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Cycles of antidote/retriever regulation in <t>aPTT</t> assays using ( A ) Apt P; ( B ) 2HT-BnPn and ( C ) 2HT-BPPB. Addition of antidote reverses anticoagulation to less than 10%, whereas addition of retriever I restores it to almost 80%. Retriever II reverses anticoagulation back to less than 10%. The % anticoagulant activity is calculated as described in Materials and Methods. Error bars represent SEM. Raw clotting times are provided in the .
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Cycles of antidote/retriever regulation in <t>aPTT</t> assays using ( A ) Apt P; ( B ) 2HT-BnPn and ( C ) 2HT-BPPB. Addition of antidote reverses anticoagulation to less than 10%, whereas addition of retriever I restores it to almost 80%. Retriever II reverses anticoagulation back to less than 10%. The % anticoagulant activity is calculated as described in Materials and Methods. Error bars represent SEM. Raw clotting times are provided in the .
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Cycles of antidote/retriever regulation in <t>aPTT</t> assays using ( A ) Apt P; ( B ) 2HT-BnPn and ( C ) 2HT-BPPB. Addition of antidote reverses anticoagulation to less than 10%, whereas addition of retriever I restores it to almost 80%. Retriever II reverses anticoagulation back to less than 10%. The % anticoagulant activity is calculated as described in Materials and Methods. Error bars represent SEM. Raw clotting times are provided in the .
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Trinity Biotech triniclot aptt hs reagent
Cycles of antidote/retriever regulation in <t>aPTT</t> assays using ( A ) Apt P; ( B ) 2HT-BnPn and ( C ) 2HT-BPPB. Addition of antidote reverses anticoagulation to less than 10%, whereas addition of retriever I restores it to almost 80%. Retriever II reverses anticoagulation back to less than 10%. The % anticoagulant activity is calculated as described in Materials and Methods. Error bars represent SEM. Raw clotting times are provided in the .
Triniclot Aptt Hs Reagent, supplied by Trinity Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cycles of antidote/retriever regulation in <t>aPTT</t> assays using ( A ) Apt P; ( B ) 2HT-BnPn and ( C ) 2HT-BPPB. Addition of antidote reverses anticoagulation to less than 10%, whereas addition of retriever I restores it to almost 80%. Retriever II reverses anticoagulation back to less than 10%. The % anticoagulant activity is calculated as described in Materials and Methods. Error bars represent SEM. Raw clotting times are provided in the .
Triniclot Aptt Hs Fosfolipídeos Purificados (Porco E Galinha), supplied by Trinity Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cycles of antidote/retriever regulation in <t>aPTT</t> assays using ( A ) Apt P; ( B ) 2HT-BnPn and ( C ) 2HT-BPPB. Addition of antidote reverses anticoagulation to less than 10%, whereas addition of retriever I restores it to almost 80%. Retriever II reverses anticoagulation back to less than 10%. The % anticoagulant activity is calculated as described in Materials and Methods. Error bars represent SEM. Raw clotting times are provided in the .
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Cycles of antidote/retriever regulation in <t>aPTT</t> assays using ( A ) Apt P; ( B ) 2HT-BnPn and ( C ) 2HT-BPPB. Addition of antidote reverses anticoagulation to less than 10%, whereas addition of retriever I restores it to almost 80%. Retriever II reverses anticoagulation back to less than 10%. The % anticoagulant activity is calculated as described in Materials and Methods. Error bars represent SEM. Raw clotting times are provided in the .
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Assays Triniclot Aptt S Tcoag Ireland Limited Ca, supplied by Tcoag Ireland Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Cycles of antidote/retriever regulation in aPTT assays using ( A ) Apt P; ( B ) 2HT-BnPn and ( C ) 2HT-BPPB. Addition of antidote reverses anticoagulation to less than 10%, whereas addition of retriever I restores it to almost 80%. Retriever II reverses anticoagulation back to less than 10%. The % anticoagulant activity is calculated as described in Materials and Methods. Error bars represent SEM. Raw clotting times are provided in the .

Journal: Molecules

Article Title: Design of Potent and Controllable Anticoagulants Using DNA Aptamers and Nanostructures

doi: 10.3390/molecules21020202

Figure Lengend Snippet: Cycles of antidote/retriever regulation in aPTT assays using ( A ) Apt P; ( B ) 2HT-BnPn and ( C ) 2HT-BPPB. Addition of antidote reverses anticoagulation to less than 10%, whereas addition of retriever I restores it to almost 80%. Retriever II reverses anticoagulation back to less than 10%. The % anticoagulant activity is calculated as described in Materials and Methods. Error bars represent SEM. Raw clotting times are provided in the .

Article Snippet: CaCl 2 solution and TriniClot aPTT reagent were purchased from Trinity Biotech USA (Jamestown, NY, USA).

Techniques: Activity Assay, Coagulation

KEY RESOURCE TABLE

Journal: Cell chemical biology

Article Title: Anti-PEG antibodies inhibit the anticoagulant activity of PEGylated aptamers

doi: 10.1016/j.chembiol.2019.02.001

Figure Lengend Snippet: KEY RESOURCE TABLE

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken polyclonal anti-human IgG-HRP Abcam Ca# 6864 Rabbit anti-monkey IgG-HRP Sigma-Aldrich Ca# 2054 Mouse anti-PEG Life Diagnostics Ca# ID9-6 Bacterial and Virus Strains Biological Samples Rhesus monkey plasma Tarantal, UC Davis N/A Human plasma samples Duke University; Department of Medicine N/A Pooled normal human plasma George King Biomedical Ca# 0010-1 Chemicals, Peptides, and Recombinant Proteins Human Factor IXa Haematologic Technologies Ca# HCIXA-0050 Adenosine deaminase bovine (ADA) Sigma-Aldrich A5168; CAS: 9026-93-1 ADAGEN (pegademase bovine) Lediant Biosciences http://www.adagen.com/pdf/AdagenPI.pdf Macugen (pegaptanib) Bausch and Lomb http://www.bausch.com/Portals/69/-/m/BL/United%20States/USFiles/Package%20Inserts/Pharma/macugen-package-insert.pdf Critical Commercial Assays TriniCLOT aPTT S Tcoag Ireland Limited Ca# T1201 Deposited Data Experimental Models: Cell Lines Experimental Models: Organisms/Strains Mouse: C57/BL/6J Jackson Laboratories JAX: 000664 Oligonucleotides PEGylated RNA aptamer RB006 sequence: 40 kDa mPEG-GUGGAcuAuAccGcGuAAuGcU(G)CcuCCACidT: (uppercase-2’ F, lowercase-2’O Methyl, ()-unmodified) Dyke et al., 2006 N/A UnPEGylated RNA aptamer RB005 sequence: GUGGAcuAuAccGcGuAAuGcU(G)CcuCCAC-idT: (uppercase-2’ F, lowercase-2’O Methyl, ()-unmodified) Dyke et al., 2006 N/A Recombinant DNA Software and Algorithms Biacore T200 control software GE Healthcare - Life Sciences Ca# 29148695 T200 evaluation software GE Healthcare - Life Sciences Ca# BR-1005-97 LabChart v8.1.10 Mac ADInstruments https://www.adinstruments.com/support/downloads/mac/labchart Other PEG-BSA coated plates Life Diagnostics Ca# PBS-01 Transonic flow probe Transonic Ca# MA0.5PSB Biacore T200 instrument GE Healthcare - Life Sciences Ca# 28975001 CM5 sensor chip GE Healthcare - Life Sciences Ca# BR-100012 Open in a separate window KEY RESOURCE TABLE Anti-PEG antibodies bind to PEGylated RNA aptamer RB006 Anti-PEG antibodies inhibit anticoagulant aptamer activity in vitro and in vivo After one administration of RB006 anti-PEG antibodies were found in rhesus monkeys

Techniques: Virus, Clinical Proteomics, Recombinant, Sequencing, Software, Control