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Bio-Rad
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Bio-Rad
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Bio-Rad
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Bio-Rad
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Active Motif
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Active Motif
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Active Motif
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Illumina Inc
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Epigenomics ag
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10X Genomics
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Illumina Inc
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Image Search Results
Journal: bioRxiv
Article Title: Clonal lineage tracing and parallel multiomics profiling reveal transcriptional diversification induced by ARID1A deficiency
doi: 10.1101/2025.05.24.655549
Figure Lengend Snippet: ( a ) Schematic of the library preparation workflows for PseuMO-Tag–integrated scRNA-seq (top) and scATAC-seq (bottom). scRNA-seq workflow follows the Chromium platform (10x Genomics) and incorporates shPseuMO-Tag transcripts into messenger RNA libraries. The scATAC-seq workflow is based on the ddSEQ platform (Bio-Rad) and enables the integration of shPseuMO-Tag barcodes into chromatin accessibility libraries for clonal tracking. ( b ) Read structures of scRNA-seq and scATAC-seq libraries generated using the PseuMO-Tag system, highlighting barcode positioning and sequencing configurations. ( c ) Schematic of the PseuMO-Tag Decoder pipeline for scRNA-seq and scATAC-seq data. Amplicon-seq reads generated from the respective libraries were processed to associate single-cell barcodes with clonal barcodes (PseuMO-Tags). The resulting matrices link each cell to its clonal identity through either reference-based or reference-free assignment, enabling downstream analyses.
Article Snippet: Single-cell ATAC-seq libraries were generated using the SureCell ATAC-Seq Library Prep Kit and
Techniques: Generated, Sequencing, Amplification
Journal: Scientific Reports
Article Title: scATAC-Seq reveals heterogeneity associated with spermatogonial differentiation in cultured male germline stem cells
doi: 10.1038/s41598-022-25729-7
Figure Lengend Snippet: Profiling chromatin accessibility of germline stem cells (GSCs) after retinoic acid (RA) treatment. ( a ) Schematic of experimental design. The workflow of sample collection after RA treatment and scATAC-seq to measure single nuclei accessibility on the BioRad SureCell ATAC-Seq platform. ( b ) Violin plot of TSS enrichment scores. ( c ) Ridge plot of number of unique fragments. ( d ) Pairwise comparison of gene scores between CTRL and RA-treated samples. The volcano plots show the differential gene score against the − log10(P value) of all investigated genes; each dot represents one gene. Red dots indicate the genes with FDR < 0.05 and log2FC > 0.1 or < -0.1. ( e ) Ridge plots of gene activity of SSC self-renewal and differentiation genes in GSCs with (blue) and without (red) RA treatment.
Article Snippet: The workflow of sample collection after RA treatment and scATAC-seq to measure single nuclei accessibility on the
Techniques: Activity Assay