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Image Search Results
Journal: Cell Death & Disease
Article Title: Dual targeting of PI3Kδ and PPARα enhances antitumor activity via FoxO1 activation in follicular lymphoma
doi: 10.1038/s41419-026-08593-5
Figure Lengend Snippet: A – C Representative Annexin V/PI flow-cytometry plots in Karpas-422, RL, and Sc-1 cells after treatment. D – F Quantification of Annexin V–positive cells ( n = 3). G Gene Set Enrichment Analysis (GSEA) showing enrichment of apoptosis-related pathways (NES = 1.58837, p = 0.00047). H – J Western blots of apoptosis-related proteins (cleaved PARP, Bcl-2, Mcl-1, Bim, Bax) in Karpas-422, RL, and Sc-1 cells; combination treatment increased cleaved PARP, Bim, and Bax and reduced Mcl-1, while Bcl-2 showed no consistent change (representative of three independent experiments).
Article Snippet: The primary antibodies used were as follows: PI3K p110δ (A19742; ABclonal), pan-Akt (#4691; CST), phospho-Akt (Ser473, #4060; CST), PPARα (ab227074; Abcam), FoxO1 (#2880; CST), phospho-FoxO1 (Thr24, #9464; CST) and (Ser256, #9461; CST), FoxO3a (#12829; CST), FoxO4 (#9472; CST), Mcl-1 (#16225-1-AP; Proteintech),
Techniques: Flow Cytometry, Western Blot