antitomm20 Search Results


anti tomm20  (Atlas Antibodies)
93
Atlas Antibodies anti tomm20
Anti Tomm20, supplied by Atlas Antibodies, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antitomm20/Anti-TOMM20/pmc06182735-38-4-9
Average 93 stars, based on 1 article reviews
anti tomm20 - by Bioz Stars, 2026-10
93/100 stars
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rabbit anti-tomm20  (Becton Dickinson)
90
Becton Dickinson rabbit anti-tomm20
a – c RT-qPCR analysis of si CTRL and si ATG5 LEC treated with sodium acetate (AC, 20 mM, 48 h) or vehicle. mRNA expression of Prox1, VEGFR3, and LYVE1 (relative to HPRT). Mean ± SD, N = 3 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, ** p < 0.01 and *** p < 0.001 vs si CTRL, ## p < 0.01 and ### p < 0.001 vs si ATG5. d Representative blots for the indicated proteins in si CTRL or si ATG5 LEC treated with AC or vehicle. Densitometric quantification is indicated beneath the blots. Mean ± SD, N ≥ 3 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, * p < 0.05,*** p < 0.001. e Representative blots for indicated proteins in si CTRL and si ATG5 LEC treated with palmitate (PAL, 500 nM, 48 h) or BSA. Densitometric quantification is indicated beneath the blots. Mean ± SD, N ≥ 3 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001. f Representative immunofluorescent images of si CTRL and si ATG5 treated with AC or vehicle and stained for mitochondrial protein <t>TOMM20</t> and BODIPY 493/503. Nuclei are stained with DAPI. Scale bars represent 10 µm. g Quantification of mitochondrial fragmentation index (number of mitochondria/ total mitochondrial area). Mean ± SD, N = 3 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, * p < 0.05 vs si CTRL, # p < 0.05 vs si ATG5. Mean represents mean per independent experiment, with a minimum of 12 cells analyzed per condition per independent experiment. h Quantification of lipid droplet number per cell. Mean ± SD, N = 4 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, * p < 0.05 vs si CTRL. Mean represents mean per independent experiment, with a minimum of 29 cells analyzed per condition per independent experiment. i Representative super resolution AiryScan images of si CTRL and si ATG5, si CTRL + si DRP1, and si ATG5 + si DRP1 LEC stained for the mitochondrial protein TOMM20 and BODIPY 493/503. Nuclei are stained with DAPI. Scale bars represent 10 µm. j Quantification of mitochondrial index of fragmentation (number of mitochondria/ total mitochondrial area). Mean ± SD, N = 3 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, **** p < 0.0001 vs si CTRL, #### p < 0.0001 vs si ATG5. Mean represents mean per independent experiment, with a minimum of 35 cells analyzed per condition per independent experiment. k Quantification of lipid droplet number per cell. Mean ± SD, N = 3 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, ** p < 0.01 vs si CTRL. Mean represents mean per independent experiment, with a minimum of 47 cells analyzed per condition per independent experiment. l – o RT-qPCR analysis in si CTRL, si ATG5, si CTRL + si DRP1 and si ATG5 + si DRP1 LEC. mRNA expression of PROX1, VEGFR3, LYVE1, and CPT1A (relative to HPRT). Mean ± SD, N = 3 ( l , n , o ) and N = 4 ( m ) biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, ** p < 0.01 vs si CTRL and p = ns vs si ATG5.
Rabbit Anti Tomm20, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antitomm20/anti+tomm20/pmc09120506-277-96-100
Average 90 stars, based on 1 article reviews
rabbit anti-tomm20 - by Bioz Stars, 2026-10
90/100 stars
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anti-tomm20  (ZenBio)
90
ZenBio anti-tomm20
a – c RT-qPCR analysis of si CTRL and si ATG5 LEC treated with sodium acetate (AC, 20 mM, 48 h) or vehicle. mRNA expression of Prox1, VEGFR3, and LYVE1 (relative to HPRT). Mean ± SD, N = 3 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, ** p < 0.01 and *** p < 0.001 vs si CTRL, ## p < 0.01 and ### p < 0.001 vs si ATG5. d Representative blots for the indicated proteins in si CTRL or si ATG5 LEC treated with AC or vehicle. Densitometric quantification is indicated beneath the blots. Mean ± SD, N ≥ 3 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, * p < 0.05,*** p < 0.001. e Representative blots for indicated proteins in si CTRL and si ATG5 LEC treated with palmitate (PAL, 500 nM, 48 h) or BSA. Densitometric quantification is indicated beneath the blots. Mean ± SD, N ≥ 3 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001. f Representative immunofluorescent images of si CTRL and si ATG5 treated with AC or vehicle and stained for mitochondrial protein <t>TOMM20</t> and BODIPY 493/503. Nuclei are stained with DAPI. Scale bars represent 10 µm. g Quantification of mitochondrial fragmentation index (number of mitochondria/ total mitochondrial area). Mean ± SD, N = 3 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, * p < 0.05 vs si CTRL, # p < 0.05 vs si ATG5. Mean represents mean per independent experiment, with a minimum of 12 cells analyzed per condition per independent experiment. h Quantification of lipid droplet number per cell. Mean ± SD, N = 4 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, * p < 0.05 vs si CTRL. Mean represents mean per independent experiment, with a minimum of 29 cells analyzed per condition per independent experiment. i Representative super resolution AiryScan images of si CTRL and si ATG5, si CTRL + si DRP1, and si ATG5 + si DRP1 LEC stained for the mitochondrial protein TOMM20 and BODIPY 493/503. Nuclei are stained with DAPI. Scale bars represent 10 µm. j Quantification of mitochondrial index of fragmentation (number of mitochondria/ total mitochondrial area). Mean ± SD, N = 3 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, **** p < 0.0001 vs si CTRL, #### p < 0.0001 vs si ATG5. Mean represents mean per independent experiment, with a minimum of 35 cells analyzed per condition per independent experiment. k Quantification of lipid droplet number per cell. Mean ± SD, N = 3 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, ** p < 0.01 vs si CTRL. Mean represents mean per independent experiment, with a minimum of 47 cells analyzed per condition per independent experiment. l – o RT-qPCR analysis in si CTRL, si ATG5, si CTRL + si DRP1 and si ATG5 + si DRP1 LEC. mRNA expression of PROX1, VEGFR3, LYVE1, and CPT1A (relative to HPRT). Mean ± SD, N = 3 ( l , n , o ) and N = 4 ( m ) biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, ** p < 0.01 vs si CTRL and p = ns vs si ATG5.
Anti Tomm20, supplied by ZenBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antitomm20/anti+tomm20/pm39840526-281-76-78
Average 90 stars, based on 1 article reviews
anti-tomm20 - by Bioz Stars, 2026-10
90/100 stars
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rabbit anti-tomm20 hpa011562  (Merck KGaA)
90
Merck KGaA rabbit anti-tomm20 hpa011562
a – c RT-qPCR analysis of si CTRL and si ATG5 LEC treated with sodium acetate (AC, 20 mM, 48 h) or vehicle. mRNA expression of Prox1, VEGFR3, and LYVE1 (relative to HPRT). Mean ± SD, N = 3 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, ** p < 0.01 and *** p < 0.001 vs si CTRL, ## p < 0.01 and ### p < 0.001 vs si ATG5. d Representative blots for the indicated proteins in si CTRL or si ATG5 LEC treated with AC or vehicle. Densitometric quantification is indicated beneath the blots. Mean ± SD, N ≥ 3 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, * p < 0.05,*** p < 0.001. e Representative blots for indicated proteins in si CTRL and si ATG5 LEC treated with palmitate (PAL, 500 nM, 48 h) or BSA. Densitometric quantification is indicated beneath the blots. Mean ± SD, N ≥ 3 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001. f Representative immunofluorescent images of si CTRL and si ATG5 treated with AC or vehicle and stained for mitochondrial protein <t>TOMM20</t> and BODIPY 493/503. Nuclei are stained with DAPI. Scale bars represent 10 µm. g Quantification of mitochondrial fragmentation index (number of mitochondria/ total mitochondrial area). Mean ± SD, N = 3 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, * p < 0.05 vs si CTRL, # p < 0.05 vs si ATG5. Mean represents mean per independent experiment, with a minimum of 12 cells analyzed per condition per independent experiment. h Quantification of lipid droplet number per cell. Mean ± SD, N = 4 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, * p < 0.05 vs si CTRL. Mean represents mean per independent experiment, with a minimum of 29 cells analyzed per condition per independent experiment. i Representative super resolution AiryScan images of si CTRL and si ATG5, si CTRL + si DRP1, and si ATG5 + si DRP1 LEC stained for the mitochondrial protein TOMM20 and BODIPY 493/503. Nuclei are stained with DAPI. Scale bars represent 10 µm. j Quantification of mitochondrial index of fragmentation (number of mitochondria/ total mitochondrial area). Mean ± SD, N = 3 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, **** p < 0.0001 vs si CTRL, #### p < 0.0001 vs si ATG5. Mean represents mean per independent experiment, with a minimum of 35 cells analyzed per condition per independent experiment. k Quantification of lipid droplet number per cell. Mean ± SD, N = 3 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, ** p < 0.01 vs si CTRL. Mean represents mean per independent experiment, with a minimum of 47 cells analyzed per condition per independent experiment. l – o RT-qPCR analysis in si CTRL, si ATG5, si CTRL + si DRP1 and si ATG5 + si DRP1 LEC. mRNA expression of PROX1, VEGFR3, LYVE1, and CPT1A (relative to HPRT). Mean ± SD, N = 3 ( l , n , o ) and N = 4 ( m ) biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, ** p < 0.01 vs si CTRL and p = ns vs si ATG5.
Rabbit Anti Tomm20 Hpa011562, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antitomm20/rabbit+anti+tomm20+hpa011562/pm39251668-263-121-124
Average 90 stars, based on 1 article reviews
rabbit anti-tomm20 hpa011562 - by Bioz Stars, 2026-10
90/100 stars
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tomm20 antibody  (Bio-Techne corporation)
94
Bio-Techne corporation tomm20 antibody
a – c RT-qPCR analysis of si CTRL and si ATG5 LEC treated with sodium acetate (AC, 20 mM, 48 h) or vehicle. mRNA expression of Prox1, VEGFR3, and LYVE1 (relative to HPRT). Mean ± SD, N = 3 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, ** p < 0.01 and *** p < 0.001 vs si CTRL, ## p < 0.01 and ### p < 0.001 vs si ATG5. d Representative blots for the indicated proteins in si CTRL or si ATG5 LEC treated with AC or vehicle. Densitometric quantification is indicated beneath the blots. Mean ± SD, N ≥ 3 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, * p < 0.05,*** p < 0.001. e Representative blots for indicated proteins in si CTRL and si ATG5 LEC treated with palmitate (PAL, 500 nM, 48 h) or BSA. Densitometric quantification is indicated beneath the blots. Mean ± SD, N ≥ 3 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001. f Representative immunofluorescent images of si CTRL and si ATG5 treated with AC or vehicle and stained for mitochondrial protein <t>TOMM20</t> and BODIPY 493/503. Nuclei are stained with DAPI. Scale bars represent 10 µm. g Quantification of mitochondrial fragmentation index (number of mitochondria/ total mitochondrial area). Mean ± SD, N = 3 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, * p < 0.05 vs si CTRL, # p < 0.05 vs si ATG5. Mean represents mean per independent experiment, with a minimum of 12 cells analyzed per condition per independent experiment. h Quantification of lipid droplet number per cell. Mean ± SD, N = 4 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, * p < 0.05 vs si CTRL. Mean represents mean per independent experiment, with a minimum of 29 cells analyzed per condition per independent experiment. i Representative super resolution AiryScan images of si CTRL and si ATG5, si CTRL + si DRP1, and si ATG5 + si DRP1 LEC stained for the mitochondrial protein TOMM20 and BODIPY 493/503. Nuclei are stained with DAPI. Scale bars represent 10 µm. j Quantification of mitochondrial index of fragmentation (number of mitochondria/ total mitochondrial area). Mean ± SD, N = 3 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, **** p < 0.0001 vs si CTRL, #### p < 0.0001 vs si ATG5. Mean represents mean per independent experiment, with a minimum of 35 cells analyzed per condition per independent experiment. k Quantification of lipid droplet number per cell. Mean ± SD, N = 3 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, ** p < 0.01 vs si CTRL. Mean represents mean per independent experiment, with a minimum of 47 cells analyzed per condition per independent experiment. l – o RT-qPCR analysis in si CTRL, si ATG5, si CTRL + si DRP1 and si ATG5 + si DRP1 LEC. mRNA expression of PROX1, VEGFR3, LYVE1, and CPT1A (relative to HPRT). Mean ± SD, N = 3 ( l , n , o ) and N = 4 ( m ) biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, ** p < 0.01 vs si CTRL and p = ns vs si ATG5.
Tomm20 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antitomm20/TOMM20+Antibody/custom%40nbp1-81556%4030699348
Average 94 stars, based on 1 article reviews
tomm20 antibody - by Bioz Stars, 2026-10
94/100 stars
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tomm20  (Merck & Co)
86
Merck & Co tomm20
a – c RT-qPCR analysis of si CTRL and si ATG5 LEC treated with sodium acetate (AC, 20 mM, 48 h) or vehicle. mRNA expression of Prox1, VEGFR3, and LYVE1 (relative to HPRT). Mean ± SD, N = 3 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, ** p < 0.01 and *** p < 0.001 vs si CTRL, ## p < 0.01 and ### p < 0.001 vs si ATG5. d Representative blots for the indicated proteins in si CTRL or si ATG5 LEC treated with AC or vehicle. Densitometric quantification is indicated beneath the blots. Mean ± SD, N ≥ 3 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, * p < 0.05,*** p < 0.001. e Representative blots for indicated proteins in si CTRL and si ATG5 LEC treated with palmitate (PAL, 500 nM, 48 h) or BSA. Densitometric quantification is indicated beneath the blots. Mean ± SD, N ≥ 3 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001. f Representative immunofluorescent images of si CTRL and si ATG5 treated with AC or vehicle and stained for mitochondrial protein <t>TOMM20</t> and BODIPY 493/503. Nuclei are stained with DAPI. Scale bars represent 10 µm. g Quantification of mitochondrial fragmentation index (number of mitochondria/ total mitochondrial area). Mean ± SD, N = 3 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, * p < 0.05 vs si CTRL, # p < 0.05 vs si ATG5. Mean represents mean per independent experiment, with a minimum of 12 cells analyzed per condition per independent experiment. h Quantification of lipid droplet number per cell. Mean ± SD, N = 4 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, * p < 0.05 vs si CTRL. Mean represents mean per independent experiment, with a minimum of 29 cells analyzed per condition per independent experiment. i Representative super resolution AiryScan images of si CTRL and si ATG5, si CTRL + si DRP1, and si ATG5 + si DRP1 LEC stained for the mitochondrial protein TOMM20 and BODIPY 493/503. Nuclei are stained with DAPI. Scale bars represent 10 µm. j Quantification of mitochondrial index of fragmentation (number of mitochondria/ total mitochondrial area). Mean ± SD, N = 3 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, **** p < 0.0001 vs si CTRL, #### p < 0.0001 vs si ATG5. Mean represents mean per independent experiment, with a minimum of 35 cells analyzed per condition per independent experiment. k Quantification of lipid droplet number per cell. Mean ± SD, N = 3 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, ** p < 0.01 vs si CTRL. Mean represents mean per independent experiment, with a minimum of 47 cells analyzed per condition per independent experiment. l – o RT-qPCR analysis in si CTRL, si ATG5, si CTRL + si DRP1 and si ATG5 + si DRP1 LEC. mRNA expression of PROX1, VEGFR3, LYVE1, and CPT1A (relative to HPRT). Mean ± SD, N = 3 ( l , n , o ) and N = 4 ( m ) biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, ** p < 0.01 vs si CTRL and p = ns vs si ATG5.
