Journal: Molecular Vision

Article Title: Ultraviolet B-induced expression of amphiregulin and growth differentiation factor 15 in human lens epithelial cells

doi:

Figure Lengend Snippet: Primers used for RT–PCR analyses.

Article Snippet: Real-time PCR analyses were performed using TaqMan Gene Expression Assays (Applied Biosystems, Carlsbad, CA: Hs00950668_m1 for AREG , Hs00171132_m1 for GDF15 , and Hs99999905_m1 for glyceraldehyde-3-phosphate dehydrogenase [ GAPDH ]).

Techniques:

Journal: Molecular Vision

Article Title: Ultraviolet B-induced expression of amphiregulin and growth differentiation factor 15 in human lens epithelial cells

doi:

Figure Lengend Snippet: UVB-irradiation induced changes in gene expression whose products located in extracellular space.

Article Snippet: Real-time PCR analyses were performed using TaqMan Gene Expression Assays (Applied Biosystems, Carlsbad, CA: Hs00950668_m1 for AREG , Hs00171132_m1 for GDF15 , and Hs99999905_m1 for glyceraldehyde-3-phosphate dehydrogenase [ GAPDH ]).

Techniques: Gene Expression

Journal: Molecular Vision

Article Title: Ultraviolet B-induced expression of amphiregulin and growth differentiation factor 15 in human lens epithelial cells

doi:

Figure Lengend Snippet: RT–PCR and real-time PCR analysis of AREG and GDF15 expression in UVB-irradiated SRA01/04 cells. SRA01/04 cells were exposed at 0, 30, and 50 mJ/cm 2 UVB and total RNAs were extracted 12 h and 24 h later. Relative mRNA abundance of AREG and GDF15 was examined using RT–PCR ( A ) and real-time PCR ( B ). A : RT–PCR products of AREG , GDF15 , and β-actin ( ACTB ) mRNAs. The RNA amounts and PCR cycle numbers were 100 ng and 30 cycles ( AREG ), 100 ng and 29 cycles ( GDF15 ), and 100 ng and 20 cycles ( ACTB ). Aliquots (10 μl) of each RT–PCR product were electrophoresed on 2% agarose gels containing ethidium bromide. B : Relative mRNA levels of AREG (left). Relative mRNA levels of GDF15 (right). Values were normalized with GAPDH mRNA, and compared to values of controls (sham-irradiated cells). Essentially the same results were obtained with three independent experiments, and representative data are shown. *p<0.001, compared to controls.

Article Snippet: Real-time PCR analyses were performed using TaqMan Gene Expression Assays (Applied Biosystems, Carlsbad, CA: Hs00950668_m1 for AREG , Hs00171132_m1 for GDF15 , and Hs99999905_m1 for glyceraldehyde-3-phosphate dehydrogenase [ GAPDH ]).

Techniques: Reverse Transcription Polymerase Chain Reaction, Real-time Polymerase Chain Reaction, Expressing, Irradiation

Journal: Molecular Vision

Article Title: Ultraviolet B-induced expression of amphiregulin and growth differentiation factor 15 in human lens epithelial cells

doi:

Figure Lengend Snippet: AREG and GDF 15 protein level in conditioned media of UVB-exposed cells. SRA01/04 cells were irradiated at indicated energies of UVB. The conditioned media were collected after 12 h and 24 h, and were examined for AREG and GDF15 ELISA assays. Values are expressed as the mean±average deviation in biologic duplicate determinations. Solid triangle and square were AREG protein level at 12 h and 24 h, respectively. Open triangle and square were GDF15 protein level at 12 h and 24 h, respectively. Essentially the same results were obtained with three independent experiments, and representative data are shown. **p<0.01; *p<0.05, compared to control conditioned media (sham-irradiated culture).

Article Snippet: Real-time PCR analyses were performed using TaqMan Gene Expression Assays (Applied Biosystems, Carlsbad, CA: Hs00950668_m1 for AREG , Hs00171132_m1 for GDF15 , and Hs99999905_m1 for glyceraldehyde-3-phosphate dehydrogenase [ GAPDH ]).

Techniques: Irradiation, Enzyme-linked Immunosorbent Assay, Control

Journal: Molecular Vision

Article Title: Ultraviolet B-induced expression of amphiregulin and growth differentiation factor 15 in human lens epithelial cells

doi:

Figure Lengend Snippet: Morphology of primary cultured HLE cells and expression of AREG and GDF15 in UVB-exposed primary cultured HLE cells. The capsular flaps were obtained from 5 patients (A–E) and split in half, and halves were cultured for 2 weeks. A : A typical phase contrast micrograph of primary culltured HLE cells after a 2-week culture is shown. Note that almost all the cells outgrown from the capsules had small, polygonal shapes. One of doublet cultures was irradiated at 50 mJ/cm 2 and another culture was subjected to sham operation. Total RNAs were extracted at 24 h after UVB exposure and relative mRNA levels of AREG and GDF15 were analyzed using real-time PCR ( B ) and RT–PCR ( C ). B : Relative mRNA levels of AREG (upper). Relative mRNA levels of GDF15 (lower). Values were normalized with GAPDH mRNA. The respective values of UVB-exposed culture are compared to values of the corresponding sham-operated cultures (No UVB). *p<0.05, **p<0.01, compared to controls (No UVB). C : RT–PCR products of RNAs from cultures for patient A and B. Aliquots (10 μl) of each RT–PCR product for AREG (20 ng, 38 cycles), GDF15 (20 ng, 33 cycles) and β-actin ( ACTB ; 20 ng, 24 cycles) were electrophoresed on 2% agarose gels containing ethidium bromide.

Article Snippet: Real-time PCR analyses were performed using TaqMan Gene Expression Assays (Applied Biosystems, Carlsbad, CA: Hs00950668_m1 for AREG , Hs00171132_m1 for GDF15 , and Hs99999905_m1 for glyceraldehyde-3-phosphate dehydrogenase [ GAPDH ]).

Techniques: Cell Culture, Expressing, Capsules, Irradiation, Real-time Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction

Journal: Molecular Vision

Article Title: Ultraviolet B-induced expression of amphiregulin and growth differentiation factor 15 in human lens epithelial cells

doi:

Figure Lengend Snippet: Effects of recombinant AREG and GDF15 proteins on cell proliferation and protein synthesis of SRA01/04 cells. Serum starved SRA01/04 cells were incubated with recombinant AREG, GDF15, or EGF at the indicated concentrations and examined 3 H-thymidine ( A ) or 3 H-leucine ( B ) uptake as described in Methods. Values are expressed as the mean±SD (n=4~5) and presented as % of control (none). Essentially the same results were obtained by three times and representative data are shown. ***p<0.001; **p<0.01; *p<0.05, compared to controls (none).

Article Snippet: Real-time PCR analyses were performed using TaqMan Gene Expression Assays (Applied Biosystems, Carlsbad, CA: Hs00950668_m1 for AREG , Hs00171132_m1 for GDF15 , and Hs99999905_m1 for glyceraldehyde-3-phosphate dehydrogenase [ GAPDH ]).

Techniques: Recombinant, Incubation, Control

Journal: Immunity

Article Title: Notch4 signaling limits regulatory T-cell-mediated tissue repair and promotes severe lung inflammation in viral infections

doi: 10.1016/j.immuni.2021.04.002

Figure Lengend Snippet:

Article Snippet: AREG antibody , BiorByt , orb676838.

Techniques: Recombinant, Adjuvant, Enzyme-linked Immunosorbent Assay, Control, Blocking Assay, Mutagenesis, Software