Journal: Scientific Reports

Article Title: The Wnt/β-catenin signaling pathway has a healing ability for periapical periodontitis

doi: 10.1038/s41598-021-99231-x

Figure Lengend Snippet: IWR-1 injection inhibited the Wnt/β-catenin signaling pathway and spread the periapical lesion. ( A ) The quantification of periapical lesion volumes of the vehicle group (white bar, mean ± SD, n = 4) and the IWR-1 treatment group (black bar, mean ± SD, n = 4). Two-tailed paired Student’s t test, * P < 0.05. ( B ) In situ hybridization for Axin2 in the periapical lesion at 4 weeks after chamber opening. R root, PL periapical lesion, AB alveolar bone. Scale bars = 100 μm.

Article Snippet: Prehybridization was carried out at 55 °C for 1 h, and hybridization was carried out overnight at 70 °C using digoxigenin-labeled RNA probes, Axin2 ; addgene #21277, NM_015732, nt1774-2787, Col1a1 ; M_007742, nt29553415, Runx2 ; AF010284, nt922_1746.

Techniques: Injection, Two Tailed Test, In Situ Hybridization

Journal: Scientific Reports

Article Title: The Wnt/β-catenin signaling pathway has a healing ability for periapical periodontitis

doi: 10.1038/s41598-021-99231-x

Figure Lengend Snippet: Activation of the Wnt/β-catenin signaling pathway reduced the periapical lesion volume. ( A ) Quantification of periapical lesion volumes of the control group (white bar, mean ± SD, n = 4) and the LiCl application group (black bar, mean ± SD, n = 4). Two-tailed paired Student’s t test, * P < 0.05. ( B ) In situ hybridization for Axin2 in the periapical lesion at 4 weeks after chamber opening. R root, PL periapical lesion. Scale bars = 50 μm.

Article Snippet: Prehybridization was carried out at 55 °C for 1 h, and hybridization was carried out overnight at 70 °C using digoxigenin-labeled RNA probes, Axin2 ; addgene #21277, NM_015732, nt1774-2787, Col1a1 ; M_007742, nt29553415, Runx2 ; AF010284, nt922_1746.

Techniques: Activation Assay, Control, Two Tailed Test, In Situ Hybridization

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Effect of Pulsed Electromagnetic Field on Bone Formation and Lipid Metabolism of Glucocorticoid-Induced Osteoporosis Rats through Canonical Wnt Signaling Pathway

doi: 10.1155/2016/4927035

Figure Lengend Snippet: Primer sequences for real-time PCR analysis.

Article Snippet: The PVDF membrane was blocked for 2 h at room temperature in TBS-Tween 20 (TBST) buffer containing 5% BSA, washed with TBST three times, and incubated overnight at 4°C with 1/500 dilution of Wnt10b antibodies, LRP5 antibodies, β -catenin antibodies, Axin2 antibodies, OPG antibodies, RANKL antibodies, Dkk-1 antibodies, SOST antibodies, Runx2 antibodies, PPAR- γ antibodies, C/EBP α antibodies, FABP4 antibodies (all purchased from Santa Cruz Biotech), and GAPDH antibody (1 : 1000, BOSTER, China), respectively.

Techniques: Real-time Polymerase Chain Reaction, Sequencing

Journal: Biochemical and biophysical research communications

Article Title: Nuclear AXIN2 represses MYC gene expression

doi: 10.1016/j.bbrc.2013.11.089

Figure Lengend Snippet: Subcellular localization of AXIN2 in established human CRC cell lines and intestinal tissues. (A) Western blot analysis of protein lysates prepared from HEK293 cells that were transfected with pcDNA3-NLS-AXIN2. (B) Western blot analysis of protein lysates prepared from HCT116 and SW480 cells that were transduced with lentiviruses expressing a scrambled sequence (Ctrl.) or four independent shRNAs designed to target AXIN2. (C) Western blot analysis of whole cell [W], cytoplasmic [C], and nuclear [N] lysates prepared from HCT116, SW480, and SW620 cell lines. (D) Immunocytochemical analysis of AXIN2 subcellular localization in HCT116, SW480, and SW620 CRC cell lines. (E) Immunohistochemical analysis of human tissue microarrays prepared from uninvolved colonic mucosa, primary colorectal adenomas, and colorectal tumor metastases. Shown are representative images. Grey and black arrows identify cells with cytoplasmic and nuclear AXIN2 staining, respectively.

