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Image Search Results
Journal: bioRxiv
Article Title: Delivery of loaded MR1 Monomer Results in Efficient Ligand Exchange to Host MR1 and Subsequent MR1T cell activation
doi: 10.1101/2022.07.11.499573
Figure Lengend Snippet: A . Biotinylated MR1 monomer, recombinant TCR, or HLA-A2 were immobilized on a traptavidin-coated plate before incubation with PBS at the indicated pH overnight at 4C. Binding of either anti-MR1 (clone 26.5) or anti-b2m (clone B2M-01) antibody was assess with an HRP-conjugated secondary antibody and TMB substrate. Error bars denote standard deviation. B . MR1T clone (5e3) IFN-γ response to BEAS-2B (1e4) pre-treated with Bafilomycin A1 for 2 hours, followed by addition of the MR1/5-OP-RU monomer or 5-OP-RU prodrug. Data in B are representative of three independent experiments.
Article Snippet: After removal of the acidic buffer and washing with PBS pH 7.2, either anti-human MR1 (unlabeled, clone 26.5, BioLegend 361102) or
Techniques: Recombinant, Incubation, Binding Assay, Standard Deviation