Journal: PLoS ONE

Article Title: Comparative Transcriptome Analysis of Fetal Skin Reveals Key Genes Related to Hair Follicle Morphogenesis in Cashmere Goats

doi: 10.1371/journal.pone.0151118

Figure Lengend Snippet: (a) E60: KLK11 was detected in the epidermis. The placode of HF shows little or no immunoreactivity at this stage (arrow). (b) E120: KLK11 was expressed strongly in the root sheaths and slightly in the bulb of HF (arrow). (c) NB: KLK11 was present in the hair matrix cells and root sheaths (arrow). (d) E60: VDR shows intense immunostaining in the epidermis. Very little VDR immunoreactivity was detected in the placode (arrow). (e) E120: VDR immunoreactivity is predominantly detected in the ORS keratinocytes and the bulb of HF (arrow). (f) NB: In fully formed HF, VDR staining was present in the root sheaths and bulb cells, where immunoreactivity was particularly enhanced in the ORS keratinocyte and matrix zone. Bar: 100 μm.

Article Snippet: They were then incubated at 4°C overnight in a humidified chamber with the following primary antibodies: VDR (vitamin D receptor) (rabbit, 1:300; Boster, Wuhan, Hubei, China), KLK11 (kallikrein 11) (rabbit, 1:100; Boster, Wuhan, Hubei, China).

Techniques: Immunostaining, Staining

Journal: Biology Open

Article Title: High plasticity in epithelial morphogenesis during insect dorsal closure

doi: 10.1242/bio.20136072

Figure Lengend Snippet: (A–E) WT, (F–I) Tc-zen1 RNAi . (A–I) Confocal projections of early (A,C,D,G,H), and mid (B,E,F,I) DC illustrate several shared features between WT and Tc-zen1 RNAi DC: the straggling organization of the dorsal-most epidermal cells (A1,A2–A2‴,B,F: starred cells), the more lateral F-actin-enriched but unpolarized cardioblast cell row (A3 [partial projection], E,E′: arrows, curly brackets), and amniotic cell elongation around the serosa or amniotic crease (A1,A2,C,G: arrows). In contrast, amniotic F-actin fiber organization differs (D,I: arrows). For comparison, the subsurface cardioblast cell row is superimposed in A1,A2,B,F (paired thin lines). Dashed boxes in A1,A2′,E show enlargements in A2–A3,A2‴,E′, respectively. Horizontal white lines in A2 demarcate segmental boundaries. In the A2‴ schematic and B,F, indicative cells are colored for the lateral epidermis (cyan), dorsal-most epidermis (grey), and amnion (orange). Greater epidermal cell elongation in F than B reflects a slightly older embryo, not a phenotypic difference. The Tc-zen1 RNAi amniotic crease is labeled with arrowheads (G,H). The curly bracket in I marks the anterior ball structure (see also Fig. 1L ). Images are oriented with anterior up and dorsal/medial right. Staining reagents are as indicated (Arm1 is anti-Tc-Arm1). For additional Tc-zen1 RNAi images, see supplementary material Fig. S2 . Abbreviations as in previous figures. Panels C and D show the same two embryos as in Fig. 2C and Fig. 1C , respectively. Scale bars: 20 µm (A,E), 10 µm (A2–A2″,B,E′,F), 5 µm (A2‴), and 100 µm (shown in H for C,D,G–I).

Article Snippet: Additionally, a custom antibody against the C-terminal 15 amino acids of Tc-Armadillo1 (Tc-Arm1: GenBank Accession XR_043141 ( ; )) was used as an adherens junctions marker (produced by Eurogentec, rabbit, 1:10,000, secondary detection as above).

Techniques: Labeling, Staining

Journal: Biology Open

Article Title: High plasticity in epithelial morphogenesis during insect dorsal closure

doi: 10.1242/bio.20136072

Figure Lengend Snippet: (A) Tribolium (WT and Tc-zen1 RNAi ( z1 )), (B) Drosophila, and (C) Oncopeltus are shown at early-mid DC. Upper schematics represent dorsal views, shown to scale, with relative EE areas compared to Drosophila 's (“EE/Fly”) and to total dorsal area (“EE/Egg”) given below ( n ≧3 per species). The ≧ designation for Oncopeltus reflects the limited availability of the youngest, widest DC stages for measurement. Prominent EE features are shown: Tribolium radial (WT) or longitudinal ( Tc-zen1 RNAi ) F-actin fibers and anterior bulge, Drosophila radial contractility gradient from the leading edge F-actin cable, Oncopeltus bilateral thoracic clusters. Lower schematics, in lateral aspect, indicate the number of tissues participating in DC (adapted from ( Drosophila ) and ( Oncopeltus )). (A1–C1) Apical areas of cells at the epidermal–extraembryonic boundary, shown for approximately three epidermal and two amniotic/amnioserosal cell rows, emphasizing the epidermal cell elongation and regularity of the tissue boundary in Drosophila , in contrast to the amniotic elongation and irregular border in Tribolium and Oncopeltus . Schematized representations are based on: anti-Tc-Arm1 ( Fig. 3A ), alpha-Catenin-GFP , and phalloidin , respectively.

Article Snippet: Additionally, a custom antibody against the C-terminal 15 amino acids of Tc-Armadillo1 (Tc-Arm1: GenBank Accession XR_043141 ( ; )) was used as an adherens junctions marker (produced by Eurogentec, rabbit, 1:10,000, secondary detection as above).

Techniques: