Journal: Cell reports

Article Title: Chronic stress physically spares but functionally impairs innate-like invariant T cells

doi: 10.1016/j.celrep.2021.108979

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Article Snippet: Mouse: B16-F10-Red-FLuc (B16-FLuc) melanoma cells , PerkinElmer , Cat # BW124734.

Techniques: Control, Recombinant, Western Blot, Enzyme-linked Immunosorbent Assay, Staining, In Situ, Selection, cDNA Synthesis, Software

Journal: Journal of controlled release : official journal of the Controlled Release Society

Article Title: Surface Engineering Tumor Cells with Adjuvant-loaded Particles for Use as Cancer Vaccines

doi: 10.1016/j.jconrel.2016.12.036

Figure Lengend Snippet: (A1) B16.F10 cells and (A2) RM11 cells treated or not treated (control) with biotinylated anti-CD29 antibodies (anti-CD29-biotin) followed by strepavidin-PE. (B–F) Validation of cell-particle hybrid assembly from B16.F10 or RM11 cells subsequent to surface biotinylation using anti-CD29-biotin and mixed with streptavidin-coated particles loaded with rhodamine B and washed to remove unbound particles (hybrid). Control involved the same conditions except cells were not treated with anti-CD29-biotin. Validation was performed using: (B1–2 and E1–2): flow cytometry where (B1 and E1) representive (n = 1) and (B2 and E2) mean (n = 3) results from measuring relative mean rhodamine fluorescence intensity (RMFI(Rh)) of (B1 and B2) B16.F10 and (E1 and E2) RM11 cells; (C1–2 and F1–2): laser scanning confocal microscopy showing (C1 and F1) hybrid and (C2 and F2) control cell-particle mixtures for (C1 and C2) B16.F10 and (F1 and F2) RM11 cells (blue = DAPI stained cell nuclei, red = rhodamine-labeled PLGA particles); (D): scanning electron microscopy showing (D1) B16.F10 hybrid and (D2) control cell-particle mixtures (arrows in C1, D1, and F1 indicate particles bound to cell surface). Scale bar= 20 micron for C1–2 and F1–2, 10 microns for D1–2. When applicable, error bars = SD. ** p < 0.01, *** p < 0.001, n = 3.

Article Snippet: For vaccinations, a B16.F10 GM-CSF clone expressing 220 ng GM-CSF/10 6 cells/day was derived by transducing B16.F10 cells with a lentiviral vector encoding murine GM-CSF (AMSBIO, Cambridge, MA).

Techniques: Flow Cytometry, Fluorescence, Confocal Microscopy, Staining, Labeling, Electron Microscopy

Journal: Journal of controlled release : official journal of the Controlled Release Society

Article Title: Surface Engineering Tumor Cells with Adjuvant-loaded Particles for Use as Cancer Vaccines

doi: 10.1016/j.jconrel.2016.12.036

Figure Lengend Snippet: Laser scanning confocal microscopy imaging of cell-particle hybrid uptake by BMDC. Cell-particle hybrids were incubated with BMDC Yellow arrows indicating colocalization of B16.F10 cells (green) and particles (red) inside BMDC (magenta). Magenta: Alexa flour®700 (CD11c) stained BMDC, green: CFSE labeled B16.F10 melanoma cells, red: rhodamine B-labeled PLGA particles, gray: DAPI stained cell nuclei. Scale bar: 20 micron.

Article Snippet: For vaccinations, a B16.F10 GM-CSF clone expressing 220 ng GM-CSF/10 6 cells/day was derived by transducing B16.F10 cells with a lentiviral vector encoding murine GM-CSF (AMSBIO, Cambridge, MA).

Techniques: Confocal Microscopy, Imaging, Incubation, Staining, Labeling