Tomm20, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antitomm20/anti+tomm20/pmc12410566-530-45-46
Average 86 stars, based on 1 article reviews
tomm20 - by Bioz Stars, 2026-10
86/100 stars
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anti tom20 antibody  (Huabio Inc)
86
Huabio Inc anti tom20 antibody
AGO-2 expression is elevated in DCM but its mitochondrial localization is reduced. (A–C) Quantification of AGO-2, ETC I and III, and 8-OHdG levels in the hearts of Lepr db/db mice at different ages. (D) immunofluorescence staining of AGO-2 and <t>TOM20</t> (mitochondrial marker) in cardiac tissue. (E) TEM images showing ultrastructural changes in myocardial mitochondria of control and Lepr db/db mice. (F) Myocardial 8-OHdG staining in control and Lepr db/db mice.
Anti Tom20 Antibody, supplied by Huabio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antitomm20/anti+tomm20/pmc12874382-80-14-17
Average 86 stars, based on 1 article reviews
anti tom20 antibody - by Bioz Stars, 2026-10
86/100 stars
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antitomm 20  (Abnova)
90
Abnova antitomm 20
AGO-2 expression is elevated in DCM but its mitochondrial localization is reduced. (A–C) Quantification of AGO-2, ETC I and III, and 8-OHdG levels in the hearts of Lepr db/db mice at different ages. (D) immunofluorescence staining of AGO-2 and <t>TOM20</t> (mitochondrial marker) in cardiac tissue. (E) TEM images showing ultrastructural changes in myocardial mitochondria of control and Lepr db/db mice. (F) Myocardial 8-OHdG staining in control and Lepr db/db mice.
Antitomm 20, supplied by Abnova, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antitomm20/antitomm+20/pm21545838-174-12-15
Average 90 stars, based on 1 article reviews
antitomm 20 - by Bioz Stars, 2026-10
90/100 stars
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tomm20  (Servicebio Inc)
86
Servicebio Inc tomm20
Effects of PQQ treatment on mitochondrial damage induced by ENR exposure in IEC‐6 cells. (A) Experimental design diagram. (B) Network diagram depicting mitochondrial‐related genes and associated biological pathways. (C–M) Differentially expressed genes related to mitochondrial function. (N–O) Protein levels of <t>Tomm20</t> measured by Western blot analysis. (P) OCR was recorded at baseline and after the sequential injection of oligomycin, FCCP, and a mixture of rotenone and antimycin A. (Q–T) Quantitative analysis of mitochondrial respiration parameters: Basal respiration (Q), ATP‐linked respiration (R), proton leak (S), and maximal respiration (T). Statistical significance among the Control, ENR, and ENR + PQQ groups was assessed using one‐way ANOVA followed by Tukey's multiple‐comparisons test. * p < 0.05, ** p < 0.01, *** p < 0.001.
Tomm20, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antitomm20/anti+tomm20/pmc13130356-84-31-22
Average 86 stars, based on 1 article reviews
tomm20 - by Bioz Stars, 2026-10
86/100 stars
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c fos  (Abmart Inc)
86
Abmart Inc c fos
AP-1 <t>(c-FOS</t> and c-JUN) is a key regulator of PCI-34051 in the regulation of HASMC ferroptosis. (A) Volcano plot displaying the gene expression distribution and differentially expressed genes among the DMSO and PCI-34051 groups after treatment with CD. (B) Gene Ontology (GO) enrichment analysis showed that biological processes and cellular components were associated with these differentially expressed genes. (C, D) Western-blot analysis and quantification showing the protein levels of c-FOS and c-JUN in HASMCs after treatment with DMSO and PCI-34051 under the ferroptosis models induced by <t>CD.</t> <t>β-Actin</t> served as a loading control ( n = 4 per group). (E, F) Co-immunoprecipitation results showed that HDAC8 interacted with c-JUN in HASMCs. (G) Results of GST pull-down demonstrated a direct interaction between HDAC8 and c-JUN in HASMCs. Values are means ± SD; *** P < 0.001. BP, biological process. CC, cellular component. MF, molecular function.
C Fos, supplied by Abmart Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antitomm20/anti+tomm20/pmc13250666-245-19-21
Average 86 stars, based on 1 article reviews
c fos - by Bioz Stars, 2026-10
86/100 stars
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anti tomm20  (Progen Biotechnik)
86
Progen Biotechnik anti tomm20
AP-1 <t>(c-FOS</t> and c-JUN) is a key regulator of PCI-34051 in the regulation of HASMC ferroptosis. (A) Volcano plot displaying the gene expression distribution and differentially expressed genes among the DMSO and PCI-34051 groups after treatment with CD. (B) Gene Ontology (GO) enrichment analysis showed that biological processes and cellular components were associated with these differentially expressed genes. (C, D) Western-blot analysis and quantification showing the protein levels of c-FOS and c-JUN in HASMCs after treatment with DMSO and PCI-34051 under the ferroptosis models induced by <t>CD.</t> <t>β-Actin</t> served as a loading control ( n = 4 per group). (E, F) Co-immunoprecipitation results showed that HDAC8 interacted with c-JUN in HASMCs. (G) Results of GST pull-down demonstrated a direct interaction between HDAC8 and c-JUN in HASMCs. Values are means ± SD; *** P < 0.001. BP, biological process. CC, cellular component. MF, molecular function.
Anti Tomm20, supplied by Progen Biotechnik, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antitomm20/anti+tomm20/pm37906411-150-31-28
Average 86 stars, based on 1 article reviews
anti tomm20 - by Bioz Stars, 2026-10
86/100 stars
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anti-tomm20 antibody  (Antibodies Inc)
99
Antibodies Inc anti-tomm20 antibody
AP-1 <t>(c-FOS</t> and c-JUN) is a key regulator of PCI-34051 in the regulation of HASMC ferroptosis. (A) Volcano plot displaying the gene expression distribution and differentially expressed genes among the DMSO and PCI-34051 groups after treatment with CD. (B) Gene Ontology (GO) enrichment analysis showed that biological processes and cellular components were associated with these differentially expressed genes. (C, D) Western-blot analysis and quantification showing the protein levels of c-FOS and c-JUN in HASMCs after treatment with DMSO and PCI-34051 under the ferroptosis models induced by <t>CD.</t> <t>β-Actin</t> served as a loading control ( n = 4 per group). (E, F) Co-immunoprecipitation results showed that HDAC8 interacted with c-JUN in HASMCs. (G) Results of GST pull-down demonstrated a direct interaction between HDAC8 and c-JUN in HASMCs. Values are means ± SD; *** P < 0.001. BP, biological process. CC, cellular component. MF, molecular function.
Anti Tomm20 Antibody, supplied by Antibodies Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antitomm20/Anti-TOMM20+Antibody/custom%40tomm20-0100%40pmc12882647__mmc1
Average 99 stars, based on 1 article reviews
anti-tomm20 antibody - by Bioz Stars, 2026-10
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Image Search Results


a – c RT-qPCR analysis of si CTRL and si ATG5 LEC treated with sodium acetate (AC, 20 mM, 48 h) or vehicle. mRNA expression of Prox1, VEGFR3, and LYVE1 (relative to HPRT). Mean ± SD, N = 3 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, ** p < 0.01 and *** p < 0.001 vs si CTRL, ## p < 0.01 and ### p < 0.001 vs si ATG5. d Representative blots for the indicated proteins in si CTRL or si ATG5 LEC treated with AC or vehicle. Densitometric quantification is indicated beneath the blots. Mean ± SD, N ≥ 3 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, * p < 0.05,*** p < 0.001. e Representative blots for indicated proteins in si CTRL and si ATG5 LEC treated with palmitate (PAL, 500 nM, 48 h) or BSA. Densitometric quantification is indicated beneath the blots. Mean ± SD, N ≥ 3 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001. f Representative immunofluorescent images of si CTRL and si ATG5 treated with AC or vehicle and stained for mitochondrial protein TOMM20 and BODIPY 493/503. Nuclei are stained with DAPI. Scale bars represent 10 µm. g Quantification of mitochondrial fragmentation index (number of mitochondria/ total mitochondrial area). Mean ± SD, N = 3 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, * p < 0.05 vs si CTRL, # p < 0.05 vs si ATG5. Mean represents mean per independent experiment, with a minimum of 12 cells analyzed per condition per independent experiment. h Quantification of lipid droplet number per cell. Mean ± SD, N = 4 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, * p < 0.05 vs si CTRL. Mean represents mean per independent experiment, with a minimum of 29 cells analyzed per condition per independent experiment. i Representative super resolution AiryScan images of si CTRL and si ATG5, si CTRL + si DRP1, and si ATG5 + si DRP1 LEC stained for the mitochondrial protein TOMM20 and BODIPY 493/503. Nuclei are stained with DAPI. Scale bars represent 10 µm. j Quantification of mitochondrial index of fragmentation (number of mitochondria/ total mitochondrial area). Mean ± SD, N = 3 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, **** p < 0.0001 vs si CTRL, #### p < 0.0001 vs si ATG5. Mean represents mean per independent experiment, with a minimum of 35 cells analyzed per condition per independent experiment. k Quantification of lipid droplet number per cell. Mean ± SD, N = 3 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, ** p < 0.01 vs si CTRL. Mean represents mean per independent experiment, with a minimum of 47 cells analyzed per condition per independent experiment. l – o RT-qPCR analysis in si CTRL, si ATG5, si CTRL + si DRP1 and si ATG5 + si DRP1 LEC. mRNA expression of PROX1, VEGFR3, LYVE1, and CPT1A (relative to HPRT). Mean ± SD, N = 3 ( l , n , o ) and N = 4 ( m ) biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, ** p < 0.01 vs si CTRL and p = ns vs si ATG5.

Journal: Nature Communications

Article Title: Lipid droplet degradation by autophagy connects mitochondria metabolism to Prox1-driven expression of lymphatic genes and lymphangiogenesis

doi: 10.1038/s41467-022-30490-6

Figure Lengend Snippet: a – c RT-qPCR analysis of si CTRL and si ATG5 LEC treated with sodium acetate (AC, 20 mM, 48 h) or vehicle. mRNA expression of Prox1, VEGFR3, and LYVE1 (relative to HPRT). Mean ± SD, N = 3 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, ** p < 0.01 and *** p < 0.001 vs si CTRL, ## p < 0.01 and ### p < 0.001 vs si ATG5. d Representative blots for the indicated proteins in si CTRL or si ATG5 LEC treated with AC or vehicle. Densitometric quantification is indicated beneath the blots. Mean ± SD, N ≥ 3 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, * p < 0.05,*** p < 0.001. e Representative blots for indicated proteins in si CTRL and si ATG5 LEC treated with palmitate (PAL, 500 nM, 48 h) or BSA. Densitometric quantification is indicated beneath the blots. Mean ± SD, N ≥ 3 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001. f Representative immunofluorescent images of si CTRL and si ATG5 treated with AC or vehicle and stained for mitochondrial protein TOMM20 and BODIPY 493/503. Nuclei are stained with DAPI. Scale bars represent 10 µm. g Quantification of mitochondrial fragmentation index (number of mitochondria/ total mitochondrial area). Mean ± SD, N = 3 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, * p < 0.05 vs si CTRL, # p < 0.05 vs si ATG5. Mean represents mean per independent experiment, with a minimum of 12 cells analyzed per condition per independent experiment. h Quantification of lipid droplet number per cell. Mean ± SD, N = 4 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, * p < 0.05 vs si CTRL. Mean represents mean per independent experiment, with a minimum of 29 cells analyzed per condition per independent experiment. i Representative super resolution AiryScan images of si CTRL and si ATG5, si CTRL + si DRP1, and si ATG5 + si DRP1 LEC stained for the mitochondrial protein TOMM20 and BODIPY 493/503. Nuclei are stained with DAPI. Scale bars represent 10 µm. j Quantification of mitochondrial index of fragmentation (number of mitochondria/ total mitochondrial area). Mean ± SD, N = 3 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, **** p < 0.0001 vs si CTRL, #### p < 0.0001 vs si ATG5. Mean represents mean per independent experiment, with a minimum of 35 cells analyzed per condition per independent experiment. k Quantification of lipid droplet number per cell. Mean ± SD, N = 3 biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, ** p < 0.01 vs si CTRL. Mean represents mean per independent experiment, with a minimum of 47 cells analyzed per condition per independent experiment. l – o RT-qPCR analysis in si CTRL, si ATG5, si CTRL + si DRP1 and si ATG5 + si DRP1 LEC. mRNA expression of PROX1, VEGFR3, LYVE1, and CPT1A (relative to HPRT). Mean ± SD, N = 3 ( l , n , o ) and N = 4 ( m ) biological replicates analyzed by one-way ANOVA, with Tukey’s test for multiple comparisons, ** p < 0.01 vs si CTRL and p = ns vs si ATG5.

Article Snippet: Primary antibodies used were rabbit anti-ATG5 (12994S, CST), rabbit anti-ATG7 (1:1000, 8558S, CST), rabbit anti-LC3 (1:1000, 3868S, CST), rabbit anti-GAPDH (1:5000, 2118S, CST), rabbit anti-p62 (1:1000, p0067, Millipore), rabbit anti-β-actin (1:5000, A5441, Sigma-Aldrich), goat anti-LYVE1 (1:1000, AF2089, R&D systems), rabbit anti-PROX1 (1:1000, 11067-2, Proteintech), rabbit anti-VEGFR3 (1:1000, ab154079, Abcam), mouse anti-NR2F2 (1:1000, ab41859, Abcam), rabbit anti-CPT1 (1:1000, D3B3, CST) antibody, rabbit anti-CPT2 (1:1000, ab18114, Abcam), rabbit anti-acetyl histone H3 (lysine 9) antibody (1:1000, 9671, CST), rabbit anti-pan-acetyl histone H3 antibody (1:1000, 39139, Active Motif)), mouse anti-CD36 antibody (1:1000; ab17044, Abcam), mouse anti-ULK1 antibody (1:1000; ab56344, Abcam), rabbit anti-TOMM20 (1:1000; BD612278, BD Biosciences), mouse anti-OPA1 (1:1000; 612607, BD Biosciences), mouse anti-ENOS (1:1000; 610297, BD biosciences), rabbit anti-phospho DRP1 (Ser616) (1: 1000; 3455S, CST), mouse anti-DRP1 (1:1000; 611113, BD Biosciences), rabbit anti-H3K4me antibody (1:1000; ab8580, Abcam), rabbit anti-H3K27 ac (1:1000; 8173T, CST) and rabbit anti-FABP4 antibody (1:1000; 2120S, CST).

