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Summary of molecular profiling analyses from various CLIA certified methods in a patient with malignant phyllodes sarcoma
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Summary of molecular profiling analyses from various CLIA certified methods in a patient with malignant phyllodes sarcoma
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Summary of molecular profiling analyses from various CLIA certified methods in a patient with malignant phyllodes sarcoma
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Image Search Results


Summary of molecular profiling analyses from various CLIA certified methods in a patient with malignant phyllodes sarcoma

Journal: Orphanet Journal of Rare Diseases

Article Title: Comprehensive characterization of malignant phyllodes tumor by whole genomic and proteomic analysis: biological implications for targeted therapy opportunities

doi: 10.1186/1750-1172-8-112

Figure Lengend Snippet: Summary of molecular profiling analyses from various CLIA certified methods in a patient with malignant phyllodes sarcoma

Article Snippet: Whole-genome array-based comparative genomic hybridization (array CGH) was performed by using the CLIA-certified DNAarrayTM (CombiMatrix Diagnostics, Irvine, CA).

Techniques: Sequencing, Hybridization, Immunohistochemistry, Staining

Imaging studies, histopathology and FISH studies: UA and B: CT of the chest showing metastatic masses in the lung. C Hematoxylin and Eosin stained section of the tumor from the right mastectomy shows a malignant sarcoma/malignant phyllodes tumor that has high stromal cellularity and a mitotic index of 11 per 10 high power fields. D HER - 2 / neu by Fluorescence in Situ Hybridization (FISH) HER-2 gene amplification was assessed utilizing the PathVysion assay (Vysis Corp., Downers Grove, Illinois). The identification probes for the HER-2 (SpectrumOrange) and alpha satellite DNA sequence at the centromeric region of chromosome 17 (SpectrumGreen) were hybridized according to the manufacturer’s guidelines. At least twenty non-overlapping nuclei containing at least one orange and one green signal were enumerated. The ratio of orange signals (HER-2 gene) to green signals (chromosome 17) was calculated. A ratio greater than or equal to 2.0 is considered as amplified based on the FDA approval in this kit. The CAP HER-2 consensus conference 2002 suggested that a ratio of 1.8-2.2 be considered as borderline.

Journal: Orphanet Journal of Rare Diseases

Article Title: Comprehensive characterization of malignant phyllodes tumor by whole genomic and proteomic analysis: biological implications for targeted therapy opportunities

doi: 10.1186/1750-1172-8-112

Figure Lengend Snippet: Imaging studies, histopathology and FISH studies: UA and B: CT of the chest showing metastatic masses in the lung. C Hematoxylin and Eosin stained section of the tumor from the right mastectomy shows a malignant sarcoma/malignant phyllodes tumor that has high stromal cellularity and a mitotic index of 11 per 10 high power fields. D HER - 2 / neu by Fluorescence in Situ Hybridization (FISH) HER-2 gene amplification was assessed utilizing the PathVysion assay (Vysis Corp., Downers Grove, Illinois). The identification probes for the HER-2 (SpectrumOrange) and alpha satellite DNA sequence at the centromeric region of chromosome 17 (SpectrumGreen) were hybridized according to the manufacturer’s guidelines. At least twenty non-overlapping nuclei containing at least one orange and one green signal were enumerated. The ratio of orange signals (HER-2 gene) to green signals (chromosome 17) was calculated. A ratio greater than or equal to 2.0 is considered as amplified based on the FDA approval in this kit. The CAP HER-2 consensus conference 2002 suggested that a ratio of 1.8-2.2 be considered as borderline.

Article Snippet: Whole-genome array-based comparative genomic hybridization (array CGH) was performed by using the CLIA-certified DNAarrayTM (CombiMatrix Diagnostics, Irvine, CA).

Techniques: Imaging, Histopathology, Staining, Fluorescence, In Situ Hybridization, Amplification, Sequencing