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Image Search Results
Journal: Breast Cancer Research : BCR
Article Title: BRCA1 mutation influences progesterone response in human benign mammary organoids
doi: 10.1186/s13058-019-1214-0
Figure Lengend Snippet: Confirmation and validation of ECM genes . a Confirmation of ECM genes was done by real-time PCR analysis of the samples used in the RNA-seq analysis. The data are fold changes of hormone treatment (dotted red line) and represent the mean ± SEM from three technical replicates (* p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0.001). b Validation of ECM genes was done by real-time PCR using new patient-derived individual BRCA1 mut and non-carrier organoids treated with E2+P4 and E2+P4+TPA. The data are fold changes of hormone treatment (dotted red line) and represent the mean ± SEM from three technical replicates (* p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0.001. c IHC staining of ECM proteins MMP10, COL1A1, COL3A1, and FN1 were performed in non-carrier and BRCA1 mut organoids. Scale bar, 100 μm
Article Snippet: The primary antibodies used were ER (1:200, Santa Cruz SC71064), PR (1:200, DAKO, M3568), pan cytokeratin (panCK; 1:200, Abcam, ab7753), Cytokeratin 18 (CK18; 1:200, Abcam, 93741), vimentin (Vim; 1:200, Abcam, 92547), α smooth muscle actin (αSMA; 1:200, Novus Biologicals, 600531), Cytokeratin 5/6 (CK5/6; 1:200, Agilent Technologies, M723729-2),
Techniques: Biomarker Discovery, Real-time Polymerase Chain Reaction, RNA Sequencing, Derivative Assay, Immunohistochemistry