bafilomycin a1 bfa Search Results


bafilomycin a1 bfa  (MedChemExpress)
99
MedChemExpress bafilomycin a1 bfa
FIGURE 4 Areg deficiency upregulates autophagosome formation and inhibits autophagosome clearance. (A) LC3-II, p62, and GAPDH protein levels in the infarct border myocardium from WT and Areg−/− mice were quantified by western blotting on day 7 after MI. Left, representative immunoblot. Right, summary data. n = 4 per group. (B) WT and Areg−/− mice treated with 3-MA (30 μg/g/day) or vehicle were administered at the same time each day for 7 days after MI. LC3-II and GAPDH protein levels in the infarct border myocardium from WT and Areg−/− mice treated with 3-MA or vehicles were quantified by western blotting. Top, representative immunoblot. Bottom, summary data. n = 6 per group. (C) Seven days after MI, WT, and Areg−/− mice were injected with <t>BFA</t> (1.5 μg/g) or vehicles and sacrificed 2 h after for the assays. LC3-II and GAPDH protein levels in the infarct border myocardium from WT and Areg−/− mice treated with BFA or vehicles were quantified by western blotting. Top, representative immunoblot. Bottom, summary data. n = 5 per group. (D) Electron micrographs of the infarct border myocardium from WT (left) and Areg−/− (middle) hearts, were analyzed for the autophagic vacuole density (right). n = 4–5 per group. Scale bar, 1 μm. Data are expressed as means ± SEM. *p < .05, **p < .01. Data in (A) were analyzed by a two-tailed unpaired t-test. One-way ANOVA with the Tukey post hoc test was performed in (B) and (C). Data in (D) were analyzed with the Mann–Whitney test. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; i.p., intraperitoneal injection; 3-MA, 3-methyladenine; BFA, <t>bafilomycin-A1.</t>
Bafilomycin A1 Bfa, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bafilomycin+a1+bfa/Bafilomycin+A1/pm38358359-42-0-6
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bafilomycin 1 (bfa  (LC Laboratories)
90
LC Laboratories bafilomycin 1 (bfa
FIGURE 4 Areg deficiency upregulates autophagosome formation and inhibits autophagosome clearance. (A) LC3-II, p62, and GAPDH protein levels in the infarct border myocardium from WT and Areg−/− mice were quantified by western blotting on day 7 after MI. Left, representative immunoblot. Right, summary data. n = 4 per group. (B) WT and Areg−/− mice treated with 3-MA (30 μg/g/day) or vehicle were administered at the same time each day for 7 days after MI. LC3-II and GAPDH protein levels in the infarct border myocardium from WT and Areg−/− mice treated with 3-MA or vehicles were quantified by western blotting. Top, representative immunoblot. Bottom, summary data. n = 6 per group. (C) Seven days after MI, WT, and Areg−/− mice were injected with <t>BFA</t> (1.5 μg/g) or vehicles and sacrificed 2 h after for the assays. LC3-II and GAPDH protein levels in the infarct border myocardium from WT and Areg−/− mice treated with BFA or vehicles were quantified by western blotting. Top, representative immunoblot. Bottom, summary data. n = 5 per group. (D) Electron micrographs of the infarct border myocardium from WT (left) and Areg−/− (middle) hearts, were analyzed for the autophagic vacuole density (right). n = 4–5 per group. Scale bar, 1 μm. Data are expressed as means ± SEM. *p < .05, **p < .01. Data in (A) were analyzed by a two-tailed unpaired t-test. One-way ANOVA with the Tukey post hoc test was performed in (B) and (C). Data in (D) were analyzed with the Mann–Whitney test. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; i.p., intraperitoneal injection; 3-MA, 3-methyladenine; BFA, <t>bafilomycin-A1.</t>
Bafilomycin 1 (Bfa, supplied by LC Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bafilomycin+a1+bfa/bafilomycin+a1+b+1080/pmc03121344-186-2-9
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bafilomycin a1 bfa  (Selleck Chemicals)
96
Selleck Chemicals bafilomycin a1 bfa
FIGURE 4 Areg deficiency upregulates autophagosome formation and inhibits autophagosome clearance. (A) LC3-II, p62, and GAPDH protein levels in the infarct border myocardium from WT and Areg−/− mice were quantified by western blotting on day 7 after MI. Left, representative immunoblot. Right, summary data. n = 4 per group. (B) WT and Areg−/− mice treated with 3-MA (30 μg/g/day) or vehicle were administered at the same time each day for 7 days after MI. LC3-II and GAPDH protein levels in the infarct border myocardium from WT and Areg−/− mice treated with 3-MA or vehicles were quantified by western blotting. Top, representative immunoblot. Bottom, summary data. n = 6 per group. (C) Seven days after MI, WT, and Areg−/− mice were injected with <t>BFA</t> (1.5 μg/g) or vehicles and sacrificed 2 h after for the assays. LC3-II and GAPDH protein levels in the infarct border myocardium from WT and Areg−/− mice treated with BFA or vehicles were quantified by western blotting. Top, representative immunoblot. Bottom, summary data. n = 5 per group. (D) Electron micrographs of the infarct border myocardium from WT (left) and Areg−/− (middle) hearts, were analyzed for the autophagic vacuole density (right). n = 4–5 per group. Scale bar, 1 μm. Data are expressed as means ± SEM. *p < .05, **p < .01. Data in (A) were analyzed by a two-tailed unpaired t-test. One-way ANOVA with the Tukey post hoc test was performed in (B) and (C). Data in (D) were analyzed with the Mann–Whitney test. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; i.p., intraperitoneal injection; 3-MA, 3-methyladenine; BFA, <t>bafilomycin-A1.</t>
Bafilomycin A1 Bfa, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bafilomycin+a1+bfa/Bafilomycin+A1/pmc06452544-49-0-6
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stock bafilomycin a1  (Santa Cruz Biotechnology)
