bbsi Search Results


bbsi  (New England Biolabs)
99
New England Biolabs bbsi
Bbsi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bbsi/BbsI/10__3390_slash_ijms27052418-160-28-29
Average 99 stars, based on 1 article reviews
bbsi - by Bioz Stars, 2026-09
99/100 stars
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pklv2 u6grna5 bbsi pgkpuro2azsg w  (Addgene inc)
93
Addgene inc pklv2 u6grna5 bbsi pgkpuro2azsg w
Pklv2 U6grna5 Bbsi Pgkpuro2azsg W, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bbsi/pKLV2-U6gRNA5(BbsI)-PGKpuro2AZsG-W+(Plasmid+%2367975)/pmc12061241-224-14-15
Average 93 stars, based on 1 article reviews
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93/100 stars
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bbsi digested pklv2u6grna5 pgkpuro2abfp  (Addgene inc)
93
Addgene inc bbsi digested pklv2u6grna5 pgkpuro2abfp
Bbsi Digested Pklv2u6grna5 Pgkpuro2abfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bbsi/pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP+(Plasmid+%2367991)/pm41328592-80-33-35
Average 93 stars, based on 1 article reviews
bbsi digested pklv2u6grna5 pgkpuro2abfp - by Bioz Stars, 2026-09
93/100 stars
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workflow a pu6  (Addgene inc)
93
Addgene inc workflow a pu6
Workflow A Pu6, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bbsi/pU6-(BbsI)sgRNA_CAG-Cas9-bpA_EF1-TagRFP+(Plasmid+%2386987)/pm28522326-154-0-4
Average 93 stars, based on 1 article reviews
workflow a pu6 - by Bioz Stars, 2026-09
93/100 stars
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pklv2 u6grna5 bbsi pgkpuro2abfp w  (Addgene inc)
95
Addgene inc pklv2 u6grna5 bbsi pgkpuro2abfp w
Pklv2 U6grna5 Bbsi Pgkpuro2abfp W, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bbsi/pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W+(Plasmid+%2367974)/10__1158_slash_1535___7163__mct___21___0532-81-20-23
Average 95 stars, based on 1 article reviews
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95/100 stars
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pu6  (Addgene inc)
93
Addgene inc pu6
Schematic for <t>CRISPR-Cas9-mediated</t> knockin of exogenous DNA fragment into human AAVS1 locus in HEK293 cells Top: the human AAVS1 allele. gRNA sequences are designated (g1 and g2) and located upstream of exon 2. Center: donor plasmid is represented schematically. Dashed lines show homology arms (∼800 bp on each side of donor) between genomic locus and plasmid DNA. A splice acceptor sequence is inserted upstream of RFP gene to allow proper RNA splicing using the endogenous transcriptional promoter. <t>T2A</t> sequences are inserted both upstream and between RFP/AncUOX to allow the release of the individual proteins after translation. SV40 poly(A) signal sequence is designated downstream of the donor DNA. Bottom: engineered allele. Post Cas9 cuts at the site corresponding to the gRNA sequences, donor DNA is integrated into the breakpoint through homology directed repair (HDR).
Pu6, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bbsi/pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E4orf6+(Plasmid+%2364222)/pmc08463316-121-3-10
Average 93 stars, based on 1 article reviews
pu6 - by Bioz Stars, 2026-09
93/100 stars
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pkvl2 u6grna sam bbsi pgkpurobfp w plasmid  (Addgene inc)
93
Addgene inc pkvl2 u6grna sam bbsi pgkpurobfp w plasmid
Schematic for <t>CRISPR-Cas9-mediated</t> knockin of exogenous DNA fragment into human AAVS1 locus in HEK293 cells Top: the human AAVS1 allele. gRNA sequences are designated (g1 and g2) and located upstream of exon 2. Center: donor plasmid is represented schematically. Dashed lines show homology arms (∼800 bp on each side of donor) between genomic locus and plasmid DNA. A splice acceptor sequence is inserted upstream of RFP gene to allow proper RNA splicing using the endogenous transcriptional promoter. <t>T2A</t> sequences are inserted both upstream and between RFP/AncUOX to allow the release of the individual proteins after translation. SV40 poly(A) signal sequence is designated downstream of the donor DNA. Bottom: engineered allele. Post Cas9 cuts at the site corresponding to the gRNA sequences, donor DNA is integrated into the breakpoint through homology directed repair (HDR).
Pkvl2 U6grna Sam Bbsi Pgkpurobfp W Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bbsi/pKVL2-U6gRNA_SAM(BbsI)-PGKpuroBFP-W+(Plasmid+%23112925)/pm37858248-86-17-19
Average 93 stars, based on 1 article reviews
pkvl2 u6grna sam bbsi pgkpurobfp w plasmid - by Bioz Stars, 2026-09
93/100 stars
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px601 mogs ab  (Addgene inc)
93
Addgene inc px601 mogs ab
Schematic for <t>CRISPR-Cas9-mediated</t> knockin of exogenous DNA fragment into human AAVS1 locus in HEK293 cells Top: the human AAVS1 allele. gRNA sequences are designated (g1 and g2) and located upstream of exon 2. Center: donor plasmid is represented schematically. Dashed lines show homology arms (∼800 bp on each side of donor) between genomic locus and plasmid DNA. A splice acceptor sequence is inserted upstream of RFP gene to allow proper RNA splicing using the endogenous transcriptional promoter. <t>T2A</t> sequences are inserted both upstream and between RFP/AncUOX to allow the release of the individual proteins after translation. SV40 poly(A) signal sequence is designated downstream of the donor DNA. Bottom: engineered allele. Post Cas9 cuts at the site corresponding to the gRNA sequences, donor DNA is integrated into the breakpoint through homology directed repair (HDR).
Px601 Mogs Ab, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bbsi/pKLV-U6gRNA-EF(BbsI)-PGKpuro2ABFP+(Plasmid+%2362348)/pm37346975-150-5-8
Average 93 stars, based on 1 article reviews
px601 mogs ab - by Bioz Stars, 2026-09
93/100 stars
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puro2abfp w vectors  (Addgene inc)
93
Addgene inc puro2abfp w vectors
Schematic for <t>CRISPR-Cas9-mediated</t> knockin of exogenous DNA fragment into human AAVS1 locus in HEK293 cells Top: the human AAVS1 allele. gRNA sequences are designated (g1 and g2) and located upstream of exon 2. Center: donor plasmid is represented schematically. Dashed lines show homology arms (∼800 bp on each side of donor) between genomic locus and plasmid DNA. A splice acceptor sequence is inserted upstream of RFP gene to allow proper RNA splicing using the endogenous transcriptional promoter. <t>T2A</t> sequences are inserted both upstream and between RFP/AncUOX to allow the release of the individual proteins after translation. SV40 poly(A) signal sequence is designated downstream of the donor DNA. Bottom: engineered allele. Post Cas9 cuts at the site corresponding to the gRNA sequences, donor DNA is integrated into the breakpoint through homology directed repair (HDR).
Puro2abfp W Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bbsi/pKLV2-U6gRNA5(BbsI)-PGKpuro2AmCherry-W+(Plasmid+%2367977)/pmc11497402-30-18-24
Average 93 stars, based on 1 article reviews
puro2abfp w vectors - by Bioz Stars, 2026-09
93/100 stars
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pu6 bbsi cbh cas9 t2a mcherry  (Addgene inc)
96
Addgene inc pu6 bbsi cbh cas9 t2a mcherry
Schematic for <t>CRISPR-Cas9-mediated</t> knockin of exogenous DNA fragment into human AAVS1 locus in HEK293 cells Top: the human AAVS1 allele. gRNA sequences are designated (g1 and g2) and located upstream of exon 2. Center: donor plasmid is represented schematically. Dashed lines show homology arms (∼800 bp on each side of donor) between genomic locus and plasmid DNA. A splice acceptor sequence is inserted upstream of RFP gene to allow proper RNA splicing using the endogenous transcriptional promoter. <t>T2A</t> sequences are inserted both upstream and between RFP/AncUOX to allow the release of the individual proteins after translation. SV40 poly(A) signal sequence is designated downstream of the donor DNA. Bottom: engineered allele. Post Cas9 cuts at the site corresponding to the gRNA sequences, donor DNA is integrated into the breakpoint through homology directed repair (HDR).
Pu6 Bbsi Cbh Cas9 T2a Mcherry, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bbsi/pU6-(BbsI)_CBh-Cas9-T2A-mCherry+(Plasmid+%2364324)/pm38978356-123-41-43
Average 96 stars, based on 1 article reviews
pu6 bbsi cbh cas9 t2a mcherry - by Bioz Stars, 2026-09
96/100 stars
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plasmid px330 bbsi pitch  (Addgene inc)
93
Addgene inc plasmid px330 bbsi pitch
Schematic for <t>CRISPR-Cas9-mediated</t> knockin of exogenous DNA fragment into human AAVS1 locus in HEK293 cells Top: the human AAVS1 allele. gRNA sequences are designated (g1 and g2) and located upstream of exon 2. Center: donor plasmid is represented schematically. Dashed lines show homology arms (∼800 bp on each side of donor) between genomic locus and plasmid DNA. A splice acceptor sequence is inserted upstream of RFP gene to allow proper RNA splicing using the endogenous transcriptional promoter. <t>T2A</t> sequences are inserted both upstream and between RFP/AncUOX to allow the release of the individual proteins after translation. SV40 poly(A) signal sequence is designated downstream of the donor DNA. Bottom: engineered allele. Post Cas9 cuts at the site corresponding to the gRNA sequences, donor DNA is integrated into the breakpoint through homology directed repair (HDR).
Plasmid Px330 Bbsi Pitch, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bbsi/pX330-BbsI-PITCh+(Plasmid+%23127875)/pmc09475550-369-8-10
Average 93 stars, based on 1 article reviews
plasmid px330 bbsi pitch - by Bioz Stars, 2026-09
93/100 stars
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pklv2 2 mu6grna5 sapi hu6grna5 bbsi pgkpurobfp w  (Addgene inc)
93
Addgene inc pklv2 2 mu6grna5 sapi hu6grna5 bbsi pgkpurobfp w
Schematic for <t>CRISPR-Cas9-mediated</t> knockin of exogenous DNA fragment into human AAVS1 locus in HEK293 cells Top: the human AAVS1 allele. gRNA sequences are designated (g1 and g2) and located upstream of exon 2. Center: donor plasmid is represented schematically. Dashed lines show homology arms (∼800 bp on each side of donor) between genomic locus and plasmid DNA. A splice acceptor sequence is inserted upstream of RFP gene to allow proper RNA splicing using the endogenous transcriptional promoter. <t>T2A</t> sequences are inserted both upstream and between RFP/AncUOX to allow the release of the individual proteins after translation. SV40 poly(A) signal sequence is designated downstream of the donor DNA. Bottom: engineered allele. Post Cas9 cuts at the site corresponding to the gRNA sequences, donor DNA is integrated into the breakpoint through homology directed repair (HDR).
Pklv2 2 Mu6grna5 Sapi Hu6grna5 Bbsi Pgkpurobfp W, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bbsi/pKLV2%2E2-mU6gRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W+(Plasmid+%2372667)/pmc11999688-231-10-11
Average 93 stars, based on 1 article reviews
pklv2 2 mu6grna5 sapi hu6grna5 bbsi pgkpurobfp w - by Bioz Stars, 2026-09
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Image Search Results


Schematic for CRISPR-Cas9-mediated knockin of exogenous DNA fragment into human AAVS1 locus in HEK293 cells Top: the human AAVS1 allele. gRNA sequences are designated (g1 and g2) and located upstream of exon 2. Center: donor plasmid is represented schematically. Dashed lines show homology arms (∼800 bp on each side of donor) between genomic locus and plasmid DNA. A splice acceptor sequence is inserted upstream of RFP gene to allow proper RNA splicing using the endogenous transcriptional promoter. T2A sequences are inserted both upstream and between RFP/AncUOX to allow the release of the individual proteins after translation. SV40 poly(A) signal sequence is designated downstream of the donor DNA. Bottom: engineered allele. Post Cas9 cuts at the site corresponding to the gRNA sequences, donor DNA is integrated into the breakpoint through homology directed repair (HDR).

Journal: Molecular Therapy. Nucleic Acids

Article Title: CRISPR-Cas9-mediated reactivation of the uricase pseudogene in human cells prevents acute hyperuricemia

doi: 10.1016/j.omtn.2021.08.002

Figure Lengend Snippet: Schematic for CRISPR-Cas9-mediated knockin of exogenous DNA fragment into human AAVS1 locus in HEK293 cells Top: the human AAVS1 allele. gRNA sequences are designated (g1 and g2) and located upstream of exon 2. Center: donor plasmid is represented schematically. Dashed lines show homology arms (∼800 bp on each side of donor) between genomic locus and plasmid DNA. A splice acceptor sequence is inserted upstream of RFP gene to allow proper RNA splicing using the endogenous transcriptional promoter. T2A sequences are inserted both upstream and between RFP/AncUOX to allow the release of the individual proteins after translation. SV40 poly(A) signal sequence is designated downstream of the donor DNA. Bottom: engineered allele. Post Cas9 cuts at the site corresponding to the gRNA sequences, donor DNA is integrated into the breakpoint through homology directed repair (HDR).

Article Snippet: To construct the pU6-(Bbs1)_CBh-Cas9-T2A-GFP-p2A-Ad4E4orf6 plasmid (CRISPR plasmid), we digested px458 (Addgene, #48138) and pU6-(BbsI)_CBh-Cas9-T2A-mCherry-P2A-Ad4E4orf6 (Addgene, #64222) with FseI/BsrGI (New England Biolabs).

Techniques: CRISPR, Knock-In, Plasmid Preparation, Sequencing