Journal: Neuroimmunomodulation

Article Title: Aberrant Dendritic Cell Subsets in Patients with Myasthenia Gravis and Related Clinical Features.

doi: 10.1159/000529626

Figure Lengend Snippet: Fig. 4. a–c Comparison of circulating dendritic cell subsets in patients with and without anti-AChR antibodies A total of 99 patients accepted the test for anti-AChR IgG antibodies. The circulating levels of pDCs, CD1c+ cDCs, and CD141+ cDCs in patients seropositive for anti-AChR IgG antibodies were compared with those found to be seronegative. As shown, there were no differences in the frequencies of pDCs, CD1c+ cDCs, and CD141+ cDCs in patients with and without anti-AChR antibodies.

Article Snippet: The section was incubated with 10% normal goat serum in phosphate-buffered saline at room temperature, followed by incubation with the primary antibody against CD303 (rabbit antihuman CD303, D161051-0100, Sango Biotech, Shanghai, China), CD1c (mouse anti-human CD1c, TA505384, Zhongshan Jinqiao Biotechnology Co., Ltd, Beijing, China), and CD141 (Rabbit antihuman CD141, BM4158, Boster Biological Technology, Wuhan, China).

Techniques: Comparison

Journal: Neuroimmunomodulation

Article Title: Aberrant Dendritic Cell Subsets in Patients with Myasthenia Gravis and Related Clinical Features.

doi: 10.1159/000529626

Figure Lengend Snippet: Fig. 5. Comparison of circulating dendritic cell subsets in patients with different MGFA (Myasthenia Gravis Foundation of America) classification as shown, there were no differences in the frequencies of pDCs (a), CD1c+ cDCs (b), and CD141+ cDCs (c) in patients with different MGFA classifications. Notably, patients with IVa and IVb were not included in the comparison because of the small sample size.

Article Snippet: The section was incubated with 10% normal goat serum in phosphate-buffered saline at room temperature, followed by incubation with the primary antibody against CD303 (rabbit antihuman CD303, D161051-0100, Sango Biotech, Shanghai, China), CD1c (mouse anti-human CD1c, TA505384, Zhongshan Jinqiao Biotechnology Co., Ltd, Beijing, China), and CD141 (Rabbit antihuman CD141, BM4158, Boster Biological Technology, Wuhan, China).

Techniques: Comparison

Journal: Journal of Inflammation Research

Article Title: Endothelial Injury-Induced Shedding of Thrombomodulin as a Biomarker for Predicting Adverse Prognosis in Sepsis

doi: 10.2147/JIR.S535854

Figure Lengend Snippet: The summary receiver operating characteristic (ROC) curves of the combination Thrombomodulin (TM), CD64 Index, SOFA Score, and APACHE II Score diagnostic effectiveness analysis for sepsis at 3 different time points. ( A ) The first day, the patient was diagnosed the sepsis (Day 1). ( B ) The third day, the patient was diagnosed the sepsis (Day 3). ( C) The seventh day, the patient was diagnosed the sepsis (Day 7).

Article Snippet: Primary antibodies - rabbit polyclonal anti-thrombomodulin (TM) antibody (HUABIO, #ET7107-92; 1:500) and mouse monoclonal anti-CD31 antibody (Abcam, # Ab182981 ; 1:200) - were applied to sufficiently cover the tissue and incubated overnight at 4°C.

Techniques: Diagnostic Assay

Journal: Journal of Inflammation Research

Article Title: Endothelial Injury-Induced Shedding of Thrombomodulin as a Biomarker for Predicting Adverse Prognosis in Sepsis

doi: 10.2147/JIR.S535854

Figure Lengend Snippet: Expression of plasma Thrombomodulin (TM) in mice in different groups at different time points. ** means p < 0.01, ***means p < 0.001, and ****means p <0.0001 versus in different groups.

Article Snippet: Primary antibodies - rabbit polyclonal anti-thrombomodulin (TM) antibody (HUABIO, #ET7107-92; 1:500) and mouse monoclonal anti-CD31 antibody (Abcam, # Ab182981 ; 1:200) - were applied to sufficiently cover the tissue and incubated overnight at 4°C.

Techniques: Expressing, Clinical Proteomics

Journal: Journal of Inflammation Research

Article Title: Endothelial Injury-Induced Shedding of Thrombomodulin as a Biomarker for Predicting Adverse Prognosis in Sepsis

doi: 10.2147/JIR.S535854

Figure Lengend Snippet: Immunofluorescence analysis of aortic endothelial integrity in septic mice. Immunostaining of mouse aortic sections showing CD31 (endothelial marker, red), thrombomodulin (TM, green), and DAPI (nuclear stain, blue) in Sham-operated (top) versus CLP-induced septic mice (bottom). Merged images demonstrate disrupted endothelial architecture and attenuated TM expression in the CLP group compared to intact endothelium in Sham controls, indicating sepsis-induced vascular injury. Scale bar: 100 μm. CLP: cecal ligation and puncture.

Article Snippet: Primary antibodies - rabbit polyclonal anti-thrombomodulin (TM) antibody (HUABIO, #ET7107-92; 1:500) and mouse monoclonal anti-CD31 antibody (Abcam, # Ab182981 ; 1:200) - were applied to sufficiently cover the tissue and incubated overnight at 4°C.

Techniques: Immunofluorescence, Immunostaining, Marker, Staining, Expressing, Ligation

Journal: Journal of Inflammation Research

Article Title: Endothelial Injury-Induced Shedding of Thrombomodulin as a Biomarker for Predicting Adverse Prognosis in Sepsis

doi: 10.2147/JIR.S535854

Figure Lengend Snippet: Expression of Thrombomodulin (TM) in aortic endothelial. ( A ) Expression efficiency of TM by quantitative real-time polymerase chain reaction (qRT-PCR). ( B ) Expression efficiency of TM by Western Blot. ( C ) Quantitated by densitometric analysis (n=6). ***means p < 0.001, and ****means p <0.0001 versus in different groups.

Article Snippet: Primary antibodies - rabbit polyclonal anti-thrombomodulin (TM) antibody (HUABIO, #ET7107-92; 1:500) and mouse monoclonal anti-CD31 antibody (Abcam, # Ab182981 ; 1:200) - were applied to sufficiently cover the tissue and incubated overnight at 4°C.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Western Blot