Journal: Cancers

Article Title: ARAF Amplification in Small-Cell Lung Cancer-Transformed Tumors Following Resistance to Epidermal Growth Factor Receptor–Tyrosine Kinase Inhibitors

doi: 10.3390/cancers16203501

Figure Lengend Snippet: The distribution of ARAF copy numbers and clinicopathologic characteristics is shown for patients resistant to first– and second–generation EGFR-TKIs ( A , B ) and Osimertinib ( C , D ). B and D provide the percentages of each characteristic within their respective categories. Blue represents ARAF non-amplified cases, while orange indicates ARAF amplified cases.

Article Snippet: The DNA from re-biopsied samples were screened for ARAF amplification using a TaqMan gene copy number assay (assay ID: Hs05691600_cn, Applied Biosystems, Waltham, MA, USA).

Techniques: Amplification

Journal: Cancers

Article Title: ARAF Amplification in Small-Cell Lung Cancer-Transformed Tumors Following Resistance to Epidermal Growth Factor Receptor–Tyrosine Kinase Inhibitors

doi: 10.3390/cancers16203501

Figure Lengend Snippet: Cases with ARAF amplification in SCLC-transformed tumors following resistance to EGFR-TKI. ( A ) Case with SCLC transformation after resistance to erlotinib treatment. A pre-treatment sample was taken at diagnosis using transbronchial lung biopsy (TBLB), and re-biopsied samples were taken at resistance to erlotinib treatment using CT-Guided needle biopsy (CTNB). Scale bar, 100 µm for low-power field and 50 µm for high-power field in CD56. IRI, irinotecan; AMR, amrubicin; DTX, docetaxel; PEM, pemetrexed. ( B ) Case with SCLC transformation after resistance to Osimertinib treatment. A pre-treatment sample was taken after resistance to gefitinib using CTNB, and re-biopsied samples were taken at resistance to Osimertinib using ultrasound-guided needle biopsy (USNB). Scale bar, 100 µm. ETP, etoposide. ( C ) Log 2-fold change in expression of the indicated genes at the time of resistance compared to the pre-treatment biopsy samples shown in ( A , B ). ( D ) GSEA data for the indicated signatures. ( E ) Immunohistochemistry staining for indicated antibodies. Scale bar, 100 µm for low-power field and 50 µm for high-power field in YAP.

Article Snippet: The DNA from re-biopsied samples were screened for ARAF amplification using a TaqMan gene copy number assay (assay ID: Hs05691600_cn, Applied Biosystems, Waltham, MA, USA).

Techniques: Amplification, Transformation Assay, Biomarker Discovery, Expressing, Immunohistochemistry, Staining