Journal: Developmental Cell

Article Title: Lumen Expansion Facilitates Epiblast-Primitive Endoderm Fate Specification during Mouse Blastocyst Formation

doi: 10.1016/j.devcel.2019.10.011

Figure Lengend Snippet: EPI and PrE Expression Levels Are Reduced in ATP1-Inhibited Embryos (A) Immunofluorescence images of TE (Cdx2), EPI (Sox2), and PrE (Gata4) fate in pre-treatment control (E3.5 WT), Atp1 inhibited (E4.0 500 μM and E4.0 250 μM), and end-stage control (E4.0 DMSO) embryos. Lumen boundaries outlined by dashed white line and mean lumen volume in white text. Scale bars, 10 μm. (B) Boxplot of lumen volume for E3.5 WT (N = 21), E4.0 DMSO (N = 24), E4.0 250 μM Atp1 inhibited (N = 14) and E4.0 500 μM Atp1 inhibited (N = 31) embryos indicating that the impact on lumen volume is concentration dependent. (C) Boxplot of fluorescence levels of Cdx2 (gray), Sox2 (green), and Gata4 (magenta) in E4.0 500 μM Atp1 inhibited embryos compared to E4.0 DMSO controls. (D) Schematic 2D representation of 3D analysis method for spatial segregation of ICM lineages. P 1,2,3,4 are 3D points. L ↔ is a 3D line ( P 1 P 2 ↔ equivalent) that defines the embryonic-abembryonic axis. d ‾ is the 3D line segment ( P 3 P 4 ‾ equivalent) that measures the perpendicular distance from the center of a cell to L ↔ . See Image Analysis for formal definitions of all geometric entities. (E) Boxplot of spatial overlap between EPI and PrE lineages within E4.0 control (DMSO, N = 15), E4.0 Atp1 inhibited (500 μM, N =13) and simulated data of maximal overlap in E4.0 WT embryos (Simulation, N = 27). ∗∗∗∗ p < 0.0001, ∗∗∗ p < 0.001, ∗∗ p < 0.01, ∗ p < 0.05. n.s., not significant. For boxplots: central mark indicates the median; lower edge, 25%; upper edge, 75%; lower whisker, Q1 − (1.5 × IQR), where IQR = Q3 − Q1; upper whisker, Q3 + (1.5 × IQR). See also and ; .

Article Snippet: The following primary antibodies were used in this study: rabbit anti-pERM (Cell Signaling, 3726), mouse anti-Cdx2 (BioGenex, MU392A-UC), goat anti-Sox2 (Santa Cruz Biotechnology, sc-17320), goat anti-Sox2 (R&D Systems, AF2018-SP), rabbit anti-Sox2 (Cell Signaling, 23064), rabbit anti Gata4 (Santa Cruz Biotechnology, sc-9053), goat anti-Gata4 (R&D Systems, AF2606-SP), rabbit anti-GFP (MBL, 598), mouse anti-Rab11 (BD Biosciences; 610656), rat anti-integrin-β1 (Merck Millipore, MAB1997), goat anti-biotinylated Gata4 (R&D Systems, BAF2606) mouse anti-Oct3/4 (Santa Cruz Biotechnology, sc-5279) and mouse anti-Hsp47 (Enzo Life Sciences, M16.10A1).

Techniques: Expressing, Immunofluorescence, Control, Concentration Assay, Fluorescence, Whisker Assay

Journal: Developmental Cell

Article Title: Lumen Expansion Facilitates Epiblast-Primitive Endoderm Fate Specification during Mouse Blastocyst Formation

doi: 10.1016/j.devcel.2019.10.011

Figure Lengend Snippet: PrE Specification and Spatial Segregation of ICM Lineages Is Impaired by Mechanical Inhibition of Lumen Expansion (A) Brightfield images of mechanical deflation. Magenta asterisk marks the needle tip. Dotted magenta line indicates lumen boundary. (B) Immunofluorescence images of EPI (Sox2) and PrE (Gata4) fate in pre-manipulation control (E3.5 WT), E4.0 post-manipulation control (E4.0 WT), and E4.0 mechanically inhibited (E4.0 Mechanical) embryos. Magenta arrowheads indicate the position of cells expressing high levels of Gata4 within the ICM. White dotted line indicates lumen boundaries. Average lumen volume in white text. Scale bars, 10 μm. (C) Boxplot of fluorescence levels of Sox2 (green) and Gata4 (magenta) in mechanically inhibited (Mech., N = 33) and post-manipulation control (WT, N = 28) E4.0 embryos. (D) Boxplot of spatial overlap between EPI and PrE lineages within post-manipulation control (WT, N = 27), mechanically inhibited (Mech., N = 33), E4.0 procedural control (Control, N = 11), and E4.0 simulation of complete overlap in WT conditions (Simulation, N = 27). ∗∗∗∗ p < 0.0001, ∗∗ p < 0.01. n.s., not significant. For boxplots: central mark indicates the median; lower edge, 25%; upper edge, 75%; lower whisker, Q1 − (1.5 × IQR), where IQR = Q3 − Q1; upper whisker, Q3 + (1.5 × IQR). See also Figure S6 and .

Article Snippet: The following primary antibodies were used in this study: rabbit anti-pERM (Cell Signaling, 3726), mouse anti-Cdx2 (BioGenex, MU392A-UC), goat anti-Sox2 (Santa Cruz Biotechnology, sc-17320), goat anti-Sox2 (R&D Systems, AF2018-SP), rabbit anti-Sox2 (Cell Signaling, 23064), rabbit anti Gata4 (Santa Cruz Biotechnology, sc-9053), goat anti-Gata4 (R&D Systems, AF2606-SP), rabbit anti-GFP (MBL, 598), mouse anti-Rab11 (BD Biosciences; 610656), rat anti-integrin-β1 (Merck Millipore, MAB1997), goat anti-biotinylated Gata4 (R&D Systems, BAF2606) mouse anti-Oct3/4 (Santa Cruz Biotechnology, sc-5279) and mouse anti-Hsp47 (Enzo Life Sciences, M16.10A1).

Techniques: Inhibition, Immunofluorescence, Control, Expressing, Fluorescence, Whisker Assay

Journal: Developmental Cell

Article Title: Lumen Expansion Facilitates Epiblast-Primitive Endoderm Fate Specification during Mouse Blastocyst Formation

doi: 10.1016/j.devcel.2019.10.011

Figure Lengend Snippet: Perturbation of FGF4 Signaling in the Lumen Impacts Molecular Specification of EPI and PrE Lineages (A) Immunofluorescence images of EPI (Sox2) and PrE (Gata4) fate in E3.5 post-FGF4 deposition (E3.5 FGF4 Inj.), E3.5 post-PD173074 deposition (E3.5 PD Inj.), and E3.5 post-PBS deposition (E3.5 PBS Inj.). White dotted line indicates lumen boundaries. Average lumen volume in white text. Scale bars, 10 μm. (B) Boxplot of fluorescence levels of Sox2 (green) and Gata4 (magenta) in E3.5 post-FGF4 deposition (FGF4 Inj., N = 24), E3.5 post-PD173074 deposition (PD Inj., N = 21), and E3.5 post-PBS deposition (PBS Inj., N = 13) embryos. (C) Boxplot of luminal volume in E3.5 post-FGF4 deposition (FGF4 Inj., N = 24), E3.5 post-PD173074 deposition (PD Inj., N = 21), and E3.5 post-PBS deposition (PBS Inj., N = 13). ∗∗∗∗ p < 0.0001, ∗∗ p < 0.01. n.s., not significant. For boxplots: central mark indicates the median; lower edge, 25%; upper edge, 75%; lower whisker, Q1 − (1.5 × IQR) where IQR = Q3 − Q1; upper whisker, Q3 + (1.5 × IQR). See also Figure S7 .

Article Snippet: The following primary antibodies were used in this study: rabbit anti-pERM (Cell Signaling, 3726), mouse anti-Cdx2 (BioGenex, MU392A-UC), goat anti-Sox2 (Santa Cruz Biotechnology, sc-17320), goat anti-Sox2 (R&D Systems, AF2018-SP), rabbit anti-Sox2 (Cell Signaling, 23064), rabbit anti Gata4 (Santa Cruz Biotechnology, sc-9053), goat anti-Gata4 (R&D Systems, AF2606-SP), rabbit anti-GFP (MBL, 598), mouse anti-Rab11 (BD Biosciences; 610656), rat anti-integrin-β1 (Merck Millipore, MAB1997), goat anti-biotinylated Gata4 (R&D Systems, BAF2606) mouse anti-Oct3/4 (Santa Cruz Biotechnology, sc-5279) and mouse anti-Hsp47 (Enzo Life Sciences, M16.10A1).

