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Image Search Results
Journal: bioRxiv
Article Title: Cis -regulatory evolution in prokaryotes revealed by interspecific archaeal hybrids
doi: 10.1101/044362
Figure Lengend Snippet: The H. volcanii chromosomes of both hybrid cultures as well as the H. mediterranei chromosome of replicate 1 revealed segmental amplifications spanning one or multiple genes (indicated in grey; see supplementary methods). Furthermore, the ratio of copy number among plasmids and main chromosomes varied between the two hybrids. Note that two-fold copy number variation is expected along chromosomes and plasmids as cultures were collected during active DNA replication during log-phase growth . med, mediterranei; vol, volcanii.
Article Snippet: We obtained the genome assemblies and annotations for H. volcanii (strain DS2) and H.
Techniques:
Journal: bioRxiv
Article Title: Cis -regulatory evolution in prokaryotes revealed by interspecific archaeal hybrids
doi: 10.1101/044362
Figure Lengend Snippet: Shown are the (A) ASE values comparing each species’ alleles across replicates; (B) ASE values comparing orthologs in each of the two biological replicates; and (C) as well as the inter-replicate correlation in ASE log-ratios. med, mediterranei; vol, volcanii.
Article Snippet: We obtained the genome assemblies and annotations for H. volcanii (strain DS2) and H.
Techniques:
Journal: bioRxiv
Article Title: Cis -regulatory evolution in prokaryotes revealed by interspecific archaeal hybrids
doi: 10.1101/044362
Figure Lengend Snippet: (A) Approximately equal numbers of orthologs show significant allelic bias favoring either the H. mediterranei (453, red) or H. volcanii allele (476, blue). RPKMs plotted in this figure are the mean of the two biological replicates after normalization (see Methods). med, mediterranei; vol, volcanii. (B) Pairwise comparisons of adjacent genes within predicted operons show significantly more similar ASE than independently transcribed adjacent genes (Kruskal-Wallis rank sum test, p < 10 -15 in both cases). Op, adjacent genes within predicted operons; Non-Op, adjacent genes outside of predicted operons.
Article Snippet: We obtained the genome assemblies and annotations for H. volcanii (strain DS2) and H.
Techniques:
Journal: bioRxiv
Article Title: Cis -regulatory evolution in prokaryotes revealed by interspecific archaeal hybrids
doi: 10.1101/044362
Figure Lengend Snippet: (A) For a set of 19 genes, the expected number with ASE with higher expression from the H. mediterranei alleles is plotted. The kinase gene set had 15/19 genes favoring the H. mediterranei alleles (red arrow), in both biological replicates. (B) H. mediterranei grows robustly in 0.1 mM phosphate, whereas H. volcanii does not. See also Supplemental .
Article Snippet: We obtained the genome assemblies and annotations for H. volcanii (strain DS2) and H.
Techniques: Expressing