Journal: Oncotarget

Article Title: Deregulation of the miR-222-ABCG2 regulatory module in tongue squamous cell carcinoma contributes to chemoresistance and enhanced migratory/invasive potential

doi:

Figure Lengend Snippet: A. The IC50 values of DDP were detected by the CCK8 assay. Primary culture cells obtained from Case 6 had the highest IC50 values. B. Western blotting was used to detect the expression of chemoresistance markers (ABCG2 and ERCC1) and a metastasis-related gene (Slug). GAPDH was used as a loading control. The expression levels of ABCG2, ERCC1 and Slug were quantified; the results are shown in . C. Relative cell migration was measured by the transwell migration assay. Primary cells obtained from Case 6 had significantly higher migration ability than those from Cases 1, 3, 4 and 5. D. Relative cell invasion was measured by the transwell invasion assay. Primary cells obtained from Case 6 exhibited a significantly higher invasion ability than Cases 1, 2, 3 and 5. E. The expression of miR-222 was measured by qRT-PCR. Primary cells obtained from Case 6 showed significantly lower expression of miR-222 than those from Cases 1, 3 and 5. * p < 0.05 vs. Case 6.

Article Snippet: Western blots were performed as previously described [ ] using antibodies specific for ABCG2 (Santa Cruz, CA, USA), ERCC1 (Atlas Antibodies, Stockholm, Sweden), and Slug (Cell Signaling Technology, Beverly, MA); GAPDH (Sigma-Aldrich, MO, USA) was included as a control.

Techniques: CCK-8 Assay, Western Blot, Expressing, Control, Migration, Transwell Migration Assay, Transwell Invasion Assay, Quantitative RT-PCR

Journal: Oncotarget

Article Title: Deregulation of the miR-222-ABCG2 regulatory module in tongue squamous cell carcinoma contributes to chemoresistance and enhanced migratory/invasive potential

doi:

Figure Lengend Snippet: A. UM2 cells had significantly lower IC50 values than UM1 and CAL27 cells. B. Western blotting was used to detected the expression of ABCG2, ERCC1 and Slug in TSCC cell lines. The results from western blots were quantified; the results are shown in . (C and D) UM2 cells had significantly lower migration and invasion abilities than UM1 and CAL27 cells, as measured by transwell migration C. and invasion D. assays. E. UM2 cells showed significantly higher expression of miR-222 than UM1 and CAL27 cells, as measured by qRT-PCR. * p < 0.05 vs. UM2.

Article Snippet: Western blots were performed as previously described [ ] using antibodies specific for ABCG2 (Santa Cruz, CA, USA), ERCC1 (Atlas Antibodies, Stockholm, Sweden), and Slug (Cell Signaling Technology, Beverly, MA); GAPDH (Sigma-Aldrich, MO, USA) was included as a control.

Techniques: Western Blot, Expressing, Migration, Quantitative RT-PCR

Journal: Oncotarget

Article Title: Deregulation of the miR-222-ABCG2 regulatory module in tongue squamous cell carcinoma contributes to chemoresistance and enhanced migratory/invasive potential

doi:

Figure Lengend Snippet: Correlations among DDP resistance, migration/invasion potential and miR-222 expression in TSCC (Spearman)

Article Snippet: Western blots were performed as previously described [ ] using antibodies specific for ABCG2 (Santa Cruz, CA, USA), ERCC1 (Atlas Antibodies, Stockholm, Sweden), and Slug (Cell Signaling Technology, Beverly, MA); GAPDH (Sigma-Aldrich, MO, USA) was included as a control.

Techniques: Migration, Expressing

Journal: Oncotarget

Article Title: Deregulation of the miR-222-ABCG2 regulatory module in tongue squamous cell carcinoma contributes to chemoresistance and enhanced migratory/invasive potential

doi:

Figure Lengend Snippet: A. UM1 cells exhibited increased expression levels of ABCG2, ERCC1 and Slug proteins after transfection with ABCG2 cDNA, as detected by western blotting. B. UM1 cells had a significantly higher IC50 value after ABCG2 overexpression. (C and D) ABCG2 overexpression significantly increased the migration and invasion abilities of UM1 cells, as measured by transwell migration C. and invasion D. assays. * p < 0.05.