Techniques: Immunofluorescence, Fluorescence, Whisker Assay

Journal: Developmental Cell

Article Title: Lumen Expansion Facilitates Epiblast-Primitive Endoderm Fate Specification during Mouse Blastocyst Formation

doi: 10.1016/j.devcel.2019.10.011

Figure Lengend Snippet: Luminal Deposition of FGF4 Partially Rescues EPI-PrE Specification in ATP1-Inhibited Embryos (A) Immunofluorescence images of EPI (Sox2) and PrE (Gata4) fate in E3.5 post-FGF4 deposition and Atp1 inhibition (E3.5 FGF4 250 μM), E3.5 post-PBS deposition and Atp1 inhibition (E3.5 PBS 250 μM), and E3.5 control embryos (E3.5 DMSO). White dotted line indicates lumen boundaries. Average lumen volume in white text. Magenta arrowhead indicates cell with high Gata4 expression relative to neighboring cells. Scale bars, 10 μm. (B) Boxplot of fluorescence levels of Sox2 (green) and Gata4 (magenta) in E3.5 post-FGF4 deposition and Atp1 inhibition (250 μM FGF4 Inj., N = 42 embryos), E3.5 post-PBS deposition and Atp1 inhibition (250 μM PBS Inj., N = 12), and E3.5 control embryos (DMSO, N = 12). (C) Boxplot of luminal volume in E3.5 post-FGF4 deposition and Atp1 inhibition (250 μM FGF4 Inj., N = 49 embryos), E3.5 post-PBS deposition and Atp1 inhibition (250μM PBS Inj., N = 9), and E3.5 control embryos (DMSO, N = 38). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗∗ p < 0.0001. n.s., not significant. For boxplots: central mark indicates the median; lower edge, 25%; upper edge, 75%; lower whisker, Q1 − (1.5 × IQR), where IQR = Q3 − Q1; upper whisker, Q3 + (1.5 × IQR).

Article Snippet: The following primary antibodies were used in this study: rabbit anti-pERM (Cell Signaling, 3726), mouse anti-Cdx2 (BioGenex, MU392A-UC), goat anti-Sox2 (Santa Cruz Biotechnology, sc-17320), goat anti-Sox2 (R&D Systems, AF2018-SP), rabbit anti-Sox2 (Cell Signaling, 23064), rabbit anti Gata4 (Santa Cruz Biotechnology, sc-9053), goat anti-Gata4 (R&D Systems, AF2606-SP), rabbit anti-GFP (MBL, 598), mouse anti-Rab11 (BD Biosciences; 610656), rat anti-integrin-β1 (Merck Millipore, MAB1997), goat anti-biotinylated Gata4 (R&D Systems, BAF2606) mouse anti-Oct3/4 (Santa Cruz Biotechnology, sc-5279) and mouse anti-Hsp47 (Enzo Life Sciences, M16.10A1).

Techniques: Immunofluorescence, Inhibition, Control, Expressing, Fluorescence, Whisker Assay

Journal: Developmental Cell

Article Title: Lumen Expansion Facilitates Epiblast-Primitive Endoderm Fate Specification during Mouse Blastocyst Formation

doi: 10.1016/j.devcel.2019.10.011

Figure Lengend Snippet:

Article Snippet: The following primary antibodies were used in this study: rabbit anti-pERM (Cell Signaling, 3726), mouse anti-Cdx2 (BioGenex, MU392A-UC), goat anti-Sox2 (Santa Cruz Biotechnology, sc-17320), goat anti-Sox2 (R&D Systems, AF2018-SP), rabbit anti-Sox2 (Cell Signaling, 23064), rabbit anti Gata4 (Santa Cruz Biotechnology, sc-9053), goat anti-Gata4 (R&D Systems, AF2606-SP), rabbit anti-GFP (MBL, 598), mouse anti-Rab11 (BD Biosciences; 610656), rat anti-integrin-β1 (Merck Millipore, MAB1997), goat anti-biotinylated Gata4 (R&D Systems, BAF2606) mouse anti-Oct3/4 (Santa Cruz Biotechnology, sc-5279) and mouse anti-Hsp47 (Enzo Life Sciences, M16.10A1).

