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Image Search Results
Journal: Marine Drugs
Article Title: Identification of a New Antimicrobial, Desertomycin H, Utilizing a Modified Crowded Plate Technique
doi: 10.3390/md19080424
Figure Lengend Snippet: Traditional crowded plate technique (CPT) and modified crowded plate technique (mCPT) on nutrient-poor media. ( A , B ) Representative samples of Waksman’s CPT. Antibiotic-producing bacteria (arrows) can be identified by the zones of inhibition surrounding individual colonies following two weeks of incubation at 30 °C on TYME media. ( B ) Antibiotic-producing bacteria (arrows) surrounded by lysed cells forming a zone of inhibition. ( C ) Modified crowded plate technique using a d -alanine auxotrophic strain of E. coli pre-inoculated onto the entire plate as a target organism. A diluted soil sample is then immediately spread on the plate. Antibiotic-producing bacteria (arrows) can be identified by the zones of inhibition surrounding individual colonies following two weeks of incubation at 30 °C on TYME media.
Article Snippet: PP742 ,
Techniques: Modification, Bacteria, Inhibition, Incubation
Journal: Marine Drugs
Article Title: Identification of a New Antimicrobial, Desertomycin H, Utilizing a Modified Crowded Plate Technique
doi: 10.3390/md19080424
Figure Lengend Snippet: Spread-patch assay confirmation testing of purified antibiotic producers using drug-sensitive E. coli or all 15 combined Antibiotic Resistance Panel (15X-ARP) E. coli strains. ( A ) Isolates PAP100 (left) and PAP117 (right) were tested on drug-sensitive E. coli with both showing clear zones of inhibition. ( B ) Isolates PAP100 (left) and PAP117 (right) were tested on 15X-ARP E. coli strains with PAP100 showing no zone of inhibition and PAP117 showing a large zone of inhibition. For PAP100, the zone of inhibition was also lost when E. coli carrying only the bla NDM-1 was used in the spread-patch assay. EPSM plates were incubated at 30 °C and imaged at 7 days post-inoculation.
Article Snippet: PP742 ,
Techniques: Purification, Inhibition, Incubation
Journal: Marine Drugs
Article Title: Identification of a New Antimicrobial, Desertomycin H, Utilizing a Modified Crowded Plate Technique
doi: 10.3390/md19080424
Figure Lengend Snippet: Bacterial strains used in this study.
Article Snippet: PP742 ,
Techniques: