Journal: Cell

Article Title: BMAL1-Driven Tissue Clocks Respond Independently to Light to Maintain Homeostasis.

doi: 10.1016/j.cell.2019.05.009

Figure Lengend Snippet: Figure 5. Epidermal BMAL1 Is Required to Prevent Increased Differentiation (A) Kaplan Meier survival curve for WT, RE, and KO mice (no significant difference between KO and RE, p = 0.2730). (B) Weight curve for WT, RE, and KO mice; data are represented as mean ± SD. (C) Epidermal cornification measured as thickness of cornified layer; p(RE versus WT) = 1.97 3 103, p(RE versus KO) = 1.33 3 106, p(WT versus KO) = 8.79 3 1010); data are represented as mean ± SD; scale bar, 100 mm.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-BMAL1 Abcam Cat# ab93806; RRID:AB_10675117 Rabbit anti-phosphorylated BMAL1 (Ser42) Cell Signaling Cat# 13936 Rabbit polyclonal anti-acetylated BMAL1 (Lys538) Merck Millipore Cat# AB15396; RRID:AB_1977054 Mouse monoclonal anti-ACTIN Sigma-Aldrich Cat# A5228; RRID:AB_262054 Mouse monoclonal anti-TUBULIN Sigma-Aldrich Cat# T7816; RRID:AB_261770 Rabbit polyclonal anti-RFP Rockland Cat# 600-401-379; RRID:AB_2209751 AffiniPure Goat anti-Rabbit IgG Jackson ImmunoResearch Labs Cat# 111-005-003; RRID:AB_2337913 BrightVision Poly-HRP-anti Goat IgG Biotin-free ImmunoLogic Cat# DPVG 55HRP Rabbit polyclonal anti-LORICRIN Abcam Cat# ab85679; RRID:AB_2134912 Chicken polyclonal anti-KERATIN 14 BioLegend Cat# 906001; RRID:AB_2565055 Goat anti-Chicken IgY, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-21449; RRID:AB_2535866 Goat anti-Rabbit IgG, Alexa Fluor 568 Thermo Fisher Scientific Cat# A-11036; RRID:AB_10563566 Chemicals, Peptides, and Recombinant Proteins SsoAdvanced Universal SYBR Green Supermix BioRad Cat# 1725270 Taqman Gene Expression Master Mix Thermo Fisher Cat# 4369016 Critical Commercial Assays Applied Biosystems High Capacity cDNA Reverse Transcription Kit Thermo Fisher Cat# 4368814 iScript cDNA Synthesis Kit BioRad Cat# 1708890 Deposited Data L/D RNA-sequencing dataset Gene Expression Omnibus GEO: GSE115104 D/D RNA-sequencing dataset Gene Expression Omnibus GEO: GSE114943 Experimental Models: Organisms/Strains Mouse:Wild Type –Bmal1wt/wt;K14-cretg/wt this paper N/A Mouse: Bmal1 knockout – Bmal1stopFL/stopFL; K14-crewt/wt this paper N/A Mouse: Bmal1 Epidermis-Reconstituted – Bmal1stopFL/stopFL; K14-cretg/wt this paper N/A Mouse:Wild Type –Bmal1wt/wt;Alfp-cretg/wt this paper N/A Mouse: Bmal1 knockout – Bmal1stopFL/stopFL; Alfp-crewt/wt this paper N/A Mouse: Bmal1 Hepatocyte-Reconstituted (Liver-RE) – Bmal1stopFL/stopFL; Alfp-cretg/wt this paper N/A Oligonucleotides qPCR Primers This paper Table S3 Software and Algorithms Jonckheere-Terpstra-Kendall (JTK_CYCLE) algorithm Hughes et al., 2010 N/A Trimmomatic (version 0.36) Bolger et al., 2014 N/A TopHat (version 2.1.1) Kim et al., 2013a https://ccb.jhu.edu/software/ tophat/index.shtml (Continued on next page) e1 Cell 177, 1436–1447.e1–e5, May 30, 2019

Techniques:

Journal:

Article Title: Increased expression of antimicrobial peptides and lysozyme in colonic epithelial cells of patients with ulcerative colitis

doi: 10.1046/j.1365-2249.2003.02035.x

Figure Lengend Snippet: Immunohistochemical staining for detection of HD-5 and lysozyme expressing cells in UC and control colon. (a) Control colon stained with anti-HD-5 antiserum. No stained cells could be detected. (b) UC colon stained with anti-HD-5 antiserum. Positive cells are seen at different locations in the crypt, but most commonly towards the crypt base. (c) Control colon stained with antilysozyme MoAb. Stained goblet cells as well as lamina propria macrophages can be seen. (d) UC colon stained with antilysozyme MoAb. Positive cells are seen at different locations in the crypt, but most commonly towards the crypt base. Original magnification: 55 × (a, d), and 220 × (b,c).

Article Snippet: Sections were incubated with antilysozyme MoAb BTI (Paesel + Lorei, Hanau, Germany) or anti-HD-5 polyclonal rabbit antiserum (kindly provided by Professor Tom Ganz, UCLA, Los Angeles, USA) diluted in PBS/BSA/saponin for 1 h at room temperature.

Techniques: Immunohistochemical staining, Staining, Expressing, Control