axiovert 40 cfl inverted microscope Search Results


inverted cell culture axiovert 40 cfl microscope  (Carl Zeiss)
90
Carl Zeiss inverted cell culture axiovert 40 cfl microscope
Inverted Cell Culture Axiovert 40 Cfl Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fluorescent filter cubes cy5  (Carl Zeiss)
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Carl Zeiss fluorescent filter cubes cy5
A . qPCR analysis of relative mRNA expression revealed greater CRF 1 mRNA in females compared to males t(12)=2.728, P = 0.183, and equal CB 1 mRNA across sexes t(14) = 0.090, P = 0.929. B . RNAScope was performed for CRF 1 , CB 1 , and vglut1 <t>mRNAs.</t> <t>Fluorescent</t> grains were counted in the left and right hemispheres of the posterior insular cortex. The total number of cells was determined by counting <t>DAPI</t> nuclei in each hemisphere. Nuclei containing 3 or more fluorescent grains were considered mRNA expressing cells and shown as the % of the total cells. The number of vglut1 cells was equal between sexes, but the portion of cells expressing CRF 1 F Sex (1, 14) = 11.19, P = 0.005, or CB 1 , F Sex (1, 14) = 13.07, P = 0.003, mRNA was approximately double in males compared to females. C . Looking at expression of CRF 1 and CB 1 mRNA in vglut1 cells, shown as a percent of the total vglut1 cells per hemisphere, male rat sections contained more CB 1 mRNA expressing vglut cells, and more vglut cells expressing both CB 1 and CRF 1 mRNA, F Sex (1, 14) = 4.489, P = 0.044). The number of vglut cells expressing CRF1 mRNA was greater on average in males than females, but did not reach significance F Sex (1, 14) = 3.944, P = 0.067. In males, there were more vglut cells co-localized with both CRF 1 and CB 1 , F Sex (1, 14) = 6.576, P = 0.023. D-E . Representative digital photomicrographs of RNAScope in situ hybridization and fluorescent visualization of DAPI and vesicular glutamate transporter 1 ( vglut1) , CRF 1 (crhr1), and CB 1 (cn1r) mRNA from male (A) and female (rats) insular cortex coronal sections (20um, n=8/sex). Sections were selected from subjects nearest to the mean values for CRF 1 + DAPI colabeling. Colored arrowheads indicate cells with coexpression of all three mRNAs with DAPI. Bar graphs indicate mean (+SEM) with individual replicates. *P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 (Sidak’s tests).
Fluorescent Filter Cubes Cy5, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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axiovert 40 cfl inverted microscope equipped with an axiocam digital camera and imaging system  (Carl Zeiss)
90
Carl Zeiss axiovert 40 cfl inverted microscope equipped with an axiocam digital camera and imaging system
A . qPCR analysis of relative mRNA expression revealed greater CRF 1 mRNA in females compared to males t(12)=2.728, P = 0.183, and equal CB 1 mRNA across sexes t(14) = 0.090, P = 0.929. B . RNAScope was performed for CRF 1 , CB 1 , and vglut1 <t>mRNAs.</t> <t>Fluorescent</t> grains were counted in the left and right hemispheres of the posterior insular cortex. The total number of cells was determined by counting <t>DAPI</t> nuclei in each hemisphere. Nuclei containing 3 or more fluorescent grains were considered mRNA expressing cells and shown as the % of the total cells. The number of vglut1 cells was equal between sexes, but the portion of cells expressing CRF 1 F Sex (1, 14) = 11.19, P = 0.005, or CB 1 , F Sex (1, 14) = 13.07, P = 0.003, mRNA was approximately double in males compared to females. C . Looking at expression of CRF 1 and CB 1 mRNA in vglut1 cells, shown as a percent of the total vglut1 cells per hemisphere, male rat sections contained more CB 1 mRNA expressing vglut cells, and more vglut cells expressing both CB 1 and CRF 1 mRNA, F Sex (1, 14) = 4.489, P = 0.044). The number of vglut cells expressing CRF1 mRNA was greater on average in males than females, but did not reach significance F Sex (1, 14) = 3.944, P = 0.067. In males, there were more vglut cells co-localized with both CRF 1 and CB 1 , F Sex (1, 14) = 6.576, P = 0.023. D-E . Representative digital photomicrographs of RNAScope in situ hybridization and fluorescent visualization of DAPI and vesicular glutamate transporter 1 ( vglut1) , CRF 1 (crhr1), and CB 1 (cn1r) mRNA from male (A) and female (rats) insular cortex coronal sections (20um, n=8/sex). Sections were selected from subjects nearest to the mean values for CRF 1 + DAPI colabeling. Colored arrowheads indicate cells with coexpression of all three mRNAs with DAPI. Bar graphs indicate mean (+SEM) with individual replicates. *P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 (Sidak’s tests).
Axiovert 40 Cfl Inverted Microscope Equipped With An Axiocam Digital Camera And Imaging System, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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trinocular inverted phase-contrast fluorescence microscope carl zeiss axiovert 40 cfl  (Carl Zeiss)
90
Carl Zeiss trinocular inverted phase-contrast fluorescence microscope carl zeiss axiovert 40 cfl
A . qPCR analysis of relative mRNA expression revealed greater CRF 1 mRNA in females compared to males t(12)=2.728, P = 0.183, and equal CB 1 mRNA across sexes t(14) = 0.090, P = 0.929. B . RNAScope was performed for CRF 1 , CB 1 , and vglut1 <t>mRNAs.</t> <t>Fluorescent</t> grains were counted in the left and right hemispheres of the posterior insular cortex. The total number of cells was determined by counting <t>DAPI</t> nuclei in each hemisphere. Nuclei containing 3 or more fluorescent grains were considered mRNA expressing cells and shown as the % of the total cells. The number of vglut1 cells was equal between sexes, but the portion of cells expressing CRF 1 F Sex (1, 14) = 11.19, P = 0.005, or CB 1 , F Sex (1, 14) = 13.07, P = 0.003, mRNA was approximately double in males compared to females. C . Looking at expression of CRF 1 and CB 1 mRNA in vglut1 cells, shown as a percent of the total vglut1 cells per hemisphere, male rat sections contained more CB 1 mRNA expressing vglut cells, and more vglut cells expressing both CB 1 and CRF 1 mRNA, F Sex (1, 14) = 4.489, P = 0.044). The number of vglut cells expressing CRF1 mRNA was greater on average in males than females, but did not reach significance F Sex (1, 14) = 3.944, P = 0.067. In males, there were more vglut cells co-localized with both CRF 1 and CB 1 , F Sex (1, 14) = 6.576, P = 0.023. D-E . Representative digital photomicrographs of RNAScope in situ hybridization and fluorescent visualization of DAPI and vesicular glutamate transporter 1 ( vglut1) , CRF 1 (crhr1), and CB 1 (cn1r) mRNA from male (A) and female (rats) insular cortex coronal sections (20um, n=8/sex). Sections were selected from subjects nearest to the mean values for CRF 1 + DAPI colabeling. Colored arrowheads indicate cells with coexpression of all three mRNAs with DAPI. Bar graphs indicate mean (+SEM) with individual replicates. *P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 (Sidak’s tests).
Trinocular Inverted Phase Contrast Fluorescence Microscope Carl Zeiss Axiovert 40 Cfl, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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axiovert 40 cfl inverted compound light microscope camera attachment  (Carl Zeiss)
90
Carl Zeiss axiovert 40 cfl inverted compound light microscope camera attachment
A . qPCR analysis of relative mRNA expression revealed greater CRF 1 mRNA in females compared to males t(12)=2.728, P = 0.183, and equal CB 1 mRNA across sexes t(14) = 0.090, P = 0.929. B . RNAScope was performed for CRF 1 , CB 1 , and vglut1 <t>mRNAs.</t> <t>Fluorescent</t> grains were counted in the left and right hemispheres of the posterior insular cortex. The total number of cells was determined by counting <t>DAPI</t> nuclei in each hemisphere. Nuclei containing 3 or more fluorescent grains were considered mRNA expressing cells and shown as the % of the total cells. The number of vglut1 cells was equal between sexes, but the portion of cells expressing CRF 1 F Sex (1, 14) = 11.19, P = 0.005, or CB 1 , F Sex (1, 14) = 13.07, P = 0.003, mRNA was approximately double in males compared to females. C . Looking at expression of CRF 1 and CB 1 mRNA in vglut1 cells, shown as a percent of the total vglut1 cells per hemisphere, male rat sections contained more CB 1 mRNA expressing vglut cells, and more vglut cells expressing both CB 1 and CRF 1 mRNA, F Sex (1, 14) = 4.489, P = 0.044). The number of vglut cells expressing CRF1 mRNA was greater on average in males than females, but did not reach significance F Sex (1, 14) = 3.944, P = 0.067. In males, there were more vglut cells co-localized with both CRF 1 and CB 1 , F Sex (1, 14) = 6.576, P = 0.023. D-E . Representative digital photomicrographs of RNAScope in situ hybridization and fluorescent visualization of DAPI and vesicular glutamate transporter 1 ( vglut1) , CRF 1 (crhr1), and CB 1 (cn1r) mRNA from male (A) and female (rats) insular cortex coronal sections (20um, n=8/sex). Sections were selected from subjects nearest to the mean values for CRF 1 + DAPI colabeling. Colored arrowheads indicate cells with coexpression of all three mRNAs with DAPI. Bar graphs indicate mean (+SEM) with individual replicates. *P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 (Sidak’s tests).
Axiovert 40 Cfl Inverted Compound Light Microscope Camera Attachment, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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axiovert 40 cfl inverted light microscope with camera attachment  (Carl Zeiss)
90
Carl Zeiss axiovert 40 cfl inverted light microscope with camera attachment
A . qPCR analysis of relative mRNA expression revealed greater CRF 1 mRNA in females compared to males t(12)=2.728, P = 0.183, and equal CB 1 mRNA across sexes t(14) = 0.090, P = 0.929. B . RNAScope was performed for CRF 1 , CB 1 , and vglut1 <t>mRNAs.</t> <t>Fluorescent</t> grains were counted in the left and right hemispheres of the posterior insular cortex. The total number of cells was determined by counting <t>DAPI</t> nuclei in each hemisphere. Nuclei containing 3 or more fluorescent grains were considered mRNA expressing cells and shown as the % of the total cells. The number of vglut1 cells was equal between sexes, but the portion of cells expressing CRF 1 F Sex (1, 14) = 11.19, P = 0.005, or CB 1 , F Sex (1, 14) = 13.07, P = 0.003, mRNA was approximately double in males compared to females. C . Looking at expression of CRF 1 and CB 1 mRNA in vglut1 cells, shown as a percent of the total vglut1 cells per hemisphere, male rat sections contained more CB 1 mRNA expressing vglut cells, and more vglut cells expressing both CB 1 and CRF 1 mRNA, F Sex (1, 14) = 4.489, P = 0.044). The number of vglut cells expressing CRF1 mRNA was greater on average in males than females, but did not reach significance F Sex (1, 14) = 3.944, P = 0.067. In males, there were more vglut cells co-localized with both CRF 1 and CB 1 , F Sex (1, 14) = 6.576, P = 0.023. D-E . Representative digital photomicrographs of RNAScope in situ hybridization and fluorescent visualization of DAPI and vesicular glutamate transporter 1 ( vglut1) , CRF 1 (crhr1), and CB 1 (cn1r) mRNA from male (A) and female (rats) insular cortex coronal sections (20um, n=8/sex). Sections were selected from subjects nearest to the mean values for CRF 1 + DAPI colabeling. Colored arrowheads indicate cells with coexpression of all three mRNAs with DAPI. Bar graphs indicate mean (+SEM) with individual replicates. *P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 (Sidak’s tests).
Axiovert 40 Cfl Inverted Light Microscope With Camera Attachment, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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carl zeiss axiovert 40 cfl inverted microscope  (Fisher Scientific)
90
Fisher Scientific carl zeiss axiovert 40 cfl inverted microscope
A . qPCR analysis of relative mRNA expression revealed greater CRF 1 mRNA in females compared to males t(12)=2.728, P = 0.183, and equal CB 1 mRNA across sexes t(14) = 0.090, P = 0.929. B . RNAScope was performed for CRF 1 , CB 1 , and vglut1 <t>mRNAs.</t> <t>Fluorescent</t> grains were counted in the left and right hemispheres of the posterior insular cortex. The total number of cells was determined by counting <t>DAPI</t> nuclei in each hemisphere. Nuclei containing 3 or more fluorescent grains were considered mRNA expressing cells and shown as the % of the total cells. The number of vglut1 cells was equal between sexes, but the portion of cells expressing CRF 1 F Sex (1, 14) = 11.19, P = 0.005, or CB 1 , F Sex (1, 14) = 13.07, P = 0.003, mRNA was approximately double in males compared to females. C . Looking at expression of CRF 1 and CB 1 mRNA in vglut1 cells, shown as a percent of the total vglut1 cells per hemisphere, male rat sections contained more CB 1 mRNA expressing vglut cells, and more vglut cells expressing both CB 1 and CRF 1 mRNA, F Sex (1, 14) = 4.489, P = 0.044). The number of vglut cells expressing CRF1 mRNA was greater on average in males than females, but did not reach significance F Sex (1, 14) = 3.944, P = 0.067. In males, there were more vglut cells co-localized with both CRF 1 and CB 1 , F Sex (1, 14) = 6.576, P = 0.023. D-E . Representative digital photomicrographs of RNAScope in situ hybridization and fluorescent visualization of DAPI and vesicular glutamate transporter 1 ( vglut1) , CRF 1 (crhr1), and CB 1 (cn1r) mRNA from male (A) and female (rats) insular cortex coronal sections (20um, n=8/sex). Sections were selected from subjects nearest to the mean values for CRF 1 + DAPI colabeling. Colored arrowheads indicate cells with coexpression of all three mRNAs with DAPI. Bar graphs indicate mean (+SEM) with individual replicates. *P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 (Sidak’s tests).
Carl Zeiss Axiovert 40 Cfl Inverted Microscope, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A . qPCR analysis of relative mRNA expression revealed greater CRF 1 mRNA in females compared to males t(12)=2.728, P = 0.183, and equal CB 1 mRNA across sexes t(14) = 0.090, P = 0.929. B . RNAScope was performed for CRF 1 , CB 1 , and vglut1 mRNAs. Fluorescent grains were counted in the left and right hemispheres of the posterior insular cortex. The total number of cells was determined by counting DAPI nuclei in each hemisphere. Nuclei containing 3 or more fluorescent grains were considered mRNA expressing cells and shown as the % of the total cells. The number of vglut1 cells was equal between sexes, but the portion of cells expressing CRF 1 F Sex (1, 14) = 11.19, P = 0.005, or CB 1 , F Sex (1, 14) = 13.07, P = 0.003, mRNA was approximately double in males compared to females. C . Looking at expression of CRF 1 and CB 1 mRNA in vglut1 cells, shown as a percent of the total vglut1 cells per hemisphere, male rat sections contained more CB 1 mRNA expressing vglut cells, and more vglut cells expressing both CB 1 and CRF 1 mRNA, F Sex (1, 14) = 4.489, P = 0.044). The number of vglut cells expressing CRF1 mRNA was greater on average in males than females, but did not reach significance F Sex (1, 14) = 3.944, P = 0.067. In males, there were more vglut cells co-localized with both CRF 1 and CB 1 , F Sex (1, 14) = 6.576, P = 0.023. D-E . Representative digital photomicrographs of RNAScope in situ hybridization and fluorescent visualization of DAPI and vesicular glutamate transporter 1 ( vglut1) , CRF 1 (crhr1), and CB 1 (cn1r) mRNA from male (A) and female (rats) insular cortex coronal sections (20um, n=8/sex). Sections were selected from subjects nearest to the mean values for CRF 1 + DAPI colabeling. Colored arrowheads indicate cells with coexpression of all three mRNAs with DAPI. Bar graphs indicate mean (+SEM) with individual replicates. *P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 (Sidak’s tests).

