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Image Search Results
Journal: Journal of autoimmunity
Article Title: P2RX7 gene variants associate with altered inflammasome assembly and reduced pyroptosis in chronic nonbacterial osteomyelitis (CNO).
doi: 10.1016/j.jaut.2024.103183
Figure Lengend Snippet: Fig. 2. P2X7 determines pyroptosis and cytokine release. Pyroptosis, IL-1β and IL-18 release in response to priming (LPS) and stimulation (LPS + BzATP) were investigated in P2X7-deficient cells (mono- 3F2 and bi-allelic 4G8 deletion; A-C) and in THP-1 derived 4G8 macrophages reconstituted with wild-type P2X7 expression vectors (D–F). A-C) Kruskal Wallis test and Dunn’s multiple comparison (result shown in black print; A: N ≥6, B/C: N ≥8). A-C) Wilcoxon tests comparing THP1 to 3F2 cells and THP1 to 4G8 cells (result shown in blue print). D-F) Wilcoxon tests (D: N = 6, E/F: N ≥8); *: p < 0.05, **: p < 0.01, ***: p < 0.001, ****: p < 0.001.
Article Snippet: THP-1 cells were treated overnight with PMA (2.5 ng/ml, Sigma- A. Charras et al. Journal of Autoimmunity 144 (2024) 103183 Aldrich) to generate macrophage-like cells, followed by priming (1 μg/ ml LPS, Sigma-Aldrich) for 3h and treated with LPS, LPS/ATP (1 mM, Invivogen),
Techniques: Derivative Assay, Expressing, Comparison