Article Snippet: To generate the pcDNA3-NLS-AXIN2 expression vector, NLS-AXIN2 was first amplified using pCMV6-entry-AXIN2 (Origene, RC210931) as the template and the indicated primers ( Table S1 ) in standard PCR reactions containing Phusion polymerase (New England Biolabs).

Techniques: Western Blot, Transfection, Transduction, Expressing, Sequencing, Immunohistochemical staining, Staining

Journal: Biochemical and biophysical research communications

Article Title: Nuclear AXIN2 represses MYC gene expression

doi: 10.1016/j.bbrc.2013.11.089

Figure Lengend Snippet: Nuclear AXIN2 decreases the activity of a Wnt/β-catenin-responsive luciferase reporter. (A) Western blot analysis of whole cell [W], cytoplasmic [C], and nuclear [N] protein lysates prepared from HEK293 cells. (B) Western blot analysis of protein lysates prepared from cytoplasmic [C] and nuclear [N] compartments of HEK293 cells that were transfected with pcDNA3-NLS-AXIN2. (C) Luciferase reporter assays in HEK293 cells transfected with the Wnt-responsive TOPflash reporter or the control FOPflash reporter. Where indicated, cells were co-transfected with plasmids encoding TCF4, β-catenin S45F, and NLS-AXIN2. Data are represented as mean +/− SEM (n = 4, *P < 0.05)

Article Snippet: To generate the pcDNA3-NLS-AXIN2 expression vector, NLS-AXIN2 was first amplified using pCMV6-entry-AXIN2 (Origene, RC210931) as the template and the indicated primers ( Table S1 ) in standard PCR reactions containing Phusion polymerase (New England Biolabs).

Techniques: Activity Assay, Luciferase, Western Blot, Transfection

Journal: Biochemical and biophysical research communications

Article Title: Nuclear AXIN2 represses MYC gene expression

doi: 10.1016/j.bbrc.2013.11.089

Figure Lengend Snippet: β-Catenin bridges AXIN2 to TCF4. (A) Western blot analysis of proteins prepared from whole cell [W], cytoplasmic [C], and nuclear [N] compartments of HCT116 cells expressing NLS-AXIN2. (B) Top, Co-immunoprecipitation/western blot analysis of proteins precipitated with anti-AXIN2 antibodies in nuclear lysates prepared from HCT116 cells. Bottom, Western blot analysis of cytoplasmic [C] and nuclear [N] fractions using α-tubulin and histone H3 antibodies. (C) Co-immunoprecipitation/western blot analysis of TCF4 interacting proteins. HEK293 cells were transfected with plasmids expressing the indicated cDNAs and TCF4 was precipitated with anti-TCF4 antibodies.

Article Snippet: To generate the pcDNA3-NLS-AXIN2 expression vector, NLS-AXIN2 was first amplified using pCMV6-entry-AXIN2 (Origene, RC210931) as the template and the indicated primers ( Table S1 ) in standard PCR reactions containing Phusion polymerase (New England Biolabs).

Techniques: Western Blot, Expressing, Immunoprecipitation, Transfection

Journal: Biochemical and biophysical research communications

Article Title: Nuclear AXIN2 represses MYC gene expression

doi: 10.1016/j.bbrc.2013.11.089

Figure Lengend Snippet: AXIN2 alters the chromatin structure at the MYC promoter and decreases MYC expression. (A) Luciferase assays of HCT116 cells transfected with wild-type or mutant MYC 5’ WRE-containing luciferase reporters in the presence or absence of pcDNA3-NLS-AXIN2. (B) Diagram of the MYC gene locus with the MYC 5’ WRE represented as a white box, MYC exons as gray boxes, and introns as thick black lines. The positions of PCR primer sets used to interrogate DNA elements precipitated in the ChIP assays are indicated by opposing arrows. Control (Ctrl.) is a region approximately 287 kb upstream from the MYC transcription start site. (C) ChIP analysis of TCF4 (black bars), β-catenin (gray bars), and AXIN2 (white bars) binding to the MYC 5’ WRE in HCT116 cells. (D) ChIP analysis of AXIN2, β-catenin and H3K4me3, in HCT116 cells transfected with pcDNA3 (Ctrl.) or pcDNA3-NLS-AXIN2. (E) Real-time reverse transcription PCR (qRT-PCR) analysis of MYC expression in control or HCT116 cells expressing NLS-AXIN2. (C-E) Data are represented as mean +/− SEM (n = 4, **P < 0.01, *** P < 0.001).

Article Snippet: To generate the pcDNA3-NLS-AXIN2 expression vector, NLS-AXIN2 was first amplified using pCMV6-entry-AXIN2 (Origene, RC210931) as the template and the indicated primers ( Table S1 ) in standard PCR reactions containing Phusion polymerase (New England Biolabs).

Techniques: Expressing, Luciferase, Transfection, Mutagenesis, Binding Assay, Quantitative RT-PCR

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Transcriptomic Dissection of Hepatocyte Heterogeneity: Linking Ploidy, Zonation, and Stem/Progenitor Cell Characteristics

doi: 10.1016/j.jcmgh.2019.08.011

Figure Lengend Snippet: Gene List for scPCR Analysis

Article Snippet: Axin2 Rn00577441_m1 , , , , ○ , ○ , , Plays an important role in the regulation of the stability of β-catenin , , , .

Techniques: Marker, Modification, Conjugation Assay, Expressing, Clinical Proteomics, Produced, Negative Control

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Transcriptomic Dissection of Hepatocyte Heterogeneity: Linking Ploidy, Zonation, and Stem/Progenitor Cell Characteristics

doi: 10.1016/j.jcmgh.2019.08.011

Figure Lengend Snippet: Characterization of 2c and 4c hepatocytes by PCA mapping. ( A ) PCA mapping of 92 2c (green) and 245 4c (blue) hepatocytes. ( B–D ) Each cell is colored according to the designated gene expression level as scaled with the color key. ( B ) Solid ellipses and dotted ellipses indicate a Glul -high 2c cell-rich population and zone 3–oriented 4c cell-rich population, respectively. In panels with asterisks , some cells were excluded from the analysis because their inclusion reduced the resolution of the expression profiles for the designated genes. Complete mapping of these genes is shown in panel E . ( E ) Complete PCA mapping of 337 hepatocytes for Axin2 , Cyp1a2 , Cyp2c6v1 , Gys2 , Icam1 , and Notch2 .

Article Snippet: Axin2 Rn00577441_m1 , , , , ○ , ○ , , Plays an important role in the regulation of the stability of β-catenin , , , .

Techniques: Gene Expression, Expressing

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Transcriptomic Dissection of Hepatocyte Heterogeneity: Linking Ploidy, Zonation, and Stem/Progenitor Cell Characteristics

doi: 10.1016/j.jcmgh.2019.08.011

Figure Lengend Snippet: Characterization of LPC-like subpopulation in 2c hepatocytes. ( A ) Venn diagram describing the co-expression profile of 3 LPC marker genes: Axin2 , Prom1 , and Lgr5 . ( B ) Cells of Axin2 + Prom1 + Lgr5 + TP population are highlighted in magenta ( upper left ). The ploidy status of TP population cells is indicated in green (2c) and blue (4c) ( lower left ). Expression levels for all of the analyzed LPC marker genes are shown ( right ). In panels with asterisks , some cells were excluded from the analysis because their inclusion reduced the resolution of the expression profiles for the designated genes. Complete mapping of these genes is shown in Figure 8 E . ( C ) Scatter plot with density estimations for Axin2 vs Prom1 and Prom1 vs Lgr5 . Expression levels for each gene are normalized to Actb . ( D ) Scatter plot with density estimations for Axin2 vs Prom1 and Prom1 vs Lgr5 . Expression levels for each gene are normalized to Actb .

Article Snippet: Axin2 Rn00577441_m1 , , , , ○ , ○ , , Plays an important role in the regulation of the stability of β-catenin , , , .

Techniques: Expressing, Marker