Techniques: Quantitative RT-PCR, Expressing, Staining

AGO-2 expression is elevated in DCM but its mitochondrial localization is reduced. (A–C) Quantification of AGO-2, ETC I and III, and 8-OHdG levels in the hearts of Lepr db/db mice at different ages. (D) immunofluorescence staining of AGO-2 and TOM20 (mitochondrial marker) in cardiac tissue. (E) TEM images showing ultrastructural changes in myocardial mitochondria of control and Lepr db/db mice. (F) Myocardial 8-OHdG staining in control and Lepr db/db mice.

Journal: ACS Applied Materials & Interfaces

Article Title: Targeted Delivery of AGO‑2 to Myocardial Mitochondria via Functionalized Nanoparticles Attenuates Oxidative Stress in Diabetic Cardiomyopathy

doi: 10.1021/acsami.5c15154

Figure Lengend Snippet: AGO-2 expression is elevated in DCM but its mitochondrial localization is reduced. (A–C) Quantification of AGO-2, ETC I and III, and 8-OHdG levels in the hearts of Lepr db/db mice at different ages. (D) immunofluorescence staining of AGO-2 and TOM20 (mitochondrial marker) in cardiac tissue. (E) TEM images showing ultrastructural changes in myocardial mitochondria of control and Lepr db/db mice. (F) Myocardial 8-OHdG staining in control and Lepr db/db mice.

Article Snippet: Cells were then fixed with formaldehyde, permeabilized with Triton X-100, and stained with an anti-TOM20 antibody (ST04–72, HUABIO, China) to label mitochondria.

Techniques: Expressing, Immunofluorescence, Staining, Marker, Control

ESC-AGO-2-mediated mitochondrial targeting in diabetic myocardium. (A) Immunofluorescence staining of Lepr db/db mouse hearts after treatment with PBS, ESC, or ESC-AGO-2 (20 μg) for 2 weeks. TOM20 (green) indicates mitochondrial localization, while CHP within ESC exhibits intrinsic red fluorescence. (B) TEM results of these mouse hearts, indicating possible localization of ESC in mitochondria. The arrows indicate the ESIO nanoparticles. Mt: mitochondira; lyso: lysosome. (C) Immunofluorescence staining the mouse hearts show the colocalization of ESC, mitochondria and AGO-2. TOM20 show cyan fluorescence, while ESCs emitted red fluorescence; nuclei were counterstained with DAPI, and AGO-2 protein was labeled with anti-AGO-2 (green fluorescence).

Journal: ACS Applied Materials & Interfaces

Article Title: Targeted Delivery of AGO‑2 to Myocardial Mitochondria via Functionalized Nanoparticles Attenuates Oxidative Stress in Diabetic Cardiomyopathy

doi: 10.1021/acsami.5c15154

Figure Lengend Snippet: ESC-AGO-2-mediated mitochondrial targeting in diabetic myocardium. (A) Immunofluorescence staining of Lepr db/db mouse hearts after treatment with PBS, ESC, or ESC-AGO-2 (20 μg) for 2 weeks. TOM20 (green) indicates mitochondrial localization, while CHP within ESC exhibits intrinsic red fluorescence. (B) TEM results of these mouse hearts, indicating possible localization of ESC in mitochondria. The arrows indicate the ESIO nanoparticles. Mt: mitochondira; lyso: lysosome. (C) Immunofluorescence staining the mouse hearts show the colocalization of ESC, mitochondria and AGO-2. TOM20 show cyan fluorescence, while ESCs emitted red fluorescence; nuclei were counterstained with DAPI, and AGO-2 protein was labeled with anti-AGO-2 (green fluorescence).

Article Snippet: Cells were then fixed with formaldehyde, permeabilized with Triton X-100, and stained with an anti-TOM20 antibody (ST04–72, HUABIO, China) to label mitochondria.

Techniques: Immunofluorescence, Staining, Fluorescence, Labeling

Effects of PQQ treatment on mitochondrial damage induced by ENR exposure in IEC‐6 cells. (A) Experimental design diagram. (B) Network diagram depicting mitochondrial‐related genes and associated biological pathways. (C–M) Differentially expressed genes related to mitochondrial function. (N–O) Protein levels of Tomm20 measured by Western blot analysis. (P) OCR was recorded at baseline and after the sequential injection of oligomycin, FCCP, and a mixture of rotenone and antimycin A. (Q–T) Quantitative analysis of mitochondrial respiration parameters: Basal respiration (Q), ATP‐linked respiration (R), proton leak (S), and maximal respiration (T). Statistical significance among the Control, ENR, and ENR + PQQ groups was assessed using one‐way ANOVA followed by Tukey's multiple‐comparisons test. * p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: Aging Cell