95
Santa Cruz Biotechnology stock bafilomycin a1
FIGURE 4 Areg deficiency upregulates autophagosome formation and inhibits autophagosome clearance. (A) LC3-II, p62, and GAPDH protein levels in the infarct border myocardium from WT and Areg−/− mice were quantified by western blotting on day 7 after MI. Left, representative immunoblot. Right, summary data. n = 4 per group. (B) WT and Areg−/− mice treated with 3-MA (30 μg/g/day) or vehicle were administered at the same time each day for 7 days after MI. LC3-II and GAPDH protein levels in the infarct border myocardium from WT and Areg−/− mice treated with 3-MA or vehicles were quantified by western blotting. Top, representative immunoblot. Bottom, summary data. n = 6 per group. (C) Seven days after MI, WT, and Areg−/− mice were injected with <t>BFA</t> (1.5 μg/g) or vehicles and sacrificed 2 h after for the assays. LC3-II and GAPDH protein levels in the infarct border myocardium from WT and Areg−/− mice treated with BFA or vehicles were quantified by western blotting. Top, representative immunoblot. Bottom, summary data. n = 5 per group. (D) Electron micrographs of the infarct border myocardium from WT (left) and Areg−/− (middle) hearts, were analyzed for the autophagic vacuole density (right). n = 4–5 per group. Scale bar, 1 μm. Data are expressed as means ± SEM. *p < .05, **p < .01. Data in (A) were analyzed by a two-tailed unpaired t-test. One-way ANOVA with the Tukey post hoc test was performed in (B) and (C). Data in (D) were analyzed with the Mann–Whitney test. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; i.p., intraperitoneal injection; 3-MA, 3-methyladenine; BFA, <t>bafilomycin-A1.</t>
Stock Bafilomycin A1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bafilomycin+a1+bfa/Bafilomycin+A1/pmc03770741-121-1-7
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bafilomycin a1 (bfa)  (LC Laboratories)
90
LC Laboratories bafilomycin a1 (bfa)
FIGURE 4 Areg deficiency upregulates autophagosome formation and inhibits autophagosome clearance. (A) LC3-II, p62, and GAPDH protein levels in the infarct border myocardium from WT and Areg−/− mice were quantified by western blotting on day 7 after MI. Left, representative immunoblot. Right, summary data. n = 4 per group. (B) WT and Areg−/− mice treated with 3-MA (30 μg/g/day) or vehicle were administered at the same time each day for 7 days after MI. LC3-II and GAPDH protein levels in the infarct border myocardium from WT and Areg−/− mice treated with 3-MA or vehicles were quantified by western blotting. Top, representative immunoblot. Bottom, summary data. n = 6 per group. (C) Seven days after MI, WT, and Areg−/− mice were injected with <t>BFA</t> (1.5 μg/g) or vehicles and sacrificed 2 h after for the assays. LC3-II and GAPDH protein levels in the infarct border myocardium from WT and Areg−/− mice treated with BFA or vehicles were quantified by western blotting. Top, representative immunoblot. Bottom, summary data. n = 5 per group. (D) Electron micrographs of the infarct border myocardium from WT (left) and Areg−/− (middle) hearts, were analyzed for the autophagic vacuole density (right). n = 4–5 per group. Scale bar, 1 μm. Data are expressed as means ± SEM. *p < .05, **p < .01. Data in (A) were analyzed by a two-tailed unpaired t-test. One-way ANOVA with the Tukey post hoc test was performed in (B) and (C). Data in (D) were analyzed with the Mann–Whitney test. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; i.p., intraperitoneal injection; 3-MA, 3-methyladenine; BFA, <t>bafilomycin-A1.</t>
Bafilomycin A1 (Bfa), supplied by LC Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bafilomycin+a1+bfa/bafilomycin+a1/pmc03310694-51-0-6
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bafilomycin a1 bfa  (BOC Sciences)
90
BOC Sciences bafilomycin a1 bfa
FIGURE 4 Areg deficiency upregulates autophagosome formation and inhibits autophagosome clearance. (A) LC3-II, p62, and GAPDH protein levels in the infarct border myocardium from WT and Areg−/− mice were quantified by western blotting on day 7 after MI. Left, representative immunoblot. Right, summary data. n = 4 per group. (B) WT and Areg−/− mice treated with 3-MA (30 μg/g/day) or vehicle were administered at the same time each day for 7 days after MI. LC3-II and GAPDH protein levels in the infarct border myocardium from WT and Areg−/− mice treated with 3-MA or vehicles were quantified by western blotting. Top, representative immunoblot. Bottom, summary data. n = 6 per group. (C) Seven days after MI, WT, and Areg−/− mice were injected with <t>BFA</t> (1.5 μg/g) or vehicles and sacrificed 2 h after for the assays. LC3-II and GAPDH protein levels in the infarct border myocardium from WT and Areg−/− mice treated with BFA or vehicles were quantified by western blotting. Top, representative immunoblot. Bottom, summary data. n = 5 per group. (D) Electron micrographs of the infarct border myocardium from WT (left) and Areg−/− (middle) hearts, were analyzed for the autophagic vacuole density (right). n = 4–5 per group. Scale bar, 1 μm. Data are expressed as means ± SEM. *p < .05, **p < .01. Data in (A) were analyzed by a two-tailed unpaired t-test. One-way ANOVA with the Tukey post hoc test was performed in (B) and (C). Data in (D) were analyzed with the Mann–Whitney test. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; i.p., intraperitoneal injection; 3-MA, 3-methyladenine; BFA, <t>bafilomycin-A1.</t>
Bafilomycin A1 Bfa, supplied by BOC Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bafilomycin+a1+bfa/Bafilomycin+A1/pm27113027-34-14-19
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Image Search Results


FIGURE 4 Areg deficiency upregulates autophagosome formation and inhibits autophagosome clearance. (A) LC3-II, p62, and GAPDH protein levels in the infarct border myocardium from WT and Areg−/− mice were quantified by western blotting on day 7 after MI. Left, representative immunoblot. Right, summary data. n = 4 per group. (B) WT and Areg−/− mice treated with 3-MA (30 μg/g/day) or vehicle were administered at the same time each day for 7 days after MI. LC3-II and GAPDH protein levels in the infarct border myocardium from WT and Areg−/− mice treated with 3-MA or vehicles were quantified by western blotting. Top, representative immunoblot. Bottom, summary data. n = 6 per group. (C) Seven days after MI, WT, and Areg−/− mice were injected with BFA (1.5 μg/g) or vehicles and sacrificed 2 h after for the assays. LC3-II and GAPDH protein levels in the infarct border myocardium from WT and Areg−/− mice treated with BFA or vehicles were quantified by western blotting. Top, representative immunoblot. Bottom, summary data. n = 5 per group. (D) Electron micrographs of the infarct border myocardium from WT (left) and Areg−/− (middle) hearts, were analyzed for the autophagic vacuole density (right). n = 4–5 per group. Scale bar, 1 μm. Data are expressed as means ± SEM. *p < .05, **p < .01. Data in (A) were analyzed by a two-tailed unpaired t-test. One-way ANOVA with the Tukey post hoc test was performed in (B) and (C). Data in (D) were analyzed with the Mann–Whitney test. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; i.p., intraperitoneal injection; 3-MA, 3-methyladenine; BFA, bafilomycin-A1.

Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Article Title: Amphiregulin improves ventricular remodeling after myocardial infarction by modulating autophagy and apoptosis.

doi: 10.1096/fj.202302385R

Figure Lengend Snippet: FIGURE 4 Areg deficiency upregulates autophagosome formation and inhibits autophagosome clearance. (A) LC3-II, p62, and GAPDH protein levels in the infarct border myocardium from WT and Areg−/− mice were quantified by western blotting on day 7 after MI. Left, representative immunoblot. Right, summary data. n = 4 per group. (B) WT and Areg−/− mice treated with 3-MA (30 μg/g/day) or vehicle were administered at the same time each day for 7 days after MI. LC3-II and GAPDH protein levels in the infarct border myocardium from WT and Areg−/− mice treated with 3-MA or vehicles were quantified by western blotting. Top, representative immunoblot. Bottom, summary data. n = 6 per group. (C) Seven days after MI, WT, and Areg−/− mice were injected with BFA (1.5 μg/g) or vehicles and sacrificed 2 h after for the assays. LC3-II and GAPDH protein levels in the infarct border myocardium from WT and Areg−/− mice treated with BFA or vehicles were quantified by western blotting. Top, representative immunoblot. Bottom, summary data. n = 5 per group. (D) Electron micrographs of the infarct border myocardium from WT (left) and Areg−/− (middle) hearts, were analyzed for the autophagic vacuole density (right). n = 4–5 per group. Scale bar, 1 μm. Data are expressed as means ± SEM. *p < .05, **p < .01. Data in (A) were analyzed by a two-tailed unpaired t-test. One-way ANOVA with the Tukey post hoc test was performed in (B) and (C). Data in (D) were analyzed with the Mann–Whitney test. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; i.p., intraperitoneal injection; 3-MA, 3-methyladenine; BFA, bafilomycin-A1.

Article Snippet: Bafilomycin- A1 (BFA) (No. HY- 100558; MedChemExpress) was dissolved in corn oil.

Techniques: Western Blot, Injection, Two Tailed Test, MANN-WHITNEY