Journal: Experimental and Therapeutic Medicine

Article Title: PDK1 inhibition reduces autophagy and cell senescence through the PI3K/AKT signalling pathway in a cigarette smoke mouse emphysema model

doi: 10.3892/etm.2023.11922

Figure Lengend Snippet: Expression of p16 protein in the airway epithelial tissues. (A) Immunofluorescence staining for the analysis of p16 protein expression in airway epithelial tissues. Magnification, x400. (B) Comparison of p16 protein expression among the experimental groups. aP<0.05 vs. control. bP<0.05 vs. emphysema. CS + CSE, emphysema group; CS3, PI3K inhibitor group; CS1, PDK1 inhibitor group; PDK1, phosphoinositide dependent protein kinase 1; p16, cyclin-dependent kinase inhibitor 2A.

Article Snippet: Slices were incubated with anti-LC3B (1:100; cat. no. APG8B; Abcepta) and anti-p16 antibodies (1:100; cat. no. Bs-11652R; BIOSS) overnight at 4˚C.

Techniques: Expressing, Immunofluorescence, Staining, Comparison, Control

Journal: Experimental and Therapeutic Medicine

Article Title: PDK1 inhibition reduces autophagy and cell senescence through the PI3K/AKT signalling pathway in a cigarette smoke mouse emphysema model

doi: 10.3892/etm.2023.11922

Figure Lengend Snippet: PI3K, PDK1, AKT, LC3B II and p16 protein expression levels. Western blotting for (A) PI3K, (B) PDK1, (C) AKT, (D) LC3B and (E) p16 expression in the airway epithelial tissues. GAPDH as an internal control. Semi-quantification of PI3K, PDK1, AKT, LC3B and p16 protein expression in airway epithelial tissues in each group. aP<0.05 vs. control. bP<0.05 vs. emphysema. The protein expression levels were expressed as the ratio of band intensity for the target protein relative to that for the internal control GAPDH. Values are expressed as the mean ± standard deviation. CS + CSE, emphysema group; CS3, PI3K inhibitor group; CS1, PDK1 inhibitor group; PDK1, phosphoinositide dependent protein kinase 1; p16, cyclin-dependent kinase inhibitor 2A.

Article Snippet: Slices were incubated with anti-LC3B (1:100; cat. no. APG8B; Abcepta) and anti-p16 antibodies (1:100; cat. no. Bs-11652R; BIOSS) overnight at 4˚C.

Techniques: Expressing, Western Blot, Control, Standard Deviation

Journal: BMC cancer

Article Title: ARPC5 acts as a potential prognostic biomarker that is associated with cell proliferation, migration and immune infiltrate in gliomas.

doi: 10.1186/s12885-023-11433-w

Figure Lengend Snippet: Fig. 1 Abnormally high expression of ARPC5 in glioma. (A) Differential expression of ARPC5 in various tumor types was analyzed based on the TIMER online website. (B) The distinct upregulation of ARPC5 in glioma was demonstrated in GEPIA online website. (C) Box plot based on the expression of ARPC5 in the GSE2223 (Glioma = 50, Normal = 4). (D) Box plot based on the expression of ARPC5 in the GSE29796 (Glioma = 52, Normal = 20). (E) Box plot based on the expression of ARPC5 in the GSE116520 (Glioma = 34, Normal = 8). (F) Box plot based on the protein expression of ARPC5 in the CPTAC samples (Glioma = 99, Normal = 10). (G) ARPC5 protein expression was detected in cerebral cortex (Patient id: 1582), low grade glioma (Patient id: 3365), and high-grade glioma (Patient id: 3251) tissues from HPA dataset. (H) Representative images of ARPC5 expression in glioma and its surrounding tissues. *p < 0.05, **p < 0.01, ***p < 0.001

Article Snippet: Glioma tissue samples and glioma chip ZLBraG180sur01 were incubated with ARPC5 antibody (16717-1-AP, proteintech, Wuhan, China) and CD3 antibody (17617-1-AP, proteintech, Wuhan, China).

Techniques: Expressing, Quantitative Proteomics

Journal: BMC cancer

Article Title: ARPC5 acts as a potential prognostic biomarker that is associated with cell proliferation, migration and immune infiltrate in gliomas.

doi: 10.1186/s12885-023-11433-w

Figure Lengend Snippet: Fig. 2 Correlation between different status of ARPC5 expression and prognosis of glioma patients. (A) Survival analysis of ARPC5 in CGGA database. (B) ROC curve analysis of ARPC5 at 1, 3, and 5 years in CGGA database. (C) Survival analysis of ARPC5 in TCGA database. (D) ROC curve analysis of ARPC5 at 1, 3, and 5 years in TCGA database. Survival analysis of the signature in patients stratified by grade (E, F), gender (G, H), age (I, J), IDH mutation status (K, L), 1p/19q codeletion status (M, N), and MGMTp methylation status (O, P) in CGGA database

Article Snippet: Glioma tissue samples and glioma chip ZLBraG180sur01 were incubated with ARPC5 antibody (16717-1-AP, proteintech, Wuhan, China) and CD3 antibody (17617-1-AP, proteintech, Wuhan, China).

Techniques: Expressing, Mutagenesis, Methylation

Journal: BMC cancer

Article Title: ARPC5 acts as a potential prognostic biomarker that is associated with cell proliferation, migration and immune infiltrate in gliomas.

doi: 10.1186/s12885-023-11433-w

Figure Lengend Snippet: Fig. 3 Prognostic significance of ARPC5 and its association with drug sensitivity in glioma. (A) Univariate regression analysis of prognosis in CGGA data base. (B) Multivariate analysis of prognosis in CGGA database. (C) The nomogram to predict the association between ARPC5 expression and OS was de veloped using the CGGA dataset. (D) The calibration curve for the nomogram-predicted OS. ARPC5 affects the sensitivity to 5-fluorouracil (E), bleomycin (F), etoposide (G), crizotinib (H), sorafenib (I), and erlotinib (J)

Article Snippet: Glioma tissue samples and glioma chip ZLBraG180sur01 were incubated with ARPC5 antibody (16717-1-AP, proteintech, Wuhan, China) and CD3 antibody (17617-1-AP, proteintech, Wuhan, China).

Techniques: Expressing

Journal: BMC cancer

Article Title: ARPC5 acts as a potential prognostic biomarker that is associated with cell proliferation, migration and immune infiltrate in gliomas.

doi: 10.1186/s12885-023-11433-w

Figure Lengend Snippet: Fig. 4 Enrichment analysis identifies ARPC5-associated signaling pathways. (A) Heatmap for DEGs generated by compare the difference of ARPC5 ex pression in glioma from the CGGA dataset. (B) Volcano plots illustrated all DEGs. Barplot showing the GO (C) and KEGG (D) analysis for ARPC5 in glioma. (E) GO functional annotation of ARPC5 in glioma from GSEA. (F) KEGG pathway analysis of ARPC5 in glioma from GSEA.

Article Snippet: Glioma tissue samples and glioma chip ZLBraG180sur01 were incubated with ARPC5 antibody (16717-1-AP, proteintech, Wuhan, China) and CD3 antibody (17617-1-AP, proteintech, Wuhan, China).

Techniques: Protein-Protein interactions, Generated, Functional Assay

Journal: BMC cancer

Article Title: ARPC5 acts as a potential prognostic biomarker that is associated with cell proliferation, migration and immune infiltrate in gliomas.

doi: 10.1186/s12885-023-11433-w

Figure Lengend Snippet: Fig. 5 Investigations the expression of ARPC5 in glioma through single-cell analysis. (A) Through dimension reduction analysis of CGGA single-cell data, TSNE plot depicted that 6,148 cells were classified into 16 clusters. (B) The violin plot showed the expression of ARPC5 in each type of cluster. (C) Sixteen clusters were divided into 5 types of cells: astrocyte, macrophage, monocyte, epithelial and T cell. (D) The scatter plot shows the expression of ARPC5 in each cell

Article Snippet: Glioma tissue samples and glioma chip ZLBraG180sur01 were incubated with ARPC5 antibody (16717-1-AP, proteintech, Wuhan, China) and CD3 antibody (17617-1-AP, proteintech, Wuhan, China).

Techniques: Expressing, Single-cell Analysis

Journal: BMC cancer

Article Title: ARPC5 acts as a potential prognostic biomarker that is associated with cell proliferation, migration and immune infiltrate in gliomas.

doi: 10.1186/s12885-023-11433-w

Figure Lengend Snippet: Fig. 6 Correlations of ARPC5 expression with immunity-related indexes. (A) Violin plot showed the correlation between immune classification and tumor purity. The immune infiltration models of low- and high-ARPC5 were detected through ssGSEA methods in glioma from the TCGA database. (C) Violin plot showed the correlation between ARPC5 with stromal scores, immune scores, and ESTIMATE scores. (D) Lollipop showed the correlation between ARPC5 with infiltrating immune cells. Scatter plot showed the correlation between ARPC5 with TMB (E) and MSI (F). (G-J) Violin plot showed the correla tion between ARPC5 with immunotherapy

Article Snippet: Glioma tissue samples and glioma chip ZLBraG180sur01 were incubated with ARPC5 antibody (16717-1-AP, proteintech, Wuhan, China) and CD3 antibody (17617-1-AP, proteintech, Wuhan, China).

Techniques: Expressing

Journal: BMC cancer

Article Title: ARPC5 acts as a potential prognostic biomarker that is associated with cell proliferation, migration and immune infiltrate in gliomas.

doi: 10.1186/s12885-023-11433-w

Figure Lengend Snippet: Fig. 7 Effect of ARPC5 on the expression of CD3 and prognosis. (A) Representative images revealed the relationships between the ARPC5 expression and T cell marker CD3 in gliomas. (B) Survival analysis of ARPC5 in glioma chip ZL-BraG180sur01.

