Journal: Life

Article Title: 1,2,3-Triazole-Benzofused Molecular Conjugates as Potential Antiviral Agents against SARS-CoV-2 Virus Variants

doi: 10.3390/life12091341

Figure Lengend Snippet: Three-dimensional binding mode of compound 9 (green) at the binding interface between the SARS-CoV-2 S-RBD (red) and human ACE2 (blue).

Article Snippet: SARS-CoV-2 assay kit (SARS-CoV-2 Assay Kit, Biosource, Muskego, WI, USA) and the Omicron variant Spike RBD inhibitor screening kits (B.1.1.529 BA.1, Omicron Variant Assay Kit, BPS Bioscience, San Diego, CA, USA) were used to evaluate the anti-SARS-CoV-2 activity in vitro.

Techniques: Binding Assay

Journal: Cell Reports Medicine

Article Title: SARS-CoV-2 breakthrough infections enhance T cell response magnitude, breadth, and epitope repertoire

doi: 10.1016/j.xcrm.2024.101583

Figure Lengend Snippet: T and B cell responses in BTI donors as a function of Delta or Omicron SARS-CoV-2 variant wave T and B cell responses assessed in a cross-sectional cohort (cohort 2, Table S1 ) of BTI donors infected during Delta ( n = 24 at 3 months post symptoms onset [PSO]), Omicron BA.1 ( n = 20 at 1 month PSO, n = 32 at 3 months PSO), or Omicron BA.5 ( n = 19) SARS2 variant waves. (A) AIM+CD4 + T cell responses to S and CD4-RE are displayed on a two-dimensional plot, categorized by response type (none, S only, non-S only, non-S > S, non-S < S) with color-coded variant groups (Delta, orange; Omicron BA.1, dark blue; Omicron BA.5, purple). p values are shown for the χ 2 to assess response distribution across categories T cell reactivity. The percentage of each donor sample cohort in the different categories is shown as a bar graph. (B and C) AIM+CD4 + and CD8 + T cell response to ancestral S comparing (B) Delta versus Omicron BA.1 or (C) BA.1 versus BA.5 BTI. Bars represent the geometric mean and p values are shown for Mann-Whitney tests. (D and E) (D) Frequency of ancestral (Anc) RBD-binding B Mem (among total B cells) for Delta or BA.1 donors and (E) composition of B Mem subsets among ancestral, Delta or BA.1 RBD-binding B Mem in Delta or Omicron BA.1 BTI donors. (F and G) (F) Ancestral RBD IgG binding endpoint titers and (G) neutralization of SARS2 ancestral S PSV in Delta or Omicron donors. p values are shown for paired Wilcoxon t tests for the magnitude graphs. The y-axis begins at the LOD, and the LOS is indicated by the dotted line. See also Figures S1 , , and Table S1 .

Article Snippet: Omicron BA.1 RBD protein for ELISA , AcroBiosystems , Cat# SPD-C522j.

Techniques: Variant Assay, Infection, MANN-WHITNEY, Binding Assay, Neutralization

Journal: Cell Reports Medicine

Article Title: SARS-CoV-2 breakthrough infections enhance T cell response magnitude, breadth, and epitope repertoire

doi: 10.1016/j.xcrm.2024.101583

Figure Lengend Snippet: Impact of variant sequences on CD4 + and CD8 + T cell epitopes in Delta and Omicron BTI donors SARS-CoV-2-specific T cell epitopes in Delta ( n = 5; orange) and Omicron BA.1 ( n = 5; dark blue) BTI individuals (cohort 3 Table S1 ). (A–D) CD4 + T cell epitopes include conserved (A and C) and mutation-affected (B and D). Mutation impacts are categorized by response changes: increased, unchanged, decreased, or novel. (E–H) CD8 + T cell epitopes include conserved (E and G) and mutation affected (F and H) and categorized responses. The number of epitopes is indicated at the top of each graph. The dotted line represents the LOS, and the y-axis begins at the LOD. See also Figure S1 and Tables S1 , , and .

Article Snippet: Omicron BA.1 RBD protein for ELISA , AcroBiosystems , Cat# SPD-C522j.

Techniques: Variant Assay, Mutagenesis

Journal: Cell Reports Medicine

Article Title: SARS-CoV-2 breakthrough infections enhance T cell response magnitude, breadth, and epitope repertoire

doi: 10.1016/j.xcrm.2024.101583

Figure Lengend Snippet: Impact of variant sequences on the total T and B cell responses in Delta and Omicron BTI donors SARS2-specifc T cell responses in the BTI cohorts collected post infection from this study ( Table S1 ; Delta n = 24; Omicron BA.1 n = 68) measuring S and CD4-RE MPs. Responses to variant-specific mutations are analyzed using ancestral and variant peptide pools. (A and C) SARS-CoV-2-specific T cells in total (S + non-S) responses (light gray) compared to peptides affected by Delta (A) or Omicron BA.1 (C) mutations, using ancestral (A in dark gray; C in brown) and variant-specific peptides (Delta in orange, A; Omicron in blue, C). (B and D) The bar graph displays the percentage of preserved response per donor, calculated by subtracting the response to the ancestral pool affected by Delta (B) or Omicron BA.1 (D) mutations from the total (S + CD4-RE). The pie chart illustrates the percentage of individuals showing no change (± 0.005%), increase, novel, or decreased responses when comparing the ancestral and variant-specific peptide pools. (E) Ancestral and variant RBD-binding B Mem cells are plotted for their cross-reactive and non-cross-reactive binding in switched B cells (IgD−IgM−) for Delta ( n = 19) and Omicron BA.1 ( n = 20) BTI donors of cohort 2 ( Table S1 ). p values result from the paired Wilcoxon test and the comparison between ancestral and variant sequences is performed only if at least one value is above the LOS. Bars represent the geometric mean. Graphs start the LOD and the dotted line represents the LOS. See also Figure S1 and Table S1 .

Article Snippet: Omicron BA.1 RBD protein for ELISA , AcroBiosystems , Cat# SPD-C522j.

Techniques: Variant Assay, Infection, Binding Assay, Comparison

Journal: Cell Reports Medicine

Article Title: SARS-CoV-2 breakthrough infections enhance T cell response magnitude, breadth, and epitope repertoire

doi: 10.1016/j.xcrm.2024.101583

Figure Lengend Snippet:

Article Snippet: Omicron BA.1 RBD protein for ELISA , AcroBiosystems , Cat# SPD-C522j.

Techniques: Virus, Recombinant, Staining, Enzyme-linked Immunosorbent Assay, Mutagenesis, Software

Journal: iScience

Article Title: BA.1 breakthrough infection elicits distinct antibody and memory B cell responses in vaccinated-only versus hybrid immunity individuals

doi: 10.1016/j.isci.2025.111962

Figure Lengend Snippet:

Article Snippet: Recombinant SARS-CoV-2 RBD B.1.1.529/BA.1 omicron (HEK)-Biotin 50ug , Miltenyi , 130-130-419.

Techniques: Recombinant, Bioassay, Luciferase, Software, Imaging, Staining, Blocking Assay