apg-115 Search Results


apg 115  (MedChemExpress)
93
MedChemExpress apg 115
<t>APG-115</t> <t>restores</t> p53 expression and activity. mRNA expression of MDM2 , TP53 , and CDKN1A/p21 were detected in (A,B) CLL patient primary cells and (C,D) EHEB cells treated with different concentrations APG-115 or different times of APG-115 (10 μM). Relative gene expression was calculated based on the threshold cycle (Ct) values and normalized to β-actin internal control using the 2 −ΔΔCt method. Experiments were performed in triplicate and repeated at least three times. The results showed representative primary cells from three CLL patients. (E) The protein expressions of MDM2, p53, and p21 in EHEB cells were detected by WB. (F) The quantification analysis of protein bands in panel E was performed using ImageJ software; the values of protein bands were divided by the internal control β-actin. The data shown are representative images of three independent experiments.
Apg 115, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/apg 115/product/MedChemExpress
Average 93 stars, based on 1 article reviews
apg 115 - by Bioz Stars, 2026-03
93/100 stars
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apg-115  (Ascentage Pharma)
90
Ascentage Pharma apg-115
<t>APG-115</t> <t>restores</t> p53 expression and activity. mRNA expression of MDM2 , TP53 , and CDKN1A/p21 were detected in (A,B) CLL patient primary cells and (C,D) EHEB cells treated with different concentrations APG-115 or different times of APG-115 (10 μM). Relative gene expression was calculated based on the threshold cycle (Ct) values and normalized to β-actin internal control using the 2 −ΔΔCt method. Experiments were performed in triplicate and repeated at least three times. The results showed representative primary cells from three CLL patients. (E) The protein expressions of MDM2, p53, and p21 in EHEB cells were detected by WB. (F) The quantification analysis of protein bands in panel E was performed using ImageJ software; the values of protein bands were divided by the internal control β-actin. The data shown are representative images of three independent experiments.
Apg 115, supplied by Ascentage Pharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/apg-115/product/Ascentage Pharma
Average 90 stars, based on 1 article reviews
apg-115 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
apg-115  (Wolters Kluwer Health)
90
Wolters Kluwer Health apg-115
<t>APG-115</t> <t>restores</t> p53 expression and activity. mRNA expression of MDM2 , TP53 , and CDKN1A/p21 were detected in (A,B) CLL patient primary cells and (C,D) EHEB cells treated with different concentrations APG-115 or different times of APG-115 (10 μM). Relative gene expression was calculated based on the threshold cycle (Ct) values and normalized to β-actin internal control using the 2 −ΔΔCt method. Experiments were performed in triplicate and repeated at least three times. The results showed representative primary cells from three CLL patients. (E) The protein expressions of MDM2, p53, and p21 in EHEB cells were detected by WB. (F) The quantification analysis of protein bands in panel E was performed using ImageJ software; the values of protein bands were divided by the internal control β-actin. The data shown are representative images of three independent experiments.
Apg 115, supplied by Wolters Kluwer Health, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/apg-115/product/Wolters Kluwer Health
Average 90 stars, based on 1 article reviews
apg-115 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


APG-115 restores p53 expression and activity. mRNA expression of MDM2 , TP53 , and CDKN1A/p21 were detected in (A,B) CLL patient primary cells and (C,D) EHEB cells treated with different concentrations APG-115 or different times of APG-115 (10 μM). Relative gene expression was calculated based on the threshold cycle (Ct) values and normalized to β-actin internal control using the 2 −ΔΔCt method. Experiments were performed in triplicate and repeated at least three times. The results showed representative primary cells from three CLL patients. (E) The protein expressions of MDM2, p53, and p21 in EHEB cells were detected by WB. (F) The quantification analysis of protein bands in panel E was performed using ImageJ software; the values of protein bands were divided by the internal control β-actin. The data shown are representative images of three independent experiments.

Journal: Frontiers in Pharmacology

Article Title: MDM2 inhibitor APG-115 synergizes with ABT-199 to induce cell apoptosis in chronic lymphocytic leukemia

doi: 10.3389/fphar.2024.1441383

Figure Lengend Snippet: APG-115 restores p53 expression and activity. mRNA expression of MDM2 , TP53 , and CDKN1A/p21 were detected in (A,B) CLL patient primary cells and (C,D) EHEB cells treated with different concentrations APG-115 or different times of APG-115 (10 μM). Relative gene expression was calculated based on the threshold cycle (Ct) values and normalized to β-actin internal control using the 2 −ΔΔCt method. Experiments were performed in triplicate and repeated at least three times. The results showed representative primary cells from three CLL patients. (E) The protein expressions of MDM2, p53, and p21 in EHEB cells were detected by WB. (F) The quantification analysis of protein bands in panel E was performed using ImageJ software; the values of protein bands were divided by the internal control β-actin. The data shown are representative images of three independent experiments.

Article Snippet: APG-115 was obtained from Suzhou Ascentage Pharma Co., Ltd. ABT-199 was a product of MCE.

Techniques: Expressing, Activity Assay, Gene Expression, Control, Software

APG-115 inhibits cellular viability and induces apoptosis. (A) EHEB cells were treated with different concentrations of APG-115 for 48 h, cell viability was assessed by MTS assay; (B) cell apoptosis was detected by flow cytometry. Experiments were performed in triplicate and repeated at least three times. (C) Cell apoptosis was detected on CLL patient primary cells (P) (n = 8) cultured with 10 ng CD40L and IL-4 and treated with APG-115 for 48 h. Data was expressed as mean ± standard deviation. Statistical significance was determined using the Student unpaired t -test, with Welch’s correction. *, compared to the control group (no APG-115 treatment) * p < 0.05, ** p < 0.01, *** p < 0.001. Non-significant results are denoted by ns. (D) Protein expression of caspase-3 and PARP was detected by WB in EHEB cells after 12-h treatment with APG-115. β-actin was a control protein. (E) Quantification analysis of the protein bands in D using ImageJ software; the values were divided by the internal control β-actin. The data shown are representative images of two independent experiments. (F) Statistical histogram of cell cycle in EHEB cells exposed to increasing concentrations of APG-115 for 48 h.

Journal: Frontiers in Pharmacology

Article Title: MDM2 inhibitor APG-115 synergizes with ABT-199 to induce cell apoptosis in chronic lymphocytic leukemia

doi: 10.3389/fphar.2024.1441383

Figure Lengend Snippet: APG-115 inhibits cellular viability and induces apoptosis. (A) EHEB cells were treated with different concentrations of APG-115 for 48 h, cell viability was assessed by MTS assay; (B) cell apoptosis was detected by flow cytometry. Experiments were performed in triplicate and repeated at least three times. (C) Cell apoptosis was detected on CLL patient primary cells (P) (n = 8) cultured with 10 ng CD40L and IL-4 and treated with APG-115 for 48 h. Data was expressed as mean ± standard deviation. Statistical significance was determined using the Student unpaired t -test, with Welch’s correction. *, compared to the control group (no APG-115 treatment) * p < 0.05, ** p < 0.01, *** p < 0.001. Non-significant results are denoted by ns. (D) Protein expression of caspase-3 and PARP was detected by WB in EHEB cells after 12-h treatment with APG-115. β-actin was a control protein. (E) Quantification analysis of the protein bands in D using ImageJ software; the values were divided by the internal control β-actin. The data shown are representative images of two independent experiments. (F) Statistical histogram of cell cycle in EHEB cells exposed to increasing concentrations of APG-115 for 48 h.

Article Snippet: APG-115 was obtained from Suzhou Ascentage Pharma Co., Ltd. ABT-199 was a product of MCE.

Techniques: MTS Assay, Flow Cytometry, Cell Culture, Standard Deviation, Control, Expressing, Software

APG-115 inhibits the expression of BCL-2, BCL-xL, and MCL-1. (A) EHEB cells and (B) CLL patient primary cells were treated with different concentrations of APG-115 for 12 h. The protein expression of BCL-2, BCL-xL, MCL-1, and BAX were detected by WB, and β-actin was the internal control protein. (C,D) The quantification analysis of protein bands in (A,B) by ImageJ software, a statistical graph showing the value divided by the internal control and compared with the control group without treatment.

Journal: Frontiers in Pharmacology

Article Title: MDM2 inhibitor APG-115 synergizes with ABT-199 to induce cell apoptosis in chronic lymphocytic leukemia

doi: 10.3389/fphar.2024.1441383

Figure Lengend Snippet: APG-115 inhibits the expression of BCL-2, BCL-xL, and MCL-1. (A) EHEB cells and (B) CLL patient primary cells were treated with different concentrations of APG-115 for 12 h. The protein expression of BCL-2, BCL-xL, MCL-1, and BAX were detected by WB, and β-actin was the internal control protein. (C,D) The quantification analysis of protein bands in (A,B) by ImageJ software, a statistical graph showing the value divided by the internal control and compared with the control group without treatment.

Article Snippet: APG-115 was obtained from Suzhou Ascentage Pharma Co., Ltd. ABT-199 was a product of MCE.

Techniques: Expressing, Control, Software

APG-115 suppresses the activation of AKT and ERK signaling pathways (A) The protein expression of AKT, p-AKT, ERK, and p-ERK were detected by WB in EHEB cells and (B) CLL patient primary cells treated with different concentrations of APG-115 for 12 h. β-actin was the internal reference protein. The results were representatives of primary cells from three CLL patients. (C,D) The quantification analysis of protein bands in (A,B) by ImageJ software, a statistical graph showing the value divided by the internal reference protein β-actin, and compared with the control group.

Journal: Frontiers in Pharmacology

Article Title: MDM2 inhibitor APG-115 synergizes with ABT-199 to induce cell apoptosis in chronic lymphocytic leukemia

doi: 10.3389/fphar.2024.1441383

Figure Lengend Snippet: APG-115 suppresses the activation of AKT and ERK signaling pathways (A) The protein expression of AKT, p-AKT, ERK, and p-ERK were detected by WB in EHEB cells and (B) CLL patient primary cells treated with different concentrations of APG-115 for 12 h. β-actin was the internal reference protein. The results were representatives of primary cells from three CLL patients. (C,D) The quantification analysis of protein bands in (A,B) by ImageJ software, a statistical graph showing the value divided by the internal reference protein β-actin, and compared with the control group.

Article Snippet: APG-115 was obtained from Suzhou Ascentage Pharma Co., Ltd. ABT-199 was a product of MCE.

Techniques: Activation Assay, Protein-Protein interactions, Expressing, Software, Control

APG-115 synergizes ABT-199 to induce cell apoptosis in CLL (A,B) Cell apoptosis was detected by flow cytometry in CLL patient primary cells, and (C) EHEB cells that were treated with described concentrations of APG-115 and ABT-199 for 48 h. The figure shows the representatives of three independent experiments, and the results are expressed as mean ± standard deviation. (D) The synergic effect was evaluated using the Compusyn software. The criteria for the Combination index (CI) value are as follows: <0.1, Very strong synergism; 0.1–0.3, Strong synergism; 0.3–0.7, Synergism; 0.7–0.85, Moderate synergism; 0.85–0.9, Slight synergism; 0.90–1.1, Nearly additive; >1.1 antagonism. In green, shows synergistic combinations. In red, shows additive combinations. In blue, shows average synergy in all combinations. All concentrations are in μM. (E) The effect of combination of APG-115 and ABT-119 on BCL-2, BCL-xL, and MCL-1 expression in EHEB cells. The data shown are representative images of three independent experiments. (F) The quantification analysis of protein bands in (E) by ImageJ software, the graph shows the value divided by the internal reference β-actin and compared with the control group without treatment. Statistical significance was determined using two-way ANOVA analysis with the Geisser-Greenhouse correction. A p -value of < 0.05 was considered statistically significant.* p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: Frontiers in Pharmacology

Article Title: MDM2 inhibitor APG-115 synergizes with ABT-199 to induce cell apoptosis in chronic lymphocytic leukemia

doi: 10.3389/fphar.2024.1441383

Figure Lengend Snippet: APG-115 synergizes ABT-199 to induce cell apoptosis in CLL (A,B) Cell apoptosis was detected by flow cytometry in CLL patient primary cells, and (C) EHEB cells that were treated with described concentrations of APG-115 and ABT-199 for 48 h. The figure shows the representatives of three independent experiments, and the results are expressed as mean ± standard deviation. (D) The synergic effect was evaluated using the Compusyn software. The criteria for the Combination index (CI) value are as follows: <0.1, Very strong synergism; 0.1–0.3, Strong synergism; 0.3–0.7, Synergism; 0.7–0.85, Moderate synergism; 0.85–0.9, Slight synergism; 0.90–1.1, Nearly additive; >1.1 antagonism. In green, shows synergistic combinations. In red, shows additive combinations. In blue, shows average synergy in all combinations. All concentrations are in μM. (E) The effect of combination of APG-115 and ABT-119 on BCL-2, BCL-xL, and MCL-1 expression in EHEB cells. The data shown are representative images of three independent experiments. (F) The quantification analysis of protein bands in (E) by ImageJ software, the graph shows the value divided by the internal reference β-actin and compared with the control group without treatment. Statistical significance was determined using two-way ANOVA analysis with the Geisser-Greenhouse correction. A p -value of < 0.05 was considered statistically significant.* p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: APG-115 was obtained from Suzhou Ascentage Pharma Co., Ltd. ABT-199 was a product of MCE.

Techniques: Flow Cytometry, Standard Deviation, Software, Expressing, Control

The proposed pro-apoptotic mechanism of APG-115 in CLL.

Journal: Frontiers in Pharmacology

Article Title: MDM2 inhibitor APG-115 synergizes with ABT-199 to induce cell apoptosis in chronic lymphocytic leukemia

doi: 10.3389/fphar.2024.1441383

Figure Lengend Snippet: The proposed pro-apoptotic mechanism of APG-115 in CLL.

Article Snippet: APG-115 was obtained from Suzhou Ascentage Pharma Co., Ltd. ABT-199 was a product of MCE.

Techniques: