Journal: Kidney international

Article Title: Role of thymic- and graft-dependent mechanisms in tolerance induction to rat kidney transplant by donor PBMC infusion.

doi: 10.1038/sj.ki.5002202

Figure Lengend Snippet: Figure 3 | Immunofluorescence staining for CD4 þFoxp3 þ T cells in rat kidney allografts. Representative microphotographs of staining for CD4 (phycoerythrin-conjugated anti-rat CD4mAb, digitally converted in green to increase visibility) and Foxp3 (shown in red, anti-rat Foxp3mAb and Cy5-conjugated goat anti-rabbit as secondary antibody) on kidney tissue from BN-PBMC-infused rats killed (a) 5 days and (b) 60 days post-transplant and from an untreated rat killed 5 days post-transplant (c) Original magnification 400. Interstitial CD4 þ T cell infiltrates are shown. In kidney grafts from BN-PBMC rats (both at 5 and 60 days post-transplant), several CD4 þ T cells coexpress Foxp3 (indicated by arrows), whereas in the rejected kidney graft from untreated rats very few Foxp3 þ cells are present.

Article Snippet: Cells were stained with mouse anti-rat CD4, mouse anti-rat CD8 (OX-8), mouse anti-rat CD25, and mouse anti-rat MHCII (OX-6) mAbs (Serotec) followed by fluorescein isothiocyanate–goat antimouse immunoglobulin (Pharmingen, San Diego, CA, USA) as secondary antibody.

Techniques: Immunofluorescence, Staining

Journal: International Journal of Molecular Sciences

Article Title: Pathological Analysis of Ocular Lesions in a Murine Model of Sjögren’s Syndrome

doi: 10.3390/ijms18061209

Figure Lengend Snippet: Immune cell infiltration of target organs of the SS model mice. CD4, CD8, CD19, and F4/80 expressing immune cells were observed by confocal microscopy. ( a ) CD4 + T cells, ( b ) CD8 + T cells, ( c ) CD19 + B cells, and ( d ) F4/80 + macrophages in the lacrimal glands of the SS model mice were detected by Alexa-546-conjugated antibodies. Nuclei were stained with 4′,6-diamdino-2-phenylindole dihydrochloride (DAPI). The results are representative of five mice. Scale bar: 50 μm; ( e ) Flow cytometry analysis using lacrimal gland tissues from the SS model mice. CD4 and CD8 T cell subsets ware shown, and the result is representative of 5 mice; and ( f ) Cell numbers of CD4 + , CD8 + T cells, CD19 + B cells, and F4/8 + macrophages were calculated by flow cytometery analysis. Data are means ± SD of 5 mice.

Article Snippet: Lymphocytes from lacrimal gland tissues were stained with antibodies against PE-Cy7-conjugated anti-mouse CD4 mAb (TONBO Biosciences, San Diego, CA, USA), fluorescein isothiocyanate (FITC)-conjugated anti-mouse CD8 mAb (eBioscience, San Diego, CA, USA), PE-Cy5.5-conjugated anti-mouse CD19 mAb (TONBO Biosciences), and APC-Cy7-conjugated anti-mouse F4/80 mAb (BioLegend, San Diego, CA, USA).

Techniques: Expressing, Confocal Microscopy, Staining, Flow Cytometry

Journal: Cell Reports

Article Title: Klebsiella pneumoniae hijacks the Toll-IL-1R protein SARM1 in a type I IFN-dependent manner to antagonize host immunity

doi: 10.1016/j.celrep.2022.111167

Figure Lengend Snippet:

Article Snippet: CD4 , Fluidigm , Cat# 3145002B; RRID: AB_2687832.

Techniques: Virus, Recombinant, Purification, Enzyme-linked Immunosorbent Assay, Software

Journal: Nature Communications

Article Title: Fasting mimicking diet in mice delays cancer growth and reduces immunotherapy-associated cardiovascular and systemic side effects

doi: 10.1038/s41467-023-41066-3

Figure Lengend Snippet:

Article Snippet: Anti-mouse CD4, VioBright FITC (REA604) , Miltenyi Biotec , 130-118-692.

Techniques: In Vivo