Article Snippet: Western blots were performed as previously described [ ] using antibodies specific for ABCG2 (Santa Cruz, CA, USA), ERCC1 (Atlas Antibodies, Stockholm, Sweden), and Slug (Cell Signaling Technology, Beverly, MA); GAPDH (Sigma-Aldrich, MO, USA) was included as a control.

Techniques: Expressing, Transfection, Western Blot, Over Expression, Migration

Journal: Oncotarget

Article Title: Deregulation of the miR-222-ABCG2 regulatory module in tongue squamous cell carcinoma contributes to chemoresistance and enhanced migratory/invasive potential

doi:

Figure Lengend Snippet: A. UM1 cells exhibited decreased levels of ABCG2, ERCC1 and Slug proteins following ABCG2 knockdown, as detected by western blotting. B. UM1 cells had a significantly lower IC50 value after transfection with ABCG2 siRNA. (C and D) ABCG2 knockdown significantly inhibited the migration and invasion abilities of UM1 cells, as measured by transwell migration C. and invasion D. assays. * p < 0.05.

Article Snippet: Western blots were performed as previously described [ ] using antibodies specific for ABCG2 (Santa Cruz, CA, USA), ERCC1 (Atlas Antibodies, Stockholm, Sweden), and Slug (Cell Signaling Technology, Beverly, MA); GAPDH (Sigma-Aldrich, MO, USA) was included as a control.

Techniques: Knockdown, Western Blot, Transfection, Migration

Journal: Oncotarget

Article Title: Deregulation of the miR-222-ABCG2 regulatory module in tongue squamous cell carcinoma contributes to chemoresistance and enhanced migratory/invasive potential

doi:

Figure Lengend Snippet: A. Predicted target sequences for miR-222 are located in the 3′-UTR of ABCG2 mRNA. B. Dual luciferase reporter assays were performed to evaluatethe target gene of miR-222. Following transfection with pGL-ABCG2 for 24 h, luciferase activity was significantly reduced in cells treated with miR-222 mimics relative to cells treated with control mimics (* p < 0.05 vs. blank and control). After the seed region of the target site was mutated (pGL-ABCG2 m), the effects of miR-222 on luciferase activity were abolished. C. Differential expression of miR-222 was tested by quantitative qRT-PCR in UM1 cells transfected with control mimics or miR-222 mimics and UM2 cells transfected with control LNA or miR-222 LNA (* p < 0.05). D. UM1 cells displayed decreased levels of ABCG2, ERCC1 and Slug proteins following treatment with miR-222 mimics, and UM2 cells display increased levels of ABCG2, ERCC1 and Slug proteins following treatment with miR-222 LNA.

Article Snippet: Western blots were performed as previously described [ ] using antibodies specific for ABCG2 (Santa Cruz, CA, USA), ERCC1 (Atlas Antibodies, Stockholm, Sweden), and Slug (Cell Signaling Technology, Beverly, MA); GAPDH (Sigma-Aldrich, MO, USA) was included as a control.

Techniques: Luciferase, Transfection, Activity Assay, Control, Quantitative Proteomics, Quantitative RT-PCR

Journal: Cancer Chemotherapy and Pharmacology

Article Title: A prospective study of biomarker-guided chemotherapy in patients with non-small cell lung cancer

doi: 10.1007/s00280-014-2513-x

Figure Lengend Snippet: A workflow chart showing a molecular signature tailored chemotherapy trial for the treatment for NSCLC. Flowchart and treatment algorithm used for selection of tailored chemotherapy based on molecular signature. Patients with ERCC1-negative or ERCC1-low were given platinum-based doublets; RRM1-negative or RRM1-low received either gemcitabine single agent or gemcitabine-based doublets; and patients with high β-tubulin III expression avoided taxanes

Article Snippet: Primary antibodies were as follows: mouse anti-ERCC1 antibody (Cat# 12A00409; ZSGB-Bio, Beijing, People’s Republic of China), rabbit anti-RRM1 antibody (Cat# 60073-1-lg; BJGB-Bio, Beijing, People’s Republic of China), and mouse anti-III β-tubulin antibody (Cat#1226P1011B; BJGB-Bio, Beijing, People’s Republic of China).

Techniques: Selection, Expressing