Journal: Pharmaceutics

Article Title: The Potential of Cell-Penetrating Peptides for mRNA Delivery to Cancer Cells

doi: 10.3390/pharmaceutics14061271

Figure Lengend Snippet: Evaluation of CPP/mRNA-mediated protein expression. ( A ) Representative fluorescence images and a quantitative graph showing mCherry-positive CT26.CL25 cells 24 h after treatment with CPP/mCherry mRNA complexes (6.8 nM). Scale bar: 275 μm. Data are presented as the mean ± SD ( n = 3). ( B ) Representative flow cytometry histograms and graphs of EGFP-positive CL26.CL25 cells transfected with CPP/EGFP mRNA complexes (6.8 nM). Data are presented as the mean ± SD ( n = 3). ( C ) Western blot analysis of the expression levels of OVA and GAPDH in CT26.CL25 cells.

Article Snippet: The following western blot antibodies were used: OVA (Bio-Rad, #0220-1682, 1:1000), GAPDH (R&D, MAB5718, 1:1000), Anti-Rabbit IgG-HRP antibody (Gene Tex, GTX213110-01, 1:2000) and Anti-mouse IgG-HRP antibody (GebeTex, GTX213111-01, 1:2000).

Techniques: Expressing, Fluorescence, Flow Cytometry, Transfection, Western Blot

Journal: Animals : an Open Access Journal from MDPI

Article Title: Evaluation of a Novel Precision Biotic on Enterohepatic Health Markers and Growth Performance of Broiler Chickens under Enteric Challenge

doi: 10.3390/ani12192502

Figure Lengend Snippet: Target phenotypes used in analysis of intestinal T cells.

Article Snippet: CD4 , T-helper cells , MCA2164F Biorad.

Techniques: Marker

Journal: Vaccines

Article Title: Mannose-Modified Chitosan-Nanoparticle-Based Salmonella Subunit OralVaccine-Induced Immune Response and Efficacy in a Challenge Trial in Broilers

doi: 10.3390/vaccines8020299

Figure Lengend Snippet: Antibodies used for lymphocyte cell surface and intracellular IFNγ immunostaining.

Article Snippet: Rabbit anti-chicken IFNγ , Cat# AHP945Z; Bio-Rad; Hercules, CA, USA.

Techniques: Immunostaining