Journal: Frontiers in Physiology

Article Title: β-Cyclodextrins Decrease Cholesterol Release and ABC-Associated Transporter Expression in Smooth Muscle Cells and Aortic Endothelial Cells

doi: 10.3389/fphys.2016.00185

Figure Lengend Snippet: Cholesterol efflux from ABAE and SMCs . Cells were first labeled with [ 3 H]-cholesterol (0.5 μCi/mL) for 36 h at 37°C and then equilibrated for 24 h in DMEM/0.05% BSA at 37°C. Cellular [ 3 H]-cholesterol loads in DPM per μg of proteins were compared between ABAE and SMCs (A) . Data represent the mean ± SEM (in DPM/μg of proteins), with n = 6 from two sets of experiments. Paired- t -test was performed, *** p < 0.001 when compared with ABAE condition. [ 3 H]-cholesterol released from both cell types was then measured 6 h after medium supplementation with either BSA (control condition) or cholesterol acceptors [ApoA-I (20 μg/mL) or HDL (50 μg/mL)] at 37°C (B) . Data represent the mean ± SEM (in DPM/μg of proteins), with n = 6 from two sets of experiments. One-way ANOVA test followed by Bonferroni correction was performed in which *** p < 0.001 refers to the control condition and ### p < 0.001 refers to the matched HDL condition in ABAE.

Article Snippet: ApoA-I and HDL were purchased from PROSCI Incorporated (Poway, CA, USA).

Techniques: Labeling, Control

Journal: Frontiers in Physiology

Article Title: β-Cyclodextrins Decrease Cholesterol Release and ABC-Associated Transporter Expression in Smooth Muscle Cells and Aortic Endothelial Cells

doi: 10.3389/fphys.2016.00185

Figure Lengend Snippet: Cholesterol release from ABAE (A,C) and from SMCs (B,D) upon CD-treatment . Cells were first labeled with [ 3 H]-cholesterol (0.5 μCi/mL) for 36 h at 37°C and then equilibrated in DMEM/0.05% BSA for 24 h at 37°C. [ 3 H]-cholesterol released in the medium from both cell types was then measured 6 h after medium supplementation with cholesterol acceptors [ApoA-I (20 μg/mL) or HDL (50 μg/mL)] at 37°C. The radioactivity contained in the culture medium, that corresponds to the cholesterol released from cells was measured. Data are expressed as the mean ± SEM (in DPM/μg of proteins, n = 6 from two sets of experiments). Statistical analysis: a one-way ANOVA followed by Dunnett's test for multiple comparisons in which * p < 0.05; *** p < 0.001 compared with the control condition (open bars) without any CD treatment.

Article Snippet: ApoA-I and HDL were purchased from PROSCI Incorporated (Poway, CA, USA).

Techniques: Labeling, Radioactivity, Control

Journal: Frontiers in Physiology

Article Title: β-Cyclodextrins Decrease Cholesterol Release and ABC-Associated Transporter Expression in Smooth Muscle Cells and Aortic Endothelial Cells

doi: 10.3389/fphys.2016.00185

Figure Lengend Snippet: Effect of 10 μM T0901317 on cholesterol efflux to ApoA-I and HDL in ABAE (A) and SMCs (B) treated or nor with Rameβ . Cells were first labeled with [ 3 H]-cholesterol as previously described and incubated in the presence of 1 mM Rameβ, 10 μM T0901317 or both during 24 h. Then, [ 3 H]-cholesterol released in the medium from both cell types was measured 6 h after medium supplementation with either ApoA-I (20 μg/mL) or HDL (50 μg/mL). Data are expressed as the mean ± SEM (in DPM/μg of proteins, n = 6 from two sets of experiments). Control condition (without any treatment) is used as the reference level and set to 100%. Statistical analysis: a one-way ANOVA followed by Dunnett's test for multiple comparisons in which * p < 0.05; ** p < 0.01; *** p < 0.001 compared with the untreated condition (Control condition, open bars) and in which ## p < 0.01 when Rameβ + T0901317 condition was compared with Rameβ condition.

Article Snippet: ApoA-I and HDL were purchased from PROSCI Incorporated (Poway, CA, USA).

Techniques: Labeling, Incubation, Control

Journal: PLoS ONE

Article Title: Genome wide profiling in oral squamous cell carcinoma identifies a four genetic marker signature of prognostic significance

doi: 10.1371/journal.pone.0174865

Figure Lengend Snippet: Concordance percentage for amplification of LRP12 (chr 8q), CCND1 (chr 11q), TPM2 (chr 9p), FSCN1 (chr 7p), EGFR (chr 7p), CLPTM1L (chr 5p) and deletion of CHL1 (chr 3p) and CSMD1 (chr 8p) identified using array CGH and validated using qPCR copy number analysis in OSCC samples.

Article Snippet: Copy number analysis was done on 66 OSCCs using TaqMan Copy Number Assay: LRP12 (Hs01987319_cn), FSCN1 (Hs03631914_cn), EGFR (Hs02309320_cn), CCND1 (Hs02226007_cn), CHL1 (Hs02163529_cn), TPM2 (Hs01060645_cn), CLPTM1L (Hs01133209_cn), CSMD1 (Hs03683117_cn) (Applied Biosystems, Foster City, CA, USA).

Techniques: Amplification

Journal: PLoS ONE

Article Title: Genome wide profiling in oral squamous cell carcinoma identifies a four genetic marker signature of prognostic significance

doi: 10.1371/journal.pone.0174865

Figure Lengend Snippet: Overall survival curves were analyzed according to ≥ 1 of genetic marker (EGFR, CCND1, TPM2 and LRP12) using Kaplan-Meier estimate with log-rank test.

Article Snippet: Copy number analysis was done on 66 OSCCs using TaqMan Copy Number Assay: LRP12 (Hs01987319_cn), FSCN1 (Hs03631914_cn), EGFR (Hs02309320_cn), CCND1 (Hs02226007_cn), CHL1 (Hs02163529_cn), TPM2 (Hs01060645_cn), CLPTM1L (Hs01133209_cn), CSMD1 (Hs03683117_cn) (Applied Biosystems, Foster City, CA, USA).

Techniques: Marker

Journal: PLoS ONE

Article Title: Genome wide profiling in oral squamous cell carcinoma identifies a four genetic marker signature of prognostic significance

doi: 10.1371/journal.pone.0174865

Figure Lengend Snippet: Multivariate cox regression model analysis of four combined genetic markers consisting of EGFR, TPM2, CCND1 and LRP12 in OSCC overall survival.

Article Snippet: Copy number analysis was done on 66 OSCCs using TaqMan Copy Number Assay: LRP12 (Hs01987319_cn), FSCN1 (Hs03631914_cn), EGFR (Hs02309320_cn), CCND1 (Hs02226007_cn), CHL1 (Hs02163529_cn), TPM2 (Hs01060645_cn), CLPTM1L (Hs01133209_cn), CSMD1 (Hs03683117_cn) (Applied Biosystems, Foster City, CA, USA).

Techniques: Marker

Journal: Translational Vision Science & Technology

Article Title: SZN-413, a FZD4 Agonist, as a Potential Novel Therapeutic for the Treatment of Diabetic Retinopathy

doi: 10.1167/tvst.11.9.19

Figure Lengend Snippet: FZD4 and LRP5, primary receptors for Wnt signaling in vascular endothelial cells. (A) FZD1–10 and LRP5 and LRP6 mRNA expression in HRMECs, bEnd.3 cells, and HUVECs quantified by quantitative polymerase chain reaction (qPCR) and normalized by the housekeeping gene Actin . Bars represent mean ± SD. (B) Data from Macosko et al. 31 were reanalyzed to derive scaled average gene expression by cell type of Wnt receptors in mouse retina. (C) Data from Menon et al. were reanalyzed to derive scaled average gene expression by cell type of Wnt receptors in human retina.

Article Snippet: RNA was quantified using TaqMan Fast Advanced Master Mix (Thermo Fisher Scientific) with the following Thermo Fisher Scientific probes: Mm00445405_s1 Fzd1 , Mm02524776_s1 Fzd2 , Mm00445423_m1 Fzd3 , Mm00433382_m1 Fzd4 , Mm00445623_s1 Fzd5 , Mm00433387_m1 Fzd6 , Mm00433409_s1 Fzd7 , Mm01234717_s1 Fzd8 , Mm01206511_s1 Fzd9 , Mm00558396_s1 Fzd10 , Mm01227476_m1 Lrp5 , Mm00999795_m1 Lrp6 , Hs00268943_s1 FZD1 , Hs00361432_s1 FZD2 , Hs00907280_m1 FZD3 , Hs00201853_m1 FZD4 , Hs00258278_s1 FZD5 , Hs01095627_m1 FZD6 , Hs00275833_s1 FZD7 , Hs00259040_s1 FZD8 , Hs00268954_s1 FZD9 , Hs00273077_s1 FZD10 , Hs00182031_m1 LRP5 , Hs00233945_m1 LRP6 , Hs01547250_m1 LEF1 , Hs00293017_m1 MFSD2A , Hs00610344-m1 AXIN2 , and Mm00443610_m1 Axin2 .

Techniques: Expressing, Real-time Polymerase Chain Reaction, Gene Expression