Journal: The Journal of comparative neurology

Article Title: Octopamine Immunoreactivity in the Fruit Fly Drosophila melanogaster

doi: 10.1002/cne.903560210

Figure Lengend Snippet: Confocal image of OA-1Ry of type II boutons at the body wall muscles. A: Antihorseradish peroxidase (anti-HRP) staining of ventral body wall muscles showing all classes of boutons. Note that muscle 6 and 7 contain type I boutons only (short arrow) and are devoid of type II boutons. Muscles 12 and 13 contain both type I boutons and type II boutons (long arrow). B: Anti-OA staining showing the same set of muscles as in A (different, sample). Note the correspondence in the distribution of type II boutons (arrow) and OA-IR boutons. C: OA-IRy at “deep” muscles 26, 27, and 29. D: OA-IRy at muscles 12 and 13 in a first-instar larva less than 20 minutes after hatching. Only a couple of boutons (arrow) are formed at this time. TN, transverse nerve. Scale bar = 75 μm in A,B, 70 μm in C, 6 μm in D.

Article Snippet: Washes (six times for 30 minutes each) were followed by an overnight incubation with a horseradish peroxidase (HRP)-conjugated avidin-biotin complex (Vectastain ABC; Vector).

Techniques: Staining

Journal: The Journal of comparative neurology

Article Title: Octopamine Immunoreactivity in the Fruit Fly Drosophila melanogaster

doi: 10.1002/cne.903560210

Figure Lengend Snippet: OA-IRy in the adult brain visualized with an HRP-conjugated secondary antibody. The photomicrographs present horizontal sections of the adult brain at three levels. A: Section of the subesophageal ganglion at the ventral-most level showing some of the SM neurons. B: Section at the esophageal level showing both AL cell clustcrs on each side of esophagus. The small arrow indicates part of the IR fibers detected along the esophageal canal. Note the big fiber bundles (large arrow) along the medial boundary of the optic lobe following the route of the posterior optic tract (POT). C: Section through the superior protocerebrum at a level showing some neurons of the DAC and DPC clusters. AL, antenna1 lobe; DAC, dorsal anterior cluster; DPC, dorsal posterior cluster; SM, subesophageal medial. Scale bar = 30 μm.

Article Snippet: Washes (six times for 30 minutes each) were followed by an overnight incubation with a horseradish peroxidase (HRP)-conjugated avidin-biotin complex (Vectastain ABC; Vector).

Techniques:

Journal: The Journal of comparative neurology

Article Title: Octopamine Immunoreactivity in the Fruit Fly Drosophila melanogaster

doi: 10.1002/cne.903560210

Figure Lengend Snippet: OA-IR cells and processes in the adult brain visualized with an HRP-conjugated secondary antibody. A: Frontal section at the level of the DMC cells. One of the LP cells is also present. B: Horizontal section at the central complex level. Dense IR varicosities are evident in the ellipsoid body (eb). Arrows and arrowhead show the IR fiber bundles detected at this level. Some of the most ventrally located DPC cells are also seen. C: Frontal section at a posterior level showing two major IR fibers (open arrow) crossing each other in the midline. Note some of the DPC cells in different focal plane (solid arrnws) and their positions along the ventrodorsal axis. Arrowheads indicate part or the POT fibers in their posterior route (see also Fig. 7B). DMC, dorsal medial cluster; DPC, dorsal posterior cluster; LP, lateral protocerebrum; E, esophagus; eb, ellipsoid body. Scale bars = 20 μm.

Article Snippet: Washes (six times for 30 minutes each) were followed by an overnight incubation with a horseradish peroxidase (HRP)-conjugated avidin-biotin complex (Vectastain ABC; Vector).

Techniques: