asci Search Results


asci saci hf  (New England Biolabs)
97
New England Biolabs asci saci hf
Asci Saci Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/asci/AscI/pm42009678-325-33-34
Average 97 stars, based on 1 article reviews
asci saci hf - by Bioz Stars, 2026-09
97/100 stars
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px330 rosa26 asci lh291 gu1 lh500  (Addgene inc)
86
Addgene inc px330 rosa26 asci lh291 gu1 lh500
List of All DNA Constructs Used to Produce the Different Second-Generation ifgMosaic Constructs, Related to – (A and B) LoxP - (A) or FRT -containing (B) entry vectors used to clone the desired genes in frame with the upstream FPs and the 2A peptide. (C and D) Map of LoxP - (C) or FRT -containing (D) donor vector used to clone the 3 cassettes from (A) or (B) in the HindIII/NotI sites. (E and F) The Triple ORF donor vectors of C and D can be digested with SgraI/PacI or SgraI/ScaI to insert the mosaic by Cas9 recombineering in pre-modified ES cells (E) or by ligation to <t>Rosa26</t> gene targeting vectors (F). Plasmids <t>LH500</t> or LH416 are required to express and guide the Cas9 to the pre-modified (E) or wildtype (F) Rosa26 locus. (G) The large SgraI/PacI fragments generated in C and D can be inserted by recombineering in different acceptor Rosa26 BACs containing the following promoters: CAG, Tre-Tight, or UAS 4x NR (4 UAS elements non-repeated). BAC G256 can be used for transgenesis in zebrafish and contains a marker to directly select transgenic founders (Cmcl2-turquoise) based on turquoise fluorescence in the heart. (H) Smaller vectors that can be used to directly clone the mosaic constructs downstream of the Tre-Tight or UAS promoters for titratable and reversible induction. These vectors can also be digested with the rare cutters SgraI/PacI and cloned in a plasmid (AG103) containing the Rosa26 homology arms for gene targeting. LOXP1, LoxN ; LOXP2, Lox2272 ; LOXP3, LoxP ; FRT1, F3 ; FRT2, 5T2 ; FRT3, 545 ; 2A, viral peptide allowing equimolar expression of multiple independent proteins from a single ORF; Mb2, second generation membrane tag; HA, V5 and His (small epitopes that can be used for specific antibody detection); H2B, histone tag that targets proteins to the chromatin/nucleus; WPRE, Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element that enhances gene expression; Sv40pA, polyadenylation signal to stop transcription; N-PhiM, non-fluorescent protein that is used as a reporter of promoter expression; CAG, Strong and ubiquitous promoter; PGK-Neo, resistance marker for ES cell selection; INS-INS, Double Chicken B-globin insulator to increase gene expression and minimize regulatory interference.
Px330 Rosa26 Asci Lh291 Gu1 Lh500, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/asci/px330+Rosa26+AscI+LH291Gu1+(LH500)+(Plasmid+%2399628)/pmc06381294-102-0-10
Average 86 stars, based on 1 article reviews
px330 rosa26 asci lh291 gu1 lh500 - by Bioz Stars, 2026-09
86/100 stars
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strain background m tuberculosis erdman strain bei  (Addgene inc)
90
Addgene inc strain background m tuberculosis erdman strain bei
List of All DNA Constructs Used to Produce the Different Second-Generation ifgMosaic Constructs, Related to – (A and B) LoxP - (A) or FRT -containing (B) entry vectors used to clone the desired genes in frame with the upstream FPs and the 2A peptide. (C and D) Map of LoxP - (C) or FRT -containing (D) donor vector used to clone the 3 cassettes from (A) or (B) in the HindIII/NotI sites. (E and F) The Triple ORF donor vectors of C and D can be digested with SgraI/PacI or SgraI/ScaI to insert the mosaic by Cas9 recombineering in pre-modified ES cells (E) or by ligation to <t>Rosa26</t> gene targeting vectors (F). Plasmids <t>LH500</t> or LH416 are required to express and guide the Cas9 to the pre-modified (E) or wildtype (F) Rosa26 locus. (G) The large SgraI/PacI fragments generated in C and D can be inserted by recombineering in different acceptor Rosa26 BACs containing the following promoters: CAG, Tre-Tight, or UAS 4x NR (4 UAS elements non-repeated). BAC G256 can be used for transgenesis in zebrafish and contains a marker to directly select transgenic founders (Cmcl2-turquoise) based on turquoise fluorescence in the heart. (H) Smaller vectors that can be used to directly clone the mosaic constructs downstream of the Tre-Tight or UAS promoters for titratable and reversible induction. These vectors can also be digested with the rare cutters SgraI/PacI and cloned in a plasmid (AG103) containing the Rosa26 homology arms for gene targeting. LOXP1, LoxN ; LOXP2, Lox2272 ; LOXP3, LoxP ; FRT1, F3 ; FRT2, 5T2 ; FRT3, 545 ; 2A, viral peptide allowing equimolar expression of multiple independent proteins from a single ORF; Mb2, second generation membrane tag; HA, V5 and His (small epitopes that can be used for specific antibody detection); H2B, histone tag that targets proteins to the chromatin/nucleus; WPRE, Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element that enhances gene expression; Sv40pA, polyadenylation signal to stop transcription; N-PhiM, non-fluorescent protein that is used as a reporter of promoter expression; CAG, Strong and ubiquitous promoter; PGK-Neo, resistance marker for ES cell selection; INS-INS, Double Chicken B-globin insulator to increase gene expression and minimize regulatory interference.
Strain Background M Tuberculosis Erdman Strain Bei, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/asci/pBlue-hFX-HA(AscI)+(Plasmid+%2315404)/10__7554_slash_elife__45957-115-65-62
Average 90 stars, based on 1 article reviews
strain background m tuberculosis erdman strain bei - by Bioz Stars, 2026-09
90/100 stars
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ppgk1 luciferase  (Addgene inc)
93
Addgene inc ppgk1 luciferase
List of All DNA Constructs Used to Produce the Different Second-Generation ifgMosaic Constructs, Related to – (A and B) LoxP - (A) or FRT -containing (B) entry vectors used to clone the desired genes in frame with the upstream FPs and the 2A peptide. (C and D) Map of LoxP - (C) or FRT -containing (D) donor vector used to clone the 3 cassettes from (A) or (B) in the HindIII/NotI sites. (E and F) The Triple ORF donor vectors of C and D can be digested with SgraI/PacI or SgraI/ScaI to insert the mosaic by Cas9 recombineering in pre-modified ES cells (E) or by ligation to <t>Rosa26</t> gene targeting vectors (F). Plasmids <t>LH500</t> or LH416 are required to express and guide the Cas9 to the pre-modified (E) or wildtype (F) Rosa26 locus. (G) The large SgraI/PacI fragments generated in C and D can be inserted by recombineering in different acceptor Rosa26 BACs containing the following promoters: CAG, Tre-Tight, or UAS 4x NR (4 UAS elements non-repeated). BAC G256 can be used for transgenesis in zebrafish and contains a marker to directly select transgenic founders (Cmcl2-turquoise) based on turquoise fluorescence in the heart. (H) Smaller vectors that can be used to directly clone the mosaic constructs downstream of the Tre-Tight or UAS promoters for titratable and reversible induction. These vectors can also be digested with the rare cutters SgraI/PacI and cloned in a plasmid (AG103) containing the Rosa26 homology arms for gene targeting. LOXP1, LoxN ; LOXP2, Lox2272 ; LOXP3, LoxP ; FRT1, F3 ; FRT2, 5T2 ; FRT3, 545 ; 2A, viral peptide allowing equimolar expression of multiple independent proteins from a single ORF; Mb2, second generation membrane tag; HA, V5 and His (small epitopes that can be used for specific antibody detection); H2B, histone tag that targets proteins to the chromatin/nucleus; WPRE, Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element that enhances gene expression; Sv40pA, polyadenylation signal to stop transcription; N-PhiM, non-fluorescent protein that is used as a reporter of promoter expression; CAG, Strong and ubiquitous promoter; PGK-Neo, resistance marker for ES cell selection; INS-INS, Double Chicken B-globin insulator to increase gene expression and minimize regulatory interference.
Ppgk1 Luciferase, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/asci/pPGK1-(AscI)-%CE%BBN-T2A-SpHIS5+(pNTI347)+(Plasmid+%23115429)/pmc04641015-90-16-24
Average 93 stars, based on 1 article reviews
ppgk1 luciferase - by Bioz Stars, 2026-09
93/100 stars
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asci  (New England Biolabs)
97
New England Biolabs asci
List of All DNA Constructs Used to Produce the Different Second-Generation ifgMosaic Constructs, Related to – (A and B) LoxP - (A) or FRT -containing (B) entry vectors used to clone the desired genes in frame with the upstream FPs and the 2A peptide. (C and D) Map of LoxP - (C) or FRT -containing (D) donor vector used to clone the 3 cassettes from (A) or (B) in the HindIII/NotI sites. (E and F) The Triple ORF donor vectors of C and D can be digested with SgraI/PacI or SgraI/ScaI to insert the mosaic by Cas9 recombineering in pre-modified ES cells (E) or by ligation to <t>Rosa26</t> gene targeting vectors (F). Plasmids <t>LH500</t> or LH416 are required to express and guide the Cas9 to the pre-modified (E) or wildtype (F) Rosa26 locus. (G) The large SgraI/PacI fragments generated in C and D can be inserted by recombineering in different acceptor Rosa26 BACs containing the following promoters: CAG, Tre-Tight, or UAS 4x NR (4 UAS elements non-repeated). BAC G256 can be used for transgenesis in zebrafish and contains a marker to directly select transgenic founders (Cmcl2-turquoise) based on turquoise fluorescence in the heart. (H) Smaller vectors that can be used to directly clone the mosaic constructs downstream of the Tre-Tight or UAS promoters for titratable and reversible induction. These vectors can also be digested with the rare cutters SgraI/PacI and cloned in a plasmid (AG103) containing the Rosa26 homology arms for gene targeting. LOXP1, LoxN ; LOXP2, Lox2272 ; LOXP3, LoxP ; FRT1, F3 ; FRT2, 5T2 ; FRT3, 545 ; 2A, viral peptide allowing equimolar expression of multiple independent proteins from a single ORF; Mb2, second generation membrane tag; HA, V5 and His (small epitopes that can be used for specific antibody detection); H2B, histone tag that targets proteins to the chromatin/nucleus; WPRE, Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element that enhances gene expression; Sv40pA, polyadenylation signal to stop transcription; N-PhiM, non-fluorescent protein that is used as a reporter of promoter expression; CAG, Strong and ubiquitous promoter; PGK-Neo, resistance marker for ES cell selection; INS-INS, Double Chicken B-globin insulator to increase gene expression and minimize regulatory interference.
Asci, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/asci/AscI/us11118187-1431-9-17
Average 97 stars, based on 1 article reviews
asci - by Bioz Stars, 2026-09
97/100 stars
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sgrna targeting mata ho cut site pcas kanmx prg645 plexo asci tcyc1 pact1 lexa er b112 tcyc1 prg205mx leu2mx  (Addgene inc)
88
Addgene inc sgrna targeting mata ho cut site pcas kanmx prg645 plexo asci tcyc1 pact1 lexa er b112 tcyc1 prg205mx leu2mx
List of All DNA Constructs Used to Produce the Different Second-Generation ifgMosaic Constructs, Related to – (A and B) LoxP - (A) or FRT -containing (B) entry vectors used to clone the desired genes in frame with the upstream FPs and the 2A peptide. (C and D) Map of LoxP - (C) or FRT -containing (D) donor vector used to clone the 3 cassettes from (A) or (B) in the HindIII/NotI sites. (E and F) The Triple ORF donor vectors of C and D can be digested with SgraI/PacI or SgraI/ScaI to insert the mosaic by Cas9 recombineering in pre-modified ES cells (E) or by ligation to <t>Rosa26</t> gene targeting vectors (F). Plasmids <t>LH500</t> or LH416 are required to express and guide the Cas9 to the pre-modified (E) or wildtype (F) Rosa26 locus. (G) The large SgraI/PacI fragments generated in C and D can be inserted by recombineering in different acceptor Rosa26 BACs containing the following promoters: CAG, Tre-Tight, or UAS 4x NR (4 UAS elements non-repeated). BAC G256 can be used for transgenesis in zebrafish and contains a marker to directly select transgenic founders (Cmcl2-turquoise) based on turquoise fluorescence in the heart. (H) Smaller vectors that can be used to directly clone the mosaic constructs downstream of the Tre-Tight or UAS promoters for titratable and reversible induction. These vectors can also be digested with the rare cutters SgraI/PacI and cloned in a plasmid (AG103) containing the Rosa26 homology arms for gene targeting. LOXP1, LoxN ; LOXP2, Lox2272 ; LOXP3, LoxP ; FRT1, F3 ; FRT2, 5T2 ; FRT3, 545 ; 2A, viral peptide allowing equimolar expression of multiple independent proteins from a single ORF; Mb2, second generation membrane tag; HA, V5 and His (small epitopes that can be used for specific antibody detection); H2B, histone tag that targets proteins to the chromatin/nucleus; WPRE, Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element that enhances gene expression; Sv40pA, polyadenylation signal to stop transcription; N-PhiM, non-fluorescent protein that is used as a reporter of promoter expression; CAG, Strong and ubiquitous promoter; PGK-Neo, resistance marker for ES cell selection; INS-INS, Double Chicken B-globin insulator to increase gene expression and minimize regulatory interference.
Sgrna Targeting Mata Ho Cut Site Pcas Kanmx Prg645 Plexo Asci Tcyc1 Pact1 Lexa Er B112 Tcyc1 Prg205mx Leu2mx, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/asci/pRG645_lexO-AscI_LexA-TF_LEU2MX+(Plasmid+%23154813)/pmc07672422__gkaa833_supplemental_file-52-8-4
Average 88 stars, based on 1 article reviews
sgrna targeting mata ho cut site pcas kanmx prg645 plexo asci tcyc1 pact1 lexa er b112 tcyc1 prg205mx leu2mx - by Bioz Stars, 2026-09
88/100 stars
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asci file format  (Esri inc)
90
Esri inc asci file format
List of All DNA Constructs Used to Produce the Different Second-Generation ifgMosaic Constructs, Related to – (A and B) LoxP - (A) or FRT -containing (B) entry vectors used to clone the desired genes in frame with the upstream FPs and the 2A peptide. (C and D) Map of LoxP - (C) or FRT -containing (D) donor vector used to clone the 3 cassettes from (A) or (B) in the HindIII/NotI sites. (E and F) The Triple ORF donor vectors of C and D can be digested with SgraI/PacI or SgraI/ScaI to insert the mosaic by Cas9 recombineering in pre-modified ES cells (E) or by ligation to <t>Rosa26</t> gene targeting vectors (F). Plasmids <t>LH500</t> or LH416 are required to express and guide the Cas9 to the pre-modified (E) or wildtype (F) Rosa26 locus. (G) The large SgraI/PacI fragments generated in C and D can be inserted by recombineering in different acceptor Rosa26 BACs containing the following promoters: CAG, Tre-Tight, or UAS 4x NR (4 UAS elements non-repeated). BAC G256 can be used for transgenesis in zebrafish and contains a marker to directly select transgenic founders (Cmcl2-turquoise) based on turquoise fluorescence in the heart. (H) Smaller vectors that can be used to directly clone the mosaic constructs downstream of the Tre-Tight or UAS promoters for titratable and reversible induction. These vectors can also be digested with the rare cutters SgraI/PacI and cloned in a plasmid (AG103) containing the Rosa26 homology arms for gene targeting. LOXP1, LoxN ; LOXP2, Lox2272 ; LOXP3, LoxP ; FRT1, F3 ; FRT2, 5T2 ; FRT3, 545 ; 2A, viral peptide allowing equimolar expression of multiple independent proteins from a single ORF; Mb2, second generation membrane tag; HA, V5 and His (small epitopes that can be used for specific antibody detection); H2B, histone tag that targets proteins to the chromatin/nucleus; WPRE, Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element that enhances gene expression; Sv40pA, polyadenylation signal to stop transcription; N-PhiM, non-fluorescent protein that is used as a reporter of promoter expression; CAG, Strong and ubiquitous promoter; PGK-Neo, resistance marker for ES cell selection; INS-INS, Double Chicken B-globin insulator to increase gene expression and minimize regulatory interference.
Asci File Format, supplied by Esri inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/asci/asci+file+format/10__4314_slash_sajas__v46i4__12-64-4-12
Average 90 stars, based on 1 article reviews
asci file format - by Bioz Stars, 2026-09
90/100 stars
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asci macrorestriction pattern ncimb 701851  (NCIMB Ltd)
90
NCIMB Ltd asci macrorestriction pattern ncimb 701851
List of All DNA Constructs Used to Produce the Different Second-Generation ifgMosaic Constructs, Related to – (A and B) LoxP - (A) or FRT -containing (B) entry vectors used to clone the desired genes in frame with the upstream FPs and the 2A peptide. (C and D) Map of LoxP - (C) or FRT -containing (D) donor vector used to clone the 3 cassettes from (A) or (B) in the HindIII/NotI sites. (E and F) The Triple ORF donor vectors of C and D can be digested with SgraI/PacI or SgraI/ScaI to insert the mosaic by Cas9 recombineering in pre-modified ES cells (E) or by ligation to <t>Rosa26</t> gene targeting vectors (F). Plasmids <t>LH500</t> or LH416 are required to express and guide the Cas9 to the pre-modified (E) or wildtype (F) Rosa26 locus. (G) The large SgraI/PacI fragments generated in C and D can be inserted by recombineering in different acceptor Rosa26 BACs containing the following promoters: CAG, Tre-Tight, or UAS 4x NR (4 UAS elements non-repeated). BAC G256 can be used for transgenesis in zebrafish and contains a marker to directly select transgenic founders (Cmcl2-turquoise) based on turquoise fluorescence in the heart. (H) Smaller vectors that can be used to directly clone the mosaic constructs downstream of the Tre-Tight or UAS promoters for titratable and reversible induction. These vectors can also be digested with the rare cutters SgraI/PacI and cloned in a plasmid (AG103) containing the Rosa26 homology arms for gene targeting. LOXP1, LoxN ; LOXP2, Lox2272 ; LOXP3, LoxP ; FRT1, F3 ; FRT2, 5T2 ; FRT3, 545 ; 2A, viral peptide allowing equimolar expression of multiple independent proteins from a single ORF; Mb2, second generation membrane tag; HA, V5 and His (small epitopes that can be used for specific antibody detection); H2B, histone tag that targets proteins to the chromatin/nucleus; WPRE, Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element that enhances gene expression; Sv40pA, polyadenylation signal to stop transcription; N-PhiM, non-fluorescent protein that is used as a reporter of promoter expression; CAG, Strong and ubiquitous promoter; PGK-Neo, resistance marker for ES cell selection; INS-INS, Double Chicken B-globin insulator to increase gene expression and minimize regulatory interference.
Asci Macrorestriction Pattern Ncimb 701851, supplied by NCIMB Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/asci/asci+macrorestriction+pattern+ncimb+701851/10__1128_slash_aem__68__2__765___771__2002-151-5-5
Average 90 stars, based on 1 article reviews
asci macrorestriction pattern ncimb 701851 - by Bioz Stars, 2026-09
90/100 stars
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bsiwi-t2agal4dbd_asci  (GenScript corporation)
90
GenScript corporation bsiwi-t2agal4dbd_asci
List of All DNA Constructs Used to Produce the Different Second-Generation ifgMosaic Constructs, Related to – (A and B) LoxP - (A) or FRT -containing (B) entry vectors used to clone the desired genes in frame with the upstream FPs and the 2A peptide. (C and D) Map of LoxP - (C) or FRT -containing (D) donor vector used to clone the 3 cassettes from (A) or (B) in the HindIII/NotI sites. (E and F) The Triple ORF donor vectors of C and D can be digested with SgraI/PacI or SgraI/ScaI to insert the mosaic by Cas9 recombineering in pre-modified ES cells (E) or by ligation to <t>Rosa26</t> gene targeting vectors (F). Plasmids <t>LH500</t> or LH416 are required to express and guide the Cas9 to the pre-modified (E) or wildtype (F) Rosa26 locus. (G) The large SgraI/PacI fragments generated in C and D can be inserted by recombineering in different acceptor Rosa26 BACs containing the following promoters: CAG, Tre-Tight, or UAS 4x NR (4 UAS elements non-repeated). BAC G256 can be used for transgenesis in zebrafish and contains a marker to directly select transgenic founders (Cmcl2-turquoise) based on turquoise fluorescence in the heart. (H) Smaller vectors that can be used to directly clone the mosaic constructs downstream of the Tre-Tight or UAS promoters for titratable and reversible induction. These vectors can also be digested with the rare cutters SgraI/PacI and cloned in a plasmid (AG103) containing the Rosa26 homology arms for gene targeting. LOXP1, LoxN ; LOXP2, Lox2272 ; LOXP3, LoxP ; FRT1, F3 ; FRT2, 5T2 ; FRT3, 545 ; 2A, viral peptide allowing equimolar expression of multiple independent proteins from a single ORF; Mb2, second generation membrane tag; HA, V5 and His (small epitopes that can be used for specific antibody detection); H2B, histone tag that targets proteins to the chromatin/nucleus; WPRE, Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element that enhances gene expression; Sv40pA, polyadenylation signal to stop transcription; N-PhiM, non-fluorescent protein that is used as a reporter of promoter expression; CAG, Strong and ubiquitous promoter; PGK-Neo, resistance marker for ES cell selection; INS-INS, Double Chicken B-globin insulator to increase gene expression and minimize regulatory interference.
Bsiwi T2agal4dbd Asci, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/asci/bsiwi+t2agal4dbd+asci/pmc10410749__pnas__2307451120__sapp-15-12-14
Average 90 stars, based on 1 article reviews
bsiwi-t2agal4dbd_asci - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
bsiwi-t2a-ad_asci  (GenScript corporation)
90
GenScript corporation bsiwi-t2a-ad_asci
List of All DNA Constructs Used to Produce the Different Second-Generation ifgMosaic Constructs, Related to – (A and B) LoxP - (A) or FRT -containing (B) entry vectors used to clone the desired genes in frame with the upstream FPs and the 2A peptide. (C and D) Map of LoxP - (C) or FRT -containing (D) donor vector used to clone the 3 cassettes from (A) or (B) in the HindIII/NotI sites. (E and F) The Triple ORF donor vectors of C and D can be digested with SgraI/PacI or SgraI/ScaI to insert the mosaic by Cas9 recombineering in pre-modified ES cells (E) or by ligation to <t>Rosa26</t> gene targeting vectors (F). Plasmids <t>LH500</t> or LH416 are required to express and guide the Cas9 to the pre-modified (E) or wildtype (F) Rosa26 locus. (G) The large SgraI/PacI fragments generated in C and D can be inserted by recombineering in different acceptor Rosa26 BACs containing the following promoters: CAG, Tre-Tight, or UAS 4x NR (4 UAS elements non-repeated). BAC G256 can be used for transgenesis in zebrafish and contains a marker to directly select transgenic founders (Cmcl2-turquoise) based on turquoise fluorescence in the heart. (H) Smaller vectors that can be used to directly clone the mosaic constructs downstream of the Tre-Tight or UAS promoters for titratable and reversible induction. These vectors can also be digested with the rare cutters SgraI/PacI and cloned in a plasmid (AG103) containing the Rosa26 homology arms for gene targeting. LOXP1, LoxN ; LOXP2, Lox2272 ; LOXP3, LoxP ; FRT1, F3 ; FRT2, 5T2 ; FRT3, 545 ; 2A, viral peptide allowing equimolar expression of multiple independent proteins from a single ORF; Mb2, second generation membrane tag; HA, V5 and His (small epitopes that can be used for specific antibody detection); H2B, histone tag that targets proteins to the chromatin/nucleus; WPRE, Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element that enhances gene expression; Sv40pA, polyadenylation signal to stop transcription; N-PhiM, non-fluorescent protein that is used as a reporter of promoter expression; CAG, Strong and ubiquitous promoter; PGK-Neo, resistance marker for ES cell selection; INS-INS, Double Chicken B-globin insulator to increase gene expression and minimize regulatory interference.
Bsiwi T2a Ad Asci, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/asci/bsiwi+t2a+ad+asci/pmc10410749__pnas__2307451120__sapp-17-12-14
Average 90 stars, based on 1 article reviews
bsiwi-t2a-ad_asci - by Bioz Stars, 2026-09
90/100 stars
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modified mrna vaccine encoding five antigens including ny-eso-1 asci, mage-a3/as15  (CureVac Inc)
90
CureVac Inc modified mrna vaccine encoding five antigens including ny-eso-1 asci, mage-a3/as15
List of All DNA Constructs Used to Produce the Different Second-Generation ifgMosaic Constructs, Related to – (A and B) LoxP - (A) or FRT -containing (B) entry vectors used to clone the desired genes in frame with the upstream FPs and the 2A peptide. (C and D) Map of LoxP - (C) or FRT -containing (D) donor vector used to clone the 3 cassettes from (A) or (B) in the HindIII/NotI sites. (E and F) The Triple ORF donor vectors of C and D can be digested with SgraI/PacI or SgraI/ScaI to insert the mosaic by Cas9 recombineering in pre-modified ES cells (E) or by ligation to <t>Rosa26</t> gene targeting vectors (F). Plasmids <t>LH500</t> or LH416 are required to express and guide the Cas9 to the pre-modified (E) or wildtype (F) Rosa26 locus. (G) The large SgraI/PacI fragments generated in C and D can be inserted by recombineering in different acceptor Rosa26 BACs containing the following promoters: CAG, Tre-Tight, or UAS 4x NR (4 UAS elements non-repeated). BAC G256 can be used for transgenesis in zebrafish and contains a marker to directly select transgenic founders (Cmcl2-turquoise) based on turquoise fluorescence in the heart. (H) Smaller vectors that can be used to directly clone the mosaic constructs downstream of the Tre-Tight or UAS promoters for titratable and reversible induction. These vectors can also be digested with the rare cutters SgraI/PacI and cloned in a plasmid (AG103) containing the Rosa26 homology arms for gene targeting. LOXP1, LoxN ; LOXP2, Lox2272 ; LOXP3, LoxP ; FRT1, F3 ; FRT2, 5T2 ; FRT3, 545 ; 2A, viral peptide allowing equimolar expression of multiple independent proteins from a single ORF; Mb2, second generation membrane tag; HA, V5 and His (small epitopes that can be used for specific antibody detection); H2B, histone tag that targets proteins to the chromatin/nucleus; WPRE, Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element that enhances gene expression; Sv40pA, polyadenylation signal to stop transcription; N-PhiM, non-fluorescent protein that is used as a reporter of promoter expression; CAG, Strong and ubiquitous promoter; PGK-Neo, resistance marker for ES cell selection; INS-INS, Double Chicken B-globin insulator to increase gene expression and minimize regulatory interference.
Modified Mrna Vaccine Encoding Five Antigens Including Ny Eso 1 Asci, Mage A3/As15, supplied by CureVac Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/asci/modified+mrna+vaccine+encoding+five+antigens+including+ny+eso+1+asci++mage+a3+as15/pmc07148596-137-12-14
Average 90 stars, based on 1 article reviews
modified mrna vaccine encoding five antigens including ny-eso-1 asci, mage-a3/as15 - by Bioz Stars, 2026-09
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asci program  (Lawrence Livermore National Security LLC)
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Lawrence Livermore National Security LLC asci program
List of All DNA Constructs Used to Produce the Different Second-Generation ifgMosaic Constructs, Related to – (A and B) LoxP - (A) or FRT -containing (B) entry vectors used to clone the desired genes in frame with the upstream FPs and the 2A peptide. (C and D) Map of LoxP - (C) or FRT -containing (D) donor vector used to clone the 3 cassettes from (A) or (B) in the HindIII/NotI sites. (E and F) The Triple ORF donor vectors of C and D can be digested with SgraI/PacI or SgraI/ScaI to insert the mosaic by Cas9 recombineering in pre-modified ES cells (E) or by ligation to <t>Rosa26</t> gene targeting vectors (F). Plasmids <t>LH500</t> or LH416 are required to express and guide the Cas9 to the pre-modified (E) or wildtype (F) Rosa26 locus. (G) The large SgraI/PacI fragments generated in C and D can be inserted by recombineering in different acceptor Rosa26 BACs containing the following promoters: CAG, Tre-Tight, or UAS 4x NR (4 UAS elements non-repeated). BAC G256 can be used for transgenesis in zebrafish and contains a marker to directly select transgenic founders (Cmcl2-turquoise) based on turquoise fluorescence in the heart. (H) Smaller vectors that can be used to directly clone the mosaic constructs downstream of the Tre-Tight or UAS promoters for titratable and reversible induction. These vectors can also be digested with the rare cutters SgraI/PacI and cloned in a plasmid (AG103) containing the Rosa26 homology arms for gene targeting. LOXP1, LoxN ; LOXP2, Lox2272 ; LOXP3, LoxP ; FRT1, F3 ; FRT2, 5T2 ; FRT3, 545 ; 2A, viral peptide allowing equimolar expression of multiple independent proteins from a single ORF; Mb2, second generation membrane tag; HA, V5 and His (small epitopes that can be used for specific antibody detection); H2B, histone tag that targets proteins to the chromatin/nucleus; WPRE, Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element that enhances gene expression; Sv40pA, polyadenylation signal to stop transcription; N-PhiM, non-fluorescent protein that is used as a reporter of promoter expression; CAG, Strong and ubiquitous promoter; PGK-Neo, resistance marker for ES cell selection; INS-INS, Double Chicken B-globin insulator to increase gene expression and minimize regulatory interference.
Asci Program, supplied by Lawrence Livermore National Security LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/asci/asci+white/10__1016_slash_j__infsof__2015__07__011-313-5-10
Average 90 stars, based on 1 article reviews
asci program - by Bioz Stars, 2026-09
90/100 stars
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List of All DNA Constructs Used to Produce the Different Second-Generation ifgMosaic Constructs, Related to – (A and B) LoxP - (A) or FRT -containing (B) entry vectors used to clone the desired genes in frame with the upstream FPs and the 2A peptide. (C and D) Map of LoxP - (C) or FRT -containing (D) donor vector used to clone the 3 cassettes from (A) or (B) in the HindIII/NotI sites. (E and F) The Triple ORF donor vectors of C and D can be digested with SgraI/PacI or SgraI/ScaI to insert the mosaic by Cas9 recombineering in pre-modified ES cells (E) or by ligation to Rosa26 gene targeting vectors (F). Plasmids LH500 or LH416 are required to express and guide the Cas9 to the pre-modified (E) or wildtype (F) Rosa26 locus. (G) The large SgraI/PacI fragments generated in C and D can be inserted by recombineering in different acceptor Rosa26 BACs containing the following promoters: CAG, Tre-Tight, or UAS 4x NR (4 UAS elements non-repeated). BAC G256 can be used for transgenesis in zebrafish and contains a marker to directly select transgenic founders (Cmcl2-turquoise) based on turquoise fluorescence in the heart. (H) Smaller vectors that can be used to directly clone the mosaic constructs downstream of the Tre-Tight or UAS promoters for titratable and reversible induction. These vectors can also be digested with the rare cutters SgraI/PacI and cloned in a plasmid (AG103) containing the Rosa26 homology arms for gene targeting. LOXP1, LoxN ; LOXP2, Lox2272 ; LOXP3, LoxP ; FRT1, F3 ; FRT2, 5T2 ; FRT3, 545 ; 2A, viral peptide allowing equimolar expression of multiple independent proteins from a single ORF; Mb2, second generation membrane tag; HA, V5 and His (small epitopes that can be used for specific antibody detection); H2B, histone tag that targets proteins to the chromatin/nucleus; WPRE, Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element that enhances gene expression; Sv40pA, polyadenylation signal to stop transcription; N-PhiM, non-fluorescent protein that is used as a reporter of promoter expression; CAG, Strong and ubiquitous promoter; PGK-Neo, resistance marker for ES cell selection; INS-INS, Double Chicken B-globin insulator to increase gene expression and minimize regulatory interference.

