Journal: Pharmaceuticals

Article Title: Three-Dimensional Analysis of Sex- and Gonadal Status- Dependent Microglial Activation in a Mouse Model of Parkinson’s Disease

doi: 10.3390/ph16020152

Figure Lengend Snippet: Effect of mice gonadal status (SHAM, GDX), MPTP, and dutasteride (DUT) treatment on TH striatal levels. ( A ) Representative example of striatal TH immunofluorescence. Scale bar = 1000 μm and ( B ) TH striatal intensity in SHAM and GDX males and SHAM and GDX female mice. Values shown are the means pixel intensity ± SEM of 6-16 mice per group. Two-way ANOVA, Tukey post-hoc test: * p < 0.05, ** p < 0.01 and *** p < 0.001.

Article Snippet: Mice that received dutasteride (Toronto Research Chemicals, Toronto, ON, Canada) (5 mg/kg, intraperitoneal) or vehicle only (0.9% saline with 1% tween 80, i.p.) were injected once daily for 10 days.

Techniques: Immunofluorescence

Journal: Pharmaceuticals

Article Title: Three-Dimensional Analysis of Sex- and Gonadal Status- Dependent Microglial Activation in a Mouse Model of Parkinson’s Disease

doi: 10.3390/ph16020152

Figure Lengend Snippet: Effect of mice gonadal status (SHAM, GDX), MPTP, and dutasteride (DUT) treatment on striatal microglial density. ( A ) Representative example of striatal Iba1 immunofluorescence. Scale bar = 20 μm; ( B ) Iba1 positive cells count in the striatum in SHAM and GDX males and SHAM and GDX female mice. Values shown are the mean number of Iba1 positive cells per mm 3 ± SEM of 4 images of 4-6 mice per group and 7 to 25 microglia per image. Two-way ANOVA, Tukey post-hoc test: * p < 0.05 and ** p < 0.01 and ( C ) Correlations between striatal DA contents with striatal microglial density (Iba1 positive cell counts).

Article Snippet: Mice that received dutasteride (Toronto Research Chemicals, Toronto, ON, Canada) (5 mg/kg, intraperitoneal) or vehicle only (0.9% saline with 1% tween 80, i.p.) were injected once daily for 10 days.

Techniques: Immunofluorescence

Journal: Pharmaceuticals

Article Title: Three-Dimensional Analysis of Sex- and Gonadal Status- Dependent Microglial Activation in a Mouse Model of Parkinson’s Disease

doi: 10.3390/ph16020152

Figure Lengend Snippet: Effect of mice gonadal status (SHAM, GDX), MPTP, and dutasteride (DUT) treatment on striatal microglial doublet. ( A ) Representative example of striatal microglial doublet indicated with white arrowheads. Scale bars = 5 μm; ( B ) Microglial doublet counts in the striatum in SHAM and GDX males and SHAM and GDX female mice. Values shown are the mean number of doublets per image ± SEM 4 images of 4-6 mice per group and from 0 to 4 microglial doublet per image. Two-way ANOVA, Tukey post-hoc test: * p < 0.05 and **** p < 0.0001 and ( C ) Correlations between striatal DA contents with striatal microglial doublet counts.

Article Snippet: Mice that received dutasteride (Toronto Research Chemicals, Toronto, ON, Canada) (5 mg/kg, intraperitoneal) or vehicle only (0.9% saline with 1% tween 80, i.p.) were injected once daily for 10 days.

Techniques:

Journal: Pharmaceuticals

Article Title: Three-Dimensional Analysis of Sex- and Gonadal Status- Dependent Microglial Activation in a Mouse Model of Parkinson’s Disease

doi: 10.3390/ph16020152

Figure Lengend Snippet: Three-dimension morphological analysis of striatal microglia schematic and effect of mice gonadal status (SHAM, GDX), MPTP, and dutasteride (DUT) treatment on striatal microglial morphology in 3D. ( A ) Number of arborization terminal points representing the number of ramifications terminal points in the whole microglia. Values shown are the sum of terminal points of microglia ± SEM ( B ) Number of arborization branch points representing the number of ramifications branching points in the whole microglia. Values shown are the sum of branch points of microglia ± SEM; ( C ) Number of arborization segments representing the number of ramification segments in the whole microglia. Values shown are the sum of the segment of microglia ± SEM; ( D ) Arborization length defined as the sum of the lengths of all branches within the entire microglia. Values shown are the sum of the length of each branch of each microglia ± SEM; ( E ) Sholl intersections defined as the sum of branches intersections with concentric circles (spheres in 3D) spaced 1 μm each. Values shown are the sum of Sholl intersection of each microglia ± SEM; ( F ) Radius expressed as maximum reach from the nucleus where the concentric circle (spheres in 3D) presenting the intersection of the branches. Values shown are concentric circle numbers having the last branch intersection of microglia. The mean and SEM of 4-6 mice per group and from 1 to 14 microglia per mice is shown. Each point represents one microglia. Two-way ANOVA, Tukey post-hoc test: * p < 0.05, ** p < 0.01 and *** p < 0.001.

Article Snippet: Mice that received dutasteride (Toronto Research Chemicals, Toronto, ON, Canada) (5 mg/kg, intraperitoneal) or vehicle only (0.9% saline with 1% tween 80, i.p.) were injected once daily for 10 days.

Techniques:

Journal: Pharmaceuticals

Article Title: Three-Dimensional Analysis of Sex- and Gonadal Status- Dependent Microglial Activation in a Mouse Model of Parkinson’s Disease

doi: 10.3390/ph16020152

Figure Lengend Snippet: Experimental design. Male and female mice were gonadectomized (GDX) or sham operated (SHAM) and received intraperitoneal injection of dutasteride (DUT) or vehicle and 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine (MPTP) or saline.

Article Snippet: Mice that received dutasteride (Toronto Research Chemicals, Toronto, ON, Canada) (5 mg/kg, intraperitoneal) or vehicle only (0.9% saline with 1% tween 80, i.p.) were injected once daily for 10 days.

Techniques: Injection, Saline

Journal: Chemical & Biomedical Imaging

Article Title: Recombinant–Chemosynthetic Biosensors for Probing Cell Surface Signaling of Red Blood Cells and Other Cells

doi: 10.1021/cbmi.4c00067

Figure Lengend Snippet: Figure 2. (A) Optimization of the cell labeling protocol in HeLa cells was performed under three different conditions: (left) cells are in MEM/ DFBS/PS containing HeLa media and purified sortase A and GCaMP6s-LPDTG proteins are in 1% PBS; (middle) cells and purified sortase A and GCaMP6s-LPDTG proteins are in 1% PBS; (right) cells are in 1% PBS and purified sortase A and GCaMP6s-LPDTG proteins are in elution buffer. (B) HeLa cells labeled with ExtraCal showed a reduction in ExtraCal fluorescence upon EDTA addition and its fluorescence intensity was increased upon addition of HBSS containing Ca2+ (n = 25 cells from 3 repeats). The plot shows fluorescence change of ExtraCal upon EDTA followed by HBSS-With- Ca2+ addition over the course of 6 min. The error bars represent SD (standard error of standard deviation). Scale bar = 5 μm. EDTA, ethylenediaminetetraacetic acid; HBSS, Hanks’ balanced salt solution.

Article Snippet: EDTA Disodium salt dihydrate (Fisher Scientific), HBSS-With-Ca2+ (Gibco), Ca2+ free HBSS (Gibco), ATP (Chem-Impex International, Inc.)), Rhod-2 AM (Cayman Chemical), YM-254890 (Cayman Chemical), oxyrase (Oxyrase, Inc.), MgCl2 hexahydrate (SigmaAldrich), PMSF (Research Products International), Lysozyme (Gold Biotechnology), Luciferin (Gold Biotechnology), and EZ-Run Prestained Rec Protein Ladder (Fisher Scientific).

Techniques: Labeling, Purification, Fluorescence, Standard Deviation

Journal: Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases

Article Title: A Novel Framework to Compare the Effectiveness of β-lactamase Inhibitors against Extended-Spectrum β-lactamase-producing Enterobacteriaceae

doi: 10.1016/j.cmi.2019.01.003

Figure Lengend Snippet: Simulated piperacillin and β-lactamase inhibitor exposures

Article Snippet: Tazobactam was purchased from Chem-Impex International (Wood Dale, IL).

Techniques:

Journal: Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases

Article Title: A Novel Framework to Compare the Effectiveness of β-lactamase Inhibitors against Extended-Spectrum β-lactamase-producing Enterobacteriaceae

doi: 10.1016/j.cmi.2019.01.003

Figure Lengend Snippet: Estimated E max model parameters, % f T> MIC i and predicted outcomes for tazobactam, relebactam and avibactam

Article Snippet: Tazobactam was purchased from Chem-Impex International (Wood Dale, IL).

Techniques: Bacteria

Journal: Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases

Article Title: A Novel Framework to Compare the Effectiveness of β-lactamase Inhibitors against Extended-Spectrum β-lactamase-producing Enterobacteriaceae

doi: 10.1016/j.cmi.2019.01.003

Figure Lengend Snippet: Killing profiles for piperacillin in combination with tazobactam and avibactam against KpK91(a) and Kp2301 (b).

Article Snippet: Tazobactam was purchased from Chem-Impex International (Wood Dale, IL).

Techniques: