Journal: Acta Neuropathologica

Article Title: A quantitative Lewy-fold-specific alpha-synuclein seed amplification assay as a progression marker for Parkinson’s disease

doi: 10.1007/s00401-025-02853-y

Figure Lengend Snippet: Comparison of CSF SAA results between PD/DLB and other neurodegenerative entities. PD Parkinson’s disease, DLB dementia with Lewy bodies, CON control, MSA multiple system atrophy, PSP progressive supranuclear palsy, AD Alzheimer’s disease, FTD frontotemporal dementia, RFU relative fluorescence units, AUC area under curve, F max maximum fluorescence, TTT time to threshold, av.: average. a The curve characteristics of individual SAA reactions split for diagnoses are presented for PD/DLB (red, n = 46), MSA (blue, n = 31), PSP (yellow, n = 32), and CON (green, n = 20), highlighting the Lewy-fold specificity of the SAA for PD/DLB cases compared to no SAA reactions in MSA and CON cases that stay negative for 144 h. RFU values are normalized to the maximum intensity of fluorescence of the respective experimental plate. Each curve represents the average of the group ± standard error of the mean. b Comparison of the AUC of each group; each point depicts the median AUC for the replicates of the respective individual. c Comparison of the F max of each group; each point depicts the median F max for the replicates of the respective individual. Statistical analyses were conducted using Wilcoxon tests, resulting in a significance of p < 0.0001 (****) between PD/DLB against the other groups, except for FTD (** p ≤ 0.01)

Article Snippet: Of note, 2 publications compared different SAAs in the same study: Russo et al. [ ] analyzed F max , AUC, TTT, T 50 and endpoint dilution (SD 50 ) across three different laboratories (AbbVie, Amprion and Caughey).

Techniques: Comparison, Control, Fluorescence

Journal: Acta Neuropathologica

Article Title: A quantitative Lewy-fold-specific alpha-synuclein seed amplification assay as a progression marker for Parkinson’s disease

doi: 10.1007/s00401-025-02853-y

Figure Lengend Snippet: Correlation of different SAA parameters with core clinical features

Article Snippet: Of note, 2 publications compared different SAAs in the same study: Russo et al. [ ] analyzed F max , AUC, TTT, T 50 and endpoint dilution (SD 50 ) across three different laboratories (AbbVie, Amprion and Caughey).

Techniques:

Journal: Frontiers in Physiology

Article Title: The effect of combined compression and tactile stimulation on ankle somatosensation in a lunar gravity lower limb load analog

doi: 10.3389/fphys.2025.1537889

Figure Lengend Snippet: Results of ankle AMEDA AUC scores for the whole group. Responses are represented as an AUC score between 0.5 (chance) and 1.0 (perfect score). Somatosensory acuity decreased when on the lunar wedge-bed (simulated lunar gravity, 0.16 g) compared to upright standing (Earth gravity, 1 g) for both barefoot and when wearing the compression-tactile socks. There was no significant difference between barefoot and compression-tactile socks for both body orientations. AMEDA AUC score means are shown with pairwise comparison, where * denotes a significant result. Error bars represent 95% confidence intervals. Results suggest that although somatosensory acuity was reduced on the lunar wedge bed compared to the upright position, the socks were ineffective in improving acuity, thus rejecting our primary hypothesis.

Article Snippet: Group 1 included participants who had an upright standing (Earth gravity, 1 g) barefoot AMEDA AUC score under 0.68 (n = 19) and were considered ‘low’ performers.

Techniques: Comparison

Journal: Frontiers in Physiology

Article Title: The effect of combined compression and tactile stimulation on ankle somatosensation in a lunar gravity lower limb load analog

doi: 10.3389/fphys.2025.1537889

Figure Lengend Snippet: Pairwise comparison results (indicated by p-values shown) of ankle AMEDA AUC scores when analyzed by the performance group. In the low-performer group (left panel), somatosensory acuity was unaffected by the change in body orientation from upright (Earth gravity, 1 g) to lying on the lunar wedge bed (simulated lunar gravity, 0.16 g). Wearing compression-tactile socks improved somatosensory acuity in this group. The medium- and high-performer groups (middle and right panel) did not gain by wearing the compression-tactile socks; however, they were negatively impacted by the change in body orientation. AMEDA AUC score means are shown with pairwise comparison, where * denotes the significant result. Error bars represent 95% confidence intervals. The horizontal reference line at AUC score 0.65 represents a proposed “cut-off,” of where AMEDA scoring under this line is associated with injury (discussed below). Note: a change in scale within the high-performing group (right panel).

Article Snippet: Group 1 included participants who had an upright standing (Earth gravity, 1 g) barefoot AMEDA AUC score under 0.68 (n = 19) and were considered ‘low’ performers.

Techniques: Comparison