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Image Search Results
Journal: EMBO Reports
Article Title: An hepatitis B and D virus infection model using human pluripotent stem cell-derived hepatocytes
doi: 10.1038/s44319-024-00236-0
Figure Lengend Snippet: Reagents and tools table
Article Snippet: Rabbit anti-FoxA2 (1:400) was purchased from Cell Signaling, mouse anti-AFP (1:1000) from Sigma-Aldrich,
Techniques: Recombinant, Immunofluorescence, Western Blot, Sequencing, Membrane, Knock-Out, Transfection, Reverse Transcription, SYBR Green Assay, Protease Inhibitor, Diagnostic Assay, Software, Microscopy, Imaging
Journal: Oncology reports
Article Title: Molecular mechanism of LKB1 in the invasion and metastasis of colorectal cancer.
doi: 10.3892/or.2018.6877
Figure Lengend Snippet: Figure 1. Immunohistochemical staining of LKB1 in colorectal carcinoma tissue sections (magnification, x200). (A and B) Normal tissue (posi- tive). (C and D) Adenocarcinoma (negative). (E and F) Adenocarcinoma (positive). (G and H) Adenocarcinoma: Mucinous adenocarcinoma (positive). LKB1, liver kinase B1 gene.
Article Snippet: After blocking with 5% non-fat dry milk, the membranes were probed with primary antibodies [LKB1 (dilution 1:1,000);
Techniques: Immunohistochemical staining, Staining
Journal: Oncology reports
Article Title: Molecular mechanism of LKB1 in the invasion and metastasis of colorectal cancer.
doi: 10.3892/or.2018.6877
Figure Lengend Snippet: Figure 2. Effect of LKB1 siRNA on the morphological changes of LoVo cells magnification, x200). (A) Blank control group. (B) Negative control group. (C) LKB1 siRNA group. **P<0.01 vs. blank control group; ##P<0.01 vs. negative control group. LKB1, liver kinase B1 gene.
Article Snippet: After blocking with 5% non-fat dry milk, the membranes were probed with primary antibodies [LKB1 (dilution 1:1,000);
Techniques: Control, Negative Control
Journal: Oncology reports
Article Title: Molecular mechanism of LKB1 in the invasion and metastasis of colorectal cancer.
doi: 10.3892/or.2018.6877
Figure Lengend Snippet: Figure 5. Effect of metformin on the morphology of LoVo cells (magnifica- tion, x200). (A) Blank control group. (B) Metformin group. (C) LKB1 siRNA group. (D) LKB1 siRNA+metformin group. LKB1, liver kinase B1 gene.
Article Snippet: After blocking with 5% non-fat dry milk, the membranes were probed with primary antibodies [LKB1 (dilution 1:1,000);
Techniques: Control
Journal: Oncology reports
Article Title: Molecular mechanism of LKB1 in the invasion and metastasis of colorectal cancer.
doi: 10.3892/or.2018.6877
Figure Lengend Snippet: Figure 3. Western blot detection of LKB1 protein expression. (A) Blank control group. (B) Negative control group. (C) LKB1 siRNA group. **P<0.01 (LKB1), ##P<0.01 (p‑LKB1) vs. blank control group; XXP<0.01 (LKB1), IIP<0.01 (p‑LKB1) vs. negative control group. ΔΔP<0.01 (p‑LKB1/LKB1) vs. blank control group; ¤¤P<0.01 (p‑LKB1/LKB1) vs. negative control group. LKB1, liver kinase B1 gene; p, phosphorylated.
Article Snippet: After blocking with 5% non-fat dry milk, the membranes were probed with primary antibodies [LKB1 (dilution 1:1,000);
Techniques: Western Blot, Expressing, Control, Negative Control
Journal: Oncology reports
Article Title: Molecular mechanism of LKB1 in the invasion and metastasis of colorectal cancer.
doi: 10.3892/or.2018.6877
Figure Lengend Snippet: Figure 7. Invasion of CRC cells in the different groups (magnification, x200). (A) Blank control group. (B) Metformin group. (C) LKB1 siRNA group. (D) LKB1 siRNA+metformin group. **P<0.01 vs. blank control group, ##P<0.01 (p‑LKB1) vs. LKB1 siRNA group. LKB1, liver kinase B1 gene; p, phosphorylated; CRC, colorectal cancer.
Article Snippet: After blocking with 5% non-fat dry milk, the membranes were probed with primary antibodies [LKB1 (dilution 1:1,000);
Techniques: Control
Journal: Oncology reports
Article Title: Molecular mechanism of LKB1 in the invasion and metastasis of colorectal cancer.
doi: 10.3892/or.2018.6877
Figure Lengend Snippet: Figure 6. Migration of CRC cells in the different groups (magnifica- tion, x200). (A) Blank control group. (B) Metformin group. (C) LKB1 siRNA group. (D) LKB1 siRNA+metformin group. **P<0.01 vs. blank control group, ##P<0.01 (p‑LKB1) vs. LKB1 siRNA group. LKB1, liver kinase B1 gene; p, phosphorylated; CRC, colorectal cancer.
Article Snippet: After blocking with 5% non-fat dry milk, the membranes were probed with primary antibodies [LKB1 (dilution 1:1,000);
Techniques: Migration, Control
Journal: Oncology reports
Article Title: Molecular mechanism of LKB1 in the invasion and metastasis of colorectal cancer.
doi: 10.3892/or.2018.6877
Figure Lengend Snippet: Figure 9. Effects of metformin on the expression of AMPK and p‑AMPK in CRC LoVo cells. Western blot analysis showed the expression levels of AMPK and p‑AMPK in LoVo cells. (A) Blank control group. (B) Metformin group. (C) LKB1 siRNA group. (D) LKB1 siRNA+metformin group. **P<0.01 (AMPK) vs. blank control group, ##P<0.01 (p‑AMPK) vs. LKB1 siRNA group. AMPK, AMP‑activated protein kinase. ΔΔP<0.01 (p‑AMPK/AMPK) vs. blank control group; ¤¤P<0.01 (p‑AMPK/AMPK) vs. LKB1 siRNA group. LKB1, liver kinase B1 gene; AMPK, AMP‑activated protein kinase; p, phosphorylated; CRC, colorectal cancer.
Article Snippet: After blocking with 5% non-fat dry milk, the membranes were probed with primary antibodies [LKB1 (dilution 1:1,000);
Techniques: Expressing, Western Blot, Control
Journal: Oncology reports
Article Title: Molecular mechanism of LKB1 in the invasion and metastasis of colorectal cancer.
doi: 10.3892/or.2018.6877
Figure Lengend Snippet: Figure 8. Effects of metformin on the expression of LKB1 and p‑LKB1 in CRC LoVo cells. Western blot analysis showed the expression levels of LKB1 and p‑LKB1 in LoVo cells. (A) Blank control group. (B) Metformin group. (C) LKB1 siRNA group. (D) LKB1 siRNA+metformin group. **P<0.01 (LKB1) vs. blank control group, ##P<0.01 (p‑LKB1) vs. blank control group; XXP<0.01 (LKB1) vs. LKB1 siRNA group, IIP<0.01 (p‑LKB1) vs. LKB1 siRNA group. ΔΔP<0.01 (p‑LKB1/LKB1) vs. blank control group; ¤¤P<0.01 (p‑LKB1/LKB1) vs. LKB1 siRNA group. LKB1, liver kinase B1 gene; p, phosphorylated; CRC, colorectal cancer.
Article Snippet: After blocking with 5% non-fat dry milk, the membranes were probed with primary antibodies [LKB1 (dilution 1:1,000);
Techniques: Expressing, Western Blot, Control
Journal: Oncology reports
Article Title: Molecular mechanism of LKB1 in the invasion and metastasis of colorectal cancer.
doi: 10.3892/or.2018.6877
Figure Lengend Snippet: Figure 10. Effects of metformin on the expression of AKT and p‑AKT in CRC LoVo cells. Western blot analysis showed the expression levels of AKT and p‑AKT in LoVo cells. (A) Blank control group. (B) Metformin group. (C) LKB1 siRNA group. (D) LKB1 siRNA+metformin group. **P<0.01 (AKT) vs. blank control group, ##P<0.01 (p‑AKT) vs. LKB1 siRNA group.
Article Snippet: After blocking with 5% non-fat dry milk, the membranes were probed with primary antibodies [LKB1 (dilution 1:1,000);
Techniques: Expressing, Western Blot, Control