Journal: Aging

Article Title: Fisetin inhibits the proliferation, migration and invasion of pancreatic cancer by targeting PI3K/AKT/mTOR signaling.

doi: 10.18632/aging.203713

Figure Lengend Snippet: Figure 1. Fisetin inhibits the proliferation of PANC-1 cells. (A) PANC-1 cells were inoculated with Astragaline, Afzelin, Quercetin 3-O-α- L-arabinoside, Narcissoside, Avicularin, Fisetin, Herbacetin, 3'-Hydroxyflavanone and Kaempferol-3-O-rutinoside (50 μM), and the cell index was recorded by RTCA. (B) Histogram exhibiting the repression rate of different compounds. (C) Colony formation assessment of PANC-1 cells inoculated with 0,50 and 100 μM fisetin. (D) Histogram illustrating the number of colonies in each group. (E) Ki67 Immunofluorescence staining of PANC-1 cells inoculated with 0,50 and 100 μM fisetin for 24h. (F) Histogram illustrating the Ki67 positive rate of PANC-1 cells in each group. All assays were replicated thrice, and data are given as means±SD.*p<.05, **p<.01,***p<.001, in contrast with the controls.

Article Snippet: Astragaline (CAS No. : 480-10-4, purity of 99.85%), Afzelin (CAS No. : 482-39-3, purity of 99.62%), Quercetin 3-O-α-L-arabinoside (CAS No. : 22688-79-5, purity of 99.83%), Narcissoside (CAS No. : 604-80-8), Avicularin (CAS No. : 572-30-5), Fisetin (CAS No. : 528-48-3, purity of 98.02%), Herbacetin (CAS No. : 527-95-7, purity > 99.0%) were purchased from MedChemExpress (MCE, Shanghai, China).

Techniques: Immunofluorescence, Staining

Journal: EXCLI Journal

Article Title: Revealing the astragalin mode of anticandidal action

doi: 10.17179/excli2020-2987

Figure Lengend Snippet: a) Inhibition of biofilm formation after treatment with astragalin and ketoconazole, expressed as inhibition percentage (100 % means no biofilm is established). b) Percentage of hyphal cells after 4 h treatment, control is C. albicans without any treatment. c) Nucleotide leakage detected by measuring the absorbance at wavelengths 260 nm and 280 nm after treatment of C. albicans cells with 1½ MIC of astragalin for 0, 15, 30, 45 and 60 min. All values represent means ± SD of three replicates.

Article Snippet: Flavonoid compound astragalin (kaemferol-3O-glucoside) was commercially bought (Extrasynthese, France).

Techniques: Inhibition, Control

Journal: EXCLI Journal

Article Title: Revealing the astragalin mode of anticandidal action

doi: 10.17179/excli2020-2987

Figure Lengend Snippet: Minimal inhibitory concentrations (µg/mL) of astragalin and amphotericin B towards C. albicans in the absence and presence of ergosterol; dissociation constants (K D ) after titration of C. albicans CYP51 (CaCYP51) with tested compounds; cytotoxicity of astragalin towards human gingival fibroblasts (IC 50 μg/mL). NT - not tested

Article Snippet: Flavonoid compound astragalin (kaemferol-3O-glucoside) was commercially bought (Extrasynthese, France).

Techniques: Titration

Journal: EXCLI Journal

Article Title: Revealing the astragalin mode of anticandidal action

doi: 10.17179/excli2020-2987

Figure Lengend Snippet: Expression levels of ERG11 (a) and CDR1 (b) after treatment with MIC of astragalin, ketoconazole and amphotericin B; fluphenazine was used as positive control for CDR1 expression. Values are expressed as Log 2 fold change (log2 FC) of RQ values and presented as an average of two biological replicates.

Article Snippet: Flavonoid compound astragalin (kaemferol-3O-glucoside) was commercially bought (Extrasynthese, France).

Techniques: Expressing, Positive Control

Journal: Journal of Microbiology and Biotechnology

Article Title: Phenolic Profiles of Hardy Kiwifruits and Their Neuroprotective Effects on PC-12 and SH-SY5Y Cells against Oxidative Stress

doi: 10.4014/jmb.2001.01047

Figure Lengend Snippet: Quantification of phenolics and vitamin C in the three cultivars of hardy kiwifruit using reversedphase HPLC.

Article Snippet: Procyanidin B2 and astragalin were purchased from Extrasynthese (Genay, France).

Techniques: Concentration Assay

Journal: Pharmacognosy Magazine

Article Title: Chemometrics-based Approach in Analysis of Arnicae flos

doi: 10.4103/0973-1296.172958

Figure Lengend Snippet: High-performance liquid chromatography chromatograms of: (a) Sample from Vitosha mountain, (b) standard mixture of phenolic compounds at 280 nm. Key to peaks identified: protocatechic (1), chlorogenic (2), caffeic (3), ferulic (4) and p-coumaric (5) acids, and luteolin 7- O -glucoside (6), isoquercitrin (7), apigenin 7- O -glucoside (8), astragalin (9), isorhamnetin 3- O -glucoside (10), quercetin (11), luteolin (12) and kaempferol (13)

Article Snippet: The standards of protocatechuic (1), chlorogenic (2), caffeic (3), ferulic (4) and p -coumaric (5) acids, and luteolin 7- O -glucoside (6), isoquercitrin (7), apigenin 7- O -glucoside (8), astragalin (9), isorhamnetin 3- O -glucoside (10), quercetin (11), luteolin (12), and kaempferol (13) were purchased from Extrasynthese (Genay, France); santonin was supplied by Sigma-Aldrich (St. Louis, USA).

Techniques: High Performance Liquid Chromatography

Journal: Frontiers in Microbiology

Article Title: Robust anti-tubercular profile of Solanum virginianum extract in enhancing isoniazid bioavailability and curtailing stress tolerance in Mycobacterium smegmatis

doi: 10.3389/fmicb.2024.1429027

Figure Lengend Snippet: Identification and quantification of phytochemicals present in SVE using UHPLC.

Article Snippet: Standards of trigonelline (potency: 99.0%, Natural Remedies), protocatechuic acid (potency: 99.5%, Natural Remedies), 4-hydroxy benzoic acid (potency: 99.96%, Sigma Aldrich, USA), vanillic acid (potency: 98.2%, Sigma Aldrich, USA), rutin (potency: 94.2%, Sigma Aldrich, USA), isoquercetin (potency: 98.0%, ChemFaces), astragalin (potency: 98.0%, ChemFaces), neochlorogenic acid (potency: 98.0%, ChemFaces), chlorogenic acid (potency: 98.4%, SRL), and isochlorogenic acid (potency: 98.0%, SRL) were dissolved in methanol to prepare 1,000 ppm primary stock solution, independently.

Techniques: Molecular Weight

Journal: Plants

Article Title: Phytoprofiling of Sorbus L. Inflorescences: A Valuable and Promising Resource for Phenolics

doi: 10.3390/plants11243421

Figure Lengend Snippet: Concentrations of derivatives of the other flavonoids (µg/g DW) in Sorbus inflorescences.

Article Snippet: Analytical and chromatographic grade reagents were used for this study: gradient-grade acetonitrile, MS-grade formic acid, neochlorogenic acid, cryptochlorogenic acid, quercetin 3 -O- (6′′ -O- malonyl)-β-D-glucoside (quercetin 3 -O- malonylglucoside hereinafter), isorhamnetin 3 -O- rutinoside (Sigma-Aldrich GmbH, Steinheim, Germany), HPLC-grade 99.8% trifluoracetic acid, chlorogenic acid, HPLC-grade hyperoside, HPLC-grade isoquercitrin, HPLC-grade rutin, astragalin (Carl Roth GmbH, Karlsruhe, Germany), and 96.3% ethanol (Stumbras SC, Kaunas, Lithuania).

Techniques:

Journal: Plants

Article Title: Phytoprofiling of Sorbus L. Inflorescences: A Valuable and Promising Resource for Phenolics

doi: 10.3390/plants11243421

Figure Lengend Snippet: Dendrogram of hierarchical cluster analysis of Sorbus inflorescence samples according to the phytochemical composition and heatmap of the percentage compositions of identified phenolic compounds: 1—neochlorogenic acid; 2—chlorogenic acid; 3—cryptochlorogenic acid; 4—caffeoylshikimic acid; 5—quercetin dihexoside 1; 6—quercetin dihexoside 2; 7—quercetin pentose hexoside; 8—quercetin dihexoside 3; 9—rutin; 10—hyperoside; 11—isoquercitrin; 12—kaempferol coumaroyl glucoside; 13—quercetin 3 -O- malonylglucoside; 14—isorhamnetin rutinoside; 15—astragalin; 16—dicaffeoylquinic acid derivative 1; 17—sexangularetin derivative; 18—dicaffeoylquinic acid derivative 2; 19—kaempferol acetyl hexoside; 20—dicaffeoylquinic acid derivative 3; 21—isorhamnetin acetyl hexoside.

Article Snippet: Analytical and chromatographic grade reagents were used for this study: gradient-grade acetonitrile, MS-grade formic acid, neochlorogenic acid, cryptochlorogenic acid, quercetin 3 -O- (6′′ -O- malonyl)-β-D-glucoside (quercetin 3 -O- malonylglucoside hereinafter), isorhamnetin 3 -O- rutinoside (Sigma-Aldrich GmbH, Steinheim, Germany), HPLC-grade 99.8% trifluoracetic acid, chlorogenic acid, HPLC-grade hyperoside, HPLC-grade isoquercitrin, HPLC-grade rutin, astragalin (Carl Roth GmbH, Karlsruhe, Germany), and 96.3% ethanol (Stumbras SC, Kaunas, Lithuania).

Techniques:

Journal: Nutrients

Article Title: Identification of a Hydroxygallic Acid Derivative, Zingibroside R1 and a Sterol Lipid as Potential Active Ingredients of Cuscuta chinensis Extract That Has Neuroprotective and Antioxidant Effects in Aged Caenorhabditis elegans

doi: 10.3390/nu14194199

Figure Lengend Snippet: Characterization of bioactive compounds in extracts of the seeds of C. chinensis , its fraction O and the bark of E. ulmoides.

Article Snippet: The fractions enriched in astragalin, pinoresinol, and zingibroside R1 were deposited and stored at AnalytiCon Discovery GmbH under the codes C-3071-I-A06, C-3071-I-A10 and C-3071-L-D03, respectively.

Techniques:

Journal: Nutrients

Article Title: Identification of a Hydroxygallic Acid Derivative, Zingibroside R1 and a Sterol Lipid as Potential Active Ingredients of Cuscuta chinensis Extract That Has Neuroprotective and Antioxidant Effects in Aged Caenorhabditis elegans

doi: 10.3390/nu14194199

Figure Lengend Snippet: The chemical structure of the compounds (astragalin, pinoresinol, and zingibroside R1) from the seeds of C . chinensis .

Article Snippet: The fractions enriched in astragalin, pinoresinol, and zingibroside R1 were deposited and stored at AnalytiCon Discovery GmbH under the codes C-3071-I-A06, C-3071-I-A10 and C-3071-L-D03, respectively.

Techniques:

Journal: Nutrients

Article Title: Identification of a Hydroxygallic Acid Derivative, Zingibroside R1 and a Sterol Lipid as Potential Active Ingredients of Cuscuta chinensis Extract That Has Neuroprotective and Antioxidant Effects in Aged Caenorhabditis elegans

doi: 10.3390/nu14194199

Figure Lengend Snippet: Effect of the C. chinensis extract and its fractions enriched in astragalin, pinoresinol, and zingibroside R1 on the lifespan of C. elegans.

Article Snippet: The fractions enriched in astragalin, pinoresinol, and zingibroside R1 were deposited and stored at AnalytiCon Discovery GmbH under the codes C-3071-I-A06, C-3071-I-A10 and C-3071-L-D03, respectively.

Techniques: Control

Journal: Nutrients

Article Title: Identification of a Hydroxygallic Acid Derivative, Zingibroside R1 and a Sterol Lipid as Potential Active Ingredients of Cuscuta chinensis Extract That Has Neuroprotective and Antioxidant Effects in Aged Caenorhabditis elegans

doi: 10.3390/nu14194199

Figure Lengend Snippet: Effect of the C. chinensis extract and its fractions enriched in astragalin, pinoresinol, and zingibroside R1 on the lifespan of C. elegans . ( A ) Representative survival curves and ( B ) mean lifespan ± SEM from three biological replicates. Significant differences were determined by a log-rank test and subsequent Bonferroni correction with * p < 0.05 or ** p < 0.01.

Article Snippet: The fractions enriched in astragalin, pinoresinol, and zingibroside R1 were deposited and stored at AnalytiCon Discovery GmbH under the codes C-3071-I-A06, C-3071-I-A10 and C-3071-L-D03, respectively.

Techniques:

Journal: Nutrients

Article Title: Identification of a Hydroxygallic Acid Derivative, Zingibroside R1 and a Sterol Lipid as Potential Active Ingredients of Cuscuta chinensis Extract That Has Neuroprotective and Antioxidant Effects in Aged Caenorhabditis elegans

doi: 10.3390/nu14194199

Figure Lengend Snippet: Effect of the C. chinensis extract and its fractions enriched in astragalin, pinoresinol, and zingibroside R1 on the survival of C. elegans after heat stress exposure (37 °C for 3 h) on the 12th day of adulthood.

Article Snippet: The fractions enriched in astragalin, pinoresinol, and zingibroside R1 were deposited and stored at AnalytiCon Discovery GmbH under the codes C-3071-I-A06, C-3071-I-A10 and C-3071-L-D03, respectively.

Techniques: Control

Journal: Nutrients

Article Title: Identification of a Hydroxygallic Acid Derivative, Zingibroside R1 and a Sterol Lipid as Potential Active Ingredients of Cuscuta chinensis Extract That Has Neuroprotective and Antioxidant Effects in Aged Caenorhabditis elegans

doi: 10.3390/nu14194199

Figure Lengend Snippet: Survival of C. elegans treated with C. chinensis and its fractions enriched in astragalin, pinoresinol, and zingibroside R1 after heat stress. ( A ) Representative survival curves of C. elegans following heat stress exposure (37 °C for 3 h) on the 12th day of adulthood and ( B ) mean survival ± SEM after stress exposure from three biological replicates. Significant differences were determined by a log-rank test and Bonferroni correction with * p < 0.05; ** p < 0.01; and **** p < 0.0001.

Article Snippet: The fractions enriched in astragalin, pinoresinol, and zingibroside R1 were deposited and stored at AnalytiCon Discovery GmbH under the codes C-3071-I-A06, C-3071-I-A10 and C-3071-L-D03, respectively.

Techniques:

Journal: Nutrients

Article Title: Identification of a Hydroxygallic Acid Derivative, Zingibroside R1 and a Sterol Lipid as Potential Active Ingredients of Cuscuta chinensis Extract That Has Neuroprotective and Antioxidant Effects in Aged Caenorhabditis elegans

doi: 10.3390/nu14194199

Figure Lengend Snippet: Swim performance of C. elegans after treatment with C. chinensis and its fractions enriched in astragalin, pinoresinol, and zingibroside R1. Body wave number, wave initiation rate, brush stroke, and activity index were determined on the 12th day of adulthood. Error bars are the standard error of the mean (SEM) and one bar represents n ≥ 50 from two independent trials. Statistical significance was determined according to a one-way ANOVA and post-hoc Bonferroni test with * ( p < 0.05) and ** ( p < 0.01).

Article Snippet: The fractions enriched in astragalin, pinoresinol, and zingibroside R1 were deposited and stored at AnalytiCon Discovery GmbH under the codes C-3071-I-A06, C-3071-I-A10 and C-3071-L-D03, respectively.

Techniques: Activity Assay

Journal: Pharmaceuticals

Article Title: Phytochemicals from Passiflora coriacea Juss. Have Anti-Inflammatory and Neuroprotective Effects in Mouse Models

doi: 10.3390/ph17111534

Figure Lengend Snippet: Chemical structure of the compounds isolated from the T3.1 fraction of P. coriacea : isoquercetin (compound 5 ) and astragalin (compound 6 ).

Article Snippet: Conversely, astragalin has been reported to exert a multitarget effect on neuroinflammatory processes, demonstrating that it can improve memory in mice with induced neuroinflammation by suppressing microglial activation [ ].

Techniques: Isolation

Journal: Pharmaceuticals

Article Title: Phytochemicals from Passiflora coriacea Juss. Have Anti-Inflammatory and Neuroprotective Effects in Mouse Models

doi: 10.3390/ph17111534

Figure Lengend Snippet: Inflammatory response, oxidative stress, and antioxidant system of bioactive compounds from P. coriacea . Blue arrows: reactions of the antioxidant system. Brown arrows: lipid peroxidation reactions. Pink dotted line box: RNS production reaction. T2.1: mixture of fitone, 7-dehydrodiosgenin (7DH), and tremulone. T3.1: mixture of isoquercetin and astragalin. SOD: superoxide dismutase; CAT: catalase; GPx: glutathione peroxidase; GR: glutathione reductase; GSH: reduced glutathione; GSSG: oxidized glutathione; O 2 − : superoxide anion radical; iNOS: inducible nitric oxide synthase; 4-HNE: 4-Hydroxynonenal; ACR: acrolein; MDA: malondialdehyde; ROS: reactive oxygen species; and RNS: reactive nitrogen species.

Article Snippet: Conversely, astragalin has been reported to exert a multitarget effect on neuroinflammatory processes, demonstrating that it can improve memory in mice with induced neuroinflammation by suppressing microglial activation [ ].

Techniques: