Journal: Cell communication and signaling : CCS

Article Title: BMX, a specific HDAC8 inhibitor, with TMZ for advanced CRC therapy: a novel synergic effect to elicit p53-, β-catenin- and MGMT-dependent apoptotic cell death.

doi: 10.1186/s12964-022-01007-x

Figure Lengend Snippet: Fig. 1 BMX, TMZ, oxaliplatin (Oxp) and doxorubicin (Dox) combination inhibited cell proliferation in CRC cells. (A) The proliferation of BMX, TMZ, Oxp, Dox, BMX plus TMZ, BMX plus Oxp or BMX plus Dox in HT29, HCT116 and RKO cells with various drug concentration. (B) Colony formation capability assay with different treatments of BMX, TMZ, Oxp, BMX plus TMZ, and BMX plus Oxp in HT29, HCT116 and RKO cells; the colonies were counted for quantification. (C) Cell cycle analysis after 48 h treatment with different concentrations of BMX alone or BMX combined with TMZ in HT29, HCT116, and RKO cells and the proportion of cells in each cell cycle phase. SubG1, cell with polyploid chromosome; > 4 N, polyploid cell. (D) Apoptosis analysis after 48 h treatment with different concentrations of BMX alone or BMX combined with TMZ and the apoptotic rate of cells in HT29, HCT116, and RKO cells. All results are shown as mean ± s.d. from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 versus control (HT29 cells); #p < 0.05, ##p < 0.01, ###p < 0.001 vs. control (HCT116 cells); †p < 0.05, ††p < 0.01, †††p < 0.001 versus control (RKO cells)

Article Snippet: The cell proliferation of CRC cells was measured by CCK8 assay (Targetmol, Shanghai, China).

Techniques: Concentration Assay, Cell Cycle Assay, Control

Journal: Cell communication and signaling : CCS

Article Title: BMX, a specific HDAC8 inhibitor, with TMZ for advanced CRC therapy: a novel synergic effect to elicit p53-, β-catenin- and MGMT-dependent apoptotic cell death.

doi: 10.1186/s12964-022-01007-x

Figure Lengend Snippet: Fig. 5 BMX, VPA, SAHA, TMZ, Oxp, and Dox combination inhibited cell proliferation in CRC cells. (A) HDAC8 mRNA expression levels stimulated with BMX (5 µM), VPA (4 mM), SAHA (2 µM) with or without TMZ were determined using qRT-PCR assays. (B) HT29, HCT116, and RKO cells were treated with BMX with or without TMZ for 48 h. Then, cells were harvested for detection of acetyl-histone H3 (Lys9/Lys14), acetyl-histone H4 (Lys8), and HDAC8 by Western blot analysis. (C) The proliferation of BMX, VPA, SAHA with or without TMZ, Oxp (5 µM) and Dox (1 µM) in HT29, HCT116, and RKO cells with treatment durations were assayed using the CCK-8 method. (D) Colony formation capability assay with different treatments of BMX, VPA, SAHA with or without TMZ, Oxp and Dox in HT29, HCT116, and RKO cells; the colonies were counted for quantification. All results are shown as mean ± s.d. from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 versus control (HT29 cells); #p < 0.05, ##p < 0.01, ###p < 0.001 versus control (HCT116 cells); †p < 0.05, ††p < 0.01, †††p < 0.001 versus control (RKO cells)

Article Snippet: The cell proliferation of CRC cells was measured by CCK8 assay (Targetmol, Shanghai, China).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, CCK-8 Assay, Control

Journal: Cell communication and signaling : CCS

Article Title: BMX, a specific HDAC8 inhibitor, with TMZ for advanced CRC therapy: a novel synergic effect to elicit p53-, β-catenin- and MGMT-dependent apoptotic cell death.

doi: 10.1186/s12964-022-01007-x

Figure Lengend Snippet: Fig. 7 Autophagy expression levels stimulated with BMX and PCI-34051 in the presence or absence of TMZ. (A) HT29, HCT116, and RKO cells were treated with BMX and PCI-34051 with or without TMZ for 48 h. Then, cells were harvested for detection of acetyl-histone H3 (Lys9/Lys14), acetyl-histone H4 (Lys8), and HDAC8 by Western blot analysis. (B) The proliferation of BMX and PCI-34051 with or without TMZ in HT29, HCT116, and RKO cells. (C) Colony formation capability assay with different treatments of BMX and PCI-34051 with or without TMZ in HT29, HCT116, and RKO cells; the colonies were counted for quantification. (D, E) Expressions of cleaved caspase 3, cleaved PARP, LC3 and p62 proteins in HT29, HCT116, and RKO cells treated with BMX and PCI-34051 with or without TMZ for 48 h. All results are shown as mean ± s.d. from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 versus control (HT29 cells); #p < 0.05, ##p < 0.01, ###p < 0.001 versus control (HCT116 cells); †p < 0.05, ††p < 0.01, †††p < 0.001 versus control (RKO cells)

Article Snippet: The cell proliferation of CRC cells was measured by CCK8 assay (Targetmol, Shanghai, China).

Techniques: Expressing, Western Blot, Control

Journal: Cell Reports Medicine

Article Title: Ongoing evolution of SARS-CoV-2 drives escape from mRNA vaccine-induced humoral immunity

doi: 10.1016/j.xcrm.2024.101850

Figure Lengend Snippet:

Article Snippet: pTwist-SARS-CoV-2 Δ18 B.1.351v2 (spike) , This study , Addgene Plasmid ID 169463.

Techniques: Virus, Recombinant, Saline, Modification, Plasmid Preparation, Software, Control

Journal: International Journal of Environmental Research and Public Health

Article Title: Evaluation of Changes in Gut Microbiota in Patients with Crohn’s Disease after Anti-Tnfα Treatment: Prospective Multicenter Observational Study

doi: 10.3390/ijerph17145120

Figure Lengend Snippet: Summary of variables to be collected during the study.

Article Snippet: The samples will be coded and the bacterial microbiota present will be analyzed using capture of the v3-v4 region of the 16S rRNA subunit [ ], ultra-sequencing with Library Illumina 15044223 B protocol (ILLUMINA) comparative bioinformatics analysis.

Techniques: Biomarker Discovery

Journal: Blood

Article Title: Single-cell genomics details the maturation block in BCP-ALL and identifies therapeutic vulnerabilities in DUX4 -r cases

doi: 10.1182/blood.2023021705

Figure Lengend Snippet: Multimodal single-cell sequencing reveals a severely altered leukemic BM. (A) Schematic illustration of multiomic profiling of BCP-ALL (n = 23) and NBM (n = 9) samples. Illustration created with BioRender.com . (B) UMAP visualization of scRNA-seq gene expression data representing 188 546 cells from the BCP-ALL and NBM samples. Each cell is colored according to the cell type. (C) UMAP visualization of scATAC-seq data representing 41 892 nuclei from the BCP-ALL and NBM samples. (D) Cell composition based on scRNA-seq data in DUX4 -r, BCR :: ABL1 + , ETV6 :: RUNX1 + , and HeH ALL samples, as well as mononuclear cells (MNC), CD34 + -enriched cells, and CD19 + -enriched cells from NBM samples. ALL blast cells, regardless of subtype, are marked in gray. (E) Protein expression by scADT-seq of CD10, CD19, CD22, CD34, CD20, and CD45 on BCP-ALL blast cells and nonleukemic cells from BCP-ALL and NBM samples, visualized using ridge plots. (F) TF footprinting of the DUX4-binding motif shows that DUX4 is exclusively active in DUX4 -r blast cells (marked in purple). The annotation “all other cell types” denotes all other detected cell types using the color legend from C. (G) UMAP visualization of T cells extracted from the larger scRNA-seq data set colored by sample type (upper) and predicted cell type (lower) showing a specific cell cluster of an accumulation of CD4 + T cells exclusively from DUX4 -r ALL. (H) Gene set enrichment scores using defined gene sets from dysfunctional CD4 + T cells in cancer by Li et al (left) and Zheng et al (right). , The gene set enrichment scores are based on differentially expressed genes between memory CD4 + T cells in DUX4 -r ALL and NBM-MNC. GMP, granulocyte-macrophage progenitors; LMPP, lympho-myeloid primed progenitor; NK, natural killer; scBCR-seq, single-cell BCR sequencing; scRNA-seq, single-cell RNA sequencing; UMAP, uniform manifold approximation and projection.

Article Snippet: Sorted cells were used for single-cell antibody-derived tag sequencing (scADT-seq), single-cell B-cell receptor sequencing (scBCR-seq), and single-cell RNA-sequencing (scRNA-seq) library preparation according to the manufacturer’s protocol (10X Genomics).

Techniques: Sequencing, Expressing, Footprinting, Binding Assay, RNA Sequencing Assay