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Image Search Results
Journal: The Journal of Neuroscience
Article Title: NPY Induces Stress Resilience via Downregulation of I h in Principal Neurons of Rat Basolateral Amygdala
doi: 10.1523/JNEUROSCI.3528-17.2018
Figure Lengend Snippet: qRT-PCR measurements reveal downregulation of HCN1 expression in 4W NPY- versus 4W Veh-treated BLA. A, HCN1 mRNA expression in 2W and 4W BLA. HCN1 expression was significantly reduced in the BLA of NPY-treated animals at 4W (2-way ANOVA; treatment: F(1,19) = 4.592, p = 0.04; Time: F(1,19) = 1.348, p = 0.26; interaction: F(1,19) = 5.078, p = 0.03). B, HCN2 expression in 2W and 4W BLA (2-way ANOVA; treatment: F(1,19) = 0.229, p = 0.63; time: F(1,19) = 2.096, p = 0.164; interaction: F(1,19) = 0.345, p = 0.56). C, HCN3 mRNA in 2W and 4W BLA (2-way ANOVA; treatment: F(1,16) = 0.84, p = 0.84; time: F(1,16) = 3.59, p = 0.08; interaction: F(1,16) = 1.73, p = 0.21). D, HCN4 expression in 2W and 4W BLA. HCN4 expression was increased in 4W relative to 2W BLA in both Veh- and NPY-treated animals, but was not affected by NPY treatment (2-way ANOVA; treatment: F(1,15) = 1.24, p = 0.28; time: F(1,15) = 25.26, p = 0.0002; interaction: F(1,15) = 0.0083, p = 0.93). Data are shown as mean ± SEM; numbers of individual animals studied are indicated in each bar. *p < 0.05, **p < 0.01, Bonferroni's multiple-comparisons test; significantly different from 2W time point (Fig. 8A) or corresponding 2W group (Fig. 8D).
Article Snippet: The primary antibodies used were guinea pig guinea pig anti-HCN1 (1:2000; Alomone Laboratories, catalog #AGP-203), rabbit anti-HCN2 (1:1000; Alomone Laboratories, catalog #APC-030, RRID: AB_2313726 ),
Techniques: Quantitative RT-PCR, Expressing