Techniques: Recombinant, Software

Journal: Cell reports

Article Title: Neurokinin-1 Receptor Signaling Is Required for Efficient Ca 2+ Flux in T-Cell-Receptor-Activated T Cells

doi: 10.1016/j.celrep.2020.02.054

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Anti-mouse / rat / human Substance P (rabbit polyclonal), Cy3 , Bioss USA , Cat # bs-0064R-Cy3.

Techniques: Functional Assay, Recombinant, Staining, Avidin-Biotin Assay, Blocking Assay, Plasmid Preparation, Inhibition, Fluorescence, SYBR Green Assay, Activation Assay, Bicinchoninic Acid Protein Assay, In Situ, Enzyme-linked Immunosorbent Assay, Activity Assay, Labeling, Software

Journal: Science Advances

Article Title: Promoting the activation of T cells with glycopolymer-modified dendritic cells by enhancing cell interactions

doi: 10.1126/sciadv.abb6595

Figure Lengend Snippet: ( A ) Schematic of HTP transfection with a photo perforation transfection system (PTS) was used. ( B ) Representative images showing HTP transfection. Nuclei were stained by DAPI (blue), and the HA-tagged HTP was stained by FITC-avidin (green). Scale bar, 100 um. ( C ) Quantification of transfection efficiency compared with Lip2000. *** P < 0.001 compared with Lip2000. Data are means ± SEM ( n = 3). ( D ) Schematic of DC modification with glycopolymers. ( E ) Representative images showing green fluorescence on the DC cell surface. Nuclei were stained by DAPI (blue) and biotin-labeled poly-(MAG) (pMB) by FITC-avidin (green). ( F ) Representative images showing the modified DCs incubated in complete medium for specified times (1, 3, and 7 days). ( G ) Viability of engineered DC over the 7-day period. Data are means ± SEM ( n = 3). N.D., not determined.

Article Snippet: Primary antibody hemagglutinin (HA) and FITC-conjugated secondary antibody were from Wuhan Boster Biological Technology Ltd. (Wuhan, China).

Techniques: Transfection, Staining, Avidin-Biotin Assay, Modification, Fluorescence, Labeling, Incubation

Journal: Nanomaterials

Article Title: The Precise Detection of HER-2 Expression in Breast Cancer Cell via Au 25 Probes

doi: 10.3390/nano12060923

Figure Lengend Snippet: Fluorescence imaging of BSA-biotin-fitc labeled HER-2 in SK cells by avidin and primary antibody-biotin at different time points.

Article Snippet: Human HER-2 antibody, HER-2 biotinylated antibody, Avidin and trastuzumab were obtained from Abcam.

Techniques: Fluorescence, Imaging, Labeling, Avidin-Biotin Assay

Journal: Nanomaterials

Article Title: The Precise Detection of HER-2 Expression in Breast Cancer Cell via Au 25 Probes

doi: 10.3390/nano12060923

Figure Lengend Snippet: ( a ) Fluorescence imaging of 12 μM BSA-biotin-Au 25 labeled MDA-MB-231 cells at different time points. ( b ) HER-2 protein expressed in MDA-MB-231 cells before and after paclitaxel stimulation ( c ) Fluorescence imaging of BSA-biotin-Au 25 labeled MDA-MB-231 cells before and after paclitaxel stimulation.

Article Snippet: Human HER-2 antibody, HER-2 biotinylated antibody, Avidin and trastuzumab were obtained from Abcam.

Techniques: Fluorescence, Imaging, Labeling

Journal: Journal of Neuroinflammation

Article Title: Interleukin-1α expression precedes IL-1β after ischemic brain injury and is localised to areas of focal neuronal loss and penumbral tissues

