Journal: PLoS ONE

Article Title: Novel ATPase Cu 2+ Transporting Beta Polypeptide Mutations in Chinese Families with Wilson's Disease

doi: 10.1371/journal.pone.0066526

Figure Lengend Snippet: Clinical data from the 20 patients with the ATP7B gene mutations.

Article Snippet: The blots were blocked for 1 h in 5% milk in TBST and incubated at 4°C overnight with rabbit anti- ATP7B polyclonal antibody (Novus Biologicals, Littleton, CO, USA), rabbit anti- minichromosome maintenance protein 7 (MCM7) monoclonal antibody (Millipore Biologicals, Billerica, MA, USA), rabbit anti- sterol regulatory element binding protein 1 (SREBP1) polyclonal antibody (Santa Cruz Biotechnology Inc, Santa Cruz, CA, USA), mouse anti- B-cell CLL/lymphoma 2 (BCL2) polyclonal antibody (Santa Cruz Biotechnology Inc, Santa Cruz, CA, USA), mouse anti- BCL2-associated X protein (BAX) polyclonal antibody (Santa Cruz Biotechnology Inc, Santa Cruz, CA, USA) and mouse anti-β-actin polyclonal antibody (Santa Cruz Biotechnology Inc, Santa Cruz, CA, USA).

Techniques:

Journal: PLoS ONE

Article Title: Novel ATPase Cu 2+ Transporting Beta Polypeptide Mutations in Chinese Families with Wilson's Disease

doi: 10.1371/journal.pone.0066526

Figure Lengend Snippet: Statistical differences between ATP7B siRNA transfected groups and AllStars Negative Control siRNA groups are indicated as * ( P <0.01), or untreated group are indicated as # ( P <0.01).

Article Snippet: The blots were blocked for 1 h in 5% milk in TBST and incubated at 4°C overnight with rabbit anti- ATP7B polyclonal antibody (Novus Biologicals, Littleton, CO, USA), rabbit anti- minichromosome maintenance protein 7 (MCM7) monoclonal antibody (Millipore Biologicals, Billerica, MA, USA), rabbit anti- sterol regulatory element binding protein 1 (SREBP1) polyclonal antibody (Santa Cruz Biotechnology Inc, Santa Cruz, CA, USA), mouse anti- B-cell CLL/lymphoma 2 (BCL2) polyclonal antibody (Santa Cruz Biotechnology Inc, Santa Cruz, CA, USA), mouse anti- BCL2-associated X protein (BAX) polyclonal antibody (Santa Cruz Biotechnology Inc, Santa Cruz, CA, USA) and mouse anti-β-actin polyclonal antibody (Santa Cruz Biotechnology Inc, Santa Cruz, CA, USA).

Techniques: Transfection, Negative Control

Journal: PLoS ONE

Article Title: Novel ATPase Cu 2+ Transporting Beta Polypeptide Mutations in Chinese Families with Wilson's Disease

doi: 10.1371/journal.pone.0066526

Figure Lengend Snippet: (A) Western blot analysis of ATP7B , BCL2, BAX, SREBP1 and MCM7 in HepG2 cells following treatment with targeted siRNA and AllStars Negative Control siRNA for 24, 48, and 72 h. β-actin was shown below as loading control. Protein levels of (B) ATP7B , (C) BCL2, (D) BAX, (E) SREBP1 and (F) MCM7 were quantified by densitometry and expression is shown as arbitrary units. Statistical differences for expression of the proteins between ATP7B siRNA transfected groups and AllStars Negative Control siRNA groups are indicated as * ( P <0.05), or ATP7B siRNA untreated group are indicated as # ( P <0.05).

Article Snippet: The blots were blocked for 1 h in 5% milk in TBST and incubated at 4°C overnight with rabbit anti- ATP7B polyclonal antibody (Novus Biologicals, Littleton, CO, USA), rabbit anti- minichromosome maintenance protein 7 (MCM7) monoclonal antibody (Millipore Biologicals, Billerica, MA, USA), rabbit anti- sterol regulatory element binding protein 1 (SREBP1) polyclonal antibody (Santa Cruz Biotechnology Inc, Santa Cruz, CA, USA), mouse anti- B-cell CLL/lymphoma 2 (BCL2) polyclonal antibody (Santa Cruz Biotechnology Inc, Santa Cruz, CA, USA), mouse anti- BCL2-associated X protein (BAX) polyclonal antibody (Santa Cruz Biotechnology Inc, Santa Cruz, CA, USA) and mouse anti-β-actin polyclonal antibody (Santa Cruz Biotechnology Inc, Santa Cruz, CA, USA).

Techniques: Western Blot, Negative Control, Control, Expressing, Transfection

Journal: PLoS ONE

Article Title: Novel ATPase Cu 2+ Transporting Beta Polypeptide Mutations in Chinese Families with Wilson's Disease

doi: 10.1371/journal.pone.0066526

Figure Lengend Snippet: HepG2 cells were stained with 1 μM of Hoechst 33342. Cells were treated with siRNA for 24 h and followed by copper sulfate treatment for 24 h. (A) Treated with 5 nM AllStars Negative Control siRNA. (B) Treated with 5 nM ATP7B siRNA. (C) Treated with 5 nM AllStars Negative Control siRNA+ 50 μM copper sulfate . (D) Treated with 5 nM ATP7B siRNA+ 50 μM copper sulfate. (E) Treated with 5 nM AllStars Negative Control siRNA + 200 μM copper sulfate. (F) Treated with 5 nM ATP7B siRNA+ 200 μM copper sulfate.

Article Snippet: The blots were blocked for 1 h in 5% milk in TBST and incubated at 4°C overnight with rabbit anti- ATP7B polyclonal antibody (Novus Biologicals, Littleton, CO, USA), rabbit anti- minichromosome maintenance protein 7 (MCM7) monoclonal antibody (Millipore Biologicals, Billerica, MA, USA), rabbit anti- sterol regulatory element binding protein 1 (SREBP1) polyclonal antibody (Santa Cruz Biotechnology Inc, Santa Cruz, CA, USA), mouse anti- B-cell CLL/lymphoma 2 (BCL2) polyclonal antibody (Santa Cruz Biotechnology Inc, Santa Cruz, CA, USA), mouse anti- BCL2-associated X protein (BAX) polyclonal antibody (Santa Cruz Biotechnology Inc, Santa Cruz, CA, USA) and mouse anti-β-actin polyclonal antibody (Santa Cruz Biotechnology Inc, Santa Cruz, CA, USA).

Techniques: Staining, Negative Control

Journal: Molecular Pain

Article Title: Differential expression of ATP7A, ATP7B and CTR1 in adult rat dorsal root ganglion tissue

doi: 10.1186/1744-8069-6-53

Figure Lengend Snippet: Copper transporter gene expression in rat tissues determined by quantitative PCR

Article Snippet: The slides were incubated with a chicken anti-ATP7A (1:1000; Abcam), a rabbit anti-ATP7B antibody (NB100-360, Novus Biologicals, Littleton, CO, USA) or a rabbit polyclonal anti-hCTR1 antibody (1:500, Novus Biologicals, Littleton, CO, USA), respectively, at room temperature overnight.

Techniques: Gene Expression

Journal: Molecular Pain

Article Title: Differential expression of ATP7A, ATP7B and CTR1 in adult rat dorsal root ganglion tissue

doi: 10.1186/1744-8069-6-53

Figure Lengend Snippet: ATP7A protein expression in rat DRG tissue . (A) Detection of ATP7A protein by Western blot analysis in DRG of rats aged 4 weeks (lane 1), 12 weeks (lane 2) and 20 weeks (lane 3). Beta actin was probed as a loading control. (B) a and b, neuronal ATP7A immunoreactivity in rat L5 DRG tissue associated with cell bodies of small neurons, intense punctuate vesicular structures in cytoplasm (solid arrows), diffuse cytoplasmic and granular membrane staining (broken arrows), without staining of nuclei (n) or other tissue elements. b was the enlarged frame in a. Inset in a was a negative control. c, Fluorescent immunohistochemistry shows little overlap between ATP7A-immunoreactive (IR) neurons (green), phosphorylated neurofilament heavy subunit (pNF-H)-IR neurons (red), DAPI-stained satellite cells (asterisk) or nerve fibres (f). Scale bar, 20 μm.

Article Snippet: The slides were incubated with a chicken anti-ATP7A (1:1000; Abcam), a rabbit anti-ATP7B antibody (NB100-360, Novus Biologicals, Littleton, CO, USA) or a rabbit polyclonal anti-hCTR1 antibody (1:500, Novus Biologicals, Littleton, CO, USA), respectively, at room temperature overnight.

Techniques: Expressing, Western Blot, Control, Membrane, Staining, Negative Control, Immunohistochemistry

Journal: Scientific Reports

Article Title: Novel and less invasive biomarker assays to measure liver ATP7B in Wilson disease patients

doi: 10.1038/s41598-025-21455-y

Figure Lengend Snippet: ATP7B RNA is detectable in hepatocytes in both fine needle aspiration (FNA) and core needle biopsy (CNB) samples. ( A ) Average relative expression of ATP7B mRNA for each CNB sample was calculated relative to the control samples using TF as the reference gene. ( B ) Average relative expression of ATP7B mRNA for each FNA sample was calculated relative to the average of control samples using TF as the reference gene. Each bar represents the mean of 3 technical replicates. Each dot represents a single technical replicate.

Article Snippet: Anti-ATP7B capture antibody (Abcam, UK, Cat# 131208), anti-ATP7B detection antibody (Novus Biologicals, USA, Cat# NB100-361) and ATP7B recombinant protein (Origene, USA, Cat# TP323635) were purchased from commercial sources.

Techniques: Expressing, Control

Journal: Scientific Reports

Article Title: Novel and less invasive biomarker assays to measure liver ATP7B in Wilson disease patients

doi: 10.1038/s41598-025-21455-y

Figure Lengend Snippet: Ratio of ATP7B mRNA levels in Wilson disease (WD) vs. control samples are correlated in fine needle aspiration biopsies (FNA) and core needle biopsies (CNB). Average relative expression was calculated relative to the mean dCt of control samples for the reference gene TF with 3 technical replicates using the comparative Ct method. R 2 is the coefficient of determination (square of the Pearson correlation coefficient r) and p is the P value in the Pearson correlation.

Article Snippet: Anti-ATP7B capture antibody (Abcam, UK, Cat# 131208), anti-ATP7B detection antibody (Novus Biologicals, USA, Cat# NB100-361) and ATP7B recombinant protein (Origene, USA, Cat# TP323635) were purchased from commercial sources.

Techniques: Control, Expressing

Journal: Scientific Reports

Article Title: Novel and less invasive biomarker assays to measure liver ATP7B in Wilson disease patients

doi: 10.1038/s41598-025-21455-y

Figure Lengend Snippet: ATP7B protein is detected by the immunoassay in core needle biopsies (CNBs) but not detectable in fine needle aspiration biopsies (FNAs). Each bar represents the average of 2 technical replicates. Each point represents a single technical replicate.

Article Snippet: Anti-ATP7B capture antibody (Abcam, UK, Cat# 131208), anti-ATP7B detection antibody (Novus Biologicals, USA, Cat# NB100-361) and ATP7B recombinant protein (Origene, USA, Cat# TP323635) were purchased from commercial sources.

Techniques: