (15 μM), or bestatin (300 μM). At 24 h after treatment, the expression of endogenous pAPN was detected by western blot with anti-APN rabbit polyclonal antibody. c 2,2′-Dipyridyl (250 μM), 1,10-phenanthroline (15 μM), or bestatin (300 μM) was added to IPI-2I cells for 1 h. Cells were then infected with PDCoV (MOI = 2). At 24 h post infection, cells were analyzed by IFA. Mouse monoclonal antibody against PDCoV S was used to detect PDCoV-infected cells (green). DAPI was applied to detect nuclei (blue). d The fluorescence intensity in c was quantified with ImageJ. e , f IPI-2I cells were treated with the three inhibitors as described in c and infected with PDCoV (MOI = 2 in e or MOI = 0.2 in f ). At 24 h post infection, cells were collected, and the TCID 50 was determined in LLC-PK1 cells. Data are expressed as the mean ± SD for triplicate samples. Statistical significance was determined by Student’s t test; ns, P > 0.05; * P < 0.05; ** P < 0.01
Bestatin, supplied by BOC Sciences, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 1 article reviews
bestatin - by Bioz Stars, 2026-02
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ubenimex (bestatin, mw: 308.4, a substance produced by streptomyces olivoreticuli)  (FUJIFILM)
90
FUJIFILM ubenimex (bestatin, mw: 308.4, a substance produced by streptomyces olivoreticuli)
a Cytotoxicity detection of 2,2′-dipyridyl, 1,10-phenanthroline, and <t>bestatin</t> by MTT assay. IPI-2I cells cultured in the 96-well plates were incubated with 2,2′-dipyridyl (250 μM), 1,10-phenanthroline (15 μM), or bestatin (300 μM). At 24 h after incubation, the inhibitors were removed, and MTT reagents (20 μL, 5 mg/mL) were added. After another 4 h incubation, the medium was discarded, and 150 μL of dimethyl sulfoxide (DMSO) solution was added. The OD value at 570 nm was measured. b The expression levels of <t>APN</t> in cells after treatment with the three inhibitors. IPI-2I cells were cultured in six-well plates and treated with 2,2′-dipyridyl (250 μM), 1,10-phenanthroline (15 μM), or bestatin (300 μM). At 24 h after treatment, the expression of endogenous pAPN was detected by western blot with anti-APN rabbit polyclonal antibody. c 2,2′-Dipyridyl (250 μM), 1,10-phenanthroline (15 μM), or bestatin (300 μM) was added to IPI-2I cells for 1 h. Cells were then infected with PDCoV (MOI = 2). At 24 h post infection, cells were analyzed by IFA. Mouse monoclonal antibody against PDCoV S was used to detect PDCoV-infected cells (green). DAPI was applied to detect nuclei (blue). d The fluorescence intensity in c was quantified with ImageJ. e , f IPI-2I cells were treated with the three inhibitors as described in c and infected with PDCoV (MOI = 2 in e or MOI = 0.2 in f ). At 24 h post infection, cells were collected, and the TCID 50 was determined in LLC-PK1 cells. Data are expressed as the mean ± SD for triplicate samples. Statistical significance was determined by Student’s t test; ns, P > 0.05; * P < 0.05; ** P < 0.01
Ubenimex (Bestatin, Mw: 308.4, A Substance Produced By Streptomyces Olivoreticuli), supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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proteinase inhibitor mixture contains aebsf, aprotinin, bestatin, e-64, leupeptin pepstatin  (FUJIFILM)
90
FUJIFILM proteinase inhibitor mixture contains aebsf, aprotinin, bestatin, e-64, leupeptin pepstatin
a Cytotoxicity detection of 2,2′-dipyridyl, 1,10-phenanthroline, and <t>bestatin</t> by MTT assay. IPI-2I cells cultured in the 96-well plates were incubated with 2,2′-dipyridyl (250 μM), 1,10-phenanthroline (15 μM), or bestatin (300 μM). At 24 h after incubation, the inhibitors were removed, and MTT reagents (20 μL, 5 mg/mL) were added. After another 4 h incubation, the medium was discarded, and 150 μL of dimethyl sulfoxide (DMSO) solution was added. The OD value at 570 nm was measured. b The expression levels of <t>APN</t> in cells after treatment with the three inhibitors. IPI-2I cells were cultured in six-well plates and treated with 2,2′-dipyridyl (250 μM), 1,10-phenanthroline (15 μM), or bestatin (300 μM). At 24 h after treatment, the expression of endogenous pAPN was detected by western blot with anti-APN rabbit polyclonal antibody. c 2,2′-Dipyridyl (250 μM), 1,10-phenanthroline (15 μM), or bestatin (300 μM) was added to IPI-2I cells for 1 h. Cells were then infected with PDCoV (MOI = 2). At 24 h post infection, cells were analyzed by IFA. Mouse monoclonal antibody against PDCoV S was used to detect PDCoV-infected cells (green). DAPI was applied to detect nuclei (blue). d The fluorescence intensity in c was quantified with ImageJ. e , f IPI-2I cells were treated with the three inhibitors as described in c and infected with PDCoV (MOI = 2 in e or MOI = 0.2 in f ). At 24 h post infection, cells were collected, and the TCID 50 was determined in LLC-PK1 cells. Data are expressed as the mean ± SD for triplicate samples. Statistical significance was determined by Student’s t test; ns, P > 0.05; * P < 0.05; ** P < 0.01
Proteinase Inhibitor Mixture Contains Aebsf, Aprotinin, Bestatin, E 64, Leupeptin Pepstatin, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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proteinase inhibitor mixture contains aebsf, aprotinin, bestatin, e-64, leupeptin pepstatin - by Bioz Stars, 2026-02
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Image Search Results


