Journal: bioRxiv

Article Title: Characterization of cytotrophoblast cell population dynamics throughout pregnancy

doi: 10.1101/2025.01.22.634235

Figure Lengend Snippet: BCAM is a marker for CTBs possessing progenitor capacity within the villous chorion. (A) Immunostaining of BCAM (red) and ITGA6 (green) in term placental tissue. Scale bar, 500 µm and 50 µm (zoom). (B) Immunostaining of BCAM (red) and CGB (green) in term placental tissue. Scale bar, 500 µm. (C) Brightfield images of trophoblast organoid cultures derived from BCAM + cells isolated from term placental tissue at day 7 and day 14. Scale bars, 200 µm. (D) Immunostaining of KRT7 (purple) and GDF15 (yellow) in trophoblast organoids derived from BCAM + cells at day 14. Scale bar, 200 µm.

Article Snippet: Cell suspensions were incubated with 5 μL of anti-BCAM PE-conjugated antibody (Miltenyi Biotech, 130-126-539) in the dark for 10 min at 4°C.

Techniques: Marker, Immunostaining, Derivative Assay, Isolation

Journal: bioRxiv

Article Title: Characterization of cytotrophoblast cell population dynamics throughout pregnancy

doi: 10.1101/2025.01.22.634235

Figure Lengend Snippet: Colocalization of BCAM and LAMA5 suggests contact of progenitor CTBs with basal lamina. (A) Schematic cross section of chorionic villi within the placenta. CTB; cytotrophoblast, STB; syncytiotrophoblast. (B) Electron microscopy images of term placental tissue. Blue arrowheads indicate the syncytium with multiple nuclei, green squares indicate cytotrophoblast, and red arrowheads indicate the basal lamina. Scale bars, 5 µm (top) and 1 µm (bottom). (C) STRING protein network analysis shows top 3 interactors of BCAM with interaction scores. Thickness of lines indicate confidence. (D) Immunostaining of BCAM (red) and LAMA5 (green) in term placental tissue. Scale bar, 500 µm. (E) Immunostaining of BCAM (red) in trophoblast organoids at day 14. Scale bar, 200 µm. Quantification of BCAM intensity profiles measured across multiple line scans (n = 3) per organoid (n = 10). Trend analysis is performed using orthogonal polynomial fitting (dashed line).

Article Snippet: Cell suspensions were incubated with 5 μL of anti-BCAM PE-conjugated antibody (Miltenyi Biotech, 130-126-539) in the dark for 10 min at 4°C.

Techniques: Electron Microscopy, Immunostaining

Journal: bioRxiv

Article Title: Characterization of cytotrophoblast cell population dynamics throughout pregnancy

doi: 10.1101/2025.01.22.634235

Figure Lengend Snippet: Proteomic profiling of decellularized villi across gestation. (A) Ridgeline plot showing average BCAM expression in villous CTB cells throughout gestation. (B) Bar plots showing protein abundance of BCAM and LAMA5 in decellularized villi from first trimester (n = 3) and term (n = 3) placentas. P-values are calculated using a Welch’s t-test. (C) Abundance of basal lamina-associated proteins in decellularized villi from term placentas (n = 3). (D) Venn diagram showing the number of unique or shared proteins in decellularized villi from first trimester and term placentas. Proteins present in all samples (n = 3 each) at either or both gestational times were retained. (E) Vulcano plot showing differential protein abundance of the 2043 proteins shared between decellularized villi from first and term placentas. (F) Fold change of the top 20 differentially abundant proteins in decellularized villi from either first trimester or term placentas. (G) Ridgeline plot showing average HMGA1 expression in villous CTB cells throughout gestation. (H) Bar plot showing protein abundance of HMGA1 in decellularized villi from first trimester (n = 3) and term (n = 3) placentas. P-value is calculated using a Welch’s t-test.

Article Snippet: Cell suspensions were incubated with 5 μL of anti-BCAM PE-conjugated antibody (Miltenyi Biotech, 130-126-539) in the dark for 10 min at 4°C.

Techniques: Expressing, Quantitative Proteomics