Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Super enhancer-driven LINC01013 mediates hypoxia-induced mitochondrial dysfunction by HSPA9 to determine pulmonary arterial smooth muscle cell fate

doi: 10.1007/s00018-025-06071-3

Figure Lengend Snippet: Silencing of LINC01013 inhibited hypoxia-induced hPASMC proliferation and inflammation. A , B hPASMC proliferation was determined by CCK8 and EdU incorporation assays ( n = 6). EdU (red), DAPI (blue), scale bar, 50 μm. C Western blotting analysis of PCNA, cyclin A and cyclin D in hPASMCs ( n = 6). D RT‒qPCR analysis showed the mRNA levels of TNF-α and IL-6 after LINC01013 knockdown, β-actin was used as a internal reference gene ( n = 6). E Western blotting analysis of TNF-α and IL-6 in hPASMCs. ( n = 6). All values are presented as the mean ± SEM. Statistical analysis was performed with one-way ANOVA. * p < 0.05, ** p < 0.01, *** p < 0.001. Nor, normoxia; Hyp, hypoxia; NC, negative control; si, siRNA

Article Snippet: The antibody against CEBPB (PB9171, BA0670, 1:500, Boster, Wuhan, China), H3K27ac (A7253, 1:500, ABclonal, Wuhan, China), H3K4me1 (A2355, 1:500, Wuhan, China), PCNA (A00125, 1:500, Boster, Wuhan, China), Cyclin A (PB0515, 1:500, Boster, Wuhan, China), Cyclin D (BM4272, 1:500, Boster, Wuhan, China), IL-6 (AF7236, 1:500, Beyotime, Shanghai, China), TNF-α (AF8208, 1:500, Beyotime, Shanghai, China), PKM2 (4053, 1:1000, Cell Signaling, MA, US), HK II (66974-1-Ig, 1:1000, Proteintech, IL, USA), PDH (2784, 1:1000, Cell Signaling, MA, US), HSPA9 (14887-1-AP, 1:5000, Proteintech, IL, USA), VDAC1 (10866-1-AP, 1:5000, Proteintech, IL, USA), and β-actin (TA-09, 1:1000, ZSGB‐BIO, Beijing, China) was incubated at 4 °C overnight, followed by incubation with appropriate horseradish peroxidase-conjugated secondary antibodies at room temperature for 1 h, and proteins were visualized with enhanced chemiluminescence reagents.

Techniques: Western Blot, Knockdown, Negative Control

Journal: Animal biotechnology

Article Title: Age-dependent changes in the expression and localization of LYZL4, LYZL6 and PCNA during testicular development in the Ashidan yak.

doi: 10.1080/10495398.2024.2344213

Figure Lengend Snippet: Figure 1. Relative expression of LYZL4, LYZL6 and PCNA mRNA in testis of the yak; different letters indicate significant differ ence (p < 0.01).

Article Snippet: The proteins were extracted from the testis following a previously established procedure.2 The protein was fixed in 12% Tricine SDS-PAGE for gel electrophoresis, then shifted onto PVDF membranes (Roche, Indianapolis, Indiana), after blocking of the membranes in 1 × 9 PBS and 0.1% Tween-20 containing 5% nonfat milk, then washed with PBS/Tween and membranes were incubated at 4°С for overnight with anti LYZL4 (1:1000, Bioss, Beijing, China), anti LYZL6 and anti PCNA antibody (1:1000, Aviva Systems Biology, San Diego, California) and anti β-actin (1:1000; Abcam, Cambridge, UK).

Techniques: Expressing

Journal: Animal biotechnology

Article Title: Age-dependent changes in the expression and localization of LYZL4, LYZL6 and PCNA during testicular development in the Ashidan yak.

doi: 10.1080/10495398.2024.2344213

Figure Lengend Snippet: Figure 2. Expression of LYZL4, LYZL6 and PCNA protein in tes tis of the yak; (A) the LYZL4, LYZL6 and PCNA detected by Western blotting; (B) The protein expression of LYZL4, LYZL6 and PCNA; different letters indicate significant difference (p < 0.05).

Article Snippet: The proteins were extracted from the testis following a previously established procedure.2 The protein was fixed in 12% Tricine SDS-PAGE for gel electrophoresis, then shifted onto PVDF membranes (Roche, Indianapolis, Indiana), after blocking of the membranes in 1 × 9 PBS and 0.1% Tween-20 containing 5% nonfat milk, then washed with PBS/Tween and membranes were incubated at 4°С for overnight with anti LYZL4 (1:1000, Bioss, Beijing, China), anti LYZL6 and anti PCNA antibody (1:1000, Aviva Systems Biology, San Diego, California) and anti β-actin (1:1000; Abcam, Cambridge, UK).

Techniques: Expressing, Western Blot

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Super enhancer-driven LINC01013 mediates hypoxia-induced mitochondrial dysfunction by HSPA9 to determine pulmonary arterial smooth muscle cell fate

doi: 10.1007/s00018-025-06071-3

Figure Lengend Snippet: Silencing of LINC01013 inhibited hypoxia-induced hPASMC proliferation and inflammation. A , B hPASMC proliferation was determined by CCK8 and EdU incorporation assays ( n = 6). EdU (red), DAPI (blue), scale bar, 50 μm. C Western blotting analysis of PCNA, cyclin A and cyclin D in hPASMCs ( n = 6). D RT‒qPCR analysis showed the mRNA levels of TNF-α and IL-6 after LINC01013 knockdown, β-actin was used as a internal reference gene ( n = 6). E Western blotting analysis of TNF-α and IL-6 in hPASMCs. ( n = 6). All values are presented as the mean ± SEM. Statistical analysis was performed with one-way ANOVA. * p < 0.05, ** p < 0.01, *** p < 0.001. Nor, normoxia; Hyp, hypoxia; NC, negative control; si, siRNA

Article Snippet: The antibody against CEBPB (PB9171, BA0670, 1:500, Boster, Wuhan, China), H3K27ac (A7253, 1:500, ABclonal, Wuhan, China), H3K4me1 (A2355, 1:500, Wuhan, China), PCNA (A00125, 1:500, Boster, Wuhan, China), Cyclin A (PB0515, 1:500, Boster, Wuhan, China), Cyclin D (BM4272, 1:500, Boster, Wuhan, China), IL-6 (AF7236, 1:500, Beyotime, Shanghai, China), TNF-α (AF8208, 1:500, Beyotime, Shanghai, China), PKM2 (4053, 1:1000, Cell Signaling, MA, US), HK II (66974-1-Ig, 1:1000, Proteintech, IL, USA), PDH (2784, 1:1000, Cell Signaling, MA, US), HSPA9 (14887-1-AP, 1:5000, Proteintech, IL, USA), VDAC1 (10866-1-AP, 1:5000, Proteintech, IL, USA), and β-actin (TA-09, 1:1000, ZSGB‐BIO, Beijing, China) was incubated at 4 °C overnight, followed by incubation with appropriate horseradish peroxidase-conjugated secondary antibodies at room temperature for 1 h, and proteins were visualized with enhanced chemiluminescence reagents.

Techniques: Western Blot, Knockdown, Negative Control

Journal: PLoS ONE

Article Title: Gastroprotective Mechanisms of the Monoterpene 1,8-Cineole (Eucalyptol)

doi: 10.1371/journal.pone.0134558

Figure Lengend Snippet: Animals were treated orally with 1% Tween-80 aqueous solution (control group), pantoprazole (40 mg/kg) or CIN (100 mg/kg) for 14 days. The filled arrow indicates the absence of the epithelial layer (ulcer area internal) and the dashed arrow indicates epithelial layer remaining (ulcer edge). Microphotographs depict the immunoreactivity for PCNA, Ki-67 and BrdU in the groups, magnification 200x (control) and 500x (CIN or pantoprazole).

Article Snippet: The slides were cooled to room temperature and endogenous peroxidase was blocked by the incubation in peroxidase blocking solution for 7.5 min. After cooling, the slides were incubated separately with the primary antibody for PCNA (anti-PCNA antibody [PC10]—Proliferation Marker (ab29)—Mouse monoclonal antibody, Abcan Inc, dilution 1:100, for 30 min) and with secondary antibody (Nichirei Biosciences Inc., dilution 1:200 for 30 min).

Techniques: Control