a Cytotoxicity detection of 2,2′-dipyridyl, 1,10-phenanthroline, and bestatin by MTT assay. IPI-2I cells cultured in the 96-well plates were incubated with 2,2′-dipyridyl (250 μM), 1,10-phenanthroline (15 μM), or bestatin (300 μM). At 24 h after incubation, the inhibitors were removed, and MTT reagents (20 μL, 5 mg/mL) were added. After another 4 h incubation, the medium was discarded, and 150 μL of dimethyl sulfoxide (DMSO) solution was added. The OD value at 570 nm was measured. b The expression levels of APN in cells after treatment with the three inhibitors. IPI-2I cells were cultured in six-well plates and treated with 2,2′-dipyridyl (250 μM), 1,10-phenanthroline (15 μM), or bestatin (300 μM). At 24 h after treatment, the expression of endogenous pAPN was detected by western blot with anti-APN rabbit polyclonal antibody. c 2,2′-Dipyridyl (250 μM), 1,10-phenanthroline (15 μM), or bestatin (300 μM) was added to IPI-2I cells for 1 h. Cells were then infected with PDCoV (MOI = 2). At 24 h post infection, cells were analyzed by IFA. Mouse monoclonal antibody against PDCoV S was used to detect PDCoV-infected cells (green). DAPI was applied to detect nuclei (blue). d The fluorescence intensity in c was quantified with ImageJ. e , f IPI-2I cells were treated with the three inhibitors as described in c and infected with PDCoV (MOI = 2 in e or MOI = 0.2 in f ). At 24 h post infection, cells were collected, and the TCID 50 was determined in LLC-PK1 cells. Data are expressed as the mean ± SD for triplicate samples. Statistical significance was determined by Student’s t test; ns, P > 0.05; * P < 0.05; ** P < 0.01

Journal: Emerging Microbes & Infections

Article Title: Contribution of porcine aminopeptidase N to porcine deltacoronavirus infection

doi: 10.1038/s41426-018-0068-3

Figure Lengend Snippet: a Cytotoxicity detection of 2,2′-dipyridyl, 1,10-phenanthroline, and bestatin by MTT assay. IPI-2I cells cultured in the 96-well plates were incubated with 2,2′-dipyridyl (250 μM), 1,10-phenanthroline (15 μM), or bestatin (300 μM). At 24 h after incubation, the inhibitors were removed, and MTT reagents (20 μL, 5 mg/mL) were added. After another 4 h incubation, the medium was discarded, and 150 μL of dimethyl sulfoxide (DMSO) solution was added. The OD value at 570 nm was measured. b The expression levels of APN in cells after treatment with the three inhibitors. IPI-2I cells were cultured in six-well plates and treated with 2,2′-dipyridyl (250 μM), 1,10-phenanthroline (15 μM), or bestatin (300 μM). At 24 h after treatment, the expression of endogenous pAPN was detected by western blot with anti-APN rabbit polyclonal antibody. c 2,2′-Dipyridyl (250 μM), 1,10-phenanthroline (15 μM), or bestatin (300 μM) was added to IPI-2I cells for 1 h. Cells were then infected with PDCoV (MOI = 2). At 24 h post infection, cells were analyzed by IFA. Mouse monoclonal antibody against PDCoV S was used to detect PDCoV-infected cells (green). DAPI was applied to detect nuclei (blue). d The fluorescence intensity in c was quantified with ImageJ. e , f IPI-2I cells were treated with the three inhibitors as described in c and infected with PDCoV (MOI = 2 in e or MOI = 0.2 in f ). At 24 h post infection, cells were collected, and the TCID 50 was determined in LLC-PK1 cells. Data are expressed as the mean ± SD for triplicate samples. Statistical significance was determined by Student’s t test; ns, P > 0.05; * P < 0.05; ** P < 0.01

Article Snippet: APN inhibitors bestatin (TargetMol, USA), 2,2′-dipyridyl, and 1,10-phenanthroline (Sigma, USA) were each dissolved in water at a concentration of 200 mM.

Techniques: MTT Assay, Cell Culture, Incubation, Expressing, Western Blot, Infection, Fluorescence