Article Snippet: Glioma tissue samples and glioma chip ZLBraG180sur01 were incubated with ARPC5 antibody (16717-1-AP, proteintech, Wuhan, China) and CD3 antibody (17617-1-AP, proteintech, Wuhan, China).

Techniques: Expressing, Marker

Journal: BMC cancer

Article Title: ARPC5 acts as a potential prognostic biomarker that is associated with cell proliferation, migration and immune infiltrate in gliomas.

doi: 10.1186/s12885-023-11433-w

Figure Lengend Snippet: Fig. 8 Effect of ARPC5 on the proliferation and migration of glioma cells. (A) The expression level of ARPC5 protein was detected in LN229 and U251 cells after shRNA interference, GAPDH was used to confirm equal protein loading. (B) RT-qPCR verified the expression efficiency of ARPC5 in LN229 and U251 cells after shRNA interference. Growth curve assessing the effect of ARPC5 on the proliferation of LN229 (C) and U251 (D) cells. (E) The representa tive image showed the clone formation ability of LN229 and U251 cells after transfection. (F) Transwell analysis was further used to examine the effect of ARPC5 on migration of LN229 and U251 cells, and the number of migrating cells was quantitatively analyzed. *p < 0.05, **p < 0.01, ***p < 0.001

Article Snippet: Glioma tissue samples and glioma chip ZLBraG180sur01 were incubated with ARPC5 antibody (16717-1-AP, proteintech, Wuhan, China) and CD3 antibody (17617-1-AP, proteintech, Wuhan, China).

Techniques: Migration, Expressing, shRNA, Quantitative RT-PCR, Transfection

Journal: British Journal of Pharmacology

Article Title: Alcohol enhances the psychostimulant and conditioning effects of mephedrone in adolescent mice; postulation of unique roles of D 3 receptors and BDNF in place preference acquisition

doi: 10.1111/bph.13266

Figure Lengend Snippet: Differential gene expression after microarray analysis

Article Snippet: The cDNA product was used for subsequent real‐time PCR amplification using an ABI Prism 7000 Sequence Detection System (Applied Biosystems) with 25 ng of the cDNA mixture and the assays‐on‐demand from Applied Biosystems Mm00432887_m1 for Drd3 , Mm01350708_m1 for Muted , Mm00444512_m1 for Syt10 , Mm00777068_m1 for Nfu1 , Mm04208715_m1 for Arpc5 , Mm00513979_m1 for Gpx6 , Mm04230607_s1 for BDNF and Mm00607939_s1 for Actb as an endogenous control.

Techniques: Gene Expression, Microarray, Transmission Assay, Binding Assay, Transduction, Sequencing

Journal: International Journal of Legal Medicine

Article Title: Detection of hypoxia markers in the cerebellum after a traumatic frontal cortex injury: a human postmortem gene expression analysis

doi: 10.1007/s00414-014-1129-3

Figure Lengend Snippet: Expression of 14 differentially expressed genes (ARPC5, BCAT, CASP3, FOSB, GFAP, GADD45B, GRIA3, HSD11B1, HSPA12B, IL6, NTRK2, PRPH, RGS6, and S100B) in the cerebellum following a frontal cortex injury. bar refer to the mean values and error bar indicate the standard deviation of gene expression. Data are represented as fold change in gene expression relative to the controls

Article Snippet: We employed TaqMan chemistry (Hs-code in parenthesis) for the detection of our ten mRNA gene candidates which are ARPC5 (hs00271722_m1), BCAT1 (Hs00398962_m1), FOSB (Hs00171851_m1), GADD45B (Hs00169587_m1), GRIA3 (Hs01557466_1), HSD11B1 (Hs01547870_m1), HSPA12B (Hs00369554_m1), IL6 (Hs00985639_m1), PRPH (Hs00196608_m1), and RGS6 (Hs00188763_m1).

Techniques: Expressing, Standard Deviation, Gene Expression

Journal: Nature Communications

Article Title: Inherited ARPC5 mutations cause an actinopathy impairing cell motility and disrupting cytokine signaling

doi: 10.1038/s41467-023-39272-0

Figure Lengend Snippet: a Family pedigrees from patients with ARPC5 variants. Arrows point to the patients studied (P1 in Family 1, P2 in Family 2); Roman numerals indicate generations. b Table depicting the main clinical and laboratory findings in P1 and P2. Negative symbols (−) denote absence; crosses, from (+) to (+++) indicate less to more severe phenotypes, respectively. c Patient 1 images. In the left image, a standing radiograph of P1 shows biconvex thoracolumbar scoliosis with convex right thoracic spinal curvature and convex left thoracolumbar spinal curvature; a right upper lobe pneumatocele is also identified. In the right images (upper, middle and lower, respectively), lung ground glass opacities, multiple abdominal scars product of abnormal wound healing, and right sided myositis of the thigh, are also detected.

Article Snippet: P1’s fibroblasts were transfected with pCMV3-ARPC5-C-HA plasmid (Sino Biological, Cat. HG16494-CY) or mock-transfected (transient; transfection efficiency 20–40%) with empty vector using Amaxa Human Dermal Fibroblast Nucleofector Kit (Lonza, Cat. VPD-1001), program U-023, following manufacturer’s protocol.

Techniques:

Journal: Nature Communications

Article Title: Inherited ARPC5 mutations cause an actinopathy impairing cell motility and disrupting cytokine signaling

doi: 10.1038/s41467-023-39272-0

Figure Lengend Snippet: a Western blotting analysis of patient 1 (P1), her parents, and healthy controls (HC) T-cell blasts lysates with antibodies specific to Arp2/3 complex subunits, as indicated. Vinculin and β-tubulin were used as loading controls. b Western blotting analysis of cell extracts from P1’s and HC’s fibroblasts after native gel electrophoresis. ARPC5- or ARPC2-specific antibodies were used to probe the Arp2/3 complex; GAPDH was used as a loading control. Blots in this figure are representative of at least two independent experiments. The numbers below the western blotting images ( a and b ) represent protein expression levels, quantitatively measured in relation to healthy controls, after normalization to the loading control. Healthy controls’ average value was set at 10. PAGE polyacrylamide gel electrophoresis. Source data are provided as a Source Data file.

Article Snippet: P1’s fibroblasts were transfected with pCMV3-ARPC5-C-HA plasmid (Sino Biological, Cat. HG16494-CY) or mock-transfected (transient; transfection efficiency 20–40%) with empty vector using Amaxa Human Dermal Fibroblast Nucleofector Kit (Lonza, Cat. VPD-1001), program U-023, following manufacturer’s protocol.

Techniques: Western Blot, Nucleic Acid Electrophoresis, Expressing, Polyacrylamide Gel Electrophoresis

Journal: Nature Communications

Article Title: Inherited ARPC5 mutations cause an actinopathy impairing cell motility and disrupting cytokine signaling

doi: 10.1038/s41467-023-39272-0

Figure Lengend Snippet: a Protein expression by immunoblotting of individual Arp2/3 complex subunits in lysates from P1’s fibroblasts transiently transfected (efficiency 20–40%) with empty vector or a plasmid encoding wild-type (WT) ARPC5 . Vinculin was used as a loading control. b Western blotting analysis after native gel electrophoresis of cell extracts from P1’s and healthy control’s (HC) fibroblasts expressing WT ARPC5 by lentiviral transduction. ARPC5- or ARPC2-specific antibodies were used to probe the Arp2/3 complex. GAPDH was used as a loading control. c Immunofluorescence images of representative fibroblasts from P1 transfected with WT ARPC5 (left column) versus cells transfected with empty vector (right column). Cells were seeded on a retronectin-coated surface and fixed after 120 min. d Real-time, impedance-based monitoring of P1’s ARPC5 -rescued fibroblasts versus ARPC5 mock-transduced cells. Impedance values are reported as cell index (CI). Curves represent the mean (±standard deviation) cell index value from four technical replicates. e Wound healing assay with WT ARPC5 -rescued fibroblasts from P1 (middle column) and mock-transduced P1 cells (right column), HC cells were used as controls (left column). The gap length shown at 0 h corresponds to 0.94 mm. All results in this figure are representative of at least two independent experiments. The numbers below the western blotting images represent protein expression levels relative to WT ARPC5 -rescued P1 fibroblasts ( a ) or HC fibroblasts ( b ), after normalization to the loading control. PAGE polyacrylamide gel electrophoresis. Source data are provided as a Source Data file.

Article Snippet: P1’s fibroblasts were transfected with pCMV3-ARPC5-C-HA plasmid (Sino Biological, Cat. HG16494-CY) or mock-transfected (transient; transfection efficiency 20–40%) with empty vector using Amaxa Human Dermal Fibroblast Nucleofector Kit (Lonza, Cat. VPD-1001), program U-023, following manufacturer’s protocol.

Techniques: Expressing, Western Blot, Transfection, Plasmid Preparation, Nucleic Acid Electrophoresis, Transduction, Immunofluorescence, Standard Deviation, Wound Healing Assay, Polyacrylamide Gel Electrophoresis

Journal: Frontiers in Immunology

Article Title: A comprehensively prognostic and immunological analysis of actin-related protein 2/3 complex subunit 5 in pan-cancer and identification in hepatocellular carcinoma

doi: 10.3389/fimmu.2022.944898

Figure Lengend Snippet: The expression levels of APRC5 in different cancers, normal tissues, and cells. (A) The differential expression of ARPC5 in pan-cancer tissues from TCGA datasets. (B) The differential expression of ARPC5 in pan-cancer tissues based on TCGA and GTEx datasets. (C) ARPC5 expression in paired cancer tissues and adjacent normal tissues from TCGA datasets. (D) The mRNA expression levels of ARPC5 in different normal tissues from HPA database. (E) The mRNA expression of ARPC5 in cancer cell lines from HPA database. (F) ARPC5 mRNA expression in different single cell types from HPA database. ns: no significance; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Article Snippet: Afterward, mRNA expression of ARPC5 in different tissues, cell lines, and single-cell types were directly obtained from the Human Protein Atlas website ( https://www.proteinatlas.org/ ).

Techniques: Expressing, Quantitative Proteomics

Journal: Frontiers in Immunology

Article Title: A comprehensively prognostic and immunological analysis of actin-related protein 2/3 complex subunit 5 in pan-cancer and identification in hepatocellular carcinoma

doi: 10.3389/fimmu.2022.944898

Figure Lengend Snippet: Genetic alteration of ARPC5 in pan-cancer. (A) Mutation type and mutation frequency of ARPC5 obtained from the cBioPortal website. (B) The expression levels of ARPC5 in various CNV status of pan-cancer, CNV, copy number variations; *p < 0 . 0 5 ; * *p < 0.01; ****p < 0.0001.

Article Snippet: Afterward, mRNA expression of ARPC5 in different tissues, cell lines, and single-cell types were directly obtained from the Human Protein Atlas website ( https://www.proteinatlas.org/ ).

Techniques: Mutagenesis, Expressing

Journal: Frontiers in Immunology

Article Title: A comprehensively prognostic and immunological analysis of actin-related protein 2/3 complex subunit 5 in pan-cancer and identification in hepatocellular carcinoma

doi: 10.3389/fimmu.2022.944898

Figure Lengend Snippet: Prognosis analyses of ARPC5 in pan-cancer based on univariate Cox regression method. (A) The correlation between ARPC5 expression and OS. (B) The correlation between ARPC5 expression and PFI. (C) The correlation between ARPC5 expression and DSS. OS: overall survival; PFI: progression-free interval; DSS: disease-specific survival.

Article Snippet: Afterward, mRNA expression of ARPC5 in different tissues, cell lines, and single-cell types were directly obtained from the Human Protein Atlas website ( https://www.proteinatlas.org/ ).

Techniques: Expressing

Journal: Frontiers in Immunology

Article Title: A comprehensively prognostic and immunological analysis of actin-related protein 2/3 complex subunit 5 in pan-cancer and identification in hepatocellular carcinoma

doi: 10.3389/fimmu.2022.944898

Figure Lengend Snippet: ARPC5 expression significantly correlated with OS based on Kaplan–Meier analysis. (A) The correlation in ESCA. (B) The correlation in HNSC. (C) The correlation in KIRC. (D) The correlation in KIRP. (E) The correlation in LIHC. (F) The correlation in LGG. (G) The correlation in OV. (H) The correlation in SKCM. The optimal cutoff of ARPC5 expression were used to divide patients into high- and low-expression groups.

Article Snippet: Afterward, mRNA expression of ARPC5 in different tissues, cell lines, and single-cell types were directly obtained from the Human Protein Atlas website ( https://www.proteinatlas.org/ ).

Techniques: Expressing

Journal: Frontiers in Immunology

Article Title: A comprehensively prognostic and immunological analysis of actin-related protein 2/3 complex subunit 5 in pan-cancer and identification in hepatocellular carcinoma

doi: 10.3389/fimmu.2022.944898

Figure Lengend Snippet: The correlation between ARPC5 expression and clinical stage, histologic grade, and tumor molecular subtypes in various cancers based on Spearman’s correlation analysis (the correlation with p < 0.05 were displayed). (A) The correlation between ARPC5 expression and clinical stage in pan-cancer. (B–C) The expression levels of ARPC5 in different clinical stages of KIRC (B) and KIRP (C) . (D) The correlation between ARPC2 expression and histologic grade in pan-cancer. (E–H) The expression levels of ARPC5 in different histologic grades of KIRC (E) , LGG (F) , LIHC (G) , and UCEC (H) . (I–R) The correlation between ARPC5 expression and molecular subtypes in ACC (I) , BRCA (J) , LGG (K) , HNSC (L) , KIRP (M) , OV (N) , LUSC (O) , PCPG (P) , STAD (Q) , UCEC (R) . rho; rank coefficient of Spearman. Pv; p -value. NS, no significance.

Article Snippet: Afterward, mRNA expression of ARPC5 in different tissues, cell lines, and single-cell types were directly obtained from the Human Protein Atlas website ( https://www.proteinatlas.org/ ).

Techniques: Expressing

Journal: Frontiers in Immunology

Article Title: A comprehensively prognostic and immunological analysis of actin-related protein 2/3 complex subunit 5 in pan-cancer and identification in hepatocellular carcinoma

doi: 10.3389/fimmu.2022.944898

Figure Lengend Snippet: The correlation between ARPC5 expression and immune scores and stromal scores of tumor microenvironments in pan-cancer.

Article Snippet: Afterward, mRNA expression of ARPC5 in different tissues, cell lines, and single-cell types were directly obtained from the Human Protein Atlas website ( https://www.proteinatlas.org/ ).

Techniques: Expressing

Journal: Frontiers in Immunology

Article Title: A comprehensively prognostic and immunological analysis of actin-related protein 2/3 complex subunit 5 in pan-cancer and identification in hepatocellular carcinoma

doi: 10.3389/fimmu.2022.944898

Figure Lengend Snippet: The correlation between ARPC5 and immune infiltration cells in pan-cancer based on TIMER algorithm. (A) Heatmap displayed the correlation between ARPC5 expression and the proportions of B cell, CD4 + T cell, CD8 + T cell, neutrophil, macrophage, and DC cell. (B) The top five cancer types (including KIRC, LGG, PRAD, THCA, and THYM) with most significant correlation between ARPC5 and immune infiltration cells were displayed with scatterplots. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Article Snippet: Afterward, mRNA expression of ARPC5 in different tissues, cell lines, and single-cell types were directly obtained from the Human Protein Atlas website ( https://www.proteinatlas.org/ ).

Techniques: Expressing

Journal: Frontiers in Immunology

Article Title: A comprehensively prognostic and immunological analysis of actin-related protein 2/3 complex subunit 5 in pan-cancer and identification in hepatocellular carcinoma

doi: 10.3389/fimmu.2022.944898

Figure Lengend Snippet: The correlation between ARPC5 expression and immune subtypes in pan-cancer using TISIDB. The cancers with significant correlation were displayed. (A) In BLCA. (B) In BRCA. (C) In CESC. (D) In KICH. (E) In KIRC. (F) In LGG. (G) In LIHC. (H) In LUAD. (I) In PAAD. (J) In OV. (K) PCPG. (L) PRAD. (M) In READ. ( N) In SARC. (O) In SKCM. (P) In STAD. (Q) In TGCT. (R) In THCA. (S) In UCS. (T) In UCEC. Pv; p -value. C1, wound healing; C2, IFN-gamma dominant; C3, inflammatory; C4, lymphocyte depleted; C5, immunologically quiet; C6, TGF-b dominant.

Article Snippet: Afterward, mRNA expression of ARPC5 in different tissues, cell lines, and single-cell types were directly obtained from the Human Protein Atlas website ( https://www.proteinatlas.org/ ).

Techniques: Expressing

Journal: Frontiers in Immunology

Article Title: A comprehensively prognostic and immunological analysis of actin-related protein 2/3 complex subunit 5 in pan-cancer and identification in hepatocellular carcinoma

doi: 10.3389/fimmu.2022.944898

Figure Lengend Snippet: The relationship between ARPC5 and immune-checkmate inhibitors biomarkers in pan-cancer. (A) The heatmap showing the co-expression relationship between ARPC5 and 47 immune checkpoint–related genes. (B) Radar plot showing the relationship between ARPC5 and tumor mutation burden (TMB). (C) Radar plot showing the correlation of ARPC5 with microsatellite instability (MSI). (D) Radar plot showing the correlation of ARPC5 with neoantigens. The number in radar plot represents Spearman’s correlation coefficient. * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: Afterward, mRNA expression of ARPC5 in different tissues, cell lines, and single-cell types were directly obtained from the Human Protein Atlas website ( https://www.proteinatlas.org/ ).

Techniques: Expressing, Mutagenesis, Immunopeptidomics

Journal: Frontiers in Immunology

Article Title: A comprehensively prognostic and immunological analysis of actin-related protein 2/3 complex subunit 5 in pan-cancer and identification in hepatocellular carcinoma

doi: 10.3389/fimmu.2022.944898

Figure Lengend Snippet: Correlation analysis between ARPC5 expression and RNA modification-related genes, DNA methyltransferases, and tumor stemness score in 33 cancer types. (A) Co-expression of ARPC5 with m1A-related genes. (B) Co-expression of ARPC5 with m5C-related genes. (C) Co-expression of ARPC5 with m6A-related genes. (D) Co-expression of ARPC5 with DNA methyltransferases. (E) The correlation between ARPC5 expression and Tumor Stemness score (DNAss). (F) The correlation between ARPC5 expression and Tumor Stemness score (RNAss). * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: Afterward, mRNA expression of ARPC5 in different tissues, cell lines, and single-cell types were directly obtained from the Human Protein Atlas website ( https://www.proteinatlas.org/ ).

Techniques: Expressing, RNA modification

Journal: Frontiers in Immunology

Article Title: A comprehensively prognostic and immunological analysis of actin-related protein 2/3 complex subunit 5 in pan-cancer and identification in hepatocellular carcinoma

doi: 10.3389/fimmu.2022.944898

Figure Lengend Snippet: ARPC5 is upregulated in HCC cells and primary HCC tissues. (A) qPCR analysis of ARPC5 mRNA expression in four HCC cell lines (MHCC97-H, Huh7, HCC-LM3, and HepG2) and normal liver cell line (LO2). GAPDH was used as an internal control error bars represent M ± SEM (triplicate experiments). (B, C) The protein expression of ARPC5 was detected in four HCC cell lines and normal liver cell line with Western blot analysis. Error bars represent M ± SD of triplicate measurements. (D) The mRNA expression of ARPC5 in 40 pairs HCC tissues and adjacent para-carcinoma tissues was evaluated using qPCR. (E) Western blot analysis of ARPC5 protein expression in 10 paired HCC tissues and adjacent normal tissues. The number presented the relative protein expression levels of ARPC5. (F) Representative images of ARPC5 immunohistochemical staining analysis in the HCC tissue and adjacent normal liver tissue, original magnifications: ×40 and ×200. Scale bars, 50 μm. (G) Quantitative analysis of ARPC5 expression in HCC tissues based on mean optical density of immunohistochemical staining. Error bars represent the M ± SD of multiple tissues. (H) Kaplan–Meier curves showed that higher expression of ARPC5 was associated with poor DFS in HCC patients. * p < 0.05; ** p < 0.01; *** p < 0.001. ns, no significance.

Article Snippet: Afterward, mRNA expression of ARPC5 in different tissues, cell lines, and single-cell types were directly obtained from the Human Protein Atlas website ( https://www.proteinatlas.org/ ).

Techniques: Expressing, Control, Western Blot, Immunohistochemical staining, Staining

Journal: Frontiers in Immunology

Article Title: A comprehensively prognostic and immunological analysis of actin-related protein 2/3 complex subunit 5 in pan-cancer and identification in hepatocellular carcinoma

doi: 10.3389/fimmu.2022.944898

Figure Lengend Snippet: Silencing of ARPC5 inhibits cell proliferation and promotes cell apoptosis of HCC. (A) The knockdown efficiency of siRNA–ARPC5 was examined in HCC-LM3 and MHCC97-H cells with qPCR. (B) The knockdown efficiency of siRNA-ARPC5 was examined in HCC-LM3 and MHCC97-H cells with Western blot. The number presented as relative protein expression levels of ARPC5. ( C–D ) EdU assays for HCC-LM3 and MHCC 97-H were performed to evaluate cell proliferation ability after transfecting siRNA-ARPC5#1. Representative images (C) and the number of proliferative cells were calculated (D) ; original magnification, ×200. (E–F) Cellular growth curves were evaluated by CCK-8 assays in HCC-LM3 and MHCC97-H cells. (G – H) Flow cytometry was applied to test the apoptosis of HCC cells transfected with si-ARPC5 #1 in HCC-LM3 and MHCC 97-H cells. All data are presented as the M ± SD of three independent experiments. * p < 0.05; ** p < 0.01; *** p < 0.001. ns, no significance.

Article Snippet: Afterward, mRNA expression of ARPC5 in different tissues, cell lines, and single-cell types were directly obtained from the Human Protein Atlas website ( https://www.proteinatlas.org/ ).

Techniques: Knockdown, Western Blot, Expressing, CCK-8 Assay, Flow Cytometry, Transfection

Journal: Frontiers in Immunology

Article Title: A comprehensively prognostic and immunological analysis of actin-related protein 2/3 complex subunit 5 in pan-cancer and identification in hepatocellular carcinoma

doi: 10.3389/fimmu.2022.944898

Figure Lengend Snippet: Role of ARPC5 inhibition on migration, invasion, and epithelial–mesenchymal transition (EMT) of HCC cells. (A–D) Migration ability was assessed by scratch wound healing assay, representative images (A, B) were shown (original magnification, ×200; scale bars, 50 µm), and wound healing areas were calculated (C, D) . (E , F) Transwell assay was applied to examine the invasion ability, representative images (F) were shown (original magnification, ×200; scale bars, 50 µm), and the histogram showed the number of invasion cells (E) . (G) Western blot showed the changes of EMT proteins in HCC-LM3 and MHCC97-H cells transfected with si-ARPC5#1. *p < 0.05; **p < 0.01; ***p < 0.001.

Article Snippet: Afterward, mRNA expression of ARPC5 in different tissues, cell lines, and single-cell types were directly obtained from the Human Protein Atlas website ( https://www.proteinatlas.org/ ).

Techniques: Inhibition, Migration, Wound Healing Assay, Transwell Assay, Western Blot, Transfection

Journal: Frontiers in Immunology

Article Title: A comprehensively prognostic and immunological analysis of actin-related protein 2/3 complex subunit 5 in pan-cancer and identification in hepatocellular carcinoma

doi: 10.3389/fimmu.2022.944898

Figure Lengend Snippet:

Article Snippet: Afterward, mRNA expression of ARPC5 in different tissues, cell lines, and single-cell types were directly obtained from the Human Protein Atlas website ( https://www.proteinatlas.org/ ).

Techniques: Expressing, Mutagenesis, Real-time Polymerase Chain Reaction, Small Interfering RNA

Journal: Journal of Orthopaedic Surgery and Research

Article Title: CPEB2 enhances cell growth and angiogenesis by upregulating ARPC5 mRNA stability in multiple myeloma

doi: 10.1186/s13018-023-03835-0

Figure Lengend Snippet: Primer sequences used for qRT-PCR

Article Snippet: OPM2 cells were fixed with 4% paraformaldehyde and then hybridized with FISH-CPEB2 probe and FISH-ARPC5 probe (Genepharma, Shanghai, China) overnight.

Techniques:

Journal: Journal of Orthopaedic Surgery and Research

Article Title: CPEB2 enhances cell growth and angiogenesis by upregulating ARPC5 mRNA stability in multiple myeloma

doi: 10.1186/s13018-023-03835-0

Figure Lengend Snippet: CPEB2 regulated ARPC5 expression. A ARPC5 mRNA expression levels in the bone marrow CD138+ plasma cells from 20 MM patients and healthy normal donors was measured by qRT-PCR. B ARPC5 protein expression levels in the bone marrow CD138+ plasma cells from five MM patients (M1–M5) and five healthy normal donors (N1–N5) was measured by western blot. C The correlation between CPEB2 and ARPC5 in MM was analyzed by Pearson method. D Western blot analysis was used to detect ARPC5 protein expression in MM cells and nPCs cells. E FISH assay was used to assess the co-localization of CPEB2 and ARPC5 in OPM2 cells. Scale bar, 50 μM. F , G ARPC5 mRNA and protein expression levels were examined by qRT-PCR and western blot analysis in OPM2 cells transfected with sh-CPEB2 or U266 cells transfected with pc-CPEB2. H Actinomycin D was used to assess the stability of ARPC5 in OPM2 cells transfected with sh-CPEB2 or U266 cells transfected with pc-CPEB2. I CHX treatment was performed to assess the effect of sh-CPEB2 or pc-CPEB2 on the half-life of ARPC5. J RIP assay was used to confirmed the interaction between CPEB2 and ARPC5 in U266 cells. Cell experiment was repeated three times. Data were presented as the mean ± SD and analyzed by Student’s t test (for two groups) or one-way ANOVA followed by Tukey post hoc test (for multiple groups). * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: OPM2 cells were fixed with 4% paraformaldehyde and then hybridized with FISH-CPEB2 probe and FISH-ARPC5 probe (Genepharma, Shanghai, China) overnight.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Transfection

Journal: Journal of Orthopaedic Surgery and Research

Article Title: CPEB2 enhances cell growth and angiogenesis by upregulating ARPC5 mRNA stability in multiple myeloma

doi: 10.1186/s13018-023-03835-0

Figure Lengend Snippet: Effects of CPEB2 and ARPC5 on MM cell growth and angiogenesis. OPM2 cells were co-transfected with sh-CPEB2 and pc-ARPC5, and U266 cells were co-transfected with pc-CPEB2 and sh-ARPC5. A , B ARPC5 mRNA and protein expression levels were detected by qRT-PCR and western blot analysis. C CCK8 assay was used to detect cell viability. D Soft-agar colony formation assay was employed to examine colony number. E Cell apoptosis ratio was tested using flow cytometry. F Tube formation assay was performed to measure angiogenesis ability. Cell experiment was repeated three times. Data were expressed as mean ± SD, and one-way ANOVA followed by Tukey post hoc test was used to compare data between multiple groups. ** P < 0.01, *** P < 0.001

Article Snippet: OPM2 cells were fixed with 4% paraformaldehyde and then hybridized with FISH-CPEB2 probe and FISH-ARPC5 probe (Genepharma, Shanghai, China) overnight.

Techniques: Transfection, Expressing, Quantitative RT-PCR, Western Blot, CCK-8 Assay, Soft Agar Assay, Flow Cytometry, Tube Formation Assay

Journal: Journal of Orthopaedic Surgery and Research

Article Title: CPEB2 enhances cell growth and angiogenesis by upregulating ARPC5 mRNA stability in multiple myeloma

doi: 10.1186/s13018-023-03835-0

Figure Lengend Snippet: Effects of CPEB2 knockdown on MM tumor growth in vivo . OPM2 cells transfected with sh-NC or sh-CPEB2 were injected into mice. Tumor volume ( A ), size ( B ) and weight ( C ) were determined in each group. D IHC assay was used to assess Ki-67 positive cells in the tumor tissues of each group. Scale bar, 50 μM E CPEB2 and ARPC5 protein expression levels were determined by western blot analysis in the tumor tissues of each group. n = 6. Data were expressed as mean ± SD, and Student’s t test was used to compare data between two groups. * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: OPM2 cells were fixed with 4% paraformaldehyde and then hybridized with FISH-CPEB2 probe and FISH-ARPC5 probe (Genepharma, Shanghai, China) overnight.

Techniques: In Vivo, Transfection, Injection, Expressing, Western Blot