Journal: bioRxiv

Article Title: Insular cortex corticotropin-releasing factor integrates stress signaling with social decision making

doi: 10.1101/2021.03.23.436680

Figure Lengend Snippet: A . qPCR analysis of relative mRNA expression revealed greater CRF 1 mRNA in females compared to males t(12)=2.728, P = 0.183, and equal CB 1 mRNA across sexes t(14) = 0.090, P = 0.929. B . RNAScope was performed for CRF 1 , CB 1 , and vglut1 mRNAs. Fluorescent grains were counted in the left and right hemispheres of the posterior insular cortex. The total number of cells was determined by counting DAPI nuclei in each hemisphere. Nuclei containing 3 or more fluorescent grains were considered mRNA expressing cells and shown as the % of the total cells. The number of vglut1 cells was equal between sexes, but the portion of cells expressing CRF 1 F Sex (1, 14) = 11.19, P = 0.005, or CB 1 , F Sex (1, 14) = 13.07, P = 0.003, mRNA was approximately double in males compared to females. C . Looking at expression of CRF 1 and CB 1 mRNA in vglut1 cells, shown as a percent of the total vglut1 cells per hemisphere, male rat sections contained more CB 1 mRNA expressing vglut cells, and more vglut cells expressing both CB 1 and CRF 1 mRNA, F Sex (1, 14) = 4.489, P = 0.044). The number of vglut cells expressing CRF1 mRNA was greater on average in males than females, but did not reach significance F Sex (1, 14) = 3.944, P = 0.067. In males, there were more vglut cells co-localized with both CRF 1 and CB 1 , F Sex (1, 14) = 6.576, P = 0.023. D-E . Representative digital photomicrographs of RNAScope in situ hybridization and fluorescent visualization of DAPI and vesicular glutamate transporter 1 ( vglut1) , CRF 1 (crhr1), and CB 1 (cn1r) mRNA from male (A) and female (rats) insular cortex coronal sections (20um, n=8/sex). Sections were selected from subjects nearest to the mean values for CRF 1 + DAPI colabeling. Colored arrowheads indicate cells with coexpression of all three mRNAs with DAPI. Bar graphs indicate mean (+SEM) with individual replicates. *P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 (Sidak’s tests).

Article Snippet: = 0.8) and fluorescent filter cubes for DAPI (365 nm excitation, Zeiss filter 49), GFP (470/40 nm excitation, Zeiss filter 38 HE), DSRed (545/25 nm excitation, Zeiss filter 43 HE) and Cy5 (640/30 nm excitation, Zeiss filter 50).

Techniques: Expressing, In Situ Hybridization