Journal: Scientific Reports

Article Title: Organophosphate pesticide DEDT promotes diabetic retinopathy progression via AMPK/Nrf2/HO-1 pathway

doi: 10.1038/s41598-026-37183-w

Figure Lengend Snippet: DEDT impairs retinal cell viability and disrupts tight junction integrity. (A-B) The effects of various concentrations of DEDT (0–50 µM) on cell viability under high glucose conditions were assessed in ( A ) HRMECs and ( B ) ARPE-19 cells. Data are presented as mean ± SD ( n = 8). (C-D) The time-dependent effects of 40 µM DEDT on cell viability under high glucose exposure were evaluated in ( C ) HRMECs and ( D ) ARPE-19 cells. ( E ) Transendothelial electrical resistance (TEER) was used to evaluate the barrier integrity of HRMECs treated with 40 µM DEDT under high glucose (HG) conditions at various time points. ( F ) Dextran permeability assay. (G-J) Protein expression levels of tight junction markers ZO-1, occludin, and claudin-5 were measured using WB analysis. All DEDT treatments were conducted under HG exposure. TEER values were expressed in Ω·cm². Data are presented as mean ± SD. Statistical significance was determined by one-way ANOVA followed by Tukey’s post-hoc test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns = not significant compared to the DR or HG group.

Article Snippet: HRMEC cells was purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China; Catalog No. CP-H130) and ARPE-19 cells was purchased from ATCC (Catalog No. CRL-2302).

Techniques: FITC-Dextran Permeability Assay, Expressing

Journal: Frontiers in Medicine

Article Title: Efavirenz treatment improves retinal vaso-obliteration and pathological neovascularization in a mouse model of retinopathy of prematurity

doi: 10.3389/fmed.2026.1745351

Figure Lengend Snippet: (A) Western blot analyses were performed using protein isolated from human retinal astrocytes (HRA), human brain microglia (HMC3), human retinal endothelial cells (HREC), human retinal pigment epithelial cells (ARPE), and differentiated retinal neuronal cells (RNC). Immunofluorescence staining of retinal cross sections from each experimental group to evaluate (B) GFAP and (C) RBPMS expression at P17. (D) IBA-1 immunostaining in representative retinal flat mount images from P17 mice of each group to identify quiescent (red arrowheads) or activated (yellow arrowheads) retinal microglia. (E–G) Western blot analyses of GFAP, RBPMS, VCAM-1, and ICAM-1 expression. β-Actin was used as a loading control. Representative data presented in this figure are derived from 3–6 retinas per group, each obtained from a different mouse.

Article Snippet: Human retinal pigment epithelial cells (ARPE-19; ATCC® CRL-2302TM) were maintained in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin.

Techniques: Western Blot, Isolation, Immunofluorescence, Staining, Expressing, Immunostaining, Control, Derivative Assay

Journal: Nature Communications

Article Title: Development of graphitic carbon nitride quantum dots-based oxygen self-sufficient platforms for enhanced corneal crosslinking

doi: 10.1038/s41467-024-49645-8

Figure Lengend Snippet: a Cell viability of HCEC cells incubated with g-C 3 N 4 QDs at different concentrations (biologically independent samples, two-way ANOVA multiple comparison test, mean ± SD, n = 6, the absence of a P -value indicates no statistical difference between the groups ( p > 0.05)). b Cell viability of hRMEC cells incubated with g-C 3 N 4 QDs at different concentrations (biologically independent samples, two-way ANOVA multiple comparison test, mean ± SD, n = 6, the absence of a P -value indicates no statistical difference between the groups ( p > 0.05)). c Cell viability of ARPE−19 cells incubated with g-C 3 N 4 QDs at different concentrations (biologically independent samples, two-way ANOVA multiple comparison test, mean ± SD, n = 6, the absence of a P -value indicates no statistical difference between the groups ( p > 0.05)). d Calcein-AM/PI double staining of HCEC cells incubated with g-C 3 N 4 QDs at different concentrations (The experiment was repeated three times independently with similar results). e , f Flow-cytometric analysis of HCEC cells incubated with g-C 3 N 4 QDs at different concentrations. (biologically independent samples, two-way ANOVA multiple comparison test, mean ± SD, n = 3, living cell (0 vs 100, p < 0.0001; 0 vs 200, p < 0.0001; 0 vs 400, p < 0.0001; 100 vs 400, p < 0.0001; 200 vs 400, p < 0.0001), Early apoptotic cell (0 vs 100, p < 0.0001; 0 vs 200, p < 0.0001; 0 vs 400, p < 0.0001); Late Apoptotic Cell (0 vs 100, p = 0.0027; 0 vs 200, p < 0.0001; 0 vs 400, p < 0.0001; 100 vs 200, p = 0.0044; 100 vs 400, p < 0.0001; 200 vs 400, p < 0.0001), Necrosis Cell (0 vs 400, p = 0.0019; 200 vs 400, p = 0.0009), the absence of a P -value indicates no statistical difference between the groups ( p > 0.05)).

Article Snippet: The human retinal microvascular endothelial cells (HRMEC), human retinal epithelial cells (ARPE-19), human corneal epithelial cells (HCEC) were provided by the Global Bioresource Center.

Techniques: Incubation, Comparison, Double Staining

Journal: Frontiers in Cell and Developmental Biology

Article Title: SLC7A11 Reduces Laser-Induced Choroidal Neovascularization by Inhibiting RPE Ferroptosis and VEGF Production

doi: 10.3389/fcell.2021.639851

Figure Lengend Snippet: The cell viability and lipid peroxidation level of SLC7A11 KD ARPE19. (A–C) The protein expression of SLC7A11 and VEGFA in ARPE19 with different concentration of H 2 O 2 , including 0, 200, 400 μM ( n = 3). (D,E) The mRNA level of SLC7A11 and VEGFA in ARPE19 with different concentration of H 2 O 2 ( n = 6). (F–I) The expression of SLC7A11, GPX4, and NRF2 protein using shRNA to knock down SLC7A11 ( n = 3). (J,K) The mRNA of SLC7A11 and NRF2 using shRNA to knock down SLC7A11 ( n = 6). (L) The concentration of VEGF of SLC7A11 KD ARPE19 by ELISA ( n = 5). (M) The level of lipid peroxidation of SLC7A11 KD ARPE19 by BODIPY TM 581/591 ( n = 5). (N,O) The cell viability of SLC7A11 KD ARPE19 with the treatment of Fer-1 (1000 nM) and Lip-1 (20 μM) ( n = 6). Bar graphs show mean ± SD. P -values: T-test and one-way ANOVA analysis. (*** p < 0.001, ** p < 0.01, * p < 0.05). Scale bar = 50 μm.

Article Snippet: As SAS concentration increased, ARPE19 induced ferroptosis by reducing intracellular GSH levels.

Techniques: Expressing, Concentration Assay, shRNA, Knockdown, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Cell and Developmental Biology

Article Title: SLC7A11 Reduces Laser-Induced Choroidal Neovascularization by Inhibiting RPE Ferroptosis and VEGF Production

doi: 10.3389/fcell.2021.639851

Figure Lengend Snippet: The effect of SLC7A11 inhibitor on ARPE cell line. (A) The GSH of ARPE19 with different concentration of SAS ( n = 5). (B) The concentration of VEGF of ARPE19 with SAS stimulation by ELISA ( n = 5). (C,D) The protein expression of GPX4 with different concentration of SAS ( n = 3). (E) The cell viability of ARPE19 with different concentration of SAS ( n = 5). (F,G) The cell viability of ARPE19 with treatment of Fer-1 (1000 nM) or Lip-1 (20 μM) after stimulation of 1600 μM SAS ( n = 6). (H–J) The level of lipid peroxidation under SAS stimulation by BODIPY TM 581/591 ( n = 5). Bar graphs show mean ± SD. P -values: one-way ANOVA analysis and t -test (*** p < 0.001, ** p < 0.01, * p < 0.05). Scale bar = 50 μm.

Article Snippet: As SAS concentration increased, ARPE19 induced ferroptosis by reducing intracellular GSH levels.

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Expressing

Journal: Frontiers in Cell and Developmental Biology

Article Title: SLC7A11 Reduces Laser-Induced Choroidal Neovascularization by Inhibiting RPE Ferroptosis and VEGF Production

doi: 10.3389/fcell.2021.639851

Figure Lengend Snippet: The cell viability of SLC7A11 OE ARPE19. (A–C) SLC7A11 and NRF2 protein in SLC7A11 OE ARPE19 were evaluated by Western blot ( n = 3). (D–F) SLC7A11 , NRF2 , and VEGFA mRNA in SLC7A11 OE ARPE19 were evaluated by RT-PCR ( n = 6). (G,H) The cell viability of SLC7A11 OE ARPE19 with the stimulation of erastin (200 μM) or RSL3 (20 μM) ( n = 5). (I) The cell viability of anti-VEGF treatment and SLC7A11 OE ARPE19 with the stimulation of erastin (200 μM) or RSL3 (20 μM) ( n = 5). Bar graphs show mean ± SD. P -values: one-way ANOVA analysis and t -test (*** p < 0.001, ** p < 0.01, * p < 0.05).

Article Snippet: As SAS concentration increased, ARPE19 induced ferroptosis by reducing intracellular GSH levels.

Techniques: Western Blot, Reverse Transcription Polymerase Chain Reaction