doi: 10.1186/1742-2094-8-186

Figure Lengend Snippet: IL-1α is expressed by microglia localized to focal neuronal and BBB injury 24 h after MCAo . Images are coronal sections from brains of C57BL6/H and CX3CR1-GFP +/- mice 60 min MCAo and 24 h reperfusion. Widefield images show IL-1α-expressing (red), GFP positive (green) microglia in ipsilateral (Ai), not contralateral (ii) amygdala 24 h after MCAo in a CX3CR1-GFP +/- mouse. IL-1α immunohistochemistry with cresyl violet co-staining localises IL-1α expressing microglia to the peri-infarct zone in thalamus (Bi) and cortex (Bii) of a C57BL6/H mouse. Focal IgG staining (red) co-localized with IL-1α positive microglia (green) in the ipsilateral cortex of a C57BL6/H mouse (Ci). No IgG or IL-1α staining detected in the contralateral cortex (Cii). IL-1α positive microglia detected in larger areas of IgG staining in the ipsilateral (Ciii), but not contralateral (Civ) hemisphere. Co-localization of IL-1α positive microglia (red) with areas of neuronal loss (blue) in a C57BL6/H mouse (D). Occasional IL-1α positive microglia also found in areas where neurons were morphologically intact (D, inset). Confocal images (E) are maximum Z projections (Ei, iii) and confocal slices at the level of the nucleus (Eii, iv) of IL-1α expressing, GFP positive microglia in a CX3CR1-GFP +/- mouse. Cells with (Ei, ii), and without (Eiii, iv) nuclear IL-1α. Nuclear fluorescence intensities for IL-1α and GFP were quantified from confocal images, and the fold enrichment of IL-1α and GFP in microglial nuclei was calculated in comparison to whole cell fluorescence (F). All images are representative of n ≥ 3 mice. Quantification is of n = 4 CX3CR1-GFP +/- mouse brains, with each data point representing an individual cell, n ≥ 30 cells per brain.

Article Snippet: Staining of coronal brain sections from C57BL6/H mice (Figure ) and CX3CR1-GFP+/- mice (not shown) 24 h after MCAo for IL-1α and IgG (BA-2000, Vector Labs, biotinylated horse anti-mouse IgG, 2 μg/mL; S-32356, Invitrogen, Alexa 594 conjugated streptavidin, 5 μg/mL) revealed that IL-1α expressing cells co-localized to areas of focal BBB damage, mainly near the penumbral regions of the ipsilateral hemisphere (Figure ).

Techniques: Expressing, Immunohistochemistry, Staining, Fluorescence

Journal: Cell reports

Article Title: Post-Golgi carriers, not lysosomes, confer lysosomal properties to pre-degradative organelles in normal and dystrophic axons

doi: 10.1016/j.celrep.2021.109034

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Subsequent staining steps were performed according to manufacturer’s instructions (Vector Laboratories), which include incubation with biotinylated secondary antibodies, complex formation with avidin and biotinylated HRP using Vectorstain ABC Kit, and reaction with Vector DAB Peroxidase Substrate.

Techniques: Plasmid Preparation, Electron Microscopy, Recombinant, Immunodetection, Fluorescence, Transfection, Expressing, Software

Journal: bioRxiv

Article Title: A Novel Aptamer-Based Approach for Lipoprotein Removal to Achieve Ultra-Pure Blood EV Isolation

doi: 10.1101/2025.03.29.646082

Figure Lengend Snippet: (a) Density vs. size distribution of plasma particles illustrates overlap between lipoproteins (LDL, VLDL, HDL) and EVs under conventional UC and SEC isolation; aptamer-functionalized mesh selectively captures lipoproteins via high-affinity binding; (b) Fluorescence microscopy confirms aptamer immobilization on nylon mesh (green) and specific binding of Cy5-labeled lipoproteins (red); (c) Workflow of sequential capture: (V)LDLs are first bound by ApoB100 aptamers, followed by HDL capture via ApoA1 aptamers, yielding a permeate enriched in EVs free of lipoprotein contamination.

Article Snippet: Following another rinse with distilled water, 100 µL of biotinylated anti-CD63 antibody (BioLegend, San Diego, CA),), biotinylated anti-ApoB100 (R&D Systems, Minneapolis, USA) and biotinylated anti-ApoA1 (R&D Systems, Minneapolis, USA) diluted to 1 µg/mL was added to each microwell for a 1hour reaction.

Techniques: Isolation, Binding Assay, Fluorescence, Microscopy, Labeling

Journal: bioRxiv

Article Title: A Novel Aptamer-Based Approach for Lipoprotein Removal to Achieve Ultra-Pure Blood EV Isolation

doi: 10.1101/2025.03.29.646082

Figure Lengend Snippet: (a–c) Schematics of ApoFilter B (ApoB100-aptamer), ApoFilter A (ApoA1-aptamer), and ApoFilter H (sequential (V)LDL then HDL capture), (d–f) ELISA quantification of lipoprotein levels in input, elution, and permeate fractions, demonstrating >88% capture efficiency and <1% residual lipoprotein in permeates, (g–i) Nanoparticle tracking analysis showing >90% depletion of lipoprotein particles from permeates, (j–l) Total protein measurements confirming >80% reduction in protein content in permeates for all filter types.

Article Snippet: Following another rinse with distilled water, 100 µL of biotinylated anti-CD63 antibody (BioLegend, San Diego, CA),), biotinylated anti-ApoB100 (R&D Systems, Minneapolis, USA) and biotinylated anti-ApoA1 (R&D Systems, Minneapolis, USA) diluted to 1 µg/mL was added to each microwell for a 1hour reaction.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: bioRxiv

Article Title: A Novel Aptamer-Based Approach for Lipoprotein Removal to Achieve Ultra-Pure Blood EV Isolation

doi: 10.1101/2025.03.29.646082

Figure Lengend Snippet: (a) Western blot analysis of lipoprotein markers (ApoA1, ApoB100), EV markers (CD9, CD63), and albumin in samples isolated by ultracentrifugation (UC), size-exclusion chromatography (SEC), or ExoTFF with (+) or without (–) ApoFilter pretreatment, (b–d) ELISA quantification of ApoA1, ApoB100, and CD9 across each isolation workflow, (e) Nanoparticle tracking analysis showing particle concentrations for each method, (f) BCA assay of total protein concentrations for each workflow.

Article Snippet: Following another rinse with distilled water, 100 µL of biotinylated anti-CD63 antibody (BioLegend, San Diego, CA),), biotinylated anti-ApoB100 (R&D Systems, Minneapolis, USA) and biotinylated anti-ApoA1 (R&D Systems, Minneapolis, USA) diluted to 1 µg/mL was added to each microwell for a 1hour reaction.

Techniques: Western Blot, Isolation, Size-exclusion Chromatography, Enzyme-linked Immunosorbent Assay, BIA-KA

Journal: bioRxiv

Article Title: A Novel Aptamer-Based Approach for Lipoprotein Removal to Achieve Ultra-Pure Blood EV Isolation

doi: 10.1101/2025.03.29.646082

Figure Lengend Snippet: (a) UC, (b) ApoFilter→UC, (c) SEC, (d) ApoFilter→SEC, (e) ExoTFF, and (f) ApoFilter→ExoTFF. Integration of ApoFilter markedly increases lipoprotein (ApoA1/ApoB100) and albumin depletion while maintaining or enhancing EV marker recovery across all workflows.

Article Snippet: Following another rinse with distilled water, 100 µL of biotinylated anti-CD63 antibody (BioLegend, San Diego, CA),), biotinylated anti-ApoB100 (R&D Systems, Minneapolis, USA) and biotinylated anti-ApoA1 (R&D Systems, Minneapolis, USA) diluted to 1 µg/mL was added to each microwell for a 1hour reaction.

Techniques: Marker

Journal: Cells

Article Title: Correlative Multi-Modal Microscopy: A Novel Pipeline for Optimizing Fluorescence Microscopy Resolutions in Biological Applications

doi: 10.3390/cells12030354

Figure Lengend Snippet: Correlative Confocal-dSTORM Analysis on Image-Cytometry re-localized cells. ( a ) Representative re-localized G1 cells acquired by the correlative Confocal-STORM protocol described in the text. Confocal Z-Stacks were first acquired of the Tubulin-Atto425 and γH2A.X-Alexa488 fluorescence channel, followed by acquisition of the 53BP1-Cy3 and DynLL1-Alexa Fluor647 channels in dSTORM. The dSTORM plane is identified in the Z-Stack, aligned and merged in the plane (merged image in the second row on the left) and then inserted into the Z-stack (3D projection on the right). ( b ) The described correlative microscopy procedure was applied to cells in the different stages of mitosis, isolated according to the image-cytometry analysis. Z-stacks merged with the dSTORM plane of some representative cells are shown. Scale bar: 5 µm.

Article Snippet: Cells were then rinsed 3 times in PBS and incubated for 1 h with donkey anti-Rat IgG (H + L) biotin (a18749, ThermoFisher Scientific, Waltham, MA, USA) and for 45 min at room temperature with streptavidin Atto425 conjugated (S000-51, Rockland Immunochemicals, Pottstown, PA, USA).

Techniques: Cytometry, Fluorescence, Microscopy, Isolation