Article Title: Environmental Enrofloxacin Exposure as a Modifiable Driver of Mitochondria‐Mediated Intestinal Aging and Barrier Dysfunction

doi: 10.1111/acel.70526

Figure Lengend Snippet: Effects of PQQ treatment on mitochondrial damage induced by ENR exposure in IEC‐6 cells. (A) Experimental design diagram. (B) Network diagram depicting mitochondrial‐related genes and associated biological pathways. (C–M) Differentially expressed genes related to mitochondrial function. (N–O) Protein levels of Tomm20 measured by Western blot analysis. (P) OCR was recorded at baseline and after the sequential injection of oligomycin, FCCP, and a mixture of rotenone and antimycin A. (Q–T) Quantitative analysis of mitochondrial respiration parameters: Basal respiration (Q), ATP‐linked respiration (R), proton leak (S), and maximal respiration (T). Statistical significance among the Control, ENR, and ENR + PQQ groups was assessed using one‐way ANOVA followed by Tukey's multiple‐comparisons test. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Intestinal tissues were fixed (4% paraformaldehyde, 15 min), blocked (10% goat serum albumin, 0.4% Triton X‐100), and incubated with primary antibodies from Servicebio: Cdkn1a (GB11153, 1:300), Cdkn2a ( GB151143 , 1:300), Tomm20 ( GB151481 , 1:1000), Grp75 ( GB112273 , 1:650), Cox5a ( GB111676 , 1:500), Hsp60 ( GB112464 , 1:800), Cox4 (GB11250, 1:200), CD3 (GB13014, 1:100), Mucin‐2 (GB11344, 1:500), Occludin ( GB111401 , 1:750), Zo‐1 ( GB115686 , 1:1000), and Claudin‐1 ( GB112543 , 1:1000).

Techniques: Western Blot, Injection, Control

Effects of PQQ treatment on mitochondrial function, inflammation, and hypoxia levels in the intestine following ENR exposure. Experimental design diagram (A). Representative immunofluorescence images of mitochondrial function‐related proteins (Hsp60, Cox4, Tomm20, Grp75, Cox5a) and quantification (B–I). Representative immunofluorescence images of CD3 and quantification (J–K). Representative immunofluorescence images of hypoxia markers and quantification (L, M). Data are presented as the mean ± standard error of the mean. Statistical significance among the Control, ENR, and ENR + PQQ groups was assessed using one‐way ANOVA followed by Tukey's multiple‐comparisons test. * p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: Aging Cell

Article Title: Environmental Enrofloxacin Exposure as a Modifiable Driver of Mitochondria‐Mediated Intestinal Aging and Barrier Dysfunction

doi: 10.1111/acel.70526

Figure Lengend Snippet: Effects of PQQ treatment on mitochondrial function, inflammation, and hypoxia levels in the intestine following ENR exposure. Experimental design diagram (A). Representative immunofluorescence images of mitochondrial function‐related proteins (Hsp60, Cox4, Tomm20, Grp75, Cox5a) and quantification (B–I). Representative immunofluorescence images of CD3 and quantification (J–K). Representative immunofluorescence images of hypoxia markers and quantification (L, M). Data are presented as the mean ± standard error of the mean. Statistical significance among the Control, ENR, and ENR + PQQ groups was assessed using one‐way ANOVA followed by Tukey's multiple‐comparisons test. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Intestinal tissues were fixed (4% paraformaldehyde, 15 min), blocked (10% goat serum albumin, 0.4% Triton X‐100), and incubated with primary antibodies from Servicebio: Cdkn1a (GB11153, 1:300), Cdkn2a ( GB151143 , 1:300), Tomm20 ( GB151481 , 1:1000), Grp75 ( GB112273 , 1:650), Cox5a ( GB111676 , 1:500), Hsp60 ( GB112464 , 1:800), Cox4 (GB11250, 1:200), CD3 (GB13014, 1:100), Mucin‐2 (GB11344, 1:500), Occludin ( GB111401 , 1:750), Zo‐1 ( GB115686 , 1:1000), and Claudin‐1 ( GB112543 , 1:1000).

Techniques: Immunofluorescence, Control

AP-1 (c-FOS and c-JUN) is a key regulator of PCI-34051 in the regulation of HASMC ferroptosis. (A) Volcano plot displaying the gene expression distribution and differentially expressed genes among the DMSO and PCI-34051 groups after treatment with CD. (B) Gene Ontology (GO) enrichment analysis showed that biological processes and cellular components were associated with these differentially expressed genes. (C, D) Western-blot analysis and quantification showing the protein levels of c-FOS and c-JUN in HASMCs after treatment with DMSO and PCI-34051 under the ferroptosis models induced by CD. β-Actin served as a loading control ( n = 4 per group). (E, F) Co-immunoprecipitation results showed that HDAC8 interacted with c-JUN in HASMCs. (G) Results of GST pull-down demonstrated a direct interaction between HDAC8 and c-JUN in HASMCs. Values are means ± SD; *** P < 0.001. BP, biological process. CC, cellular component. MF, molecular function.

Journal: Life Medicine

Article Title: HDAC8-selective inhibitor PCI-34051 protects against aortic dissection by attenuating ferroptosis of vascular smooth muscle cells

doi: 10.1093/lifemedi/lnag013

Figure Lengend Snippet: AP-1 (c-FOS and c-JUN) is a key regulator of PCI-34051 in the regulation of HASMC ferroptosis. (A) Volcano plot displaying the gene expression distribution and differentially expressed genes among the DMSO and PCI-34051 groups after treatment with CD. (B) Gene Ontology (GO) enrichment analysis showed that biological processes and cellular components were associated with these differentially expressed genes. (C, D) Western-blot analysis and quantification showing the protein levels of c-FOS and c-JUN in HASMCs after treatment with DMSO and PCI-34051 under the ferroptosis models induced by CD. β-Actin served as a loading control ( n = 4 per group). (E, F) Co-immunoprecipitation results showed that HDAC8 interacted with c-JUN in HASMCs. (G) Results of GST pull-down demonstrated a direct interaction between HDAC8 and c-JUN in HASMCs. Values are means ± SD; *** P < 0.001. BP, biological process. CC, cellular component. MF, molecular function.

Article Snippet: The antibodies applied in this study were: β-Actin (AC026, ABclonal), FSP1 (20886-1-AP, Proteintech), GPX4 (ab125066, Abcam), c-JUN (T55290F, ABmart), c-FOS (T56596F, ABmart), Flag (F1804, Sigma-Aldrich), HDAC8 (17548-1-AP, Proteintech), SLC7A11 (26864-1-AP, Proteintech), 4-HNE (MAB3249-SP, Bio-techne), CD86 (13395-1-AP, Proteintech), α-SMA (ab7817, Abcam), α-SMA (GTX100034, Genetex), GST (AE001, Abclonal).

Techniques: Gene Expression, Western Blot, Control, Immunoprecipitation

AP-1 (c-FOS and c-JUN) eliminated the effects of PCI-34051 on HASMC ferroptosis. All HASMCs were infected with lenti-Flag and lenti-c-FOS + lenti-c-JUN, and then these HASMCs were used for subsequent experiments. (A, B) The protein level of AP-1 (c-JUN and c-FOS) was detected by Western blot in HASMCs infected with lenti-Flag and lenti-c-JUN + lenti-c-FOS ( n = 4 per group). (C, D) The CCK8 assay showing the relative viability of HASMCs treated with DMSO and PCI-34051 after CD (C) and IKE (D) stimulation for the indicated time ( n = 5 per group). (E, F) Flow cytometry with propidium iodide (PI) staining displaying the percentage of PI-positive cells of HASMCs after treatment as described above ( n = 4 per group). (G, H) The LDH assay indicating the relative cell damage rate of HASMCs treated with described above ( n = 5 per group). (I–L) The ratio of oxidized BODIPY-C11/non-oxidized BODIPY-C11 fluorescence revealing the level of ROS of HASMCs treated with described above ( n = 4 per group). (M–P). 4-HNE immunofluorescence staining and quantitative analysis exhibiting the content of 4-HNE in HASMCs after treatment as described above ( n = 4 per group). (Q–T) Western-blot analysis and quantification performed to assess the protein levels of GPX4, FSP1, and SLC7A11 in AP-1 overexpressed HASMCs after DMSO and PCI-34051 treatment along with CD or IKE induction. β-Actin served as a loading control ( n = 4 per group). Values are means ± SD; *** P < 0.001, ** P < 0.01, * P < 0.05.

Journal: Life Medicine

Article Title: HDAC8-selective inhibitor PCI-34051 protects against aortic dissection by attenuating ferroptosis of vascular smooth muscle cells

doi: 10.1093/lifemedi/lnag013

Figure Lengend Snippet: AP-1 (c-FOS and c-JUN) eliminated the effects of PCI-34051 on HASMC ferroptosis. All HASMCs were infected with lenti-Flag and lenti-c-FOS + lenti-c-JUN, and then these HASMCs were used for subsequent experiments. (A, B) The protein level of AP-1 (c-JUN and c-FOS) was detected by Western blot in HASMCs infected with lenti-Flag and lenti-c-JUN + lenti-c-FOS ( n = 4 per group). (C, D) The CCK8 assay showing the relative viability of HASMCs treated with DMSO and PCI-34051 after CD (C) and IKE (D) stimulation for the indicated time ( n = 5 per group). (E, F) Flow cytometry with propidium iodide (PI) staining displaying the percentage of PI-positive cells of HASMCs after treatment as described above ( n = 4 per group). (G, H) The LDH assay indicating the relative cell damage rate of HASMCs treated with described above ( n = 5 per group). (I–L) The ratio of oxidized BODIPY-C11/non-oxidized BODIPY-C11 fluorescence revealing the level of ROS of HASMCs treated with described above ( n = 4 per group). (M–P). 4-HNE immunofluorescence staining and quantitative analysis exhibiting the content of 4-HNE in HASMCs after treatment as described above ( n = 4 per group). (Q–T) Western-blot analysis and quantification performed to assess the protein levels of GPX4, FSP1, and SLC7A11 in AP-1 overexpressed HASMCs after DMSO and PCI-34051 treatment along with CD or IKE induction. β-Actin served as a loading control ( n = 4 per group). Values are means ± SD; *** P < 0.001, ** P < 0.01, * P < 0.05.

Article Snippet: The antibodies applied in this study were: β-Actin (AC026, ABclonal), FSP1 (20886-1-AP, Proteintech), GPX4 (ab125066, Abcam), c-JUN (T55290F, ABmart), c-FOS (T56596F, ABmart), Flag (F1804, Sigma-Aldrich), HDAC8 (17548-1-AP, Proteintech), SLC7A11 (26864-1-AP, Proteintech), 4-HNE (MAB3249-SP, Bio-techne), CD86 (13395-1-AP, Proteintech), α-SMA (ab7817, Abcam), α-SMA (GTX100034, Genetex), GST (AE001, Abclonal).

Techniques: Infection, Western Blot, CCK-8 Assay, Flow Cytometry, Staining, Lactate Dehydrogenase Assay, Fluorescence, Immunofluorescence, Control

The working model of PCI-34051 regulating HASMC ferroptosis and aortic dissection in mice. PCI-34051 inhibits the ferroptosis of HASMC by affecting the interaction between HDAC8 and AP-1 (c-FOS and c-JUN) and further suppresses the occurrence and development of BAPN-induced aortic dissection in mice. (This figure is created with Biorender).

Journal: Life Medicine

Article Title: HDAC8-selective inhibitor PCI-34051 protects against aortic dissection by attenuating ferroptosis of vascular smooth muscle cells

doi: 10.1093/lifemedi/lnag013

Figure Lengend Snippet: The working model of PCI-34051 regulating HASMC ferroptosis and aortic dissection in mice. PCI-34051 inhibits the ferroptosis of HASMC by affecting the interaction between HDAC8 and AP-1 (c-FOS and c-JUN) and further suppresses the occurrence and development of BAPN-induced aortic dissection in mice. (This figure is created with Biorender).

Article Snippet: The antibodies applied in this study were: β-Actin (AC026, ABclonal), FSP1 (20886-1-AP, Proteintech), GPX4 (ab125066, Abcam), c-JUN (T55290F, ABmart), c-FOS (T56596F, ABmart), Flag (F1804, Sigma-Aldrich), HDAC8 (17548-1-AP, Proteintech), SLC7A11 (26864-1-AP, Proteintech), 4-HNE (MAB3249-SP, Bio-techne), CD86 (13395-1-AP, Proteintech), α-SMA (ab7817, Abcam), α-SMA (GTX100034, Genetex), GST (AE001, Abclonal).

Techniques: Dissection