Journal: PLOS One

Article Title: Photobiomodulation therapy increases neural stem cell pool in aged 3xTg-AD mice

doi: 10.1371/journal.pone.0321668

Figure Lengend Snippet: A. Representative confocal images of Aβ plaques (6E10), microglia (Iba1) and CD68 showing diffuse microglial phagocytosis away from plaques in sham compared to localized phagocytosis around plaques in PBM-treated animals. B. Quantitative analysis of total and activated microglia show no overall differences in the neuroinflammatory environment surrounding Aβ plaques. (Unpaired t-test). C. Analysis of the distribution of CD68+ microglia shows a trend of higher concentration of phagocytic cells in contact with plaques, and decreasing phagocytosis away from plaques. (Two-way ANOVA).

Article Snippet: Up to six sections representing different regions of the hippocampus were immunostained with the following primary antibodies: Sox2 (Abcam), doublecortin (DCX, Santa Cruz), calretinin (CR, SWANT), β-amyloid (6E10, Biolegend), phospho-tau (pTau, AT8, Invitrogen), βIII-tubulin (Abcam), Iba1 (Synaptic Systems), and CD68 (Rockland).

Techniques: Concentration Assay

Journal: Journal of translational medicine

Article Title: The clinical importance of tumour-infiltrating macrophages and dendritic cells in periampullary adenocarcinoma differs by morphological subtype.

doi: 10.1186/s12967-017-1256-y

Figure Lengend Snippet: Fig. 1 Sample immunohistochemical images. Sample images of immunohistochemical staining of CD1a+ TIDC in a PB-type, b I-type tumour, CD68+ TAM in c PB-type, d I-type tumour, CD163+ TAM in e PB-type, f I-type tumour, and MARCO+ TAM in g PB-type, h I-type. Scale bar represents 20 μm

Article Snippet: For immunohistochemical (IHC) analysis of CD1a, CD68, CD163 and MARCO, 4 μm TMA-sections were pretreated using ULTRA Cell Conditioning Solution 1, pH 8.5 (Ventana Medical Systems Inc., Tucson, AZ, USA) for heat induced epitope retrieval, and stained in a Ventana BenchMark stainer (Ventana Medical Systems Inc.) with the following antibodies: CD1a: clone NCL-CD1a-220, diluted 1:25, LEICA Biosystems, Newcastle, UK, CD68: clone KP1, diluted 1:1000, Dako, Glostrup, Denmark, CD163: clone 10D6 diluted 1:200 Novus Biologicals, Abingdon, United Kingdom, MARCO clone HPA063793, diluted 1:250, Atlas Antibodies, Bromma, Sweden.

Techniques: Immunohistochemical staining, Staining

Journal: Journal of translational medicine

Article Title: The clinical importance of tumour-infiltrating macrophages and dendritic cells in periampullary adenocarcinoma differs by morphological subtype.

doi: 10.1186/s12967-017-1256-y

Figure Lengend Snippet: Fig. 3 Kaplan–Meier estimates of survival according to CD68+ TAM density. Kaplan–Meier estimates of 5-year overall survival according to high and low CD68+ TAM density in a the entire cohort, b in I-type tumours and c in PB-type tumours

Article Snippet: For immunohistochemical (IHC) analysis of CD1a, CD68, CD163 and MARCO, 4 μm TMA-sections were pretreated using ULTRA Cell Conditioning Solution 1, pH 8.5 (Ventana Medical Systems Inc., Tucson, AZ, USA) for heat induced epitope retrieval, and stained in a Ventana BenchMark stainer (Ventana Medical Systems Inc.) with the following antibodies: CD1a: clone NCL-CD1a-220, diluted 1:25, LEICA Biosystems, Newcastle, UK, CD68: clone KP1, diluted 1:1000, Dako, Glostrup, Denmark, CD163: clone 10D6 diluted 1:200 Novus Biologicals, Abingdon, United Kingdom, MARCO clone HPA063793, diluted 1:250, Atlas Antibodies, Bromma, Sweden.

Techniques:

Journal: The American journal of pathology

Article Title: The Versican G1 Fragment and Serum-Derived Hyaluronan-Associated Proteins Interact and Form a Complex in Granulation Tissue of Pressure Ulcers.

doi: 10.1016/j.ajpath.2017.10.015

Figure Lengend Snippet: Figure 9 Transient versican G1 fragmenteserum-derived hyaluronan-associated proteinehyaluronan (VG1F-SHAP-HA) complex formation in a mouse model of moist wound healing. A: Representative images from wound tissues from the moist wound-healing model are shown. Edematous granulation tissues were observed at day 3 after wounding; however, the granulation tissues were nonedematous and thin at days 1 and 6. The thickness of the granulation tissues is as follows: day 1, 0.77 0.10 mm; day 3, 2.55 0.17 mm; and day 6, 0.82 0.04 mm. B: Tissue specimens were stained with hematoxylin and eosin. C: Isolated tissues were stained by using the indicated probes. The VG1F-SHAP-HA complex was observed on day 3 in specific lesions, but was absent at days 1 and 6. The color of letters indicating molecules represents the color of the stain. The merged images indicate the VG1F-SHAP-HA complex. D: Immunohis- tochemical analysis of wound tissue taken from day 3. The specimen was stained with the indicated probes, and the merged images show VG1F colocalization, as detected by probes for HA, DPEAAE epitope, and macrophages (CD68). The merged images show macrophage/complex colocalization. Data are expressed as means SD (A). n Z 4 independent experiments (A). Scale bars Z 50 mm (BeD). HC, heavy chain.

Article Snippet: A goat pAb against bikunin (sc-21597) was purchased from Santa Cruz Biotechnology Inc. A mouse monoclonal antibody against the macrophage marker CD68 (STJ-6572) was purchased from St. John’s Laboratory Ltd (London, UK).

Techniques: Derivative Assay, Staining, Isolation

Journal: The American journal of pathology

Article Title: The Versican G1 Fragment and Serum-Derived Hyaluronan-Associated Proteins Interact and Form a Complex in Granulation Tissue of Pressure Ulcers.

doi: 10.1016/j.ajpath.2017.10.015

Figure Lengend Snippet: Figure 11 The versican G1 fragment (VG1F)eserum-derived hyaluronan (HA)eassociated proteineHA complex in human pressure ulcers. A: In human pressure ulcers, edematous wounds (e1 and e2) typically become flat and stabilized (f3 and f4) after appropriate treatments. B: The VG1F-HC complex was detected from the protein extract of wound surfaces shown in A (e1 and e2). The samples are indicated at the top of the figure and correspond to those shown in A. The samples were extracted with 6 mol/L GdnHCl and treated with trypsin and V8 protease (Materials and Methods); separated using SDS-PAGE under nonreducing conditions; and blotted. Incubation with the indicated antibodies revealed the cross-linked heavy chain (HC) and VG1 species (dagger). The bands of cross-linked HC and VG1 species (dagger) disappeared during wound healing. C: Paired samples from edematous or flat wounds were analyzed using Western blotting, and the bands shown in B were quantified. The relative intensities of the bands [VG1-HCs (daggers in B)]/the total polyclonal antibody 6084epositive bands (all VG1) differ significantly (P Z 0.02) between edematous and flat wounds. D: Direct immunofluorescence of wound surface samples obtained from different sites of one individual wound. Samples obtained from the edematous wound (green dashed circle) and flat wound (blue dashed circle) were fixed and incubated with VG1 and HC1 antibodies, as well as biotin-conjugated HAebinding protein, as indicated. A representative result from triplicate experiments with different samples is shown. E: Immunohistochemical analysis of pressure ulcer tissue. The specimen was stained, as indicated. The merged images show VG1F colocalization, as detected by antibodies for DPEAAE epitope, HC1, and macrophage CD68. Data are expressed as means SD (C). n Z 10 (C, each paired sample). Scale bars: 5 mm (D); 100 mm (E).

Article Snippet: A goat pAb against bikunin (sc-21597) was purchased from Santa Cruz Biotechnology Inc. A mouse monoclonal antibody against the macrophage marker CD68 (STJ-6572) was purchased from St. John’s Laboratory Ltd (London, UK).

Techniques: Derivative Assay, SDS Page, Incubation, Western Blot, Immunohistochemical staining, Staining

Journal: Cells

Article Title: Tofogliflozin Delays Portal Hypertension and Hepatic Fibrosis by Inhibiting Sinusoidal Capillarization in Cirrhotic Rats

doi: 10.3390/cells13060538

Figure Lengend Snippet: Effect of tofogliflozin on hepatic inflammation in cirrhotic rats. ( A ) Serum levels of aspartate transaminase (AST) and alanine transaminase (ALT). ( B , C ) ( B ) Hematoxylin and eosin (H&E) and ( C ) CD68 staining in the liver. Scale bar; 50 μm. ( D ) Quantification of CD68+-Kupffer cells. ( E , F ) Relative mRNA levels of ( E ) Adgre1 and ( F ) Tnfa , Il6, and Il1b in the liver. Gapdh was used as an internal control for qRT-PCR. Quantitative values are indicated as fold changes to the values of ( D ) Veh group or ( E , F ) C/O group. Data are the mean ± SD ( n = 10). * p < 0.05, ** p < 0.01, significant difference between groups determined by Student’s t -test. N.S, not significant; C/O, corn oil-injected negative control group; Veh, CCl 4 + vehicle-treated group; 10 mg, CCl 4 + tofogliflozin (10 mg/kg/day)-treated group; 20 mg, CCl 4 + tofogliflozin (20 mg/kg/day)-treated group.

Article Snippet: For immunohistochemical staining, liver tissue sections were blocked for 30 min following deparaffinization and antigen retrieval, then incubated overnight at 4 °C with mouse monoclonal CD68 antibody (1:100; GTX41868, GeneTex, Irvine, CA, USA).

Techniques: Staining, Control, Quantitative RT-PCR, Injection, Negative Control

Journal: Cell Reports Medicine

Article Title: An immunometabolism subtyping system identifies S100A9 + macrophage as an immune therapeutic target in colorectal cancer based on multiomics analysis

doi: 10.1016/j.xcrm.2023.100987

Figure Lengend Snippet:

Article Snippet: Rabbit monoclonal Anti-CD68 , Biolynx , Cat#BX50031.

Techniques: Staining, Control, Liposomes, Sequencing, Chromatography, RNA Sequencing, Software