Journal: Cell

Article Title: Dual ifgMosaic: A Versatile Method for Multispectral and Combinatorial Mosaic Gene-Function Analysis

doi: 10.1016/j.cell.2017.07.031

Figure Lengend Snippet: List of All DNA Constructs Used to Produce the Different Second-Generation ifgMosaic Constructs, Related to – (A and B) LoxP - (A) or FRT -containing (B) entry vectors used to clone the desired genes in frame with the upstream FPs and the 2A peptide. (C and D) Map of LoxP - (C) or FRT -containing (D) donor vector used to clone the 3 cassettes from (A) or (B) in the HindIII/NotI sites. (E and F) The Triple ORF donor vectors of C and D can be digested with SgraI/PacI or SgraI/ScaI to insert the mosaic by Cas9 recombineering in pre-modified ES cells (E) or by ligation to Rosa26 gene targeting vectors (F). Plasmids LH500 or LH416 are required to express and guide the Cas9 to the pre-modified (E) or wildtype (F) Rosa26 locus. (G) The large SgraI/PacI fragments generated in C and D can be inserted by recombineering in different acceptor Rosa26 BACs containing the following promoters: CAG, Tre-Tight, or UAS 4x NR (4 UAS elements non-repeated). BAC G256 can be used for transgenesis in zebrafish and contains a marker to directly select transgenic founders (Cmcl2-turquoise) based on turquoise fluorescence in the heart. (H) Smaller vectors that can be used to directly clone the mosaic constructs downstream of the Tre-Tight or UAS promoters for titratable and reversible induction. These vectors can also be digested with the rare cutters SgraI/PacI and cloned in a plasmid (AG103) containing the Rosa26 homology arms for gene targeting. LOXP1, LoxN ; LOXP2, Lox2272 ; LOXP3, LoxP ; FRT1, F3 ; FRT2, 5T2 ; FRT3, 545 ; 2A, viral peptide allowing equimolar expression of multiple independent proteins from a single ORF; Mb2, second generation membrane tag; HA, V5 and His (small epitopes that can be used for specific antibody detection); H2B, histone tag that targets proteins to the chromatin/nucleus; WPRE, Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element that enhances gene expression; Sv40pA, polyadenylation signal to stop transcription; N-PhiM, non-fluorescent protein that is used as a reporter of promoter expression; CAG, Strong and ubiquitous promoter; PGK-Neo, resistance marker for ES cell selection; INS-INS, Double Chicken B-globin insulator to increase gene expression and minimize regulatory interference.

Article Snippet: pX330 Rosa26 AscI LH291 Gu1 (LH500) , This paper, , Addgene #99628.

Techniques: Construct, Plasmid Preparation, Modification, Ligation, Generated, Marker, Transgenic Assay, Fluorescence, Clone Assay, Expressing, Membrane, Virus, Gene Expression, Selection

Journal: Cell

Article Title: Dual ifgMosaic: A Versatile Method for Multispectral and Combinatorial Mosaic Gene-Function Analysis

doi: 10.1016/j.cell.2017.07.031

Figure Lengend Snippet:

Article Snippet: pX330 Rosa26 AscI LH291 Gu1 (LH500) , This paper, , Addgene #99628.

Techniques: Plasmid Preparation, Recombinant, Imaging, Gene Expression, DNA Synthesis, Software