Journal: Oncotarget

Article Title: Alpha-mangostin induces apoptosis through activation of reactive oxygen species and ASK1/p38 signaling pathway in cervical cancer cells

doi: 10.18632/oncotarget.17659

Figure Lengend Snippet: ( A – C ) HeLa cells were pretreated with or without 50 μM SB203580, a p38 inhibitor, for 2 h, and then treated with or without 20 μM α-mangostin for 24 h. ( D–F ) HeLa cells were transfected with or without the specific siRNA against p38 for 24 h, and then the cells were treated with or without 20 μM α-mangostin for 24 h. After treatment, apoptotic cell death (A, D), mitochondrial membrane potential (B, E), and expressions of indicated apoptosis-related proteins (C, F) were determined. ** P < 0.01.

Article Snippet: The antibodies against phosphory-ERK, phosphory-p38, phosphory-JNK, ERK, p38, JNK, phosphory-MKK3/6, MKK3/6, Bcl-2, Bax, cytochrome C, COX4, α-tubulin, β-actin, siRNA-ERK, siRNA-JNK and siRNA-p38, siRNA-ASK1, siRNA-MKK3/6 were purchased from Santa Cruz Biotechnology (Dallas, Texas).

Techniques: Transfection, Membrane

Journal: Oncotarget

Article Title: Alpha-mangostin induces apoptosis through activation of reactive oxygen species and ASK1/p38 signaling pathway in cervical cancer cells

doi: 10.18632/oncotarget.17659

Figure Lengend Snippet: ( A ) HeLa cells were treated with increased concentrations of α-mangostin (0, 10, 20 and 30 μM) for 24 h. Amounts of phosphorylated and total ASK1 and MKK3/6 were examined by immunoblotting. β-actin is shown as an internal control. ( B – E ) HeLa cells were transfected with or without the specific siRNAs against ASK1 or MKK3/6 for 24 h, and then the cells were treated with or without 20 μM α-mangostin for 24 h. After treatment, expressions of indicated proteins (B), cell viability (C), apoptotic cell death (D), and mitochondrial membrane potential (E) were determined. ** P < 0.01.

Article Snippet: The antibodies against phosphory-ERK, phosphory-p38, phosphory-JNK, ERK, p38, JNK, phosphory-MKK3/6, MKK3/6, Bcl-2, Bax, cytochrome C, COX4, α-tubulin, β-actin, siRNA-ERK, siRNA-JNK and siRNA-p38, siRNA-ASK1, siRNA-MKK3/6 were purchased from Santa Cruz Biotechnology (Dallas, Texas).

Techniques: Western Blot, Control, Transfection, Membrane

Journal: Oncotarget

Article Title: Alpha-mangostin induces apoptosis through activation of reactive oxygen species and ASK1/p38 signaling pathway in cervical cancer cells

doi: 10.18632/oncotarget.17659

Figure Lengend Snippet: Nude mice (BALB/c nu/nu) were treated with DMSO (vehicle) or α-mangostin (20 or 40 mg/kg) every week after an initial subcutaneous injection of HeLa cells. ( A ) Representative tumors isolated from mice at 5 weeks after initiation of treatment are shown. ( B ) Tumor volume at each time interval was measured during treatment. The tumor ( C ) and body ( D ) weight of each mouse were measured at the end of the treatment. Expressions of p-ASK1, p-p38, cleaved-PARP, and cleaved-caspase-3 in the tumor mass were examined by IHC staining ( E ) and by immunoblotting ( F ). Representative results are shown. ** P < 0.01.

Article Snippet: The antibodies against phosphory-ERK, phosphory-p38, phosphory-JNK, ERK, p38, JNK, phosphory-MKK3/6, MKK3/6, Bcl-2, Bax, cytochrome C, COX4, α-tubulin, β-actin, siRNA-ERK, siRNA-JNK and siRNA-p38, siRNA-ASK1, siRNA-MKK3/6 were purchased from Santa Cruz Biotechnology (Dallas, Texas).

Techniques: Injection, Isolation, Immunohistochemistry, Western Blot

Journal: Biology of Reproduction

Article Title: Oxidative stress-induced TGF-beta/TAB1-mediated p38MAPK activation in human amnion epithelial cells †

doi: 10.1093/biolre/ioy135

Figure Lengend Snippet: Antibodies used for the western blot.

Article Snippet: ASK1 , D11C9 , Cell Signaling , 1:750 , Western Blot.

Techniques: Western Blot, Immunofluorescence, Immunoprecipitation