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flow cytometer auto40  (Apogee Flow Systems Ltd)
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Apogee Flow Systems Ltd flow cytometer auto40
Gating strategy for the volumetric <t>Auto40</t> flow cytometer (Apogee Flow Systems Ltd, Hemel Hempstead, UK), as depicted from typical figure provided by “Auto-Lymphocyte” software (Apogee Flow Systems Ltd) for the counting of CD4 T lymphocytes in one K 3 -EDTA-anticoagulated blood sample, incubated with phycoerythrin (PE)-conjugated anti-CD4 and PE-Dyomics649-conjugated anti-CD45 monoclonal antibodies (Apogee Flow Systems Ltd): (A) CD45-PE-Dyomics649 × axis-light scatter dot plot scattergram; the primary CD45 gating differentiates clearly the CD45-positive lymphocytes and monocyte populations, and the population of polymorphonuclear cells; (B) CD4-PE × axis-light scatter dot plot scattergram; double secondary CD4 gating allows counting independently the population of CD4-negative lymphocyte and CD4-positive lymphocytes; the CD4 T lymphocytes count in absolute number is given by the CD4-positive gating; the CD4 T lymphocytes count in percentage corresponds to the ratio of CD4-positive lymphocytes count in absolute number to the total CD4-postive and CD4-negative lymphocytes counts, corresponding to the sum of both secondary gating.
Flow Cytometer Auto40, supplied by Apogee Flow Systems Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/flow cytometer auto40/product/Apogee Flow Systems Ltd
Average 90 stars, based on 1 article reviews
flow cytometer auto40 - by Bioz Stars, 2026-06
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Gating strategy for the volumetric Auto40 flow cytometer (Apogee Flow Systems Ltd, Hemel Hempstead, UK), as depicted from typical figure provided by “Auto-Lymphocyte” software (Apogee Flow Systems Ltd) for the counting of CD4 T lymphocytes in one K 3 -EDTA-anticoagulated blood sample, incubated with phycoerythrin (PE)-conjugated anti-CD4 and PE-Dyomics649-conjugated anti-CD45 monoclonal antibodies (Apogee Flow Systems Ltd): (A) CD45-PE-Dyomics649 × axis-light scatter dot plot scattergram; the primary CD45 gating differentiates clearly the CD45-positive lymphocytes and monocyte populations, and the population of polymorphonuclear cells; (B) CD4-PE × axis-light scatter dot plot scattergram; double secondary CD4 gating allows counting independently the population of CD4-negative lymphocyte and CD4-positive lymphocytes; the CD4 T lymphocytes count in absolute number is given by the CD4-positive gating; the CD4 T lymphocytes count in percentage corresponds to the ratio of CD4-positive lymphocytes count in absolute number to the total CD4-postive and CD4-negative lymphocytes counts, corresponding to the sum of both secondary gating.

Journal: BMC Health Services Research

Article Title: Field evaluation in Chad of community usage of CD4 T lymphocyte counting by alternative single-platform flow cytometry

doi: 10.1186/1472-6963-13-373

Figure Lengend Snippet: Gating strategy for the volumetric Auto40 flow cytometer (Apogee Flow Systems Ltd, Hemel Hempstead, UK), as depicted from typical figure provided by “Auto-Lymphocyte” software (Apogee Flow Systems Ltd) for the counting of CD4 T lymphocytes in one K 3 -EDTA-anticoagulated blood sample, incubated with phycoerythrin (PE)-conjugated anti-CD4 and PE-Dyomics649-conjugated anti-CD45 monoclonal antibodies (Apogee Flow Systems Ltd): (A) CD45-PE-Dyomics649 × axis-light scatter dot plot scattergram; the primary CD45 gating differentiates clearly the CD45-positive lymphocytes and monocyte populations, and the population of polymorphonuclear cells; (B) CD4-PE × axis-light scatter dot plot scattergram; double secondary CD4 gating allows counting independently the population of CD4-negative lymphocyte and CD4-positive lymphocytes; the CD4 T lymphocytes count in absolute number is given by the CD4-positive gating; the CD4 T lymphocytes count in percentage corresponds to the ratio of CD4-positive lymphocytes count in absolute number to the total CD4-postive and CD4-negative lymphocytes counts, corresponding to the sum of both secondary gating.

Article Snippet: We herein addressed the principal issues raised by the implementation of the single-platform, volumetric Auto40 flow cytometer (Apogee Flow Systems Ltd, Hemel Hempstead, UK) in 8 community HIV monitoring laboratories of different levels throughout Chad.

Techniques: Flow Cytometry, Software, Incubation, Bioprocessing

Map showing the location of the 22 officially approved health care centers devoted to follow up adult and children patients infected with HIV-1 thorough Chad in 2013 (Am Timane, Abeche, Adre, Ati, Biltine, Bol, Bongor, Doba, Fada, Faya-Largeau, Gos Beïda, Kelo, Koumra, Lere, Maïssala, Mao, Mongo, Moundou, N’Djamena, Ngouri, Pala and Sahr), as well as the 8 centers equipped by the Auto40 flow cytometers (Apogee Flow Systems Ltd) (2 in N’Djamena at the hôpital Militaire D’Instruction, and the Centre D’Appui Psycho-Médico-Social Al-Nadjma; 1 in Mongo, Bongor, Doba, Kelo, Koumra and Maïssala), and the other 9 centers equipped by FACSCount (Becton Dickinson) (2 in N’Djamena; 1 in Abeche, Adre, Gos Beïda, Moundou, Pala and Sahr), or by CyFlow (Partec) (Adre, Biltine, Maïssala, Mao and Ngouri). Note that 5 health care centers for HIV-1-infected patients (Ati; Bol; Fada; Faya-Largeau; Lere) are not yet equipped for CD4 T cell enumeration. The reference laboratory of the hôpital de la Liberté, N’Djamena, is equipped by FACSCalibur (Becton Dickinson). Note that only the health care centers involved in caring HIV-infected patients are shown in the map.

Journal: BMC Health Services Research

Article Title: Field evaluation in Chad of community usage of CD4 T lymphocyte counting by alternative single-platform flow cytometry

doi: 10.1186/1472-6963-13-373

Figure Lengend Snippet: Map showing the location of the 22 officially approved health care centers devoted to follow up adult and children patients infected with HIV-1 thorough Chad in 2013 (Am Timane, Abeche, Adre, Ati, Biltine, Bol, Bongor, Doba, Fada, Faya-Largeau, Gos Beïda, Kelo, Koumra, Lere, Maïssala, Mao, Mongo, Moundou, N’Djamena, Ngouri, Pala and Sahr), as well as the 8 centers equipped by the Auto40 flow cytometers (Apogee Flow Systems Ltd) (2 in N’Djamena at the hôpital Militaire D’Instruction, and the Centre D’Appui Psycho-Médico-Social Al-Nadjma; 1 in Mongo, Bongor, Doba, Kelo, Koumra and Maïssala), and the other 9 centers equipped by FACSCount (Becton Dickinson) (2 in N’Djamena; 1 in Abeche, Adre, Gos Beïda, Moundou, Pala and Sahr), or by CyFlow (Partec) (Adre, Biltine, Maïssala, Mao and Ngouri). Note that 5 health care centers for HIV-1-infected patients (Ati; Bol; Fada; Faya-Largeau; Lere) are not yet equipped for CD4 T cell enumeration. The reference laboratory of the hôpital de la Liberté, N’Djamena, is equipped by FACSCalibur (Becton Dickinson). Note that only the health care centers involved in caring HIV-infected patients are shown in the map.

Article Snippet: We herein addressed the principal issues raised by the implementation of the single-platform, volumetric Auto40 flow cytometer (Apogee Flow Systems Ltd, Hemel Hempstead, UK) in 8 community HIV monitoring laboratories of different levels throughout Chad.

Techniques: Infection

Main characteristics describing the implementation and the 5 years usage (2008–2013) of 8 single-platform, volumetric, Auto40 flow cytometers for community-based point-of-care CD4 T lymphocytes enumeration installed in Chad

Journal: BMC Health Services Research

Article Title: Field evaluation in Chad of community usage of CD4 T lymphocyte counting by alternative single-platform flow cytometry

doi: 10.1186/1472-6963-13-373

Figure Lengend Snippet: Main characteristics describing the implementation and the 5 years usage (2008–2013) of 8 single-platform, volumetric, Auto40 flow cytometers for community-based point-of-care CD4 T lymphocytes enumeration installed in Chad

Article Snippet: We herein addressed the principal issues raised by the implementation of the single-platform, volumetric Auto40 flow cytometer (Apogee Flow Systems Ltd, Hemel Hempstead, UK) in 8 community HIV monitoring laboratories of different levels throughout Chad.

Techniques: Activity Assay, Cell Counting, Control