Journal: EMBO Reports

Article Title: An hepatitis B and D virus infection model using human pluripotent stem cell-derived hepatocytes

doi: 10.1038/s44319-024-00236-0

Figure Lengend Snippet: Reagents and tools table

Article Snippet: Rabbit anti-FoxA2 (1:400) was purchased from Cell Signaling, mouse anti-AFP (1:1000) from Sigma-Aldrich, mouse anti-ALB (1:1000) from Cedarlane, mouse anti-CD63 from Santa Cruz Biotechnology (1:400), rabbit anti-ADAR1 from Cell Signaling (1:1000), rabbit anti-LDLR from Abcam (1:200), rabbit anti-LAMP1 (1:200) and rabbit anti-EGFR (1:100) from Cell Signaling and rabbit anti-SCARB1 from Novus Biologicals (1:500).

Techniques: Recombinant, Immunofluorescence, Western Blot, Sequencing, Membrane, Knock-Out, Transfection, Reverse Transcription, SYBR Green Assay, Protease Inhibitor, Diagnostic Assay, Software, Microscopy, Imaging

Journal: iScience

Article Title: Coxsackievirus B infections are common in Cystic Fibrosis and experimental evidence supports protection by vaccination

doi: 10.1016/j.isci.2022.105070

Figure Lengend Snippet: Immune response to a CVB3 vaccine is in part T-cell-dependent but is mainly intact in F508del mice (A–C) Female TCRα ko (purple triangles; n = 13) and wt (black circles; n = 14) mice were vaccinated on two occasions (days 0 and 14) with a CVB3-field isolate vaccine (1.8 μg per dose, interscapular injection). (A) Experimental setup. (B) CVB3 neutralizing antibody (nAB) titers measured in the serum on days −2, 4, 5, 14, 21, and 28 after the initial vaccination by standard plaque neutralization assay. Individual mice are represented by single symbols. The horizontal line represents the mean ± SD ∗∗ p < 0.01, ∗∗∗∗ p < 0.0001 by two-way ANOVA with Sidak’s multiple comparison test. (C) nAB titer fluctuations after vaccination in the wt and TCRα KO mice visualized by an Akima spline. (D–F) Female F508del (green triangles; n = 5) and wt (black circles; n = 6) mice were vaccinated with a CVB3-field isolate vaccine (also used in a-c; 1.8 μg per dose, interscapular injection) on two occasions (days 0 and 14) as illustrated in (D). ICVB3 nAB titers were measured in the serum on days −2, 4, 5, 14, and 28 after the initial vaccination by standard plaque reduction assay. Individual mice are represented by single symbols. The horizontal line represents the mean ± SD. No statistical differences in nAB titers were found between the wt and F508del mice, two-way ANOVA with Sidak’s multiple comparison test. (F) Virus nAB titer fluctuations after vaccination with CVB3-field isolate vaccine in the wt and F508del mice visualized by an Akima spline. (G) Female and male F508del mice were vaccinated with CVB3-Nancy vaccine ( n = 4; green triangles; 1.8 μg per dose, interscapular injection) twice on days 0 and 14 as illustrated in (D). CVB3 nAB titers measured in the serum on days −2, 4, 5, 14, and 28 after the initial vaccination by standard plaque neutralization assay. Individual mice are represented by single symbols. The horizontal line represents the mean ± SD. (H) nAB titer fluctuations after vaccination with CVB3-Nancy vaccine in F508del mice visualized by an Akima spline. See also Figures S2 and .

Article Snippet: Coxsackievirus B3 antiserum , ATCC , V-030-501-560.

Techniques: Injection, Neutralization, Comparison, Virus

Journal: iScience

Article Title: Coxsackievirus B infections are common in Cystic Fibrosis and experimental evidence supports protection by vaccination

doi: 10.1016/j.isci.2022.105070

Figure Lengend Snippet: F508del mice raise virus neutralizing antibodies when vaccinated with monovalent CVB1 and CVB3 vaccines Female and male F508del mice were left untreated ( n = 1, CVB3 study), mock-vaccinated with buffer ( n = 3 for CVB3, n = 6 for CVB1), or vaccinated three times (on days 0, 21, and 35) with either CVB1 (CDC7; n = 6) vaccine (B and C), or CVB3 (Nancy; n = 8) vaccine (D and E), as illustrated by the vaccination schedule illustrated in (A). Each vaccine dose contained 1.8 μg of protein and was given by interscapular injection. The animals were infected with either CVB1 or CVB3 virus on day 63 after the initial vaccine dose and monitored until day 67. (B and D) Virus neutralizing antibody (nAB) titers in the serum of mice vaccinated with CVB1 vaccine (D; light teal triangle) or CVB3 vaccine (e; dark green triangle) on days 0, 21, 35, and 63 after the prime vaccination as measured by standard plaque neutralization assay. Individual animals are represented by individual symbols. The horizontal line represents the mean ± SD. (C and E) Fluctuations in virus neutralizing antibodies in CVB1- (C) and CVB3- (E) vaccinated mice as visualized by Akima Spline. See also Figure S4 .

Article Snippet: Coxsackievirus B3 antiserum , ATCC , V-030-501-560.

Techniques: Virus, Vaccines, Injection, Infection, Neutralization

Journal: iScience

Article Title: Coxsackievirus B infections are common in Cystic Fibrosis and experimental evidence supports protection by vaccination

doi: 10.1016/j.isci.2022.105070

Figure Lengend Snippet: CVB vaccines prevent acute CVB infections in F508del mice Female and male F508del mice were left untreated ( n = 1, CVB3 study; black circles), mock-vaccinated with vaccine buffer ( n = 6, CVB1 study; n = 3 CVB3 study; black circles), or vaccinated with CVB1 vaccine (A, C, and E; n = 6; light teal triangles) or CVB3 vaccine (B, D, and F; n = 8; dark teal triangles) as depicted in the schematic in Figure 3 A. On day 63 after the prime vaccination, the animals were infected with CVB1 (A, C, and E) or CVB3 (B, D, and F) and blood was collected on days 3 and 4 post-infection (p.i.). The experiment was terminated on day 4 p.i. and organs were collected. (A and B) Mean percentage body weight from the day of infection (day 0) in buffer-treated and CVB-vaccinated mice. Shown are the mean values ±SD ∗ p < 0.05 and ∗∗∗ p < 0.001 when comparing buffer versus vaccine at each time point by two-way ANOVA with Sidak’s multiple comparison test. ## p < 0.01 when comparing the time point with day 0 in each group by two-way ANOVA with Sidak’s multiple comparison test. (C and D) Replicating virus in the blood of untreated/buffer-treated, CVB1-vaccinated (C), or CVB3-vaccinated (D) mice as measured by standard plaque assay on days 3 and 4 p.i.. Each animal is represented by an individual symbol and the horizontal line represents the mean ± SD ∗ p < 0.05 and ∗∗ p < 0.01 as compared by the Mann–Whitney U test. (E and F) Titers of replicating virus particles in the organs of untreated/buffer-treated, CVB1-vaccinated (E), or CVB3-vaccinated (F) mice on day 4 p.i. measured by a standard plaque assay. Each animal is represented by an individual symbol and the horizontal line represents the mean ± SD ∗ p < 0.05 and ∗∗ p < 0.01 as compared by the Mann–Whitney U test.

Article Snippet: Coxsackievirus B3 antiserum , ATCC , V-030-501-560.

Techniques: Vaccines, Infection, Comparison, Virus, Plaque Assay, MANN-WHITNEY

Journal: iScience

Article Title: Coxsackievirus B infections are common in Cystic Fibrosis and experimental evidence supports protection by vaccination

doi: 10.1016/j.isci.2022.105070

Figure Lengend Snippet: Vaccination of F508del mice with CVB vaccines prevent virus-mediated organ damage F508del mice were buffer-treated ( n = 10) or vaccinated with CVB1 or CVB3 vaccines ( n = 13) and infected with CVB1 or CVB3 virus, as shown in Figure 3 A. Organs were collected on day 4 post-infection for histological analysis of organ integrity by haematoxylin and eosin (H&E) staining of formalin fixed paraffin embedded sections. (A) Pancreas scores of buffer + CVB or vaccine + CVB groups. Pancreas were scored according to exocrine damage and the presence of infiltrating immune cells. (B) Parenchymal health, (C) parenchymal damage and (D) hemosiderin laden particle staining in the lungs of buffer-treated + CVB-infected or vaccinated + CVB-infected mice. For (A)–(D), a score of 0 indicates healthy tissue and a score of 4 indicates highly damaged tissue. Each mouse is represented by an individual symbol and shown are the mean values ±SD ∗ p < 0.05, ∗∗∗∗ p < 0.001, as compared by the unpaired t -test. (E−L) Representative images of H&E-stained pancreas (E−H) and lung (I–L) from buffer + CVB1 (E and I), CVB1 vaccine + CVB1 (F and J), buffer + CVB3 (G and K), and CVB3 vaccine + CVB (H and L)-treated F508del mice on day 4 post-infection. In (I), the insert is a 5× magnification of the original image and the yellow arrows show hemosiderin laden particles. See also Figures S5 and .

Article Snippet: Coxsackievirus B3 antiserum , ATCC , V-030-501-560.

Techniques: Vaccines, Virus, Infection, Staining, Formalin-fixed Paraffin-Embedded

Journal: iScience

Article Title: Coxsackievirus B infections are common in Cystic Fibrosis and experimental evidence supports protection by vaccination

doi: 10.1016/j.isci.2022.105070

Figure Lengend Snippet:

Article Snippet: Coxsackievirus B3 antiserum , ATCC , V-030-501-560.

Techniques: Virus, Infection, Recombinant, Mutagenesis, Software

Journal: Journal of Neuroinflammation

Article Title: Involvement of neuronal IL-1β in acquired brain lesions in a rat model of neonatal encephalopathy

doi: 10.1186/1742-2094-10-110

Figure Lengend Snippet: List and features of antibodies

Article Snippet: Anti-PMN , Cedarlane - CLAD51140 , 1:200.

Techniques: