Journal:

Article Title: The role of CD154-CD40 versus CD28-B7 costimulatory pathways in regulating allogeneic Th1 and Th2 responses in vivo

doi:

Figure Lengend Snippet: Allograft survival in untreated recipients. C57BL/6 hearts were transplanted into wild-type, STAT4–/–, or STAT6–/– BALB/c recipients.

Article Snippet: C57BL/6 (H-2 b ) and C3H/He (H-2 k ) mice aged 6–8 weeks were purchased from the Jackson Laboratory (Bar Harbor, Maine, USA) and BALB/c (H-2 d ) mice aged 6–8 weeks were purchased from Taconic Farms (Germantown, New York, USA).

Techniques:

Journal:

Article Title: The role of CD154-CD40 versus CD28-B7 costimulatory pathways in regulating allogeneic Th1 and Th2 responses in vivo

doi:

Figure Lengend Snippet: Frequency of cytokine-producing donor-specific T cells in recipient WT, STAT4–/–, and STAT6–/– BALB/c mice following allogeneic heart transplantation. Mice were transplanted with C57BL/6 allogeneic hearts. Recipient spleens were removed at the time of rejection (9–10 days after transplant). Splenocytes (1.2 × 106 cells per well) were incubated with either recipient (open bars) or donor (filled bars) irradiated splenocytes. The frequencies of IFN-γ–producing T cells (a) and IL-5–producing T cells (b) were then determined using ELISPOT assay as described in Methods. The results are expressed as the mean number of spots per well ± SE obtained from three to four mice tested individually in each group. P values of syngeneic versus alloreactive responder frequencies in each animal group are as follows. IFN-γ: P = 0.0196, 0.0132, and 0.006 in WT, STAT4–/–, and STAT6–/– recipients, respectively. IL-5: P = 0.2327, 0.0092, and 0.0942 in WT, STAT4–/–, and STAT6–/– recipients, respectively. AP = 0.002; BP = 0.0493.

Article Snippet: C57BL/6 (H-2 b ) and C3H/He (H-2 k ) mice aged 6–8 weeks were purchased from the Jackson Laboratory (Bar Harbor, Maine, USA) and BALB/c (H-2 d ) mice aged 6–8 weeks were purchased from Taconic Farms (Germantown, New York, USA).

Techniques: Transplantation Assay, Incubation, Irradiation, Enzyme-linked Immunospot

Journal:

Article Title: The role of CD154-CD40 versus CD28-B7 costimulatory pathways in regulating allogeneic Th1 and Th2 responses in vivo

doi:

Figure Lengend Snippet: Effect of administration of multiple injections of MR1 on cardiac allograft survival in WT and STAT6–/– recipients. C57BL/6 hearts were transplanted into wild-type or STAT6–/– BALB/c recipients. Recipients were treated with either a single dose of MR1 alone (250 μg intraperitoneally) on the day of transplantation or four doses of MR1 (250 μg intraperitoneally, four times) on post-transplant day 0, 2, 4, and 6. The multiple-injection protocol significantly increased graft survival in both recipient groups. AP = 0.0456; BP = 0.0043.

Article Snippet: C57BL/6 (H-2 b ) and C3H/He (H-2 k ) mice aged 6–8 weeks were purchased from the Jackson Laboratory (Bar Harbor, Maine, USA) and BALB/c (H-2 d ) mice aged 6–8 weeks were purchased from Taconic Farms (Germantown, New York, USA).

Techniques: Transplantation Assay, Injection

Journal:

Article Title: The role of CD154-CD40 versus CD28-B7 costimulatory pathways in regulating allogeneic Th1 and Th2 responses in vivo

doi:

Figure Lengend Snippet: Effect of coadministration of donor splenocytes plus a single injection of MR1 on cardiac allograft survival in WT, STAT4–/–, and STAT6–/– recipients. C57BL/6 hearts were transplanted into WT, STAT4–/–, or STAT6–/– BALB/c recipients. Recipients were treated with MR1 (250 μg intraperitoneally) plus 5 × 106 donor splenocytes intravenously on the day of transplantation. There were no significant differences between the three recipient types.

Article Snippet: C57BL/6 (H-2 b ) and C3H/He (H-2 k ) mice aged 6–8 weeks were purchased from the Jackson Laboratory (Bar Harbor, Maine, USA) and BALB/c (H-2 d ) mice aged 6–8 weeks were purchased from Taconic Farms (Germantown, New York, USA).

Techniques: Injection, Transplantation Assay